Restriction map for
pF1KE2374
(Circular) (Six-base) MAPSORT of: pF1KE2374.seq Check: 2363 from: 1 to: 3965
pF1KE2374 3965 bp
With 182 enzymes: SgfI *
September 6, 2011 14:42 ..
AccI GT'mk_AC
Cuts at: 887 1003 887
Size: 116 3849
Acc65I G'GTAC_C
Cuts at: 981 981
Size: 3965
AclI AA'CG_TT
Cuts at: 3805 3805
Size: 3965
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1790 2222 2969 3237 1790
Size: 432 747 268 2518
Fragments arranged by size:
2518 747 432 268
AflIII A'CryG_T
Cuts at: 446 1317 2421 3398 446
Size: 871 1104 977 1013
Fragments arranged by size:
1104 1013 977 871
AgeI A'CCGG_T
Cuts at: 2353 2353
Size: 3965
AhdI GACnn_n'nnGTC
Cuts at: 186 828 186
Size: 642 3323
AlwNI CAG_nnn'CTG
Cuts at: 582 623 1270 2837 582
Size: 41 647 1567 1710
Fragments arranged by size:
1710 1567 647 41
ApaI G_GGCC'C
Cuts at: 163 163
Size: 3965
ApaLI G'TGCA_C
Cuts at: 475 799 2735 475
Size: 324 1936 1705
Fragments arranged by size:
1936 1705 324
ApoI r'AATT_y
Cuts at: 969 3318 3574 969
Size: 2349 256 1360
Fragments arranged by size:
2349 1360 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3965
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
AvaI C'yCGr_G
Cuts at: 162 610 722 833 985 162
Size: 448 112 111 152 3142
Fragments arranged by size:
3142 448 152 112 111
BamHI G'GATC_C
Cuts at: 264 990 264
Size: 726 3239
BanI G'GyrC_C
Cuts at: 981 1395 1643 1678 981
Size: 414 248 35 3268
Fragments arranged by size:
3268 414 248 35
BanII G_rGCy'C
Cuts at: 163 723 791 979 2009 163
Size: 560 68 188 1030 2119
Fragments arranged by size:
2119 1030 560 188 68
BbeI G_GCGC'C
Cuts at: 1399 1647 1399
Size: 248 3717
BbvCI CC'TCA_GC
Cuts at: 667 667
Size: 3965
BciVI GTATCCnnnnn_n'
Cuts at: 1863 2624 3965 1863
Size: 761 1341 1863
Fragments arranged by size:
1863 1341 761
BclI T'GATC_A
Cuts at: 1485 1485
Size: 3965
BglII A'GATC_T
Cuts at: 944 1480 3517 944
Size: 536 2037 1392
Fragments arranged by size:
2037 1392 536
BlpI GC'TnA_GC
Cuts at: 1077 1077
Size: 3965
Bme1580I G_kGCm'C
Cuts at: 163 479 803 1590 1683 2739 163
Size: 316 324 787 93 1056 1389
Fragments arranged by size:
1389 1056 787 324 316 93
BmrI ACTGGGnnnn_n'
Cuts at: 726 1177 1258 1593 3737 726
Size: 451 81 335 2144 954
Fragments arranged by size:
2144 954 451 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 3965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 879 574
Size: 305 3660
Bpu10I CC'TnA_GC
Cuts at: 667 667
Size: 3965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1141 2020 2512 2810 667
Size: 474 879 492 298 1822
Fragments arranged by size:
1822 879 492 474 298
BsaI GGTCTCn'nnnn_
Cuts at: 3669 3669
Size: 3965
BsaAI yAC'GTr
Cuts at: 449 795 1318 449
Size: 346 523 3096
Fragments arranged by size:
3096 523 346
BsaBI GATnn'nnATC
Cuts at: 6 931 1479 3516 6
Size: 925 548 2037 455
Fragments arranged by size:
2037 925 548 455
BsaHI Gr'CG_yC
Cuts at: 1396 1644 2346 3842 1396
Size: 248 702 1496 1519
Fragments arranged by size:
1519 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1154 1675 2353 2627 2774 3185 1154
Size: 521 678 274 147 411 1934
Fragments arranged by size:
1934 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 598 853 598
Size: 255 3710
BseYI C'CCAG_C
Cuts at: 153 175 504 872 1390 2148 2725 3221 153
Size: 22 329 368 518 758 577 496 897
Fragments arranged by size:
897 758 577 518 496 368 329 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 144
Size: 3965
BsiEI CG_ry'CG
Cuts at: 104 1507 1553 2761 104
Size: 1403 46 1208 1308
Fragments arranged by size:
1403 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 479 791 803 979 1757 1947 2739 479
Size: 312 12 176 778 190 792 1705
Fragments arranged by size:
1705 792 778 312 190 176 12
Bsp1286I G_dGCh'C
Cuts at: 163 479 723 791 803 979 1590 1683 1757
Size: 316 244 68 12 176 611 93 74
Cuts at: 1757 1947 2009 2739 163
Size: 190 62 730 1389
Fragments arranged by size:
1389 730 611 316 244 190 176 93
74 68 62 12
BspEI T'CCGG_A
Cuts at: 3185 3185
Size: 3965
BspHI T'CATG_A
Cuts at: 929 3141 929
Size: 2212 1753
BspMI ACCTGCnnnn'nnnn_
Cuts at: 833 948 1015 1229 1531 1912 833
Size: 115 67 214 302 381 2886
Fragments arranged by size:
2886 381 302 214 115 67
BsrBI CCG'CTC
Cuts at: 50 242 570 2257 2311 3795 3960 50
Size: 192 328 1687 54 1484 165 55
Fragments arranged by size:
1687 1484 328 192 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1877 1877
Size: 3965
BsrFI r'CCGG_y
Cuts at: 737 1963 2144 2353 737
Size: 1226 181 209 2349
Fragments arranged by size:
2349 1226 209 181
BssSI C'ACGA_G
Cuts at: 430 2236 2594 430
Size: 1806 358 1801
Fragments arranged by size:
1806 1801 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1342 1342
Size: 3965
BstBI TT'CG_AA
Cuts at: 2326 2326
Size: 3965
BstYI r'GATC_y
Cuts at: 1 264 326 944 990 1457 1480 1815 2061
Size: 263 62 618 46 467 23 335 246
Cuts at: 2061 3073 3159 3171 3517 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 618 467 449 346 335 263 246
86 62 46 23 12
BtgI C'CryG_G
Cuts at: 107 115 107
Size: 8 3957
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1915 2096 2259 1915
Size: 181 163 3621
Fragments arranged by size:
3621 181 163
BtsI GCAGTG_nn'
Cuts at: 479 764 1208 479
Size: 285 444 3236
Fragments arranged by size:
3236 444 285
DraI TTT'AAA
Cuts at: 964 964
Size: 3965
DraIII CAC_nnn'GTG
Cuts at: 261 261
Size: 3965
DrdI GACnn_nn'nnGTC
Cuts at: 1671 2529 1671
Size: 858 3107
EaeI y'GGCC_r
Cuts at: 1550 1724 2115 2142 3487 1550
Size: 174 391 27 1345 2028
Fragments arranged by size:
2028 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1550 1550
Size: 3965
EarI CTCTTCn'nnn_
Cuts at: 859 1988 2198 859
Size: 1129 210 2626
Fragments arranged by size:
2626 1129 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2173 2483 2629 3775 2173
Size: 310 146 1146 2363
Fragments arranged by size:
2363 1146 310 146
EcoICRI GAG'CTC
Cuts at: 789 977 789
Size: 188 3777
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 879 1790 2222 2969 3237 574
Size: 305 911 432 747 268 1302
Fragments arranged by size:
1302 911 747 432 305 268
EcoNI CCTnn'n_nnAGG
Cuts at: 379 409 379
Size: 30 3935
EcoO109I rG'GnC_Cy
Cuts at: 159 166 649 1104 159
Size: 7 483 455 3020
Fragments arranged by size:
3020 483 455 7
EcoRI G'AATT_C
Cuts at: 969 969
Size: 3965
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1984 1984
Size: 3965
FspI TGC'GCA
Cuts at: 1746 1746
Size: 3965
HaeII r_GCGC'y
Cuts at: 1399 1647 2669 3648 1399
Size: 248 1022 979 1716
Fragments arranged by size:
1716 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 172 204 268 300 814 846 936 968 1807
Size: 32 64 32 514 32 90 32 839
Cuts at: 1807 1837 1839 1869 172
Size: 30 2 30 2268
Fragments arranged by size:
2268 839 514 90 64 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1004 1004
Size: 3965
HindIII A'AGCT_T
Cuts at: 397 397
Size: 3965
KasI G'GCGC_C
Cuts at: 1395 1643 1395
Size: 248 3717
KpnI G_GTAC'C
Cuts at: 985 985
Size: 3965
MluI A'CGCG_T
Cuts at: 3398 3398
Size: 3965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2636 2820 2636
Size: 184 3781
MscI TGG'CCA
Cuts at: 1726 1726
Size: 3965
MslI CAynn'nnrTG
Cuts at: 133 407 1954 2081 133
Size: 274 1547 127 2017
Fragments arranged by size:
2017 1547 274 127
MspA1I CmG'CkG
Cuts at: 117 196 233 603 1076 1390 1750 2763 3008
Size: 79 37 370 473 314 360 1013 245
Cuts at: 3008 117
Size: 1074
Fragments arranged by size:
1074 1013 473 370 360 314 245 79
37
NaeI GCC'GGC
Cuts at: 2146 2146
Size: 3965
NarI GG'CG_CC
Cuts at: 1396 1644 1396
Size: 248 3717
NcoI C'CATG_G
Cuts at: 107 107
Size: 3965
NgoMIV G'CCGG_C
Cuts at: 2144 2144
Size: 3965
NspI r_CATG'y
Cuts at: 1018 1518 2425 1018
Size: 500 907 2558
Fragments arranged by size:
2558 907 500
PciI A'CATG_T
Cuts at: 2421 2421
Size: 3965
PfoI T'CCnGG_A
Cuts at: 589 589
Size: 3965
PmeI GTTT'AAAC
Cuts at: 964 964
Size: 3965
PmlI CAC'GTG
Cuts at: 449 449
Size: 3965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 3933
PpuMI rG'GwC_Cy
Cuts at: 649 649
Size: 3965
PspOMI G'GGCC_C
Cuts at: 159 159
Size: 3965
PspXI vC'TCGA_Gb
Cuts at: 610 610
Size: 3965
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 541 573 541
Size: 32 3933
PstI C_TGCA'G
Cuts at: 195 830 959 1012 1697 195
Size: 635 129 53 685 2463
Fragments arranged by size:
2463 685 635 129 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3965
PvuII CAG'CTG
Cuts at: 196 233 603 1390 1750 196
Size: 37 370 787 360 2411
Fragments arranged by size:
2411 787 370 360 37
RsrII CG'GwC_CG
Cuts at: 2160 2160
Size: 3965
SacI G_AGCT'C
Cuts at: 791 979 791
Size: 188 3777
SacII CC_GC'GG
Cuts at: 118 118
Size: 3965
SalI G'TCGA_C
Cuts at: 1002 1002
Size: 3965
SanDI GG'GwC_CC
Cuts at: 649 649
Size: 3965
SapI GCTCTTCn'nnn_
Cuts at: 1988 2198 1988
Size: 210 3755
SbfI CC_TGCA'GG
Cuts at: 1012 1012
Size: 3965
ScaI AGT'ACT
Cuts at: 3482 3482
Size: 3965
SfcI C'TryA_G
Cuts at: 33 191 826 955 1008 1693 2686 2877 33
Size: 158 635 129 53 685 993 191 1121
Fragments arranged by size:
1121 993 685 635 191 158 129 53
SfoI GGC'GCC
Cuts at: 1397 1645 1397
Size: 248 3717
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
SmaI CCC'GGG
Cuts at: 164 987 164
Size: 823 3142
SmlI C'TyrA_G
Cuts at: 610 682 1120 2035 2527 2789 610
Size: 72 438 915 492 262 1786
Fragments arranged by size:
1786 915 492 438 262 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3965
SphI G_CATG'C
Cuts at: 1018 1518 1018
Size: 500 3465
StyI C'CwwG_G
Cuts at: 107 215 1099 1453 107
Size: 108 884 354 2619
Fragments arranged by size:
2619 884 354 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1548 1681 2140 2349 1548
Size: 133 459 209 3164
Fragments arranged by size:
3164 459 209 133
TatI w'GTAC_w
Cuts at: 3480 3480
Size: 3965
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 3933
Tth111I GACn'n_nGTC
Cuts at: 1762 1762
Size: 3965
XbaI T'CTAG_A
Cuts at: 996 996
Size: 3965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 402 1460 402
Size: 1058 2907
XhoI C'TCGA_G
Cuts at: 610 610
Size: 3965
XmaI C'CCGG_G
Cuts at: 162 985 162
Size: 823 3142
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PshAI PsiI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI