Restriction map for
pF1KE2373
(Circular) (Six-base) MAPSORT of: pF1KE2373.seq Check: 3767 from: 1 to: 6542
pF1KE2373 6542 bp
With 182 enzymes: SgfI *
September 6, 2011 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2058 2641 3382 2058
Size: 583 741 5218
Fragments arranged by size:
5218 741 583
AccI GT'mk_AC
Cuts at: 3580 3580
Size: 6542
Acc65I G'GTAC_C
Cuts at: 3558 3558
Size: 6542
AclI AA'CG_TT
Cuts at: 6382 6382
Size: 6542
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 702 2527 4367 4799 5546 5814 652
Size: 50 1825 1840 432 747 268 1380
Fragments arranged by size:
1840 1825 1380 747 432 268 50
AfeI AGC'GCT
Cuts at: 383 1327 383
Size: 944 5598
AflIII A'CryG_T
Cuts at: 211 841 3134 3894 4998 5975 211
Size: 630 2293 760 1104 977 778
Fragments arranged by size:
2293 1104 977 778 760 630
AgeI A'CCGG_T
Cuts at: 4930 4930
Size: 6542
AleI CACnn'nnGTG
Cuts at: 688 1126 3508 688
Size: 438 2382 3722
Fragments arranged by size:
3722 2382 438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 496 2338 2477 496
Size: 1842 139 4561
Fragments arranged by size:
4561 1842 139
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2407 2439 2407
Size: 32 6510
AlwNI CAG_nnn'CTG
Cuts at: 468 630 702 999 2481 2676 2690 3003 3238
Size: 162 72 297 1482 195 14 313 235
Cuts at: 3238 3290 3409 3847 5414 468
Size: 52 119 438 1567 1596
Fragments arranged by size:
1596 1567 1482 438 313 297 235 195
162 119 72 52 14
ApaI G_GGCC'C
Cuts at: 1597 1806 2208 2268 3401 1597
Size: 209 402 60 1133 4738
Fragments arranged by size:
4738 1133 402 209 60
ApaLI G'TGCA_C
Cuts at: 732 5312 732
Size: 4580 1962
ApoI r'AATT_y
Cuts at: 1664 3546 5895 6151 1664
Size: 1882 2349 256 2055
Fragments arranged by size:
2349 2055 1882 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6542
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6542
AvaI C'yCGr_G
Cuts at: 2653 3562 2653
Size: 909 5633
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3014 3047 3014
Size: 33 6509
BamHI G'GATC_C
Cuts at: 1460 3567 1460
Size: 2107 4435
BanI G'GyrC_C
Cuts at: 690 1098 1392 2105 2159 2614 3558 3972 4220
Size: 408 294 713 54 455 944 414 248
Cuts at: 4220 4255 690
Size: 35 2977
Fragments arranged by size:
2977 944 713 455 414 408 294 248
54 35
BanII G_rGCy'C
Cuts at: 162 460 516 1597 1751 1806 1897 2208 2268
Size: 298 56 1081 154 55 91 311 60
Cuts at: 2268 3401 3556 4586 162
Size: 1133 155 1030 2118
Fragments arranged by size:
2118 1133 1081 1030 311 298 155 154
91 60 56 55
BbeI G_GCGC'C
Cuts at: 2163 2618 3976 4224 2163
Size: 455 1358 248 4481
Fragments arranged by size:
4481 1358 455 248
BbsI GAAGACnn'nnnn_
Cuts at: 1248 2043 2631 3445 1248
Size: 795 588 814 4345
Fragments arranged by size:
4345 814 795 588
BbvCI CC'TCA_GC
Cuts at: 1261 2743 3410 1261
Size: 1482 667 4393
Fragments arranged by size:
4393 1482 667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6508
BciVI GTATCCnnnnn_n'
Cuts at: 3015 4440 5201 6542 3015
Size: 1425 761 1341 3015
Fragments arranged by size:
3015 1425 1341 761
BclI T'GATC_A
Cuts at: 4062 4062
Size: 6542
BfrBI ATG'CAT
Cuts at: 1415 1415
Size: 6542
BglI GCCn_nnn'nGGC
Cuts at: 1011 1107 1583 1592 2117 3188 1011
Size: 96 476 9 525 1071 4365
Fragments arranged by size:
4365 1071 525 476 96 9
BglII A'GATC_T
Cuts at: 4057 6094 4057
Size: 2037 4505
BlpI GC'TnA_GC
Cuts at: 2469 3654 2469
Size: 1185 5357
Bme1580I G_kGCm'C
Cuts at: 695 736 1597 1806 2208 2268 2952 2967 3401
Size: 41 861 209 402 60 684 15 434
Cuts at: 3401 4167 4260 5316 695
Size: 766 93 1056 1921
Fragments arranged by size:
1921 1056 861 766 684 434 402 209
93 60 41 15
BmgBI CAC'GTC
Cuts at: 1160 1160
Size: 6542
BmrI ACTGGGnnnn_n'
Cuts at: 478 597 690 915 3754 3835 4170 6314 478
Size: 119 93 225 2839 81 335 2144 706
Fragments arranged by size:
2839 2144 706 335 225 119 93 81
BmtI G_CTAG'C
Cuts at: 1849 2899 1849
Size: 1050 5492
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4909 4909
Size: 6542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 976 1050 1225 1312 1421 2032 2337 2698
Size: 658 74 175 87 109 611 305 361
Cuts at: 2698 2782 2794 2905 3025 3370 3418 318
Size: 84 12 111 120 345 48 3442
Fragments arranged by size:
3442 658 611 361 345 305 175 120
111 109 87 84 74 48 12
Bpu10I CC'TnA_GC
Cuts at: 1261 2482 2743 3055 3410 1261
Size: 1221 261 312 355 4393
Fragments arranged by size:
4393 1221 355 312 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1006 3718 4597 5089 5387 310
Size: 696 2712 879 492 298 1465
Fragments arranged by size:
2712 1465 879 696 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 140 1432 2758 6246 140
Size: 1292 1326 3488 436
Fragments arranged by size:
3488 1326 1292 436
BsaAI yAC'GTr
Cuts at: 3534 3895 3534
Size: 361 6181
BsaBI GATnn'nnATC
Cuts at: 6 1885 4056 6093 6
Size: 1879 2171 2037 455
Fragments arranged by size:
2171 2037 1879 455
BsaHI Gr'CG_yC
Cuts at: 558 986 2160 2555 2615 3973 4221 4923 6419
Size: 428 1174 395 60 1358 248 702 1496
Cuts at: 6419 558
Size: 681
Fragments arranged by size:
1496 1358 1174 702 681 428 395 248
60
BsaWI w'CCGG_w
Cuts at: 1406 1480 3521 3731 4252 4930 5204 5351 5762
Size: 74 2041 210 521 678 274 147 411
Cuts at: 5762 1406
Size: 2186
Fragments arranged by size:
2186 2041 678 521 411 274 210 147
74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2335 2365 2723 2753 2335
Size: 30 358 30 6124
Fragments arranged by size:
6124 358 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 104 358 1240 1249 2140 2308 2350 2731 3022
Size: 254 882 9 891 168 42 381 291
Cuts at: 3022 3025 3028 3031 104
Size: 3 3 3 3615
Fragments arranged by size:
3615 891 882 381 291 254 168 42
9 3 3 3
BseYI C'CCAG_C
Cuts at: 163 378 737 910 1322 1328 1850 2200 2376
Size: 215 359 173 412 6 522 350 176
Cuts at: 2376 2517 2954 2993 3159 3967 4725 5302 5798
Size: 141 437 39 166 808 758 577 496
Cuts at: 5798 163
Size: 907
Fragments arranged by size:
907 808 758 577 522 496 437 412
359 350 215 176 173 166 141 39
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 2086 2254 2608 2647 1330
Size: 756 168 354 39 5225
Fragments arranged by size:
5225 756 354 168 39
BsiEI CG_ry'CG
Cuts at: 104 2008 4084 4130 5338 104
Size: 1904 2076 46 1208 1308
Fragments arranged by size:
2076 1904 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 641 736 841 1925 3556 4334 4524 5316 641
Size: 95 105 1084 1631 778 190 792 1867
Fragments arranged by size:
1867 1631 1084 792 778 190 105 95
BsmBI CGTCTCn'nnnn_
Cuts at: 202 2699 202
Size: 2497 4045
Bsp1286I G_dGCh'C
Cuts at: 162 460 516 641 695 736 841 1597 1751
Size: 298 56 125 54 41 105 756 154
Cuts at: 1751 1806 1897 1925 2208 2268 2952 2967 3401
Size: 55 91 28 283 60 684 15 434
Cuts at: 3401 3556 4167 4260 4334 4524 4586 5316 162
Size: 155 611 93 74 190 62 730 1388
Fragments arranged by size:
1388 756 730 684 611 434 298 283
190 155 154 125 105 93 91 74
62 60 56 55 54 41 28 15
BspEI T'CCGG_A
Cuts at: 1406 1480 5762 1406
Size: 74 4282 2186
Fragments arranged by size:
4282 2186 74
BspHI T'CATG_A
Cuts at: 5718 5718
Size: 6542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 710 2058 2208 2641 3319 3382 3592 3806 4108
Size: 1348 150 433 678 63 210 214 302
Cuts at: 4108 4489 710
Size: 381 2763
Fragments arranged by size:
2763 1348 678 433 381 302 214 210
150 63
BsrBI CCG'CTC
Cuts at: 50 582 957 1239 4834 4888 6372 6537 50
Size: 532 375 282 3595 54 1484 165 55
Fragments arranged by size:
3595 1484 532 375 282 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1086 1430 4454 1086
Size: 344 3024 3174
Fragments arranged by size:
3174 3024 344
BsrFI r'CCGG_y
Cuts at: 658 667 2831 2972 4540 4721 4930 658
Size: 9 2164 141 1568 181 209 2270
Fragments arranged by size:
2270 2164 1568 209 181 141 9
BsrGI T'GTAC_A
Cuts at: 3281 3281
Size: 6542
BssSI C'ACGA_G
Cuts at: 2290 4813 5171 2290
Size: 2523 358 3661
Fragments arranged by size:
3661 2523 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1530 2105 3919 1530
Size: 575 1814 4153
Fragments arranged by size:
4153 1814 575
BstBI TT'CG_AA
Cuts at: 4903 4903
Size: 6542
BstEII G'GTnAC_C
Cuts at: 1243 2026 1243
Size: 783 5759
BstXI CCAn_nnnn'nTGG
Cuts at: 777 3045 3178 777
Size: 2268 133 4141
Fragments arranged by size:
4141 2268 133
BstYI r'GATC_y
Cuts at: 1 824 1460 3567 4034 4057 4392 4638 5650
Size: 823 636 2107 467 23 335 246 1012
Cuts at: 5650 5736 5748 6094 1
Size: 86 12 346 449
Fragments arranged by size:
2107 1012 823 636 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1195 1232 1195
Size: 37 6505
BtgI C'CryG_G
Cuts at: 485 1183 2187 485
Size: 698 1004 4840
Fragments arranged by size:
4840 1004 698
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 582 1914 3342 4492 4673 4836 582
Size: 1332 1428 1150 181 163 2288
Fragments arranged by size:
2288 1428 1332 1150 181 163
BtsI GCAGTG_nn'
Cuts at: 1342 3465 3785 1342
Size: 2123 320 4099
Fragments arranged by size:
4099 2123 320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 843 878 1413 1448 843
Size: 35 535 35 5937
Fragments arranged by size:
5937 535 35 35
DraI TTT'AAA
Cuts at: 3541 3541
Size: 6542
DraIII CAC_nnn'GTG
Cuts at: 645 645
Size: 6542
DrdI GACnn_nn'nnGTC
Cuts at: 1024 1252 4248 5106 1024
Size: 228 2996 858 2460
Fragments arranged by size:
2996 2460 858 228
EaeI y'GGCC_r
Cuts at: 386 537 563 656 767 851 1040 1364 2005
Size: 151 26 93 111 84 189 324 641
Cuts at: 2005 2147 2543 2870 3047 4127 4301 4692 4719
Size: 142 396 327 177 1080 174 391 27
Cuts at: 4719 6064 386
Size: 1345 864
Fragments arranged by size:
1345 1080 864 641 396 391 327 324
189 177 174 151 142 111 93 84
27 26
EagI C'GGCC_G
Cuts at: 2005 4127 2005
Size: 2122 4420
EarI CTCTTCn'nnn_
Cuts at: 167 239 1066 1699 2047 4565 4775 167
Size: 72 827 633 348 2518 210 1934
Fragments arranged by size:
2518 1934 827 633 348 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 496 762 1162 1288 1600 4750 5060 5206 6352
Size: 266 400 126 312 3150 310 146 1146
Cuts at: 6352 496
Size: 686
Fragments arranged by size:
3150 1146 686 400 312 310 266 146
126
EcoICRI GAG'CTC
Cuts at: 3554 3554
Size: 6542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 652 702 976 1050 1225 1312 1421 2032
Size: 334 50 274 74 175 87 109 611
Cuts at: 2032 2337 2527 2698 2782 2794 2905 3025 3370
Size: 305 190 171 84 12 111 120 345
Cuts at: 3370 3418 4367 4799 5546 5814 318
Size: 48 949 432 747 268 1046
Fragments arranged by size:
1046 949 747 611 432 345 334 305
274 268 190 175 171 120 111 109
87 84 74 50 48 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3058 3058
Size: 6542
EcoO109I rG'GnC_Cy
Cuts at: 930 1594 1722 1802 1803 1821 1838 2204 2265
Size: 664 128 80 1 18 17 366 61
Cuts at: 2265 2478 2526 3145 3384 3398 3458 3681 930
Size: 213 48 619 239 14 60 223 3791
Fragments arranged by size:
3791 664 619 366 239 223 213 128
80 61 60 48 18 17 14 1
EcoRI G'AATT_C
Cuts at: 3546 3546
Size: 6542
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4561 4561
Size: 6542
FspI TGC'GCA
Cuts at: 4323 4323
Size: 6542
HaeII r_GCGC'y
Cuts at: 385 999 1286 1329 1355 1973 2163 2253 2262
Size: 614 287 43 26 618 190 90 9
Cuts at: 2262 2618 3976 4224 5246 6225 385
Size: 356 1358 248 1022 979 702
Fragments arranged by size:
1358 1022 979 702 618 614 356 287
248 190 90 43 26 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 780 812 1491 1523 2335 2367 4384 4414 4416
Size: 32 679 32 812 32 2017 30 2
Cuts at: 4416 4446 780
Size: 30 2876
Fragments arranged by size:
2876 2017 812 679 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3581 3581
Size: 6542
HindIII A'AGCT_T
Cuts at: 2428 2428
Size: 6542
KasI G'GCGC_C
Cuts at: 2159 2614 3972 4220 2159
Size: 455 1358 248 4481
Fragments arranged by size:
4481 1358 455 248
KpnI G_GTAC'C
Cuts at: 3562 3562
Size: 6542
MluI A'CGCG_T
Cuts at: 211 5975 211
Size: 5764 778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2943 5213 5397 2159
Size: 784 2270 184 3304
Fragments arranged by size:
3304 2270 784 184
MscI TGG'CCA
Cuts at: 539 565 769 2149 3049 4303 539
Size: 26 204 1380 900 1254 2778
Fragments arranged by size:
2778 1380 1254 900 204 26
MslI CAynn'nnrTG
Cuts at: 688 1033 1126 1714 3139 3508 4531 4658 688
Size: 345 93 588 1425 369 1023 127 2572
Fragments arranged by size:
2572 1425 1023 588 369 345 127 93
MspA1I CmG'CkG
Cuts at: 487 903 1185 1265 1569 1854 2010 2277 2451
Size: 416 282 80 304 285 156 267 174
Cuts at: 2451 2461 2496 2541 2954 2979 3003 3653 3967
Size: 10 35 45 413 25 24 650 314
Cuts at: 3967 4327 5340 5585 487
Size: 360 1013 245 1444
Fragments arranged by size:
1444 1013 650 416 413 360 314 304
285 282 267 245 174 156 80 45
35 25 24 10
NaeI GCC'GGC
Cuts at: 4723 4723
Size: 6542
NarI GG'CG_CC
Cuts at: 2160 2615 3973 4221 2160
Size: 455 1358 248 4481
Fragments arranged by size:
4481 1358 455 248
NgoMIV G'CCGG_C
Cuts at: 4721 4721
Size: 6542
NheI G'CTAG_C
Cuts at: 1845 2895 1845
Size: 1050 5492
NotI GC'GGCC_GC
Cuts at: 2005 2005
Size: 6542
NsiI A_TGCA'T
Cuts at: 1417 1417
Size: 6542
NspI r_CATG'y
Cuts at: 3138 3334 3595 4095 5002 3138
Size: 196 261 500 907 4678
Fragments arranged by size:
4678 907 500 261 196
PasI CC'CwG_GG
Cuts at: 2386 3403 2386
Size: 1017 5525
PciI A'CATG_T
Cuts at: 3134 4998 3134
Size: 1864 4678
PflMI CCAn_nnn'nTGG
Cuts at: 648 3394 648
Size: 2746 3796
PfoI T'CCnGG_A
Cuts at: 334 1658 1790 2411 334
Size: 1324 132 621 4465
Fragments arranged by size:
4465 1324 621 132
PmeI GTTT'AAAC
Cuts at: 3541 3541
Size: 6542
PmlI CAC'GTG
Cuts at: 3534 3534
Size: 6542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 828 860 5681 5713 828
Size: 32 4821 32 1657
Fragments arranged by size:
4821 1657 32 32
PpuMI rG'GwC_Cy
Cuts at: 930 1722 1838 2478 3145 930
Size: 792 116 640 667 4327
Fragments arranged by size:
4327 792 667 640 116
PshAI GACnn'nnGTC
Cuts at: 1444 3445 1444
Size: 2001 4541
PspOMI G'GGCC_C
Cuts at: 1593 1802 2204 2264 3397 1593
Size: 209 402 60 1133 4738
Fragments arranged by size:
4738 1133 402 209 60
PstI C_TGCA'G
Cuts at: 311 575 626 1535 1700 2333 2456 2894 3455
Size: 264 51 909 165 633 123 438 561
Cuts at: 3455 3589 4274 311
Size: 134 685 2579
Fragments arranged by size:
2579 909 685 633 561 438 264 165
134 123 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6542
PvuII CAG'CTG
Cuts at: 1854 2277 2451 2496 2541 2979 3003 3967 4327
Size: 423 174 45 45 438 24 964 360
Cuts at: 4327 1854
Size: 4069
Fragments arranged by size:
4069 964 438 423 360 174 45 45
24
RsrII CG'GwC_CG
Cuts at: 4737 4737
Size: 6542
SacI G_AGCT'C
Cuts at: 3556 3556
Size: 6542
SacII CC_GC'GG
Cuts at: 488 1186 488
Size: 698 5844
SalI G'TCGA_C
Cuts at: 3579 3579
Size: 6542
SanDI GG'GwC_CC
Cuts at: 1722 1838 3145 1722
Size: 116 1307 5119
Fragments arranged by size:
5119 1307 116
SapI GCTCTTCn'nnn_
Cuts at: 1066 1699 2047 4565 4775 1066
Size: 633 348 2518 210 2833
Fragments arranged by size:
2833 2518 633 348 210
SbfI CC_TGCA'GG
Cuts at: 3589 3589
Size: 6542
ScaI AGT'ACT
Cuts at: 6059 6059
Size: 6542
SexAI A'CCwGG_T
Cuts at: 641 2780 641
Size: 2139 4403
SfcI C'TryA_G
Cuts at: 33 307 571 622 1531 1696 2232 2329 2452
Size: 274 264 51 909 165 536 97 123
Cuts at: 2452 2890 3451 3585 4270 5263 5454 33
Size: 438 561 134 685 993 191 1121
Fragments arranged by size:
1121 993 909 685 561 536 438 274
264 191 165 134 123 97 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 1583 1592 1583
Size: 9 6533
SfoI GGC'GCC
Cuts at: 2161 2616 3974 4222 2161
Size: 455 1358 248 4481
Fragments arranged by size:
4481 1358 455 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6542
SmaI CCC'GGG
Cuts at: 3564 3564
Size: 6542
SmlI C'TyrA_G
Cuts at: 325 1021 3697 4612 5104 5366 325
Size: 696 2676 915 492 262 1501
Fragments arranged by size:
2676 1501 915 696 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6542
SphI G_CATG'C
Cuts at: 3334 3595 4095 3334
Size: 261 500 5781
Fragments arranged by size:
5781 500 261
StuI AGG'CCT
Cuts at: 178 3475 178
Size: 3297 3245
StyI C'CwwG_G
Cuts at: 179 3038 3676 4030 179
Size: 2859 638 354 2691
Fragments arranged by size:
2859 2691 638 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 751 1320 1336 1845 2052 3516 4125 4258 4717
Size: 569 16 509 207 1464 609 133 459
Cuts at: 4717 4926 751
Size: 209 2367
Fragments arranged by size:
2367 1464 609 569 509 459 209 207
133 16
TatI w'GTAC_w
Cuts at: 3281 6057 3281
Size: 2776 3766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1931 1963 6059 6091 1931
Size: 32 4096 32 2382
Fragments arranged by size:
4096 2382 32 32
Tth111I GACn'n_nGTC
Cuts at: 1158 4339 1158
Size: 3181 3361
XbaI T'CTAG_A
Cuts at: 3573 3573
Size: 6542
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1905 2400 2922 4037 1905
Size: 495 522 1115 4410
Fragments arranged by size:
4410 1115 522 495
XmaI C'CCGG_G
Cuts at: 3562 3562
Size: 6542
XmnI GAAnn'nnTTC
Cuts at: 3487 3487
Size: 6542
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BsiWI BsmI BssHII
BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NruI PacI PsiI PspXI PsrI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI