Restriction map for
pF1KE2370
(Circular) (Six-base) MAPSORT of: pF1KE2370.seq Check: 3946 from: 1 to: 3632
pF1KE2370 3632 bp
With 182 enzymes: SgfI *
August 18, 2011 15:44 ..
AccI GT'mk_AC
Cuts at: 670 670
Size: 3632
Acc65I G'GTAC_C
Cuts at: 648 648
Size: 3632
AclI AA'CG_TT
Cuts at: 3472 3472
Size: 3632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1457 1889 2636 2904 1457
Size: 432 747 268 2185
Fragments arranged by size:
2185 747 432 268
AflIII A'CryG_T
Cuts at: 984 2088 3065 984
Size: 1104 977 1551
Fragments arranged by size:
1551 1104 977
AgeI A'CCGG_T
Cuts at: 2020 2020
Size: 3632
AlwNI CAG_nnn'CTG
Cuts at: 937 2504 937
Size: 1567 2065
ApaI G_GGCC'C
Cuts at: 155 155
Size: 3632
ApaLI G'TGCA_C
Cuts at: 2402 2402
Size: 3632
ApoI r'AATT_y
Cuts at: 636 2985 3241 636
Size: 2349 256 1027
Fragments arranged by size:
2349 1027 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3632
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
AvaI C'yCGr_G
Cuts at: 652 652
Size: 3632
BamHI G'GATC_C
Cuts at: 657 657
Size: 3632
BanI G'GyrC_C
Cuts at: 648 1062 1310 1345 648
Size: 414 248 35 2935
Fragments arranged by size:
2935 414 248 35
BanII G_rGCy'C
Cuts at: 155 646 1676 155
Size: 491 1030 2111
Fragments arranged by size:
2111 1030 491
BbeI G_GCGC'C
Cuts at: 1066 1314 1066
Size: 248 3384
BciVI GTATCCnnnnn_n'
Cuts at: 1530 2291 3632 1530
Size: 761 1341 1530
Fragments arranged by size:
1530 1341 761
BclI T'GATC_A
Cuts at: 1152 1152
Size: 3632
BglII A'GATC_T
Cuts at: 1147 3184 1147
Size: 2037 1595
BlpI GC'TnA_GC
Cuts at: 744 744
Size: 3632
Bme1580I G_kGCm'C
Cuts at: 155 1257 1350 2406 155
Size: 1102 93 1056 1381
Fragments arranged by size:
1381 1102 1056 93
BmgBI CAC'GTC
Cuts at: 114 114
Size: 3632
BmrI ACTGGGnnnn_n'
Cuts at: 844 925 1260 3404 844
Size: 81 335 2144 1072
Fragments arranged by size:
2144 1072 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1687 2179 2477 808
Size: 879 492 298 1963
Fragments arranged by size:
1963 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3336 3336
Size: 3632
BsaAI yAC'GTr
Cuts at: 985 985
Size: 3632
BsaBI GATnn'nnATC
Cuts at: 6 1146 3183 6
Size: 1140 2037 455
Fragments arranged by size:
2037 1140 455
BsaHI Gr'CG_yC
Cuts at: 1063 1311 2013 3509 1063
Size: 248 702 1496 1186
Fragments arranged by size:
1496 1186 702 248
BsaWI w'CCGG_w
Cuts at: 821 1342 2020 2294 2441 2852 821
Size: 521 678 274 147 411 1601
Fragments arranged by size:
1601 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 600 630 600
Size: 30 3602
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 613 613
Size: 3632
BseYI C'CCAG_C
Cuts at: 163 1057 1815 2392 2888 163
Size: 894 758 577 496 907
Fragments arranged by size:
907 894 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 394
Size: 3632
BsiEI CG_ry'CG
Cuts at: 104 309 1174 1220 2428 104
Size: 205 865 46 1208 1308
Fragments arranged by size:
1308 1208 865 205 46
BsiHKAI G_wGCw'C
Cuts at: 646 1424 1614 2406 646
Size: 778 190 792 1872
Fragments arranged by size:
1872 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 155 646 1257 1350 1424 1614 1676 2406 155
Size: 491 611 93 74 190 62 730 1381
Fragments arranged by size:
1381 730 611 491 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2852 2852
Size: 3632
BspHI T'CATG_A
Cuts at: 2808 2808
Size: 3632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 221 682 896 1198 1579 221
Size: 461 214 302 381 2274
Fragments arranged by size:
2274 461 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1924 1978 3462 3627 50
Size: 1874 54 1484 165 55
Fragments arranged by size:
1874 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1544 1544
Size: 3632
BsrFI r'CCGG_y
Cuts at: 167 1630 1811 2020 167
Size: 1463 181 209 1779
Fragments arranged by size:
1779 1463 209 181
BssSI C'ACGA_G
Cuts at: 1903 2261 1903
Size: 358 3274
BstAPI GCAn_nnn'nTGC
Cuts at: 1009 1009
Size: 3632
BstBI TT'CG_AA
Cuts at: 1993 1993
Size: 3632
BstYI r'GATC_y
Cuts at: 1 392 542 657 1124 1147 1482 1728 2740
Size: 391 150 115 467 23 335 246 1012
Cuts at: 2740 2826 2838 3184 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 391 346 335 246 150
115 86 23 12
BtgI C'CryG_G
Cuts at: 107 396 426 107
Size: 289 30 3313
Fragments arranged by size:
3313 289 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1582 1763 1926 1582
Size: 181 163 3288
Fragments arranged by size:
3288 181 163
BtsI GCAGTG_nn'
Cuts at: 875 875
Size: 3632
DraI TTT'AAA
Cuts at: 631 631
Size: 3632
DrdI GACnn_nn'nnGTC
Cuts at: 319 1338 2196 319
Size: 1019 858 1755
Fragments arranged by size:
1755 1019 858
EaeI y'GGCC_r
Cuts at: 1217 1391 1782 1809 3154 1217
Size: 174 391 27 1345 1695
Fragments arranged by size:
1695 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1217 1217
Size: 3632
EarI CTCTTCn'nnn_
Cuts at: 268 1655 1865 268
Size: 1387 210 2035
Fragments arranged by size:
2035 1387 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1840 2150 2296 3442 1840
Size: 310 146 1146 2030
Fragments arranged by size:
2030 1146 310 146
EcoICRI GAG'CTC
Cuts at: 644 644
Size: 3632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1457 1889 2636 2904 1457
Size: 432 747 268 2185
Fragments arranged by size:
2185 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 151 152 195 357 771 151
Size: 1 43 162 414 3012
Fragments arranged by size:
3012 414 162 43 1
EcoRI G'AATT_C
Cuts at: 636 636
Size: 3632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1651 1651
Size: 3632
FspI TGC'GCA
Cuts at: 1413 1413
Size: 3632
HaeII r_GCGC'y
Cuts at: 417 1066 1314 2336 3315 417
Size: 649 248 1022 979 734
Fragments arranged by size:
1022 979 734 649 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 1474 1504 1506 1536 286
Size: 32 1156 30 2 30 2382
Fragments arranged by size:
2382 1156 32 30 30 2
HincII GTy'rAC
Cuts at: 671 671
Size: 3632
KasI G'GCGC_C
Cuts at: 1062 1310 1062
Size: 248 3384
KpnI G_GTAC'C
Cuts at: 652 652
Size: 3632
MluI A'CGCG_T
Cuts at: 3065 3065
Size: 3632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 329 497 2303 2487 329
Size: 168 1806 184 1474
Fragments arranged by size:
1806 1474 184 168
MscI TGG'CCA
Cuts at: 1393 1393
Size: 3632
MslI CAynn'nnrTG
Cuts at: 112 586 1621 1748 112
Size: 474 1035 127 1996
Fragments arranged by size:
1996 1035 474 127
MspA1I CmG'CkG
Cuts at: 743 1057 1417 2430 2675 743
Size: 314 360 1013 245 1700
Fragments arranged by size:
1700 1013 360 314 245
NaeI GCC'GGC
Cuts at: 169 1813 169
Size: 1644 1988
NarI GG'CG_CC
Cuts at: 1063 1311 1063
Size: 248 3384
NcoI C'CATG_G
Cuts at: 107 107
Size: 3632
NgoMIV G'CCGG_C
Cuts at: 167 1811 167
Size: 1644 1988
NspI r_CATG'y
Cuts at: 522 685 1185 2092 522
Size: 163 500 907 2062
Fragments arranged by size:
2062 907 500 163
PasI CC'CwG_GG
Cuts at: 147 147
Size: 3632
PciI A'CATG_T
Cuts at: 2088 2088
Size: 3632
PmeI GTTT'AAAC
Cuts at: 631 631
Size: 3632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 3600
PpuMI rG'GwC_Cy
Cuts at: 357 357
Size: 3632
PspOMI G'GGCC_C
Cuts at: 151 151
Size: 3632
PstI C_TGCA'G
Cuts at: 679 1364 679
Size: 685 2947
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3632
PvuII CAG'CTG
Cuts at: 1057 1417 1057
Size: 360 3272
RsrII CG'GwC_CG
Cuts at: 1827 1827
Size: 3632
SacI G_AGCT'C
Cuts at: 646 646
Size: 3632
SalI G'TCGA_C
Cuts at: 669 669
Size: 3632
SapI GCTCTTCn'nnn_
Cuts at: 1655 1865 1655
Size: 210 3422
SbfI CC_TGCA'GG
Cuts at: 679 679
Size: 3632
ScaI AGT'ACT
Cuts at: 3149 3149
Size: 3632
SfcI C'TryA_G
Cuts at: 33 675 1360 2353 2544 33
Size: 642 685 993 191 1121
Fragments arranged by size:
1121 993 685 642 191
SfoI GGC'GCC
Cuts at: 1064 1312 1064
Size: 248 3384
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
SmaI CCC'GGG
Cuts at: 654 654
Size: 3632
SmlI C'TyrA_G
Cuts at: 787 1702 2194 2456 787
Size: 915 492 262 1963
Fragments arranged by size:
1963 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3632
SphI G_CATG'C
Cuts at: 522 685 1185 522
Size: 163 500 2969
Fragments arranged by size:
2969 500 163
StyI C'CwwG_G
Cuts at: 107 766 1120 107
Size: 659 354 2619
Fragments arranged by size:
2619 659 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 580 1215 1348 1807 2016 580
Size: 635 133 459 209 2196
Fragments arranged by size:
2196 635 459 209 133
TatI w'GTAC_w
Cuts at: 3147 3147
Size: 3632
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 600 632 3149 3181 600
Size: 32 2517 32 1051
Fragments arranged by size:
2517 1051 32 32
Tth111I GACn'n_nGTC
Cuts at: 1429 1429
Size: 3632
XbaI T'CTAG_A
Cuts at: 663 663
Size: 3632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1127 1127
Size: 3632
XmaI C'CCGG_G
Cuts at: 652 652
Size: 3632
XmnI GAAnn'nnTTC
Cuts at: 343 343
Size: 3632
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI