Restriction map for
pF1KE2360
(Circular) (Six-base) MAPSORT of: pF1KE2360.seq Check: 689 from: 1 to: 9332
pF1KE2360 9332 bp
With 182 enzymes: SgfI *
September 30, 2015 09:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3741 3741
Size: 9332
AccI GT'mk_AC
Cuts at: 364 1358 3459 4651 6370 364
Size: 994 2101 1192 1719 3326
Fragments arranged by size:
3326 2101 1719 1192 994
Acc65I G'GTAC_C
Cuts at: 2385 6348 2385
Size: 3963 5369
AclI AA'CG_TT
Cuts at: 1203 5238 9172 1203
Size: 4035 3934 1363
Fragments arranged by size:
4035 3934 1363
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3220 3472 4192 4286 4334 4475 4496 5382 6341
Size: 252 720 94 48 141 21 886 959
Cuts at: 6341 7157 7589 8336 8604 3220
Size: 816 432 747 268 3948
Fragments arranged by size:
3948 959 886 816 747 720 432 268
252 141 94 48 21
AfeI AGC'GCT
Cuts at: 3205 3205
Size: 9332
AflIII A'CryG_T
Cuts at: 4470 4538 6062 6258 6684 7788 8765 4470
Size: 68 1524 196 426 1104 977 5037
Fragments arranged by size:
5037 1524 1104 977 426 196 68
AgeI A'CCGG_T
Cuts at: 7720 7720
Size: 9332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 373 5990 373
Size: 5617 3715
AlwNI CAG_nnn'CTG
Cuts at: 2179 2430 4312 5020 6637 8204 2179
Size: 251 1882 708 1617 1567 3307
Fragments arranged by size:
3307 1882 1617 1567 708 251
ApaI G_GGCC'C
Cuts at: 396 396
Size: 9332
ApaLI G'TGCA_C
Cuts at: 1633 2590 8102 1633
Size: 957 5512 2863
Fragments arranged by size:
5512 2863 957
ApoI r'AATT_y
Cuts at: 124 744 1499 1529 1602 2113 2596 3310 5351
Size: 620 755 30 73 511 483 714 2041
Cuts at: 5351 5489 6073 6162 6336 8685 8941 124
Size: 138 584 89 174 2349 256 515
Fragments arranged by size:
2349 2041 755 714 620 584 515 511
483 256 174 138 89 73 30
AseI AT'TA_AT
Cuts at: 20 4402 5837 20
Size: 4382 1435 3515
Fragments arranged by size:
4382 3515 1435
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9332
AvaI C'yCGr_G
Cuts at: 3062 3079 6352 3062
Size: 17 3273 6042
Fragments arranged by size:
6042 3273 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3589 3622 4606 4639 3589
Size: 33 984 33 8282
Fragments arranged by size:
8282 984 33 33
BamHI G'GATC_C
Cuts at: 6357 6357
Size: 9332
BanI G'GyrC_C
Cuts at: 2385 6348 6762 7010 7045 2385
Size: 3963 414 248 35 4672
Fragments arranged by size:
4672 3963 414 248 35
BanII G_rGCy'C
Cuts at: 167 199 233 396 1292 1399 1438 2654 4037
Size: 32 34 163 896 107 39 1216 1383
Cuts at: 4037 4958 6346 7376 167
Size: 921 1388 1030 2123
Fragments arranged by size:
2123 1388 1383 1216 1030 921 896 163
107 39 34 32
BbeI G_GCGC'C
Cuts at: 6766 7014 6766
Size: 248 9084
BbsI GAAGACnn'nnnn_
Cuts at: 557 1113 1799 5665 6227 557
Size: 556 686 3866 562 3662
Fragments arranged by size:
3866 3662 686 562 556
BciVI GTATCCnnnnn_n'
Cuts at: 3921 6041 7230 7991 9332 3921
Size: 2120 1189 761 1341 3921
Fragments arranged by size:
3921 2120 1341 1189 761
BclI T'GATC_A
Cuts at: 6852 6852
Size: 9332
BfrBI ATG'CAT
Cuts at: 1041 3553 5367 6231 1041
Size: 2512 1814 864 4142
Fragments arranged by size:
4142 2512 1814 864
BglI GCCn_nnn'nGGC
Cuts at: 1433 1433
Size: 9332
BglII A'GATC_T
Cuts at: 294 2580 6847 8884 294
Size: 2286 4267 2037 742
Fragments arranged by size:
4267 2286 2037 742
BlpI GC'TnA_GC
Cuts at: 139 161 4926 6444 139
Size: 22 4765 1518 3027
Fragments arranged by size:
4765 3027 1518 22
Bme1580I G_kGCm'C
Cuts at: 396 805 1637 1928 2148 2594 6957 7050 8106
Size: 409 832 291 220 446 4363 93 1056
Cuts at: 8106 396
Size: 1622
Fragments arranged by size:
4363 1622 1056 832 446 409 291 220
93
BmgBI CAC'GTC
Cuts at: 5919 5919
Size: 9332
BmrI ACTGGGnnnn_n'
Cuts at: 3909 6544 6625 6960 9104 3909
Size: 2635 81 335 2144 4137
Fragments arranged by size:
4137 2635 2144 335 81
BmtI G_CTAG'C
Cuts at: 3194 6026 3194
Size: 2832 6500
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 489 1769 2906 3844 7699 489
Size: 1280 1137 938 3855 2122
Fragments arranged by size:
3855 2122 1280 1137 938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 4900 5215 2351
Size: 2549 315 6468
Fragments arranged by size:
6468 2549 315
Bpu10I CC'TnA_GC
Cuts at: 4957 4957
Size: 9332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1706 1786 2561 5300 5517 6508 7387 7879
Size: 889 80 775 2739 217 991 879 492
Cuts at: 7879 8177 817
Size: 298 1972
Fragments arranged by size:
2739 1972 991 889 879 775 492 298
217 80
BsaI GGTCTCn'nnnn_
Cuts at: 504 9036 504
Size: 8532 800
BsaAI yAC'GTr
Cuts at: 447 5799 6685 447
Size: 5352 886 3094
Fragments arranged by size:
5352 3094 886
BsaBI GATnn'nnATC
Cuts at: 6 2837 3404 4424 6846 8883 6
Size: 2831 567 1020 2422 2037 455
Fragments arranged by size:
2831 2422 2037 1020 567 455
BsaHI Gr'CG_yC
Cuts at: 779 6763 7011 7713 9209 779
Size: 5984 248 702 1496 902
Fragments arranged by size:
5984 1496 902 702 248
BsaWI w'CCGG_w
Cuts at: 1044 6521 7042 7720 7994 8141 8552 1044
Size: 5477 521 678 274 147 411 1824
Fragments arranged by size:
5477 1824 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 880 910 880
Size: 30 9302
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1112 1745 2879 5898 1112
Size: 633 1134 3019 4546
Fragments arranged by size:
4546 3019 1134 633
BseYI C'CCAG_C
Cuts at: 2897 5155 6757 7515 8092 8588 2897
Size: 2258 1602 758 577 496 3641
Fragments arranged by size:
3641 2258 1602 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 1604 2766 3698 4560 4598 5986 379
Size: 1225 1162 932 862 38 1388 3725
Fragments arranged by size:
3725 1388 1225 1162 932 862 38
BsiEI CG_ry'CG
Cuts at: 104 6874 6920 8128 104
Size: 6770 46 1208 1308
Fragments arranged by size:
6770 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 167 1637 2594 2654 2889 3757 4037 4958 6346
Size: 1470 957 60 235 868 280 921 1388
Cuts at: 6346 7124 7314 8106 167
Size: 778 190 792 1393
Fragments arranged by size:
1470 1393 1388 957 921 868 792 778
280 235 190 60
BsmI GAATG_Cn'
Cuts at: 1685 6231 1685
Size: 4546 4786
BsmBI CGTCTCn'nnnn_
Cuts at: 669 952 669
Size: 283 9049
Bsp1286I G_dGCh'C
Cuts at: 167 199 233 396 805 1292 1399 1438 1637
Size: 32 34 163 409 487 107 39 199
Cuts at: 1637 1928 2148 2594 2654 2889 3757 4037 4958
Size: 291 220 446 60 235 868 280 921
Cuts at: 4958 6346 6957 7050 7124 7314 7376 8106 167
Size: 1388 611 93 74 190 62 730 1393
Fragments arranged by size:
1393 1388 921 868 730 611 487 446
409 291 280 235 220 199 190 163
107 93 74 62 60 39 34 32
BspEI T'CCGG_A
Cuts at: 1044 8552 1044
Size: 7508 1824
BspHI T'CATG_A
Cuts at: 2696 8508 2696
Size: 5812 3520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2178 3643 3741 5958 6382 6596 6898 7279 2178
Size: 1465 98 2217 424 214 302 381 4231
Fragments arranged by size:
4231 2217 1465 424 381 302 214 98
BsrBI CCG'CTC
Cuts at: 50 6114 7624 7678 9162 9327 50
Size: 6064 1510 54 1484 165 55
Fragments arranged by size:
6064 1510 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 2500 2607 3479 5147 6092 7244 2096
Size: 404 107 872 1668 945 1152 4184
Fragments arranged by size:
4184 1668 1152 945 872 404 107
BsrFI r'CCGG_y
Cuts at: 7330 7511 7720 7330
Size: 181 209 8942
Fragments arranged by size:
8942 209 181
BssSI C'ACGA_G
Cuts at: 7603 7961 7603
Size: 358 8974
BstAPI GCAn_nnn'nTGC
Cuts at: 2430 4164 6709 2430
Size: 1734 2545 5053
Fragments arranged by size:
5053 2545 1734
BstBI TT'CG_AA
Cuts at: 486 4176 7693 486
Size: 3690 3517 2125
Fragments arranged by size:
3690 3517 2125
BstYI r'GATC_y
Cuts at: 1 294 2580 2687 3279 6357 6824 6847 7182
Size: 293 2286 107 592 3078 467 23 335
Cuts at: 7182 7428 8440 8526 8538 8884 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
3078 2286 1012 592 467 449 346 335
293 246 107 86 23 12
BstZ17I GTA'TAC
Cuts at: 4652 4652
Size: 9332
Bsu36I CC'TnA_GG
Cuts at: 2891 3284 4028 2891
Size: 393 744 8195
Fragments arranged by size:
8195 744 393
BtgI C'CryG_G
Cuts at: 107 200 2681 2786 107
Size: 93 2481 105 6653
Fragments arranged by size:
6653 2481 105 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5038 7282 7463 7626 5038
Size: 2244 181 163 6744
Fragments arranged by size:
6744 2244 181 163
BtsI GCAGTG_nn'
Cuts at: 3106 4865 6575 3106
Size: 1759 1710 5863
Fragments arranged by size:
5863 1759 1710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1266 1301 1266
Size: 35 9297
DraI TTT'AAA
Cuts at: 2666 4379 4568 5843 6331 2666
Size: 1713 189 1275 488 5667
Fragments arranged by size:
5667 1713 1275 488 189
DraIII CAC_nnn'GTG
Cuts at: 1582 4358 1582
Size: 2776 6556
DrdI GACnn_nn'nnGTC
Cuts at: 7038 7896 7038
Size: 858 8474
EaeI y'GGCC_r
Cuts at: 110 1412 3023 4389 4862 4982 5415 6917 7091
Size: 1302 1611 1366 473 120 433 1502 174
Cuts at: 7091 7482 7509 8854 110
Size: 391 27 1345 588
Fragments arranged by size:
1611 1502 1366 1345 1302 588 473 433
391 174 120 27
EagI C'GGCC_G
Cuts at: 6917 6917
Size: 9332
EarI CTCTTCn'nnn_
Cuts at: 1059 1404 1443 1970 2737 3035 3491 4178 4524
Size: 345 39 527 767 298 456 687 346
Cuts at: 4524 5177 5336 5800 7355 7565 1059
Size: 653 159 464 1555 210 2826
Fragments arranged by size:
2826 1555 767 687 653 527 464 456
346 345 298 210 159 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2430 7540 7850 7996 9142 2430
Size: 5110 310 146 1146 2620
Fragments arranged by size:
5110 2620 1146 310 146
EcoICRI GAG'CTC
Cuts at: 165 2652 4035 4956 6344 165
Size: 2487 1383 921 1388 3153
Fragments arranged by size:
3153 2487 1388 1383 921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 3220 3472 4192 4286 4334 4475 4496 4900
Size: 869 252 720 94 48 141 21 404
Cuts at: 4900 5215 5382 6341 7157 7589 8336 8604 2351
Size: 315 167 959 816 432 747 268 3079
Fragments arranged by size:
3079 959 869 816 747 720 432 404
315 268 252 167 141 94 48 21
EcoNI CCTnn'n_nnAGG
Cuts at: 4671 4671
Size: 9332
EcoO109I rG'GnC_Cy
Cuts at: 392 5706 6471 392
Size: 5314 765 3253
Fragments arranged by size:
5314 3253 765
EcoRI G'AATT_C
Cuts at: 2113 6336 2113
Size: 4223 5109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1747 2880 3462 5646 7351 1747
Size: 1133 582 2184 1705 3728
Fragments arranged by size:
3728 2184 1705 1133 582
FspI TGC'GCA
Cuts at: 121 7113 121
Size: 6992 2340
HaeII r_GCGC'y
Cuts at: 3207 6766 7014 8036 9015 3207
Size: 3559 248 1022 979 3524
Fragments arranged by size:
3559 3524 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 327 359 513 545 556 559 588 591 1026
Size: 32 154 32 11 3 29 3 435
Cuts at: 1026 1058 1100 1132 2467 2499 2947 2979 3075
Size: 32 42 32 1335 32 448 32 96
Cuts at: 3075 3107 3244 3276 4138 4170 4327 4359 4481
Size: 32 137 32 862 32 157 32 122
Cuts at: 4481 4513 4665 4697 7174 7204 7206 7236 327
Size: 32 152 32 2477 30 2 30 2423
Fragments arranged by size:
2477 2423 1335 862 448 435 157 154
152 137 122 96 42 32 32 32
32 32 32 32 32 32 32 32
32 30 30 29 11 3 3 2
HincII GTy'rAC
Cuts at: 346 6371 346
Size: 6025 3307
HindIII A'AGCT_T
Cuts at: 1780 3055 5899 1780
Size: 1275 2844 5213
Fragments arranged by size:
5213 2844 1275
KasI G'GCGC_C
Cuts at: 6762 7010 6762
Size: 248 9084
KpnI G_GTAC'C
Cuts at: 2389 6352 2389
Size: 3963 5369
MfeI C'AATT_G
Cuts at: 1329 3601 5849 1329
Size: 2272 2248 4812
Fragments arranged by size:
4812 2272 2248
MluI A'CGCG_T
Cuts at: 8765 8765
Size: 9332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1127 2892 3017 3600 5961 8003 8187 1127
Size: 1765 125 583 2361 2042 184 2272
Fragments arranged by size:
2361 2272 2042 1765 583 184 125
MscI TGG'CCA
Cuts at: 1414 3025 4391 4864 4984 5417 7093 1414
Size: 1611 1366 473 120 433 1676 3653
Fragments arranged by size:
3653 1676 1611 1366 473 433 120
MslI CAynn'nnrTG
Cuts at: 2036 2749 2762 5177 7321 7448 2036
Size: 713 13 2415 2144 127 3920
Fragments arranged by size:
3920 2415 2144 713 127 13
MspA1I CmG'CkG
Cuts at: 2430 2616 2901 3390 6443 6757 7117 8130 8375
Size: 186 285 489 3053 314 360 1013 245
Cuts at: 8375 2430
Size: 3387
Fragments arranged by size:
3387 3053 1013 489 360 314 285 245
186
NaeI GCC'GGC
Cuts at: 7513 7513
Size: 9332
NarI GG'CG_CC
Cuts at: 6763 7011 6763
Size: 248 9084
NcoI C'CATG_G
Cuts at: 107 2681 2786 107
Size: 2574 105 6653
Fragments arranged by size:
6653 2574 105
NdeI CA'TA_TG
Cuts at: 1920 5991 1920
Size: 4071 5261
NgoMIV G'CCGG_C
Cuts at: 7511 7511
Size: 9332
NheI G'CTAG_C
Cuts at: 3190 6022 3190
Size: 2832 6500
NsiI A_TGCA'T
Cuts at: 1043 3555 5369 6233 1043
Size: 2512 1814 864 4142
Fragments arranged by size:
4142 2512 1814 864
NspI r_CATG'y
Cuts at: 1041 2590 2680 3553 4474 4542 6066 6089 6262
Size: 1549 90 873 921 68 1524 23 173
Cuts at: 6262 6385 6885 7792 1041
Size: 123 500 907 2581
Fragments arranged by size:
2581 1549 1524 921 907 873 500 173
123 90 68 23
PciI A'CATG_T
Cuts at: 4470 4538 6062 6258 7788 4470
Size: 68 1524 196 1530 6014
Fragments arranged by size:
6014 1530 1524 196 68
PflMI CCAn_nnn'nTGG
Cuts at: 861 861
Size: 9332
PfoI T'CCnGG_A
Cuts at: 2117 2117
Size: 9332
PmeI GTTT'AAAC
Cuts at: 6331 6331
Size: 9332
PmlI CAC'GTG
Cuts at: 447 447
Size: 9332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5456 5488 8471 8503 5456
Size: 32 2983 32 6285
Fragments arranged by size:
6285 2983 32 32
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 9332
PspOMI G'GGCC_C
Cuts at: 392 392
Size: 9332
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1868 1900 1868
Size: 32 9300
PstI C_TGCA'G
Cuts at: 1847 2175 2955 3389 5889 6379 7064 1847
Size: 328 780 434 2500 490 685 4115
Fragments arranged by size:
4115 2500 780 685 490 434 328
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9332
PvuII CAG'CTG
Cuts at: 2430 2616 2901 6757 7117 2430
Size: 186 285 3856 360 4645
Fragments arranged by size:
4645 3856 360 285 186
RsrII CG'GwC_CG
Cuts at: 7527 7527
Size: 9332
SacI G_AGCT'C
Cuts at: 167 2654 4037 4958 6346 167
Size: 2487 1383 921 1388 3153
Fragments arranged by size:
3153 2487 1388 1383 921
SalI G'TCGA_C
Cuts at: 6369 6369
Size: 9332
SapI GCTCTTCn'nnn_
Cuts at: 1404 1443 3491 4178 7355 7565 1404
Size: 39 2048 687 3177 210 3171
Fragments arranged by size:
3177 3171 2048 687 210 39
SbfI CC_TGCA'GG
Cuts at: 2175 6379 2175
Size: 4204 5128
ScaI AGT'ACT
Cuts at: 2986 4331 8849 2986
Size: 1345 4518 3469
Fragments arranged by size:
4518 3469 1345
SfcI C'TryA_G
Cuts at: 33 429 1561 1843 2171 2951 3180 3385 4684
Size: 396 1132 282 328 780 229 205 1299
Cuts at: 4684 5885 6375 7060 8053 8244 33
Size: 1201 490 685 993 191 1121
Fragments arranged by size:
1299 1201 1132 1121 993 780 685 490
396 328 282 229 205 191
SfoI GGC'GCC
Cuts at: 6764 7012 6764
Size: 248 9084
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9332
SmaI CCC'GGG
Cuts at: 6354 6354
Size: 9332
SmlI C'TyrA_G
Cuts at: 832 1721 1801 2576 5315 5496 6487 7402 7894
Size: 889 80 775 2739 181 991 915 492
Cuts at: 7894 8156 832
Size: 262 2008
Fragments arranged by size:
2739 2008 991 915 889 775 492 262
181 80
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9332
SphI G_CATG'C
Cuts at: 1041 2680 6089 6385 6885 1041
Size: 1639 3409 296 500 3488
Fragments arranged by size:
3488 3409 1639 500 296
SspI AAT'ATT
Cuts at: 1959 2002 2662 2939 1959
Size: 43 660 277 8352
Fragments arranged by size:
8352 660 277 43
StyI C'CwwG_G
Cuts at: 107 996 1665 2084 2681 2786 6466 6820 107
Size: 889 669 419 597 105 3680 354 2619
Fragments arranged by size:
3680 2619 889 669 597 419 354 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1290 4163 5677 6308 6915 7048 7507 7716 1290
Size: 2873 1514 631 607 133 459 209 2906
Fragments arranged by size:
2906 2873 1514 631 607 459 209 133
TatI w'GTAC_w
Cuts at: 2984 4329 5450 8847 2984
Size: 1345 1121 3397 3469
Fragments arranged by size:
3469 3397 1345 1121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8849 8881 8849
Size: 32 9300
Tth111I GACn'n_nGTC
Cuts at: 7129 7129
Size: 9332
XbaI T'CTAG_A
Cuts at: 6363 6363
Size: 9332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3033 6827 3033
Size: 3794 5538
XmaI C'CCGG_G
Cuts at: 6352 6352
Size: 9332
XmnI GAAnn'nnTTC
Cuts at: 521 667 1532 1796 1883 521
Size: 146 865 264 87 7970
Fragments arranged by size:
7970 865 264 146 87
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PciI PflMI PfoI PmeI
PmlI PpiI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BbvCI
BcgI BsiWI BsrGI BssHII BstEII BstXI ClaI EcoRV
FseI FspAI HpaI NotI NruI PacI PasI PpuMI
PshAI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI