Restriction map for
pF1KE2357
(Circular) (Six-base) MAPSORT of: pF1KE2357.seq Check: 6910 from: 1 to: 10058
pF1KE2357 10058 bp
With 182 enzymes: SgfI *
August 9, 2016 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4849 4849
Size: 10058
AccI GT'mk_AC
Cuts at: 1325 2339 2363 7096 1325
Size: 1014 24 4733 4287
Fragments arranged by size:
4733 4287 1014 24
Acc65I G'GTAC_C
Cuts at: 3954 4768 7074 3954
Size: 814 2306 6938
Fragments arranged by size:
6938 2306 814
AclI AA'CG_TT
Cuts at: 9898 9898
Size: 10058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 1400 1694 1850 1877 2571 2630 2750 2855
Size: 151 294 156 27 694 59 120 105
Cuts at: 2855 3662 4571 5282 5552 6587 7883 8315 9062
Size: 807 909 711 270 1035 1296 432 747
Cuts at: 9062 9330 1249
Size: 268 1977
Fragments arranged by size:
1977 1296 1035 909 807 747 711 694
432 294 270 268 156 151 120 105
59 27
AflII C'TTAA_G
Cuts at: 337 337
Size: 10058
AflIII A'CryG_T
Cuts at: 271 2151 3326 5772 7410 8514 9491 271
Size: 1880 1175 2446 1638 1104 977 838
Fragments arranged by size:
2446 1880 1638 1175 1104 977 838
AgeI A'CCGG_T
Cuts at: 2358 8446 2358
Size: 6088 3970
AhdI GACnn_n'nnGTC
Cuts at: 1114 5776 1114
Size: 4662 5396
AleI CACnn'nnGTG
Cuts at: 5906 5906
Size: 10058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3334 4058 5708 3334
Size: 724 1650 7684
Fragments arranged by size:
7684 1650 724
AlwNI CAG_nnn'CTG
Cuts at: 1984 2695 2878 6195 7363 8930 1984
Size: 711 183 3317 1168 1567 3112
Fragments arranged by size:
3317 3112 1567 1168 711 183
ApaLI G'TGCA_C
Cuts at: 2555 8828 2555
Size: 6273 3785
ApoI r'AATT_y
Cuts at: 349 550 675 1192 1570 1608 2779 3118 3184
Size: 201 125 517 378 38 1171 339 66
Cuts at: 3184 3272 3789 4112 4545 5266 5712 6379 6499
Size: 88 517 323 433 721 446 667 120
Cuts at: 6499 6846 7062 9411 9667 349
Size: 347 216 2349 256 740
Fragments arranged by size:
2349 1171 740 721 667 517 517 446
433 378 347 339 323 256 216 201
125 120 88 66 38
AseI AT'TA_AT
Cuts at: 20 419 1369 20
Size: 399 950 8709
Fragments arranged by size:
8709 950 399
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10058
AvaI C'yCGr_G
Cuts at: 1112 7078 1112
Size: 5966 4092
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3812 3845 4172 4205 4559 4592 6059 6092 3812
Size: 33 327 33 354 33 1467 33 7778
Fragments arranged by size:
7778 1467 354 327 33 33 33 33
BamHI G'GATC_C
Cuts at: 2040 4847 7083 2040
Size: 2807 2236 5015
Fragments arranged by size:
5015 2807 2236
BanI G'GyrC_C
Cuts at: 2667 3954 4768 7074 7488 7736 7771 2667
Size: 1287 814 2306 414 248 35 4954
Fragments arranged by size:
4954 2306 1287 814 414 248 35
BanII G_rGCy'C
Cuts at: 2720 2914 5749 7072 8102 2720
Size: 194 2835 1323 1030 4676
Fragments arranged by size:
4676 2835 1323 1030 194
BbeI G_GCGC'C
Cuts at: 7492 7740 7492
Size: 248 9810
BbsI GAAGACnn'nnnn_
Cuts at: 2176 2534 3020 5766 6048 2176
Size: 358 486 2746 282 6186
Fragments arranged by size:
6186 2746 486 358 282
BbvCI CC'TCA_GC
Cuts at: 3624 4991 6946 3624
Size: 1367 1955 6736
Fragments arranged by size:
6736 1955 1367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 5350 5384 90
Size: 34 5226 34 4764
Fragments arranged by size:
5226 4764 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 172 4587 7956 8717 10058 172
Size: 4415 3369 761 1341 172
Fragments arranged by size:
4415 3369 1341 761 172
BclI T'GATC_A
Cuts at: 3441 4197 5394 5619 6878 7578 3441
Size: 756 1197 225 1259 700 5921
Fragments arranged by size:
5921 1259 1197 756 700 225
BfrBI ATG'CAT
Cuts at: 2859 2859
Size: 10058
BglII A'GATC_T
Cuts at: 312 1747 2226 7573 9610 312
Size: 1435 479 5347 2037 760
Fragments arranged by size:
5347 2037 1435 760 479
BlpI GC'TnA_GC
Cuts at: 7170 7170
Size: 10058
Bme1580I G_kGCm'C
Cuts at: 2559 2672 3716 7683 7776 8832 2559
Size: 113 1044 3967 93 1056 3785
Fragments arranged by size:
3967 3785 1056 1044 113 93
BmgBI CAC'GTC
Cuts at: 5773 5773
Size: 10058
BmrI ACTGGGnnnn_n'
Cuts at: 410 504 2191 2504 3710 5759 7270 7351 7686
Size: 94 1687 313 1206 2049 1511 81 335
Cuts at: 7686 9830 410
Size: 2144 638
Fragments arranged by size:
2144 2049 1687 1511 1206 638 335 313
94 81
BmtI G_CTAG'C
Cuts at: 5416 5416
Size: 10058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8425 8425
Size: 10058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1857 1871 2113 2286 3119 5112 5562 5933 6447
Size: 14 242 173 833 1993 450 371 514
Cuts at: 6447 1857
Size: 5468
Fragments arranged by size:
5468 1993 833 514 450 371 242 173
14
Bpu10I CC'TnA_GC
Cuts at: 3624 4991 6196 6946 3624
Size: 1367 1205 750 6736
Fragments arranged by size:
6736 1367 1205 750
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 2810 3488 7234 8113 8605 8903 580
Size: 2230 678 3746 879 492 298 1735
Fragments arranged by size:
3746 2230 1735 879 678 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2325 3613 9762 2325
Size: 1288 6149 2621
Fragments arranged by size:
6149 2621 1288
BsaAI yAC'GTr
Cuts at: 2152 7411 2152
Size: 5259 4799
BsaBI GATnn'nnATC
Cuts at: 6 311 568 1208 7572 9609 6
Size: 305 257 640 6364 2037 455
Fragments arranged by size:
6364 2037 640 455 305 257
BsaHI Gr'CG_yC
Cuts at: 7489 7737 8439 9935 7489
Size: 248 702 1496 7612
Fragments arranged by size:
7612 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2358 7247 7768 8446 8720 8867 9278 2358
Size: 4889 521 678 274 147 411 3138
Fragments arranged by size:
4889 3138 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 804 834 5601 5631 804
Size: 30 4767 30 5231
Fragments arranged by size:
5231 4767 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 464 809 1411 1828 1831 5662 6058 6133
Size: 328 345 602 417 3 3831 396 75
Cuts at: 6133 6800 136
Size: 667 3394
Fragments arranged by size:
3831 3394 667 602 417 396 345 328
75 3
BseYI C'CCAG_C
Cuts at: 2695 2719 2839 6392 7483 8241 8818 9314 2695
Size: 24 120 3553 1091 758 577 496 3439
Fragments arranged by size:
3553 3439 1091 758 577 496 120 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 810 3799 5096 5320 7025 810
Size: 2989 1297 224 1705 3843
Fragments arranged by size:
3843 2989 1705 1297 224
BsiEI CG_ry'CG
Cuts at: 104 7600 7646 8854 104
Size: 7496 46 1208 1308
Fragments arranged by size:
7496 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 237 2559 4695 6813 6961 7072 7850 8040 8832
Size: 2322 2136 2118 148 111 778 190 792
Cuts at: 8832 237
Size: 1463
Fragments arranged by size:
2322 2136 2118 1463 792 778 190 148
111
BsmI GAATG_Cn'
Cuts at: 137 3357 137
Size: 3220 6838
BsmBI CGTCTCn'nnnn_
Cuts at: 2764 2764
Size: 10058
Bsp1286I G_dGCh'C
Cuts at: 237 2559 2672 2720 2914 3716 4695 5749 6813
Size: 2322 113 48 194 802 979 1054 1064
Cuts at: 6813 6961 7072 7683 7776 7850 8040 8102 8832
Size: 148 111 611 93 74 190 62 730
Cuts at: 8832 237
Size: 1463
Fragments arranged by size:
2322 1463 1064 1054 979 802 730 611
194 190 148 113 111 93 74 62
48
BspEI T'CCGG_A
Cuts at: 9278 9278
Size: 10058
BspHI T'CATG_A
Cuts at: 1026 3444 5579 6063 6771 9234 1026
Size: 2418 2135 484 708 2463 1850
Fragments arranged by size:
2463 2418 2135 1850 708 484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4849 7108 7322 7624 8005 4849
Size: 2259 214 302 381 6902
Fragments arranged by size:
6902 2259 381 302 214
BsrBI CCG'CTC
Cuts at: 50 8350 8404 9888 10053 50
Size: 8300 54 1484 165 55
Fragments arranged by size:
8300 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 6250 6381 7009 7970 6250
Size: 131 628 961 8338
Fragments arranged by size:
8338 961 628 131
BsrFI r'CCGG_y
Cuts at: 2358 4336 8056 8237 8446 2358
Size: 1978 3720 181 209 3970
Fragments arranged by size:
3970 3720 1978 209 181
BsrGI T'GTAC_A
Cuts at: 3965 3965
Size: 10058
BssSI C'ACGA_G
Cuts at: 5593 8329 8687 5593
Size: 2736 358 6964
Fragments arranged by size:
6964 2736 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1888 5422 5866 7435 1888
Size: 3534 444 1569 4511
Fragments arranged by size:
4511 3534 1569 444
BstBI TT'CG_AA
Cuts at: 8419 8419
Size: 10058
BstXI CCAn_nnnn'nTGG
Cuts at: 4258 6990 4258
Size: 2732 7326
BstYI r'GATC_y
Cuts at: 1 312 744 1747 2040 2226 2444 4847 7083
Size: 311 432 1003 293 186 218 2403 2236
Cuts at: 7083 7550 7573 7908 8154 9166 9252 9264 9610
Size: 467 23 335 246 1012 86 12 346
Cuts at: 9610 1
Size: 449
Fragments arranged by size:
2403 2236 1012 1003 467 449 432 346
335 311 293 246 218 186 86 23
12
BstZ17I GTA'TAC
Cuts at: 2340 2364 2340
Size: 24 10034
BtgI C'CryG_G
Cuts at: 2324 6851 2324
Size: 4527 5531
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 8008 8189 8352 8008
Size: 181 163 9714
Fragments arranged by size:
9714 181 163
BtsI GCAGTG_nn'
Cuts at: 2850 5806 5888 5901 5913 7301 2850
Size: 2956 82 13 12 1388 5607
Fragments arranged by size:
5607 2956 1388 82 13 12
ClaI AT'CG_AT
Cuts at: 5370 5370
Size: 10058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 5576 5611 5576
Size: 35 10023
DraI TTT'AAA
Cuts at: 471 855 1607 1721 7057 471
Size: 384 752 114 5336 3472
Fragments arranged by size:
5336 3472 752 384 114
DraIII CAC_nnn'GTG
Cuts at: 1698 3951 5801 1698
Size: 2253 1850 5955
Fragments arranged by size:
5955 2253 1850
DrdI GACnn_nn'nnGTC
Cuts at: 439 2178 7764 8622 439
Size: 1739 5586 858 1875
Fragments arranged by size:
5586 1875 1739 858
EaeI y'GGCC_r
Cuts at: 3992 6477 6511 6679 7643 7817 8208 8235 9580
Size: 2485 34 168 964 174 391 27 1345
Cuts at: 9580 3992
Size: 4470
Fragments arranged by size:
4470 2485 1345 964 391 174 168 34
27
EagI C'GGCC_G
Cuts at: 7643 7643
Size: 10058
EarI CTCTTCn'nnn_
Cuts at: 203 1013 1397 2387 3111 5671 6965 8081 8291
Size: 810 384 990 724 2560 1294 1116 210
Cuts at: 8291 203
Size: 1970
Fragments arranged by size:
2560 1970 1294 1116 990 810 724 384
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8266 8576 8722 9868 8266
Size: 310 146 1146 8456
Fragments arranged by size:
8456 1146 310 146
EcoICRI GAG'CTC
Cuts at: 7070 7070
Size: 10058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 1400 1694 1850 1857 1871 1877 2113 2286
Size: 151 294 156 7 14 6 236 173
Cuts at: 2286 2571 2630 2750 2855 3119 3662 4571 5112
Size: 285 59 120 105 264 543 909 541
Cuts at: 5112 5282 5552 5562 5933 6447 6587 7883 8315
Size: 170 270 10 371 514 140 1296 432
Cuts at: 8315 9062 9330 1249
Size: 747 268 1977
Fragments arranged by size:
1977 1296 909 747 543 541 514 432
371 294 285 270 268 264 236 173
170 156 151 140 120 105 59 14
10 7 6
EcoO109I rG'GnC_Cy
Cuts at: 2212 2875 3410 5887 7197 2212
Size: 663 535 2477 1310 5073
Fragments arranged by size:
5073 2477 1310 663 535
EcoRI G'AATT_C
Cuts at: 1570 4545 6379 7062 1570
Size: 2975 1834 683 4566
Fragments arranged by size:
4566 2975 1834 683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2688 8077 2688
Size: 5389 4669
FspI TGC'GCA
Cuts at: 7839 7839
Size: 10058
HaeII r_GCGC'y
Cuts at: 1430 3229 5877 7492 7740 8762 9741 1430
Size: 1799 2648 1615 248 1022 979 1747
Fragments arranged by size:
2648 1799 1747 1615 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 355 387 804 836 1100 1132 1900 1932 2092
Size: 32 417 32 264 32 768 32 160
Cuts at: 2092 2124 2305 2337 2731 2763 3544 3576 4402
Size: 32 181 32 394 32 781 32 826
Cuts at: 4402 4434 5122 5154 5762 5794 6867 6899 6964
Size: 32 688 32 608 32 1073 32 65
Cuts at: 6964 6996 7900 7930 7932 7962 355
Size: 32 904 30 2 30 2451
Fragments arranged by size:
2451 1073 904 826 781 768 688 608
417 394 264 181 160 65 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 840 1326 2331 3681 5316 5985 7097 840
Size: 486 1005 1350 1635 669 1112 3801
Fragments arranged by size:
3801 1635 1350 1112 1005 669 486
HindIII A'AGCT_T
Cuts at: 1840 4093 5492 1840
Size: 2253 1399 6406
Fragments arranged by size:
6406 2253 1399
KasI G'GCGC_C
Cuts at: 7488 7736 7488
Size: 248 9810
KpnI G_GTAC'C
Cuts at: 3958 4772 7078 3958
Size: 814 2306 6938
Fragments arranged by size:
6938 2306 814
MfeI C'AATT_G
Cuts at: 4061 4061
Size: 10058
MluI A'CGCG_T
Cuts at: 9491 9491
Size: 10058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 292 1145 2085 4189 4979 5175 5188 5366 5898
Size: 853 940 2104 790 196 13 178 532
Cuts at: 5898 8729 8913 292
Size: 2831 184 1437
Fragments arranged by size:
2831 2104 1437 940 853 790 532 196
184 178 13
MscI TGG'CCA
Cuts at: 6479 6513 6681 7819 6479
Size: 34 168 1138 8718
Fragments arranged by size:
8718 1138 168 34
MslI CAynn'nnrTG
Cuts at: 937 3316 4106 4256 4655 5906 6122 6128 6283
Size: 2379 790 150 399 1251 216 6 155
Cuts at: 6283 6559 8047 8174 937
Size: 276 1488 127 2821
Fragments arranged by size:
2821 2379 1488 1251 790 399 276 216
155 150 127 6
MspA1I CmG'CkG
Cuts at: 446 3709 4240 5530 7169 7483 7843 8856 9101
Size: 3263 531 1290 1639 314 360 1013 245
Cuts at: 9101 446
Size: 1403
Fragments arranged by size:
3263 1639 1403 1290 1013 531 360 314
245
NaeI GCC'GGC
Cuts at: 8239 8239
Size: 10058
NarI GG'CG_CC
Cuts at: 7489 7737 7489
Size: 248 9810
NdeI CA'TA_TG
Cuts at: 1279 1757 1962 3860 1279
Size: 478 205 1898 7477
Fragments arranged by size:
7477 1898 478 205
NgoMIV G'CCGG_C
Cuts at: 8237 8237
Size: 10058
NheI G'CTAG_C
Cuts at: 5412 5412
Size: 10058
NsiI A_TGCA'T
Cuts at: 2861 2861
Size: 10058
NspI r_CATG'y
Cuts at: 275 1332 2494 2680 2863 3330 3402 3813 4039
Size: 1057 1162 186 183 467 72 411 226
Cuts at: 4039 4228 5230 5245 7111 7611 8518 275
Size: 189 1002 15 1866 500 907 1815
Fragments arranged by size:
1866 1815 1162 1057 1002 907 500 467
411 226 189 186 183 72 15
PciI A'CATG_T
Cuts at: 271 3326 8514 271
Size: 3055 5188 1815
Fragments arranged by size:
5188 3055 1815
PflMI CCAn_nnn'nTGG
Cuts at: 1164 2695 3951 5883 6459 6486 1164
Size: 1531 1256 1932 576 27 4736
Fragments arranged by size:
4736 1932 1531 1256 576 27
PfoI T'CCnGG_A
Cuts at: 4878 6150 4878
Size: 1272 8786
PmeI GTTT'AAAC
Cuts at: 7057 7057
Size: 10058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9197 9229 9197
Size: 32 10026
PpuMI rG'GwC_Cy
Cuts at: 2212 3410 2212
Size: 1198 8860
PshAI GACnn'nnGTC
Cuts at: 4607 4607
Size: 10058
PsiI TTA'TAA
Cuts at: 2807 6604 6721 6730 2807
Size: 3797 117 9 6135
Fragments arranged by size:
6135 3797 117 9
PspXI vC'TCGA_Gb
Cuts at: 1112 1112
Size: 10058
PstI C_TGCA'G
Cuts at: 1779 2193 3062 5908 6234 7105 7790 1779
Size: 414 869 2846 326 871 685 4047
Fragments arranged by size:
4047 2846 871 869 685 414 326
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10058
PvuII CAG'CTG
Cuts at: 3709 4240 5530 7483 7843 3709
Size: 531 1290 1953 360 5924
Fragments arranged by size:
5924 1953 1290 531 360
RsrII CG'GwC_CG
Cuts at: 8253 8253
Size: 10058
SacI G_AGCT'C
Cuts at: 7072 7072
Size: 10058
SalI G'TCGA_C
Cuts at: 1324 7095 1324
Size: 5771 4287
SanDI GG'GwC_CC
Cuts at: 2212 3410 2212
Size: 1198 8860
SapI GCTCTTCn'nnn_
Cuts at: 8081 8291 8081
Size: 210 9848
SbfI CC_TGCA'GG
Cuts at: 7105 7105
Size: 10058
ScaI AGT'ACT
Cuts at: 3639 5626 5827 9575 3639
Size: 1987 201 3748 4122
Fragments arranged by size:
4122 3748 1987 201
SexAI A'CCwGG_T
Cuts at: 6863 6863
Size: 10058
SfcI C'TryA_G
Cuts at: 33 183 1775 2078 2189 2394 3058 3176 4211
Size: 150 1592 303 111 205 664 118 1035
Cuts at: 4211 4449 5904 6230 7101 7786 8779 8970 33
Size: 238 1455 326 871 685 993 191 1121
Fragments arranged by size:
1592 1455 1121 1035 993 871 685 664
326 303 238 205 191 150 118 111
SfoI GGC'GCC
Cuts at: 7490 7738 7490
Size: 248 9810
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10058
SmaI CCC'GGG
Cuts at: 7080 7080
Size: 10058
SmlI C'TyrA_G
Cuts at: 337 559 1112 2825 3503 7213 8128 8620 8882
Size: 222 553 1713 678 3710 915 492 262
Cuts at: 8882 337
Size: 1513
Fragments arranged by size:
3710 1713 1513 915 678 553 492 262
222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10058
SphI G_CATG'C
Cuts at: 2494 2680 2863 7111 7611 2494
Size: 186 183 4248 500 4941
Fragments arranged by size:
4941 4248 500 186 183
SspI AAT'ATT
Cuts at: 303 3728 303
Size: 3425 6633
StyI C'CwwG_G
Cuts at: 1920 3392 5508 7192 7546 1920
Size: 1472 2116 1684 354 4432
Fragments arranged by size:
4432 2116 1684 1472 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 605 1210 2853 2959 4336 4504 5363 7641 7774
Size: 605 1643 106 1377 168 859 2278 133
Cuts at: 7774 8233 8442 605
Size: 459 209 2221
Fragments arranged by size:
2278 2221 1643 1377 859 605 459 209
168 133 106
TatI w'GTAC_w
Cuts at: 974 3637 3965 5134 5308 5624 5825 6645 9573
Size: 2663 328 1169 174 316 201 820 2928
Cuts at: 9573 974
Size: 1459
Fragments arranged by size:
2928 2663 1459 1169 820 328 316 201
174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 9575 9607 5601
Size: 32 3942 32 6052
Fragments arranged by size:
6052 3942 32 32
Tth111I GACn'n_nGTC
Cuts at: 7855 7855
Size: 10058
XbaI T'CTAG_A
Cuts at: 985 7089 985
Size: 6104 3954
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1165 2331 5772 5908 7553 1165
Size: 1166 3441 136 1645 3670
Fragments arranged by size:
3670 3441 1645 1166 136
XhoI C'TCGA_G
Cuts at: 1112 1112
Size: 10058
XmaI C'CCGG_G
Cuts at: 7078 7078
Size: 10058
XmnI GAAnn'nnTTC
Cuts at: 1022 1058 1995 2408 2600 5101 6184 6270 1022
Size: 36 937 413 192 2501 1083 86 4810
Fragments arranged by size:
4810 2501 1083 937 413 192 86 36
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NheI NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AloI ApaI AscI AvrII BglI BsiWI
BssHII BstEII Bsu36I EcoNI EcoRV FseI FspAI HpaI
NcoI NotI NruI PacI PasI PmlI PspOMI PsrI
SacII SfiI SgrAI SnaBI SrfI StuI SwaI ZraI