Restriction map for
pF1KE2346
(Circular) (Six-base) MAPSORT of: pF1KE2346.seq Check: 8342 from: 1 to: 11525
pF1KE2346 11525 bp
With 182 enzymes: SgfI *
November 15, 2011 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 733 733
Size: 11525
AatII G_ACGT'C
Cuts at: 8228 8228
Size: 11525
AccI GT'mk_AC
Cuts at: 2891 5513 5631 8396 8563 2891
Size: 2622 118 2765 167 5853
Fragments arranged by size:
5853 2765 2622 167 118
Acc65I G'GTAC_C
Cuts at: 8541 8541
Size: 11525
AclI AA'CG_TT
Cuts at: 11365 11365
Size: 11525
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1193 1415 1829 1834 2459 2522 3698 3995 4327
Size: 222 414 5 625 63 1176 297 332
Cuts at: 4327 4509 5231 8135 8295 9350 9782 10529 10797
Size: 182 722 2904 160 1055 432 747 268
Cuts at: 10797 1193
Size: 1921
Fragments arranged by size:
2904 1921 1176 1055 747 722 625 432
414 332 297 268 222 182 160 63
5
AfeI AGC'GCT
Cuts at: 1576 7275 1576
Size: 5699 5826
AflIII A'CryG_T
Cuts at: 3309 6844 7623 8877 9981 10958 3309
Size: 3535 779 1254 1104 977 3876
Fragments arranged by size:
3876 3535 1254 1104 977 779
AgeI A'CCGG_T
Cuts at: 9913 9913
Size: 11525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 8401 8401
Size: 11525
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6918 6950 6918
Size: 32 11493
AlwNI CAG_nnn'CTG
Cuts at: 1438 3568 3798 4849 7102 7183 7486 8830 10397
Size: 2130 230 1051 2253 81 303 1344 1567
Cuts at: 10397 1438
Size: 2566
Fragments arranged by size:
2566 2253 2130 1567 1344 1051 303 230
81
ApaLI G'TGCA_C
Cuts at: 859 10295 859
Size: 9436 2089
ApoI r'AATT_y
Cuts at: 498 2926 2970 3785 4129 4472 4797 5128 5894
Size: 2428 44 815 344 343 325 331 766
Cuts at: 5894 6099 6411 6418 7665 7692 7711 7796 8178
Size: 205 312 7 1247 27 19 85 382
Cuts at: 8178 8529 10878 11134 498
Size: 351 2349 256 889
Fragments arranged by size:
2428 2349 1247 889 815 766 382 351
344 343 331 325 312 256 205 85
44 27 19 7
AseI AT'TA_AT
Cuts at: 20 1723 20
Size: 1703 9822
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11525
AvaI C'yCGr_G
Cuts at: 1028 8545 1028
Size: 7517 4008
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6435 6468 6435
Size: 33 11492
BamHI G'GATC_C
Cuts at: 8550 8550
Size: 11525
BanI G'GyrC_C
Cuts at: 6451 8385 8541 8955 9203 9238 6451
Size: 1934 156 414 248 35 8738
Fragments arranged by size:
8738 1934 414 248 156 35
BanII G_rGCy'C
Cuts at: 8539 9569 8539
Size: 1030 10495
BbeI G_GCGC'C
Cuts at: 8959 9207 8959
Size: 248 11277
BbsI GAAGACnn'nnnn_
Cuts at: 1199 2637 6899 7661 1199
Size: 1438 4262 762 5063
Fragments arranged by size:
5063 4262 1438 762
BbvCI CC'TCA_GC
Cuts at: 5636 5636
Size: 11525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5899 5933 5899
Size: 34 11491
BciVI GTATCCnnnnn_n'
Cuts at: 655 5676 9423 10184 11525 655
Size: 5021 3747 761 1341 655
Fragments arranged by size:
5021 3747 1341 761 655
BclI T'GATC_A
Cuts at: 7122 9045 7122
Size: 1923 9602
BfrBI ATG'CAT
Cuts at: 1525 5259 7458 1525
Size: 3734 2199 5592
Fragments arranged by size:
5592 3734 2199
BglI GCCn_nnn'nGGC
Cuts at: 406 4839 8372 406
Size: 4433 3533 3559
Fragments arranged by size:
4433 3559 3533
BglII A'GATC_T
Cuts at: 925 2818 3087 6321 9040 11077 925
Size: 1893 269 3234 2719 2037 1373
Fragments arranged by size:
3234 2719 2037 1893 1373 269
BlpI GC'TnA_GC
Cuts at: 3104 8360 8637 3104
Size: 5256 277 5992
Fragments arranged by size:
5992 5256 277
Bme1580I G_kGCm'C
Cuts at: 863 7090 9150 9243 10299 863
Size: 6227 2060 93 1056 2089
Fragments arranged by size:
6227 2089 2060 1056 93
BmgBI CAC'GTC
Cuts at: 2554 7380 2554
Size: 4826 6699
BmrI ACTGGGnnnn_n'
Cuts at: 619 5136 5513 6981 8737 8818 9153 11297 619
Size: 4517 377 1468 1756 81 335 2144 847
Fragments arranged by size:
4517 2144 1756 1468 847 377 335 81
BmtI G_CTAG'C
Cuts at: 8033 8033
Size: 11525
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1596 4653 6009 9892 1596
Size: 3057 1356 3883 3229
Fragments arranged by size:
3883 3229 3057 1356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 1046 6802 546
Size: 500 5756 5269
Fragments arranged by size:
5756 5269 500
Bpu10I CC'TnA_GC
Cuts at: 1925 5636 1925
Size: 3711 7814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 942 963 2721 4419 6988 8701 9580 10072 10370
Size: 21 1758 1698 2569 1713 879 492 298
Cuts at: 10370 942
Size: 2097
Fragments arranged by size:
2569 2097 1758 1713 1698 879 492 298
21
BsaI GGTCTCn'nnnn_
Cuts at: 2035 2511 3992 11229 2035
Size: 476 1481 7237 2331
Fragments arranged by size:
7237 2331 1481 476
BsaAI yAC'GTr
Cuts at: 6847 8878 6847
Size: 2031 9494
BsaBI GATnn'nnATC
Cuts at: 6 1742 2267 6575 9039 11076 6
Size: 1736 525 4308 2464 2037 455
Fragments arranged by size:
4308 2464 2037 1736 525 455
BsaHI Gr'CG_yC
Cuts at: 8225 8956 9204 9906 11402 8225
Size: 731 248 702 1496 8348
Fragments arranged by size:
8348 1496 731 702 248
BsaWI w'CCGG_w
Cuts at: 8714 9235 9913 10187 10334 10745 8714
Size: 521 678 274 147 411 9494
Fragments arranged by size:
9494 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3516 3546 4717 4747 5374 5404 5844 5874 6377
Size: 30 1171 30 627 30 440 30 503
Cuts at: 6377 6407 7279 7309 8329 8359 3516
Size: 30 872 30 1020 30 6682
Fragments arranged by size:
6682 1171 1020 872 627 503 440 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3578 3581 4208 4524 6771 8087 8153 8156 3578
Size: 3 627 316 2247 1316 66 3 6947
Fragments arranged by size:
6947 2247 1316 627 316 66 3 3
BseYI C'CCAG_C
Cuts at: 1648 3595 6158 8950 9708 10285 10781 1648
Size: 1947 2563 2792 758 577 496 2392
Fragments arranged by size:
2792 2563 2392 1947 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1309 4943 8311 1309
Size: 3634 3368 4523
Fragments arranged by size:
4523 3634 3368
BsiEI CG_ry'CG
Cuts at: 104 9067 9113 10321 104
Size: 8963 46 1208 1308
Fragments arranged by size:
8963 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 863 2107 6050 6555 8539 9317 9507 10299 863
Size: 1244 3943 505 1984 778 190 792 2089
Fragments arranged by size:
3943 2089 1984 1244 792 778 505 190
BsmI GAATG_Cn'
Cuts at: 909 1387 2092 909
Size: 478 705 10342
Fragments arranged by size:
10342 705 478
BsmBI CGTCTCn'nnnn_
Cuts at: 1681 1681
Size: 11525
Bsp1286I G_dGCh'C
Cuts at: 863 2107 6050 6555 7090 8539 9150 9243 9317
Size: 1244 3943 505 535 1449 611 93 74
Cuts at: 9317 9507 9569 10299 863
Size: 190 62 730 2089
Fragments arranged by size:
3943 2089 1449 1244 730 611 535 505
190 93 74 62
BspEI T'CCGG_A
Cuts at: 10745 10745
Size: 11525
BspHI T'CATG_A
Cuts at: 3510 10701 3510
Size: 7191 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 213 733 2942 7110 8575 8789 9091 9472 213
Size: 520 2209 4168 1465 214 302 381 2266
Fragments arranged by size:
4168 2266 2209 1465 520 381 302 214
BsrBI CCG'CTC
Cuts at: 50 9817 9871 11355 11520 50
Size: 9767 54 1484 165 55
Fragments arranged by size:
9767 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1142 1263 3015 3863 5068 5186 5888 6185 6249
Size: 121 1752 848 1205 118 702 297 64
Cuts at: 6249 7019 9437 1142
Size: 770 2418 3230
Fragments arranged by size:
3230 2418 1752 1205 848 770 702 297
121 118 64
BsrFI r'CCGG_y
Cuts at: 566 2234 9523 9704 9913 566
Size: 1668 7289 181 209 2178
Fragments arranged by size:
7289 2178 1668 209 181
BsrGI T'GTAC_A
Cuts at: 456 4088 456
Size: 3632 7893
BssSI C'ACGA_G
Cuts at: 1196 2786 9796 10154 1196
Size: 1590 7010 358 2567
Fragments arranged by size:
7010 2567 1590 358
BstAPI GCAn_nnn'nTGC
Cuts at: 8902 8902
Size: 11525
BstBI TT'CG_AA
Cuts at: 2628 5472 9886 2628
Size: 2844 4414 4267
Fragments arranged by size:
4414 4267 2844
BstYI r'GATC_y
Cuts at: 1 925 2647 2818 3087 4182 5002 5435 6321
Size: 924 1722 171 269 1095 820 433 886
Cuts at: 6321 6532 7236 8550 9017 9040 9375 9621 10633
Size: 211 704 1314 467 23 335 246 1012
Cuts at: 10633 10719 10731 11077 1
Size: 86 12 346 449
Fragments arranged by size:
1722 1314 1095 1012 924 886 820 704
467 449 433 346 335 269 246 211
171 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3791 6921 3791
Size: 3130 8395
BtgI C'CryG_G
Cuts at: 7305 7305
Size: 11525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 8223 9475 9656 9819 8223
Size: 1252 181 163 9929
Fragments arranged by size:
9929 1252 181 163
BtsI GCAGTG_nn'
Cuts at: 366 3840 4421 7184 8062 8310 8768 366
Size: 3474 581 2763 878 248 458 3123
Fragments arranged by size:
3474 3123 2763 878 581 458 248
DraI TTT'AAA
Cuts at: 2838 5021 5900 6417 6591 6794 8524 2838
Size: 2183 879 517 174 203 1730 5839
Fragments arranged by size:
5839 2183 1730 879 517 203 174
DraIII CAC_nnn'GTG
Cuts at: 858 3304 858
Size: 2446 9079
DrdI GACnn_nn'nnGTC
Cuts at: 385 2780 9231 10089 385
Size: 2395 6451 858 1821
Fragments arranged by size:
6451 2395 1821 858
EaeI y'GGCC_r
Cuts at: 3501 4840 5247 8373 9110 9284 9675 9702 11047
Size: 1339 407 3126 737 174 391 27 1345
Cuts at: 11047 3501
Size: 3979
Fragments arranged by size:
3979 3126 1345 1339 737 407 391 174
27
EagI C'GGCC_G
Cuts at: 9110 9110
Size: 11525
EarI CTCTTCn'nnn_
Cuts at: 194 245 1749 3455 3699 5798 5978 7433 7925
Size: 51 1504 1706 244 2099 180 1455 492
Cuts at: 7925 9548 9758 194
Size: 1623 210 1961
Fragments arranged by size:
2099 1961 1706 1623 1504 1455 492 244
210 180 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 9733 10043 10189 11335 9733
Size: 310 146 1146 9923
Fragments arranged by size:
9923 1146 310 146
EcoICRI GAG'CTC
Cuts at: 8537 8537
Size: 11525
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 1046 1193 1415 1829 1834 2459 2522 3698
Size: 500 147 222 414 5 625 63 1176
Cuts at: 3698 3995 4327 4509 5231 6802 8135 8295 9350
Size: 297 332 182 722 1571 1333 160 1055
Cuts at: 9350 9782 10529 10797 546
Size: 432 747 268 1274
Fragments arranged by size:
1571 1333 1274 1176 1055 747 722 625
500 432 414 332 297 268 222 182
160 147 63 5
EcoNI CCTnn'n_nnAGG
Cuts at: 316 3604 4190 5394 316
Size: 3288 586 1204 6447
Fragments arranged by size:
6447 3288 1204 586
EcoO109I rG'GnC_Cy
Cuts at: 1495 6486 8664 1495
Size: 4991 2178 4356
Fragments arranged by size:
4991 4356 2178
EcoRI G'AATT_C
Cuts at: 498 6099 8529 498
Size: 5601 2430 3494
Fragments arranged by size:
5601 3494 2430
EcoRV GAT'ATC
Cuts at: 1047 1484 1047
Size: 437 11088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 370 1004 2768 4831 5089 6314 9544 370
Size: 634 1764 2063 258 1225 3230 2351
Fragments arranged by size:
3230 2351 2063 1764 1225 634 258
FspI TGC'GCA
Cuts at: 9306 9306
Size: 11525
HaeII r_GCGC'y
Cuts at: 1578 7277 8367 8959 9207 10229 11208 1578
Size: 5699 1090 592 248 1022 979 1895
Fragments arranged by size:
5699 1895 1090 1022 979 592 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 144 176 462 494 1540 1572 1777 1809 1993
Size: 32 286 32 1046 32 205 32 184
Cuts at: 1993 2025 2452 2484 2515 2547 3160 3192 4987
Size: 32 427 32 31 32 613 32 1795
Cuts at: 4987 5019 5188 5220 6146 6178 6270 6302 6763
Size: 32 169 32 926 32 92 32 461
Cuts at: 6763 6795 6904 6936 7020 7052 7252 7284 7534
Size: 32 109 32 84 32 200 32 250
Cuts at: 7534 7566 9367 9397 9399 9429 144
Size: 32 1801 30 2 30 2240
Fragments arranged by size:
2240 1801 1795 1046 926 613 461 427
286 250 205 200 184 169 109 92
84 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 31 30 30 2
HincII GTy'rAC
Cuts at: 4907 5632 6475 6609 8223 8564 4907
Size: 725 843 134 1614 341 7868
Fragments arranged by size:
7868 1614 843 725 341 134
HindIII A'AGCT_T
Cuts at: 3401 3446 3874 3940 6014 3401
Size: 45 428 66 2074 8912
Fragments arranged by size:
8912 2074 428 66 45
HpaI GTT'AAC
Cuts at: 6609 6609
Size: 11525
KasI G'GCGC_C
Cuts at: 8955 9203 8955
Size: 248 11277
KpnI G_GTAC'C
Cuts at: 8545 8545
Size: 11525
MfeI C'AATT_G
Cuts at: 5462 5462
Size: 11525
MluI A'CGCG_T
Cuts at: 10958 10958
Size: 11525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1845 5076 5881 6528 10196 10380 1845
Size: 3231 805 647 3668 184 2990
Fragments arranged by size:
3668 3231 2990 805 647 184
MscI TGG'CCA
Cuts at: 3503 4842 5249 9286 3503
Size: 1339 407 4037 5742
Fragments arranged by size:
5742 4037 1339 407
MslI CAynn'nnrTG
Cuts at: 2390 2720 3509 5767 6290 6659 7168 9514 9641
Size: 330 789 2258 523 369 509 2346 127
Cuts at: 9641 2390
Size: 4274
Fragments arranged by size:
4274 2346 2258 789 523 509 369 330
127
MspA1I CmG'CkG
Cuts at: 183 4030 4846 8636 8950 9310 10323 10568 183
Size: 3847 816 3790 314 360 1013 245 1140
Fragments arranged by size:
3847 3790 1140 1013 816 360 314 245
NaeI GCC'GGC
Cuts at: 2236 9706 2236
Size: 7470 4055
NarI GG'CG_CC
Cuts at: 8956 9204 8956
Size: 248 11277
NdeI CA'TA_TG
Cuts at: 3657 7169 3657
Size: 3512 8013
NgoMIV G'CCGG_C
Cuts at: 2234 9704 2234
Size: 7470 4055
NheI G'CTAG_C
Cuts at: 8029 8029
Size: 11525
NsiI A_TGCA'T
Cuts at: 1527 5261 7460 1527
Size: 3734 2199 5592
Fragments arranged by size:
5592 3734 2199
NspI r_CATG'y
Cuts at: 444 669 4077 5927 6178 7229 8578 9078 9985
Size: 225 3408 1850 251 1051 1349 500 907
Cuts at: 9985 444
Size: 1984
Fragments arranged by size:
3408 1984 1850 1349 1051 907 500 251
225
PasI CC'CwG_GG
Cuts at: 7728 7728
Size: 11525
PciI A'CATG_T
Cuts at: 9981 9981
Size: 11525
PflMI CCAn_nnn'nTGG
Cuts at: 2755 6165 2755
Size: 3410 8115
PfoI T'CCnGG_A
Cuts at: 8347 8347
Size: 11525
PmeI GTTT'AAAC
Cuts at: 8524 8524
Size: 11525
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10664 10696 10664
Size: 32 11493
PpuMI rG'GwC_Cy
Cuts at: 1495 6486 1495
Size: 4991 6534
PshAI GACnn'nnGTC
Cuts at: 5584 5584
Size: 11525
PsiI TTA'TAA
Cuts at: 4268 7453 4268
Size: 3185 8340
PstI C_TGCA'G
Cuts at: 660 4428 7505 8572 9257 660
Size: 3768 3077 1067 685 2928
Fragments arranged by size:
3768 3077 2928 1067 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11525
PvuII CAG'CTG
Cuts at: 183 4846 8950 9310 183
Size: 4663 4104 360 2398
Fragments arranged by size:
4663 4104 2398 360
RsrII CG'GwC_CG
Cuts at: 9720 9720
Size: 11525
SacI G_AGCT'C
Cuts at: 8539 8539
Size: 11525
SalI G'TCGA_C
Cuts at: 5630 8562 5630
Size: 2932 8593
SapI GCTCTTCn'nnn_
Cuts at: 3455 7433 9548 9758 3455
Size: 3978 2115 210 5222
Fragments arranged by size:
5222 3978 2115 210
SbfI CC_TGCA'GG
Cuts at: 660 8572 660
Size: 7912 3613
ScaI AGT'ACT
Cuts at: 6626 7807 11042 6626
Size: 1181 3235 7109
Fragments arranged by size:
7109 3235 1181
SexAI A'CCwGG_T
Cuts at: 317 7202 317
Size: 6885 4640
SfcI C'TryA_G
Cuts at: 33 656 1658 2423 3110 4396 4424 5006 5510
Size: 623 1002 765 687 1286 28 582 504
Cuts at: 5510 6356 7501 8337 8568 9253 10246 10437 33
Size: 846 1145 836 231 685 993 191 1121
Fragments arranged by size:
1286 1145 1121 1002 993 846 836 765
687 685 623 582 504 231 191 28
SfoI GGC'GCC
Cuts at: 8957 9205 8957
Size: 248 11277
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11525
SmaI CCC'GGG
Cuts at: 8547 8547
Size: 11525
SmlI C'TyrA_G
Cuts at: 921 978 1028 2736 4434 6967 8680 9595 10087
Size: 57 50 1708 1698 2533 1713 915 492
Cuts at: 10087 10349 921
Size: 262 2097
Fragments arranged by size:
2533 2097 1713 1708 1698 915 492 262
57 50
SpeI A'CTAG_T
Cuts at: 67 2848 5112 5581 67
Size: 2781 2264 469 6011
Fragments arranged by size:
6011 2781 2264 469
SphI G_CATG'C
Cuts at: 444 5927 8578 9078 444
Size: 5483 2651 500 2891
Fragments arranged by size:
5483 2891 2651 500
SspI AAT'ATT
Cuts at: 3732 3924 5678 6762 3732
Size: 192 1754 1084 8495
Fragments arranged by size:
8495 1754 1084 192
StuI AGG'CCT
Cuts at: 2205 2304 7605 2205
Size: 99 5301 6125
Fragments arranged by size:
6125 5301 99
StyI C'CwwG_G
Cuts at: 354 1163 4944 8389 8501 8659 9013 354
Size: 809 3781 3445 112 158 354 2866
Fragments arranged by size:
3781 3445 2866 809 354 158 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1332 6898 7000 9108 9241 9700 9909 1332
Size: 5566 102 2108 133 459 209 2948
Fragments arranged by size:
5566 2948 2108 459 209 133 102
TatI w'GTAC_w
Cuts at: 456 4088 4121 6019 6624 6669 7805 8258 11040
Size: 3632 33 1898 605 45 1136 453 2782
Cuts at: 11040 456
Size: 941
Fragments arranged by size:
3632 2782 1898 1136 941 605 453 45
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 287 319 684 716 977 1009 3516 3548 7279
Size: 32 365 32 261 32 2507 32 3731
Cuts at: 7279 7311 7877 7909 11042 11074 287
Size: 32 566 32 3133 32 738
Fragments arranged by size:
3731 3133 2507 738 566 365 261 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 9322 9322
Size: 11525
XbaI T'CTAG_A
Cuts at: 7962 8556 7962
Size: 594 10931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 9020 9020
Size: 11525
XhoI C'TCGA_G
Cuts at: 1028 1028
Size: 11525
XmaI C'CCGG_G
Cuts at: 8545 8545
Size: 11525
XmnI GAAnn'nnTTC
Cuts at: 3525 6299 3525
Size: 2774 8751
ZraI GAC'GTC
Cuts at: 8226 8226
Size: 11525
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AleI ApaI AscI AvrII BsiWI BssHII
BstEII BstXI BstZ17I ClaI CspCI FseI FspAI NcoI
NotI NruI PacI PmlI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI