Restriction map for
pF1KE2345
(Circular) (Six-base) MAPSORT of: pF1KE2345.seq Check: 5551 from: 1 to: 4190
pF1KE2345 4190 bp
With 182 enzymes: SgfI *
May 20, 2011 15:42 ..
AccI GT'mk_AC
Cuts at: 1228 1228
Size: 4190
Acc65I G'GTAC_C
Cuts at: 1016 1151 1206 1016
Size: 135 55 4000
Fragments arranged by size:
4000 135 55
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1080 2015 2447 3194 3462 1080
Size: 935 432 747 268 1808
Fragments arranged by size:
1808 935 747 432 268
AflIII A'CryG_T
Cuts at: 764 1542 2646 3623 764
Size: 778 1104 977 1331
Fragments arranged by size:
1331 1104 977 778
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 180 741 1029 1495 3062 180
Size: 561 288 466 1567 1308
Fragments arranged by size:
1567 1308 561 466 288
ApaI G_GGCC'C
Cuts at: 124 931 124
Size: 807 3383
ApaLI G'TGCA_C
Cuts at: 427 2960 427
Size: 2533 1657
ApoI r'AATT_y
Cuts at: 1194 3543 3799 1194
Size: 2349 256 1585
Fragments arranged by size:
2349 1585 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 477 1210 477
Size: 733 3457
BamHI G'GATC_C
Cuts at: 111 1215 111
Size: 1104 3086
BanI G'GyrC_C
Cuts at: 357 1016 1151 1206 1620 1868 1903 357
Size: 659 135 55 414 248 35 2644
Fragments arranged by size:
2644 659 414 248 135 55 35
BanII G_rGCy'C
Cuts at: 124 931 1000 1204 2234 124
Size: 807 69 204 1030 2080
Fragments arranged by size:
2080 1030 807 204 69
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BciVI GTATCCnnnnn_n'
Cuts at: 413 550 2088 2849 4190 413
Size: 137 1538 761 1341 413
Fragments arranged by size:
1538 1341 761 413 137
BclI T'GATC_A
Cuts at: 1710 1710
Size: 4190
BglI GCCn_nnn'nGGC
Cuts at: 901 901
Size: 4190
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 124 431 462 917 931 1107 1815 1908 2964
Size: 307 31 455 14 176 708 93 1056
Cuts at: 2964 124
Size: 1350
Fragments arranged by size:
1350 1056 708 455 307 176 93 31
14
BmgBI CAC'GTC
Cuts at: 311 765 311
Size: 454 3736
BmrI ACTGGGnnnn_n'
Cuts at: 449 711 1402 1483 1818 3962 449
Size: 262 691 81 335 2144 677
Fragments arranged by size:
2144 691 677 335 262 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 919 640
Size: 279 3911
Bpu10I CC'TnA_GC
Cuts at: 1054 1054
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 739 1366 2245 2737 3035 203
Size: 536 627 879 492 298 1358
Fragments arranged by size:
1358 879 627 536 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 981 1543 981
Size: 562 3628
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 968 1621 1869 2571 4067 968
Size: 653 248 702 1496 1091
Fragments arranged by size:
1496 1091 702 653 248
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2852 2999 3410 1379
Size: 521 678 274 147 411 2159
Fragments arranged by size:
2159 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 613 643 760 790 802 832 613
Size: 30 117 30 12 30 3971
Fragments arranged by size:
3971 117 30 30 30 12
BseYI C'CCAG_C
Cuts at: 461 924 931 1100 1615 2373 2950 3446 461
Size: 463 7 169 515 758 577 496 1205
Fragments arranged by size:
1205 758 577 515 496 463 169 7
BsiEI CG_ry'CG
Cuts at: 104 393 1732 1778 2986 104
Size: 289 1339 46 1208 1308
Fragments arranged by size:
1339 1308 1208 289 46
BsiHKAI G_wGCw'C
Cuts at: 431 1204 1982 2172 2964 431
Size: 773 778 190 792 1657
Fragments arranged by size:
1657 792 778 773 190
BsmBI CGTCTCn'nnnn_
Cuts at: 805 1069 805
Size: 264 3926
Bsp1286I G_dGCh'C
Cuts at: 124 431 462 917 931 1000 1107 1204 1815
Size: 307 31 455 14 69 107 97 611
Cuts at: 1815 1908 1982 2172 2234 2964 124
Size: 93 74 190 62 730 1350
Fragments arranged by size:
1350 730 611 455 307 190 107 97
93 74 69 62 31 14
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 3366 3366
Size: 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1028 1240 1454 1756 2137 1028
Size: 212 214 302 381 3081
Fragments arranged by size:
3081 381 302 214 212
BsrBI CCG'CTC
Cuts at: 50 654 2482 2536 4020 4185 50
Size: 604 1828 54 1484 165 55
Fragments arranged by size:
1828 1484 604 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2102 2102
Size: 4190
BsrFI r'CCGG_y
Cuts at: 2188 2369 2578 2188
Size: 181 209 3800
Fragments arranged by size:
3800 209 181
BsrGI T'GTAC_A
Cuts at: 446 446
Size: 4190
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 1124 1567 1124
Size: 443 3747
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 1008 1008
Size: 4190
BstYI r'GATC_y
Cuts at: 1 111 500 1215 1682 1705 2040 2286 3298
Size: 110 389 715 467 23 335 246 1012
Cuts at: 3298 3384 3396 3742 1
Size: 86 12 346 449
Fragments arranged by size:
1012 715 467 449 389 346 335 246
110 86 23 12
BtgI C'CryG_G
Cuts at: 107 548 625 917 107
Size: 441 77 292 3380
Fragments arranged by size:
3380 441 292 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 510 2140 2321 2484 510
Size: 1630 181 163 2216
Fragments arranged by size:
2216 1630 181 163
BtsI GCAGTG_nn'
Cuts at: 383 528 698 1433 383
Size: 145 170 735 3140
Fragments arranged by size:
3140 735 170 145
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 125 633 665 1775 1949 2340 2367 3712 125
Size: 508 32 1110 174 391 27 1345 603
Fragments arranged by size:
1345 1110 603 508 391 174 32 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 705 2398 2708 2854 4000 705
Size: 1693 310 146 1146 895
Fragments arranged by size:
1693 1146 895 310 146
EcoICRI GAG'CTC
Cuts at: 1202 1202
Size: 4190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 919 1080 2015 2447 3194 3462 640
Size: 279 161 935 432 747 268 1368
Fragments arranged by size:
1368 935 747 432 279 268 161
EcoNI CCTnn'n_nnAGG
Cuts at: 793 793
Size: 4190
EcoO109I rG'GnC_Cy
Cuts at: 248 272 928 1005 1329 248
Size: 24 656 77 324 3109
Fragments arranged by size:
3109 656 324 77 24
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4190
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 150 1624 1872 2894 3873 150
Size: 1474 248 1022 979 467
Fragments arranged by size:
1474 1022 979 467 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 613 645 802 834 2032 2062 2064 2094 613
Size: 32 157 32 1198 30 2 30 2709
Fragments arranged by size:
2709 1198 157 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1229 1229
Size: 4190
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1020 1155 1210 1020
Size: 135 55 4000
Fragments arranged by size:
4000 135 55
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 617 722 2861 3045 617
Size: 105 2139 184 1762
Fragments arranged by size:
2139 1762 184 105
MscI TGG'CCA
Cuts at: 1951 1951
Size: 4190
MslI CAynn'nnrTG
Cuts at: 979 2179 2306 979
Size: 1200 127 2863
Fragments arranged by size:
2863 1200 127
MspA1I CmG'CkG
Cuts at: 177 344 540 1100 1301 1615 1975 2988 3233
Size: 167 196 560 201 314 360 1013 245
Cuts at: 3233 177
Size: 1134
Fragments arranged by size:
1134 1013 560 360 314 245 201 196
167
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NcoI C'CATG_G
Cuts at: 107 548 107
Size: 441 3749
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NspI r_CATG'y
Cuts at: 1243 1743 2650 1243
Size: 500 907 2783
Fragments arranged by size:
2783 907 500
PasI CC'CwG_GG
Cuts at: 117 795 117
Size: 678 3512
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PmlI CAC'GTG
Cuts at: 981 981
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PpuMI rG'GwC_Cy
Cuts at: 248 272 1005 248
Size: 24 733 3433
Fragments arranged by size:
3433 733 24
PspOMI G'GGCC_C
Cuts at: 120 927 120
Size: 807 3383
PstI C_TGCA'G
Cuts at: 746 1025 1139 1237 1922 746
Size: 279 114 98 685 3014
Fragments arranged by size:
3014 685 279 114 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 177 540 1100 1615 1975 177
Size: 363 560 515 360 2392
Fragments arranged by size:
2392 560 515 363 360
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 1204 1204
Size: 4190
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SanDI GG'GwC_CC
Cuts at: 248 272 248
Size: 24 4166
SapI GCTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
SbfI CC_TGCA'GG
Cuts at: 746 1025 1237 746
Size: 279 212 3699
Fragments arranged by size:
3699 279 212
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SfcI C'TryA_G
Cuts at: 33 742 1021 1135 1233 1918 2911 3102 33
Size: 709 279 114 98 685 993 191 1121
Fragments arranged by size:
1121 993 709 685 279 191 114 98
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 218 754 1345 2260 2752 3014 218
Size: 536 591 915 492 262 1394
Fragments arranged by size:
1394 915 591 536 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StuI AGG'CCT
Cuts at: 904 1123 904
Size: 219 3971
StyI C'CwwG_G
Cuts at: 107 548 1001 1324 1678 107
Size: 441 453 323 354 2619
Fragments arranged by size:
2619 453 441 354 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 662 688 1773 1906 2365 2574 662
Size: 26 1085 133 459 209 2278
Fragments arranged by size:
2278 1085 459 209 133 26
TatI w'GTAC_w
Cuts at: 446 3705 446
Size: 3259 931
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4158
Tth111I GACn'n_nGTC
Cuts at: 418 816 1987 418
Size: 398 1171 2621
Fragments arranged by size:
2621 1171 398
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 140 530 675 1685 114
Size: 26 390 145 1010 2619
Fragments arranged by size:
2619 1010 390 145 26
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
BseRI BsgI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI