Restriction map for
pF1KE2334
(Circular) (Six-base) MAPSORT of: pF1KE2334.seq Check: 6009 from: 1 to: 7778
pF1KE2334 7778 bp
With 182 enzymes: SgfI *
March 30, 2011 17:32 ..
AccI GT'mk_AC
Cuts at: 1460 4816 1460
Size: 3356 4422
Acc65I G'GTAC_C
Cuts at: 385 4794 385
Size: 4409 3369
AclI AA'CG_TT
Cuts at: 7618 7618
Size: 7778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1742 2779 2785 3236 3500 3644 3964 4393 5603
Size: 1037 6 451 264 144 320 429 1210
Cuts at: 5603 6035 6782 7050 1742
Size: 432 747 268 2470
Fragments arranged by size:
2470 1210 1037 747 451 432 429 320
268 264 144 6
AflIII A'CryG_T
Cuts at: 1869 5130 6234 7211 1869
Size: 3261 1104 977 2436
Fragments arranged by size:
3261 2436 1104 977
AgeI A'CCGG_T
Cuts at: 6166 6166
Size: 7778
AleI CACnn'nnGTG
Cuts at: 919 919
Size: 7778
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3946 3946
Size: 7778
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1742 1774 3166 3198 1742
Size: 32 1392 32 6322
Fragments arranged by size:
6322 1392 32 32
AlwNI CAG_nnn'CTG
Cuts at: 435 622 1818 2439 2644 4298 5083 6650 435
Size: 187 1196 621 205 1654 785 1567 1563
Fragments arranged by size:
1654 1567 1563 1196 785 621 205 187
ApaLI G'TGCA_C
Cuts at: 4352 6548 4352
Size: 2196 5582
ApoI r'AATT_y
Cuts at: 331 339 453 463 724 1161 3286 3324 3638
Size: 8 114 10 261 437 2125 38 314
Cuts at: 3638 3967 4782 7131 7387 331
Size: 329 815 2349 256 722
Fragments arranged by size:
2349 2125 815 722 437 329 314 261
256 114 38 10 8
AseI AT'TA_AT
Cuts at: 20 3875 20
Size: 3855 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7778
AvaI C'yCGr_G
Cuts at: 1361 4798 1361
Size: 3437 4341
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 878 911 878
Size: 33 7745
BamHI G'GATC_C
Cuts at: 3596 4803 3596
Size: 1207 6571
BanI G'GyrC_C
Cuts at: 385 1053 4494 4794 5208 5456 5491 385
Size: 668 3441 300 414 248 35 2672
Fragments arranged by size:
3441 2672 668 414 300 248 35
BanII G_rGCy'C
Cuts at: 1207 2831 4792 5822 1207
Size: 1624 1961 1030 3163
Fragments arranged by size:
3163 1961 1624 1030
BbeI G_GCGC'C
Cuts at: 5212 5460 5212
Size: 248 7530
BbsI GAAGACnn'nnnn_
Cuts at: 323 583 2699 3023 3767 3844 4732 323
Size: 260 2116 324 744 77 888 3369
Fragments arranged by size:
3369 2116 888 744 324 260 77
BciVI GTATCCnnnnn_n'
Cuts at: 5676 6437 7778 5676
Size: 761 1341 5676
Fragments arranged by size:
5676 1341 761
BclI T'GATC_A
Cuts at: 2139 2492 2786 4185 4461 5298 2139
Size: 353 294 1399 276 837 4619
Fragments arranged by size:
4619 1399 837 353 294 276
BfrBI ATG'CAT
Cuts at: 2076 2076
Size: 7778
BglI GCCn_nnn'nGGC
Cuts at: 3653 4077 3653
Size: 424 7354
BglII A'GATC_T
Cuts at: 981 1080 2534 4203 4467 5293 7330 981
Size: 99 1454 1669 264 826 2037 1429
Fragments arranged by size:
2037 1669 1454 1429 826 264 99
BlpI GC'TnA_GC
Cuts at: 3140 4890 3140
Size: 1750 6028
Bme1580I G_kGCm'C
Cuts at: 4356 5403 5496 6552 4356
Size: 1047 93 1056 5582
Fragments arranged by size:
5582 1056 1047 93
BmrI ACTGGGnnnn_n'
Cuts at: 4323 4990 5071 5406 7550 4323
Size: 667 81 335 2144 4551
Fragments arranged by size:
4551 2144 667 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 732 3134 6145 732
Size: 2402 3011 2365
Fragments arranged by size:
3011 2402 2365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 1018 1292 1519 1769 1868 1906 2006 2107
Size: 588 274 227 250 99 38 100 101
Cuts at: 2107 2523 2836 430
Size: 416 313 5372
Fragments arranged by size:
5372 588 416 313 274 250 227 101
100 99 38
Bpu10I CC'TnA_GC
Cuts at: 3269 3269
Size: 7778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 4499 4954 5833 6325 6623 619
Size: 3880 455 879 492 298 1774
Fragments arranged by size:
3880 1774 879 492 455 298
BsaI GGTCTCn'nnnn_
Cuts at: 1624 2083 7482 1624
Size: 459 5399 1920
Fragments arranged by size:
5399 1920 459
BsaAI yAC'GTr
Cuts at: 5131 5131
Size: 7778
BsaBI GATnn'nnATC
Cuts at: 6 1799 1949 4184 4466 5292 7329 6
Size: 1793 150 2235 282 826 2037 455
Fragments arranged by size:
2235 2037 1793 826 455 282 150
BsaHI Gr'CG_yC
Cuts at: 4550 5209 5457 6159 7655 4550
Size: 659 248 702 1496 4673
Fragments arranged by size:
4673 1496 702 659 248
BsaWI w'CCGG_w
Cuts at: 1466 4967 5488 6166 6440 6587 6998 1466
Size: 3501 521 678 274 147 411 2246
Fragments arranged by size:
3501 2246 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 176 206 991 1021 1742 1772 1841 1871 3648
Size: 30 785 30 721 30 69 30 1777
Cuts at: 3648 3678 3942 3972 176
Size: 30 264 30 3982
Fragments arranged by size:
3982 1777 785 721 264 69 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 210 2845 210
Size: 2635 5143
BseYI C'CCAG_C
Cuts at: 5203 5961 6538 7034 5203
Size: 758 577 496 5947
Fragments arranged by size:
5947 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1607 970
Size: 637 7141
BsiEI CG_ry'CG
Cuts at: 104 5320 5366 6574 104
Size: 5216 46 1208 1308
Fragments arranged by size:
5216 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 865 1042 1207 2831 4242 4356 4713 4792 5570
Size: 177 165 1624 1411 114 357 79 778
Cuts at: 5570 5760 6552 865
Size: 190 792 2091
Fragments arranged by size:
2091 1624 1411 792 778 357 190 177
165 114 79
BsmI GAATG_Cn'
Cuts at: 2027 2027
Size: 7778
BsmBI CGTCTCn'nnnn_
Cuts at: 116 116
Size: 7778
Bsp1286I G_dGCh'C
Cuts at: 865 1042 1207 2831 4242 4356 4713 4792 5403
Size: 177 165 1624 1411 114 357 79 611
Cuts at: 5403 5496 5570 5760 5822 6552 865
Size: 93 74 190 62 730 2091
Fragments arranged by size:
2091 1624 1411 730 611 357 190 177
165 114 93 79 74 62
BspEI T'CCGG_A
Cuts at: 1466 6998 1466
Size: 5532 2246
BspHI T'CATG_A
Cuts at: 378 1142 2162 6954 378
Size: 764 1020 4792 1202
Fragments arranged by size:
4792 1202 1020 764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4828 5042 5344 5725 4828
Size: 214 302 381 6881
Fragments arranged by size:
6881 381 302 214
BsrBI CCG'CTC
Cuts at: 50 131 2113 6070 6124 7608 7773 50
Size: 81 1982 3957 54 1484 165 55
Fragments arranged by size:
3957 1982 1484 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 768 2648 2799 3456 5690 768
Size: 1880 151 657 2234 2856
Fragments arranged by size:
2856 2234 1880 657 151
BsrFI r'CCGG_y
Cuts at: 156 5776 5957 6166 156
Size: 5620 181 209 1768
Fragments arranged by size:
5620 1768 209 181
BsrGI T'GTAC_A
Cuts at: 4715 4715
Size: 7778
BssSI C'ACGA_G
Cuts at: 6049 6407 6049
Size: 358 7420
BstAPI GCAn_nnn'nTGC
Cuts at: 1453 5155 1453
Size: 3702 4076
BstBI TT'CG_AA
Cuts at: 6139 6139
Size: 7778
BstXI CCAn_nnnn'nTGG
Cuts at: 1050 3549 3593 1050
Size: 2499 44 5235
Fragments arranged by size:
5235 2499 44
BstYI r'GATC_y
Cuts at: 1 981 1080 1149 2534 2988 3596 4124 4203
Size: 980 99 69 1385 454 608 528 79
Cuts at: 4203 4368 4467 4679 4803 5270 5293 5628 5874
Size: 165 99 212 124 467 23 335 246
Cuts at: 5874 6886 6972 6984 7330 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1385 1012 980 608 528 467 454 449
346 335 246 212 165 124 99 99
86 79 69 23 12
Bsu36I CC'TnA_GG
Cuts at: 2880 3507 2880
Size: 627 7151
BtgI C'CryG_G
Cuts at: 107 143 3487 107
Size: 36 3344 4398
Fragments arranged by size:
4398 3344 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5728 5909 6072 5728
Size: 181 163 7434
Fragments arranged by size:
7434 181 163
BtsI GCAGTG_nn'
Cuts at: 2789 5021 2789
Size: 2232 5546
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 906 941 906
Size: 35 7743
DraI TTT'AAA
Cuts at: 462 1061 2691 3206 4777 462
Size: 599 1630 515 1571 3463
Fragments arranged by size:
3463 1630 1571 599 515
DrdI GACnn_nn'nnGTC
Cuts at: 5484 6342 5484
Size: 858 6920
EaeI y'GGCC_r
Cuts at: 851 2606 3551 5363 5537 5928 5955 7300 851
Size: 1755 945 1812 174 391 27 1345 1329
Fragments arranged by size:
1812 1755 1345 1329 945 391 174 27
EagI C'GGCC_G
Cuts at: 5363 5363
Size: 7778
EarI CTCTTCn'nnn_
Cuts at: 611 1223 1291 2969 3392 4135 4269 5801 6011
Size: 612 68 1678 423 743 134 1532 210
Cuts at: 6011 611
Size: 2378
Fragments arranged by size:
2378 1678 1532 743 612 423 210 134
68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5986 6296 6442 7588 5986
Size: 310 146 1146 6176
Fragments arranged by size:
6176 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1205 2829 4790 1205
Size: 1624 1961 4193
Fragments arranged by size:
4193 1961 1624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 1018 1292 1519 1742 1769 1868 1906 2006
Size: 588 274 227 223 27 99 38 100
Cuts at: 2006 2107 2523 2779 2785 2836 3236 3500 3644
Size: 101 416 256 6 51 400 264 144
Cuts at: 3644 3964 4393 5603 6035 6782 7050 430
Size: 320 429 1210 432 747 268 1158
Fragments arranged by size:
1210 1158 747 588 432 429 416 400
320 274 268 264 256 227 223 144
101 100 99 51 38 27 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2031 2031
Size: 7778
EcoO109I rG'GnC_Cy
Cuts at: 2628 4437 4750 4917 2628
Size: 1809 313 167 5489
Fragments arranged by size:
5489 1809 313 167
EcoRI G'AATT_C
Cuts at: 4782 4782
Size: 7778
EcoRV GAT'ATC
Cuts at: 664 664
Size: 7778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1564 4005 4146 4540 5797 1564
Size: 2441 141 394 1257 3545
Fragments arranged by size:
3545 2441 1257 394 141
FspI TGC'GCA
Cuts at: 5559 5559
Size: 7778
HaeII r_GCGC'y
Cuts at: 5212 5460 6482 7461 5212
Size: 248 1022 979 5529
Fragments arranged by size:
5529 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1086 1118 2264 2296 2484 2516 3581 3613 3778
Size: 32 1146 32 188 32 1065 32 165
Cuts at: 3778 3810 4537 4569 5620 5650 5652 5682 1086
Size: 32 727 32 1051 30 2 30 3182
Fragments arranged by size:
3182 1146 1065 1051 727 188 165 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1491 3087 4161 4817 1491
Size: 1596 1074 656 4452
Fragments arranged by size:
4452 1596 1074 656
HindIII A'AGCT_T
Cuts at: 3210 3210
Size: 7778
KasI G'GCGC_C
Cuts at: 5208 5456 5208
Size: 248 7530
KpnI G_GTAC'C
Cuts at: 389 4798 389
Size: 4409 3369
MluI A'CGCG_T
Cuts at: 7211 7211
Size: 7778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1971 2600 6449 6633 1971
Size: 629 3849 184 3116
Fragments arranged by size:
3849 3116 629 184
MscI TGG'CCA
Cuts at: 853 2608 3553 5539 853
Size: 1755 945 1986 3092
Fragments arranged by size:
3092 1986 1755 945
MslI CAynn'nnrTG
Cuts at: 919 2071 2602 3547 3928 4349 5767 5894 919
Size: 1152 531 945 381 421 1418 127 2803
Fragments arranged by size:
2803 1418 1152 945 531 421 381 127
MspA1I CmG'CkG
Cuts at: 145 523 1198 1657 2212 3144 3573 4624 4889
Size: 378 675 459 555 932 429 1051 265
Cuts at: 4889 5203 5563 6576 6821 145
Size: 314 360 1013 245 1102
Fragments arranged by size:
1102 1051 1013 932 675 555 459 429
378 360 314 265 245
NaeI GCC'GGC
Cuts at: 5959 5959
Size: 7778
NarI GG'CG_CC
Cuts at: 5209 5457 5209
Size: 248 7530
NcoI C'CATG_G
Cuts at: 107 107
Size: 7778
NdeI CA'TA_TG
Cuts at: 3372 3372
Size: 7778
NgoMIV G'CCGG_C
Cuts at: 5957 5957
Size: 7778
NsiI A_TGCA'T
Cuts at: 2078 2078
Size: 7778
NspI r_CATG'y
Cuts at: 1873 3201 4831 5331 6238 1873
Size: 1328 1630 500 907 3413
Fragments arranged by size:
3413 1630 1328 907 500
PciI A'CATG_T
Cuts at: 1869 6234 1869
Size: 4365 3413
PflMI CCAn_nnn'nTGG
Cuts at: 1791 1791
Size: 7778
PfoI T'CCnGG_A
Cuts at: 1883 2084 4133 4637 1883
Size: 201 2049 504 5024
Fragments arranged by size:
5024 2049 504 201
PmeI GTTT'AAAC
Cuts at: 4777 4777
Size: 7778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1742 1774 6917 6949 1742
Size: 32 5143 32 2571
Fragments arranged by size:
5143 2571 32 32
PpuMI rG'GwC_Cy
Cuts at: 4437 4750 4437
Size: 313 7465
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 188 220 188
Size: 32 7746
PstI C_TGCA'G
Cuts at: 2625 2640 2749 4825 5510 2625
Size: 15 109 2076 685 4893
Fragments arranged by size:
4893 2076 685 109 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7778
PvuII CAG'CTG
Cuts at: 523 1198 1657 2212 3144 3573 4624 5203 5563
Size: 675 459 555 932 429 1051 579 360
Cuts at: 5563 523
Size: 2738
Fragments arranged by size:
2738 1051 932 675 579 555 459 429
360
RsrII CG'GwC_CG
Cuts at: 5973 5973
Size: 7778
SacI G_AGCT'C
Cuts at: 1207 2831 4792 1207
Size: 1624 1961 4193
Fragments arranged by size:
4193 1961 1624
SacII CC_GC'GG
Cuts at: 146 146
Size: 7778
SalI G'TCGA_C
Cuts at: 4815 4815
Size: 7778
SapI GCTCTTCn'nnn_
Cuts at: 611 2969 5801 6011 611
Size: 2358 2832 210 2378
Fragments arranged by size:
2832 2378 2358 210
SbfI CC_TGCA'GG
Cuts at: 4825 4825
Size: 7778
ScaI AGT'ACT
Cuts at: 7295 7295
Size: 7778
SfcI C'TryA_G
Cuts at: 33 2309 2621 2636 2745 3496 4290 4675 4821
Size: 2276 312 15 109 751 794 385 146
Cuts at: 4821 5506 6499 6690 33
Size: 685 993 191 1121
Fragments arranged by size:
2276 1121 993 794 751 685 385 312
191 146 109 15
SfoI GGC'GCC
Cuts at: 5210 5458 5210
Size: 248 7530
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7778
SmaI CCC'GGG
Cuts at: 4800 4800
Size: 7778
SmlI C'TyrA_G
Cuts at: 598 4514 4933 5848 6340 6602 598
Size: 3916 419 915 492 262 1774
Fragments arranged by size:
3916 1774 915 492 419 262
SpeI A'CTAG_T
Cuts at: 67 4152 67
Size: 4085 3693
SphI G_CATG'C
Cuts at: 4831 5331 4831
Size: 500 7278
SspI AAT'ATT
Cuts at: 363 413 3387 3879 363
Size: 50 2974 492 4262
Fragments arranged by size:
4262 2974 492 50
StuI AGG'CCT
Cuts at: 1400 1400
Size: 7778
StyI C'CwwG_G
Cuts at: 107 878 2292 2354 2609 4422 4912 5266 107
Size: 771 1414 62 255 1813 490 354 2619
Fragments arranged by size:
2619 1813 1414 771 490 354 255 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2113 5361 5494 5953 6162 2113
Size: 3248 133 459 209 3729
Fragments arranged by size:
3729 3248 459 209 133
TatI w'GTAC_w
Cuts at: 195 869 2117 2741 4061 4140 4715 7293 195
Size: 674 1248 624 1320 79 575 2578 680
Fragments arranged by size:
2578 1320 1248 680 674 624 575 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1841 1873 3648 3680 7295 7327 1841
Size: 32 1775 32 3615 32 2292
Fragments arranged by size:
3615 2292 1775 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3091 5575 3091
Size: 2484 5294
XbaI T'CTAG_A
Cuts at: 4809 4809
Size: 7778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2393 5273 2393
Size: 2880 4898
XmaI C'CCGG_G
Cuts at: 4798 4798
Size: 7778
XmnI GAAnn'nnTTC
Cuts at: 1243 2193 3914 1243
Size: 950 1721 5107
Fragments arranged by size:
5107 1721 950
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BbvCI BcgI BmgBI BmtI BsiWI BssHII BstEII BstZ17I
ClaI DraIII FseI FspAI HpaI MfeI NheI NotI
NruI PacI PasI PmlI PshAI PsiI PspOMI PspXI
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI