Restriction map for
pF1KE2329
(Circular) (Six-base) MAPSORT of: pF1KE2329.seq Check: 9437 from: 1 to: 7052
pF1KE2329 7052 bp
With 182 enzymes: SgfI *
February 23, 2011 17:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1225 3735 1225
Size: 2510 4542
AccI GT'mk_AC
Cuts at: 871 1345 1372 3817 4090 871
Size: 474 27 2445 273 3833
Fragments arranged by size:
3833 2445 474 273 27
Acc65I G'GTAC_C
Cuts at: 4068 4068
Size: 7052
AclI AA'CG_TT
Cuts at: 6892 6892
Size: 7052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1688 1940 2279 3362 3539 4001 4877 5309
Size: 1072 252 339 1083 177 462 876 432
Cuts at: 5309 6056 6324 616
Size: 747 268 1344
Fragments arranged by size:
1344 1083 1072 876 747 462 432 339
268 252 177
AflIII A'CryG_T
Cuts at: 4404 5508 6485 4404
Size: 1104 977 4971
Fragments arranged by size:
4971 1104 977
AgeI A'CCGG_T
Cuts at: 5440 5440
Size: 7052
AhdI GACnn_n'nnGTC
Cuts at: 2743 2743
Size: 7052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3239 3428 3239
Size: 189 6863
AlwNI CAG_nnn'CTG
Cuts at: 1927 2660 4357 5924 1927
Size: 733 1697 1567 3055
Fragments arranged by size:
3055 1697 1567 733
ApaI G_GGCC'C
Cuts at: 581 3181 581
Size: 2600 4452
ApaLI G'TGCA_C
Cuts at: 3959 5822 3959
Size: 1863 5189
ApoI r'AATT_y
Cuts at: 1405 1536 1641 1719 2050 2503 4056 6405 6661
Size: 131 105 78 331 453 1553 2349 256
Cuts at: 6661 1405
Size: 1796
Fragments arranged by size:
2349 1796 1553 453 331 256 131 105
78
AscI GG'CGCG_CC
Cuts at: 529 529
Size: 7052
AseI AT'TA_AT
Cuts at: 20 20
Size: 7052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7052
AvaI C'yCGr_G
Cuts at: 184 246 447 1475 1835 2112 4072 184
Size: 62 201 1028 360 277 1960 3164
Fragments arranged by size:
3164 1960 1028 360 277 201 62
BamHI G'GATC_C
Cuts at: 1296 4077 1296
Size: 2781 4271
BanI G'GyrC_C
Cuts at: 451 490 3339 4068 4482 4730 4765 451
Size: 39 2849 729 414 248 35 2738
Fragments arranged by size:
2849 2738 729 414 248 39 35
BanII G_rGCy'C
Cuts at: 313 581 1226 1675 3034 3181 3399 3490 4066
Size: 268 645 449 1359 147 218 91 576
Cuts at: 4066 5096 313
Size: 1030 2269
Fragments arranged by size:
2269 1359 1030 645 576 449 268 218
147 91
BbeI G_GCGC'C
Cuts at: 455 494 4486 4734 455
Size: 39 3992 248 2773
Fragments arranged by size:
3992 2773 248 39
BbsI GAAGACnn'nnnn_
Cuts at: 1946 1946
Size: 7052
BbvCI CC'TCA_GC
Cuts at: 3236 3548 3236
Size: 312 6740
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 7018
BciVI GTATCCnnnnn_n'
Cuts at: 266 2067 3026 4950 5711 7052 266
Size: 1801 959 1924 761 1341 266
Fragments arranged by size:
1924 1801 1341 959 761 266
BclI T'GATC_A
Cuts at: 2600 4572 2600
Size: 1972 5080
BfrBI ATG'CAT
Cuts at: 3424 3424
Size: 7052
BglI GCCn_nnn'nGGC
Cuts at: 189 213 460 1866 3646 189
Size: 24 247 1406 1780 3595
Fragments arranged by size:
3595 1780 1406 247 24
BglII A'GATC_T
Cuts at: 4567 6604 4567
Size: 2037 5015
BlpI GC'TnA_GC
Cuts at: 114 2825 3497 4164 114
Size: 2711 672 667 3002
Fragments arranged by size:
3002 2711 672 667
Bme1580I G_kGCm'C
Cuts at: 581 1382 1464 1559 3181 3963 4677 4770 5826
Size: 801 82 95 1622 782 714 93 1056
Cuts at: 5826 581
Size: 1807
Fragments arranged by size:
1807 1622 1056 801 782 714 95 93
82
BmrI ACTGGGnnnn_n'
Cuts at: 1458 2066 2207 3019 3605 4264 4345 4680 6824
Size: 608 141 812 586 659 81 335 2144
Cuts at: 6824 1458
Size: 1686
Fragments arranged by size:
2144 1686 812 659 608 586 335 141
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 332 5419 332
Size: 5087 1965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1125 1240 1848 1904 2075 2957 184
Size: 941 115 608 56 171 882 4279
Fragments arranged by size:
4279 941 882 608 171 115 56
Bpu10I CC'TnA_GC
Cuts at: 2627 3236 3548 2627
Size: 609 312 6131
Fragments arranged by size:
6131 609 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 2912 4228 5107 5599 5897 1282
Size: 1630 1316 879 492 298 2437
Fragments arranged by size:
2437 1630 1316 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 400 768 2186 6756 400
Size: 368 1418 4570 696
Fragments arranged by size:
4570 1418 696 368
BsaAI yAC'GTr
Cuts at: 1349 3843 4405 1349
Size: 2494 562 3996
Fragments arranged by size:
3996 2494 562
BsaBI GATnn'nnATC
Cuts at: 6 4566 6603 6
Size: 4560 2037 455
Fragments arranged by size:
4560 2037 455
BsaHI Gr'CG_yC
Cuts at: 452 491 518 3067 4483 4731 5433 6929 452
Size: 39 27 2549 1416 248 702 1496 575
Fragments arranged by size:
2549 1496 1416 702 575 248 39 27
BsaWI w'CCGG_w
Cuts at: 4241 4762 5440 5714 5861 6272 4241
Size: 521 678 274 147 411 5021
Fragments arranged by size:
5021 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 119 756 780 2078 119
Size: 637 24 1298 5093
Fragments arranged by size:
5093 1298 637 24
BseYI C'CCAG_C
Cuts at: 602 1988 2020 2692 2775 3162 3356 3762 4477
Size: 1386 32 672 83 387 194 406 715
Cuts at: 4477 5235 5812 6308 602
Size: 758 577 496 1346
Fragments arranged by size:
1386 1346 758 715 672 577 496 406
387 194 83 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3511 3590 3511
Size: 79 6973
BsiEI CG_ry'CG
Cuts at: 104 122 208 464 1858 4594 4640 5848 104
Size: 18 86 256 1394 2736 46 1208 1308
Fragments arranged by size:
2736 1394 1308 1208 256 86 46 18
BsiHKAI G_wGCw'C
Cuts at: 313 2729 3963 4066 4844 5034 5826 313
Size: 2416 1234 103 778 190 792 1539
Fragments arranged by size:
2416 1539 1234 792 778 190 103
BsmBI CGTCTCn'nnnn_
Cuts at: 525 1901 525
Size: 1376 5676
Bsp1286I G_dGCh'C
Cuts at: 313 581 1226 1382 1464 1559 1675 2729 3034
Size: 268 645 156 82 95 116 1054 305
Cuts at: 3034 3181 3399 3490 3963 4066 4677 4770 4844
Size: 147 218 91 473 103 611 93 74
Cuts at: 4844 5034 5096 5826 313
Size: 190 62 730 1539
Fragments arranged by size:
1539 1054 730 645 611 473 305 268
218 190 156 147 116 103 95 93
91 82 74 62
BspEI T'CCGG_A
Cuts at: 6272 6272
Size: 7052
BspHI T'CATG_A
Cuts at: 3399 6228 3399
Size: 2829 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1225 3735 4102 4316 4618 4999 1225
Size: 2510 367 214 302 381 3278
Fragments arranged by size:
3278 2510 381 367 302 214
BsrBI CCG'CTC
Cuts at: 50 221 486 5344 5398 6882 7047 50
Size: 171 265 4858 54 1484 165 55
Fragments arranged by size:
4858 1484 265 171 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4964 4964
Size: 7052
BsrFI r'CCGG_y
Cuts at: 207 567 3810 5050 5231 5440 207
Size: 360 3243 1240 181 209 1819
Fragments arranged by size:
3243 1819 1240 360 209 181
BsrGI T'GTAC_A
Cuts at: 3858 3858
Size: 7052
BssHII G'CGCG_C
Cuts at: 529 529
Size: 7052
BssSI C'ACGA_G
Cuts at: 5323 5681 5323
Size: 358 6694
BstAPI GCAn_nnn'nTGC
Cuts at: 4429 4429
Size: 7052
BstBI TT'CG_AA
Cuts at: 5413 5413
Size: 7052
BstXI CCAn_nnnn'nTGG
Cuts at: 3592 3592
Size: 7052
BstYI r'GATC_y
Cuts at: 1 1296 1687 4077 4544 4567 4902 5148 6160
Size: 1295 391 2390 467 23 335 246 1012
Cuts at: 6160 6246 6258 6604 1
Size: 86 12 346 449
Fragments arranged by size:
2390 1295 1012 467 449 391 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1373 3818 1373
Size: 2445 4607
Bsu36I CC'TnA_GG
Cuts at: 2741 3848 2741
Size: 1107 5945
BtgI C'CryG_G
Cuts at: 140 680 779 3482 140
Size: 540 99 2703 3710
Fragments arranged by size:
3710 2703 540 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1751 3430 5002 5183 5346 1751
Size: 1679 1572 181 163 3457
Fragments arranged by size:
3457 1679 1572 181 163
BtsI GCAGTG_nn'
Cuts at: 4295 4295
Size: 7052
DraI TTT'AAA
Cuts at: 990 1128 1412 2498 4051 990
Size: 138 284 1086 1553 3991
Fragments arranged by size:
3991 1553 1086 284 138
DraIII CAC_nnn'GTG
Cuts at: 2074 2074
Size: 7052
DrdI GACnn_nn'nnGTC
Cuts at: 4758 5616 4758
Size: 858 6194
EaeI y'GGCC_r
Cuts at: 119 146 205 461 776 1762 1855 1879 3659
Size: 27 59 256 315 986 93 24 1780
Cuts at: 3659 4637 4811 5202 5229 6574 119
Size: 978 174 391 27 1345 597
Fragments arranged by size:
1780 1345 986 978 597 391 315 256
174 93 59 27 27 24
EagI C'GGCC_G
Cuts at: 119 205 461 1855 4637 119
Size: 86 256 1394 2782 2534
Fragments arranged by size:
2782 2534 1394 256 86
EarI CTCTTCn'nnn_
Cuts at: 1832 2801 5075 5285 1832
Size: 969 2274 210 3599
Fragments arranged by size:
3599 2274 969 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 231 252 312 5260 5570 5716 6862 231
Size: 21 60 4948 310 146 1146 421
Fragments arranged by size:
4948 1146 421 310 146 60 21
EcoICRI GAG'CTC
Cuts at: 311 4064 311
Size: 3753 3299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 616 1125 1240 1688 1848 1904 1940 2075
Size: 432 509 115 448 160 56 36 135
Cuts at: 2075 2279 2957 3362 3539 4001 4877 5309 6056
Size: 204 678 405 177 462 876 432 747
Cuts at: 6056 6324 184
Size: 268 912
Fragments arranged by size:
912 876 747 678 509 462 448 432
432 405 268 204 177 160 135 115
56 36
EcoO109I rG'GnC_Cy
Cuts at: 578 1215 1816 1868 1963 2737 4191 578
Size: 637 601 52 95 774 1454 3439
Fragments arranged by size:
3439 1454 774 637 601 95 52
EcoRI G'AATT_C
Cuts at: 1641 1719 4056 1641
Size: 78 2337 4637
Fragments arranged by size:
4637 2337 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1178 1709 2555 3479 5071 1178
Size: 531 846 924 1592 3159
Fragments arranged by size:
3159 1592 924 846 531
FspI TGC'GCA
Cuts at: 3509 4833 3509
Size: 1324 5728
FspAI rTGC'GCAy
Cuts at: 3509 3509
Size: 7052
HaeII r_GCGC'y
Cuts at: 195 455 494 4486 4734 5756 6735 195
Size: 260 39 3992 248 1022 979 512
Fragments arranged by size:
3992 1022 979 512 260 248 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1123 1155 2585 2617 2729 2761 4894 4924 4926
Size: 32 1430 32 112 32 2133 30 2
Cuts at: 4926 4956 1123
Size: 30 3219
Fragments arranged by size:
3219 2133 1430 112 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 4091 4091
Size: 7052
KasI G'GCGC_C
Cuts at: 451 490 4482 4730 451
Size: 39 3992 248 2773
Fragments arranged by size:
3992 2773 248 39
KpnI G_GTAC'C
Cuts at: 4072 4072
Size: 7052
MluI A'CGCG_T
Cuts at: 6485 6485
Size: 7052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2278 3758 3910 5723 5907 2278
Size: 1480 152 1813 184 3423
Fragments arranged by size:
3423 1813 1480 184 152
MscI TGG'CCA
Cuts at: 148 1764 3661 4813 148
Size: 1616 1897 1152 2387
Fragments arranged by size:
2387 1897 1616 1152
MslI CAynn'nnrTG
Cuts at: 3076 3146 3296 5041 5168 3076
Size: 70 150 1745 127 4960
Fragments arranged by size:
4960 1745 150 127 70
MspA1I CmG'CkG
Cuts at: 118 142 204 682 713 2284 2824 3126 3175
Size: 24 62 478 31 1571 540 302 49
Cuts at: 3175 4163 4477 4837 5850 6095 118
Size: 988 314 360 1013 245 1075
Fragments arranged by size:
1571 1075 1013 988 540 478 360 314
302 245 62 49 31 24
NaeI GCC'GGC
Cuts at: 209 3812 5233 209
Size: 3603 1421 2028
Fragments arranged by size:
3603 2028 1421
NarI GG'CG_CC
Cuts at: 452 491 4483 4731 452
Size: 39 3992 248 2773
Fragments arranged by size:
3992 2773 248 39
NgoMIV G'CCGG_C
Cuts at: 207 3810 5231 207
Size: 3603 1421 2028
Fragments arranged by size:
3603 2028 1421
NotI GC'GGCC_GC
Cuts at: 119 119
Size: 7052
NsiI A_TGCA'T
Cuts at: 3426 3426
Size: 7052
NspI r_CATG'y
Cuts at: 1953 2783 3271 3415 3428 4105 4605 5512 1953
Size: 830 488 144 13 677 500 907 3493
Fragments arranged by size:
3493 907 830 677 500 488 144 13
PasI CC'CwG_GG
Cuts at: 1226 1226
Size: 7052
PciI A'CATG_T
Cuts at: 5508 5508
Size: 7052
PfoI T'CCnGG_A
Cuts at: 1593 1593
Size: 7052
PmeI GTTT'AAAC
Cuts at: 4051 4051
Size: 7052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6191 6223 6191
Size: 32 7020
PpuMI rG'GwC_Cy
Cuts at: 1215 1816 2737 1215
Size: 601 921 5530
Fragments arranged by size:
5530 921 601
PshAI GACnn'nnGTC
Cuts at: 1893 2178 1893
Size: 285 6767
PspOMI G'GGCC_C
Cuts at: 577 3177 577
Size: 2600 4452
PspXI vC'TCGA_Gb
Cuts at: 1475 1475
Size: 7052
PstI C_TGCA'G
Cuts at: 2388 4099 4784 2388
Size: 1711 685 4656
Fragments arranged by size:
4656 1711 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7052
PvuII CAG'CTG
Cuts at: 2284 2824 4477 4837 2284
Size: 540 1653 360 4499
Fragments arranged by size:
4499 1653 540 360
RsrII CG'GwC_CG
Cuts at: 5247 5247
Size: 7052
SacI G_AGCT'C
Cuts at: 313 4066 313
Size: 3753 3299
SacII CC_GC'GG
Cuts at: 143 683 143
Size: 540 6512
SalI G'TCGA_C
Cuts at: 4089 4089
Size: 7052
SapI GCTCTTCn'nnn_
Cuts at: 5075 5285 5075
Size: 210 6842
SbfI CC_TGCA'GG
Cuts at: 2388 4099 2388
Size: 1711 5341
ScaI AGT'ACT
Cuts at: 6569 6569
Size: 7052
SfcI C'TryA_G
Cuts at: 33 2384 3228 3303 3964 4095 4780 5773 5964
Size: 2351 844 75 661 131 685 993 191
Cuts at: 5964 33
Size: 1121
Fragments arranged by size:
2351 1121 993 844 685 661 191 131
75
SfoI GGC'GCC
Cuts at: 453 492 4484 4732 453
Size: 39 3992 248 2773
Fragments arranged by size:
3992 2773 248 39
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7052
SmaI CCC'GGG
Cuts at: 186 248 449 4074 186
Size: 62 201 3625 3164
Fragments arranged by size:
3625 3164 201 62
SmlI C'TyrA_G
Cuts at: 1261 1475 2927 4207 5122 5614 5876 1261
Size: 214 1452 1280 915 492 262 2437
Fragments arranged by size:
2437 1452 1280 915 492 262 214
SnaBI TAC'GTA
Cuts at: 1349 1349
Size: 7052
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7052
SphI G_CATG'C
Cuts at: 3415 4105 4605 3415
Size: 690 500 5862
Fragments arranged by size:
5862 690 500
SrfI GCCC'GGGC
Cuts at: 449 449
Size: 7052
SspI AAT'ATT
Cuts at: 2403 2403
Size: 7052
StyI C'CwwG_G
Cuts at: 390 524 1969 2754 4186 4540 390
Size: 134 1445 785 1432 354 2902
Fragments arranged by size:
2902 1445 1432 785 354 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 750 2085 3176 3545 3624 4635 4768 5227 5436
Size: 1335 1091 369 79 1011 133 459 209
Cuts at: 5436 750
Size: 2366
Fragments arranged by size:
2366 1335 1091 1011 459 369 209 133
79
TatI w'GTAC_w
Cuts at: 951 1042 1466 3858 4020 6567 951
Size: 91 424 2392 162 2547 1436
Fragments arranged by size:
2547 2392 1436 424 162 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6569 6601 6569
Size: 32 7020
Tth111I GACn'n_nGTC
Cuts at: 4849 4849
Size: 7052
XbaI T'CTAG_A
Cuts at: 4083 4083
Size: 7052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4547 4547
Size: 7052
XhoI C'TCGA_G
Cuts at: 1475 1475
Size: 7052
XmaI C'CCGG_G
Cuts at: 184 246 447 4072 184
Size: 62 201 3625 3164
Fragments arranged by size:
3625 3164 201 62
XmnI GAAnn'nnTTC
Cuts at: 3757 3757
Size: 7052
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NsiI NspI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AvrII BaeI BmgBI
BmtI BsaXI BsiWI BsmI BstEII ClaI CspCI EcoNI
EcoRV FseI HindIII HpaI MfeI NcoI NdeI NheI
NruI PacI PflMI PmlI PsiI PsrI SanDI SexAI
SfiI SgrAI StuI SwaI ZraI