Restriction map for
pF1KE2328
(Circular) (Six-base) MAPSORT of: pF1KE2328.seq Check: 6273 from: 1 to: 4178
pF1KE2328 4178 bp
With 182 enzymes: SgfI *
February 11, 2011 17:15 ..
AccI GT'mk_AC
Cuts at: 242 1216 242
Size: 974 3204
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 2003 2435 3182 3450 1033
Size: 970 432 747 268 1761
Fragments arranged by size:
1761 970 747 432 268
AfeI AGC'GCT
Cuts at: 178 178
Size: 4178
AflIII A'CryG_T
Cuts at: 689 1530 2634 3611 689
Size: 841 1104 977 1256
Fragments arranged by size:
1256 1104 977 841
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AhdI GACnn_n'nnGTC
Cuts at: 132 132
Size: 4178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 685 685
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 227 735 825 1483 3050 227
Size: 508 90 658 1567 1355
Fragments arranged by size:
1567 1355 658 508 90
ApaLI G'TGCA_C
Cuts at: 1101 2948 1101
Size: 1847 2331
ApoI r'AATT_y
Cuts at: 1182 3531 3787 1182
Size: 2349 256 1573
Fragments arranged by size:
2349 1573 256
AseI AT'TA_AT
Cuts at: 20 955 20
Size: 935 3243
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 118 139 1198 118
Size: 21 1059 3098
Fragments arranged by size:
3098 1059 21
BamHI G'GATC_C
Cuts at: 1203 1203
Size: 4178
BanI G'GyrC_C
Cuts at: 1194 1608 1856 1891 1194
Size: 414 248 35 3481
Fragments arranged by size:
3481 414 248 35
BanII G_rGCy'C
Cuts at: 1192 2222 1192
Size: 1030 3148
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 163 587 896 163
Size: 424 309 3445
Fragments arranged by size:
3445 424 309
BbvCI CC'TCA_GC
Cuts at: 217 217
Size: 4178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4144
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2837 4178 2076
Size: 761 1341 2076
Fragments arranged by size:
2076 1341 761
BclI T'GATC_A
Cuts at: 1698 1698
Size: 4178
BglII A'GATC_T
Cuts at: 1693 3730 1693
Size: 2037 2141
BlpI GC'TnA_GC
Cuts at: 826 883 1290 826
Size: 57 407 3714
Fragments arranged by size:
3714 407 57
Bme1580I G_kGCm'C
Cuts at: 1090 1105 1803 1896 2952 1090
Size: 15 698 93 1056 2316
Fragments arranged by size:
2316 1056 698 93 15
BmrI ACTGGGnnnn_n'
Cuts at: 159 1390 1471 1806 3950 159
Size: 1231 81 335 2144 387
Fragments arranged by size:
2144 1231 387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1143 2545 1143
Size: 1402 2776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 601
Size: 4178
Bpu10I CC'TnA_GC
Cuts at: 217 217
Size: 4178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1354 2233 2725 3023 1354
Size: 879 492 298 2509
Fragments arranged by size:
2509 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3882 3882
Size: 4178
BsaAI yAC'GTr
Cuts at: 471 879 1531 471
Size: 408 652 3118
Fragments arranged by size:
3118 652 408
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1367 1888 2566 2840 2987 3398 1367
Size: 521 678 274 147 411 2147
Fragments arranged by size:
2147 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 913 943 913
Size: 30 4148
BseYI C'CCAG_C
Cuts at: 1603 2361 2938 3434 1603
Size: 758 577 496 2347
Fragments arranged by size:
2347 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 859
Size: 4178
BsiEI CG_ry'CG
Cuts at: 104 164 1720 1766 2974 104
Size: 60 1556 46 1208 1308
Fragments arranged by size:
1556 1308 1208 60 46
BsiHKAI G_wGCw'C
Cuts at: 884 1105 1192 1970 2160 2952 884
Size: 221 87 778 190 792 2110
Fragments arranged by size:
2110 792 778 221 190 87
BsmBI CGTCTCn'nnnn_
Cuts at: 703 703
Size: 4178
Bsp1286I G_dGCh'C
Cuts at: 884 1090 1105 1192 1803 1896 1970 2160 2222
Size: 206 15 87 611 93 74 190 62
Cuts at: 2222 2952 884
Size: 730 2110
Fragments arranged by size:
2110 730 611 206 190 93 87 74
62 15
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 542 3354 542
Size: 2812 1366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 797 881 1228 1442 1744 2125 797
Size: 84 347 214 302 381 2850
Fragments arranged by size:
2850 381 347 302 214 84
BsrBI CCG'CTC
Cuts at: 50 197 936 2470 2524 4008 4173 50
Size: 147 739 1534 54 1484 165 55
Fragments arranged by size:
1534 1484 739 165 147 55 54
BsrDI GCAATG_nn'
Cuts at: 2090 2090
Size: 4178
BsrFI r'CCGG_y
Cuts at: 893 2176 2357 2566 893
Size: 1283 181 209 2505
Fragments arranged by size:
2505 1283 209 181
BsrGI T'GTAC_A
Cuts at: 640 640
Size: 4178
BssSI C'ACGA_G
Cuts at: 2449 2807 2449
Size: 358 3820
BstAPI GCAn_nnn'nTGC
Cuts at: 227 1555 227
Size: 1328 2850
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstYI r'GATC_y
Cuts at: 1 356 941 1203 1670 1693 2028 2274 3286
Size: 355 585 262 467 23 335 246 1012
Cuts at: 3286 3372 3384 3730 1
Size: 86 12 346 449
Fragments arranged by size:
1012 585 467 449 355 346 335 262
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 243 243
Size: 4178
BtgI C'CryG_G
Cuts at: 390 390
Size: 4178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 510 2128 2309 2472 510
Size: 1618 181 163 2216
Fragments arranged by size:
2216 1618 181 163
BtsI GCAGTG_nn'
Cuts at: 1103 1421 1103
Size: 318 3860
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 161 1763 1937 2328 2355 3700 161
Size: 1602 174 391 27 1345 639
Fragments arranged by size:
1602 1345 639 391 174 27
EagI C'GGCC_G
Cuts at: 161 1763 161
Size: 1602 2576
EarI CTCTTCn'nnn_
Cuts at: 187 289 2201 2411 187
Size: 102 1912 210 1954
Fragments arranged by size:
1954 1912 210 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1190 1190
Size: 4178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1033 2003 2435 3182 3450 601
Size: 432 970 432 747 268 1329
Fragments arranged by size:
1329 970 747 432 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 121 856 121
Size: 735 3443
EcoO109I rG'GnC_Cy
Cuts at: 840 1317 840
Size: 477 3701
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 4178
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 180 390 1612 1860 2882 3861 180
Size: 210 1222 248 1022 979 497
Fragments arranged by size:
1222 1022 979 497 248 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 2020 2050 2052 2082 118
Size: 32 1870 30 2 30 2214
Fragments arranged by size:
2214 1870 32 30 30 2
HincII GTy'rAC
Cuts at: 1217 1217
Size: 4178
HindIII A'AGCT_T
Cuts at: 413 413
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 464 2849 3033 464
Size: 2385 184 1609
Fragments arranged by size:
2385 1609 184
MscI TGG'CCA
Cuts at: 1939 1939
Size: 4178
MslI CAynn'nnrTG
Cuts at: 636 688 694 2167 2294 636
Size: 52 6 1473 127 2520
Fragments arranged by size:
2520 1473 127 52 6
MspA1I CmG'CkG
Cuts at: 735 978 1289 1603 1963 2976 3221 735
Size: 243 311 314 360 1013 245 1692
Fragments arranged by size:
1692 1013 360 314 311 245 243
NaeI GCC'GGC
Cuts at: 2359 2359
Size: 4178
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 390 390
Size: 4178
NgoMIV G'CCGG_C
Cuts at: 2357 2357
Size: 4178
NspI r_CATG'y
Cuts at: 312 693 1231 1731 2638 312
Size: 381 538 500 907 1852
Fragments arranged by size:
1852 907 538 500 381
PasI CC'CwG_GG
Cuts at: 405 405
Size: 4178
PciI A'CATG_T
Cuts at: 689 2634 689
Size: 1945 2233
PfoI T'CCnGG_A
Cuts at: 182 1078 182
Size: 896 3282
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PmlI CAC'GTG
Cuts at: 879 879
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PpuMI rG'GwC_Cy
Cuts at: 840 840
Size: 4178
PstI C_TGCA'G
Cuts at: 1225 1910 1225
Size: 685 3493
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 735 978 1603 1963 735
Size: 243 625 360 2950
Fragments arranged by size:
2950 625 360 243
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 1192 1192
Size: 4178
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 187 2201 2411 187
Size: 2014 210 1954
Fragments arranged by size:
2014 1954 210
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 378 3695 378
Size: 3317 861
SexAI A'CCwGG_T
Cuts at: 842 842
Size: 4178
SfcI C'TryA_G
Cuts at: 33 1221 1906 2899 3090 33
Size: 1188 685 993 191 1121
Fragments arranged by size:
1188 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 141 1200 141
Size: 1059 3119
SmlI C'TyrA_G
Cuts at: 1333 2248 2740 3002 1333
Size: 915 492 262 2509
Fragments arranged by size:
2509 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
SspI AAT'ATT
Cuts at: 549 549
Size: 4178
StuI AGG'CCT
Cuts at: 759 773 759
Size: 14 4164
StyI C'CwwG_G
Cuts at: 390 1021 1312 1666 390
Size: 631 291 354 2902
Fragments arranged by size:
2902 631 354 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 141 684 1761 1894 2353 2562 141
Size: 543 1077 133 459 209 1757
Fragments arranged by size:
1757 1077 543 459 209 133
TatI w'GTAC_w
Cuts at: 376 640 3693 376
Size: 264 3053 861
Fragments arranged by size:
3053 861 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1060 1080 1092 1112 3695 3727 1060
Size: 20 12 20 2583 32 1511
Fragments arranged by size:
2583 1511 32 20 20 12
Tth111I GACn'n_nGTC
Cuts at: 844 1975 844
Size: 1131 3047
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1673 1673
Size: 4178
XmaI C'CCGG_G
Cuts at: 139 1198 139
Size: 1059 3119
XmnI GAAnn'nnTTC
Cuts at: 178 259 178
Size: 81 4097
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI ApaI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BseRI BsiWI BsmI
BssHII BstEII BstXI Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI