Restriction map for
pF1KE2326
(Circular) (Six-base) MAPSORT of: pF1KE2326.seq Check: 3898 from: 1 to: 4691
pF1KE2326 4691 bp
With 182 enzymes: SgfI *
February 11, 2011 17:15 ..
AatII G_ACGT'C
Cuts at: 1427 1427
Size: 4691
AccI GT'mk_AC
Cuts at: 1729 1729
Size: 4691
Acc65I G'GTAC_C
Cuts at: 1707 1707
Size: 4691
AclI AA'CG_TT
Cuts at: 4531 4531
Size: 4691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 738 1406 1520 2516 2948 3695 3963 738
Size: 668 114 996 432 747 268 1466
Fragments arranged by size:
1466 996 747 668 432 268 114
AflIII A'CryG_T
Cuts at: 2043 3147 4124 2043
Size: 1104 977 2610
Fragments arranged by size:
2610 1104 977
AgeI A'CCGG_T
Cuts at: 3079 3079
Size: 4691
AhdI GACnn_n'nnGTC
Cuts at: 1428 1428
Size: 4691
AleI CACnn'nnGTG
Cuts at: 964 964
Size: 4691
AlwNI CAG_nnn'CTG
Cuts at: 340 771 816 1384 1996 3563 340
Size: 431 45 568 612 1567 1468
Fragments arranged by size:
1567 1468 612 568 431 45
ApaLI G'TGCA_C
Cuts at: 3461 3461
Size: 4691
ApoI r'AATT_y
Cuts at: 1695 4044 4300 1695
Size: 2349 256 2086
Fragments arranged by size:
2349 2086 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4691
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
AvaI C'yCGr_G
Cuts at: 881 1316 1346 1711 881
Size: 435 30 365 3861
Fragments arranged by size:
3861 435 365 30
BamHI G'GATC_C
Cuts at: 132 267 1472 1716 132
Size: 135 1205 244 3107
Fragments arranged by size:
3107 1205 244 135
BanI G'GyrC_C
Cuts at: 162 822 1707 2121 2369 2404 162
Size: 660 885 414 248 35 2449
Fragments arranged by size:
2449 885 660 414 248 35
BanII G_rGCy'C
Cuts at: 607 806 1091 1515 1565 1705 2735 607
Size: 199 285 424 50 140 1030 2563
Fragments arranged by size:
2563 1030 424 285 199 140 50
BbeI G_GCGC'C
Cuts at: 166 2125 2373 166
Size: 1959 248 2484
Fragments arranged by size:
2484 1959 248
BbsI GAAGACnn'nnnn_
Cuts at: 398 827 982 398
Size: 429 155 4107
Fragments arranged by size:
4107 429 155
BbvCI CC'TCA_GC
Cuts at: 397 397
Size: 4691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 486 520 486
Size: 34 4657
BciVI GTATCCnnnnn_n'
Cuts at: 1450 1519 2589 3350 4691 1450
Size: 69 1070 761 1341 1450
Fragments arranged by size:
1450 1341 1070 761 69
BclI T'GATC_A
Cuts at: 2211 2211
Size: 4691
BglI GCCn_nnn'nGGC
Cuts at: 1015 1015
Size: 4691
BglII A'GATC_T
Cuts at: 2206 4243 2206
Size: 2037 2654
BlpI GC'TnA_GC
Cuts at: 1803 1803
Size: 4691
Bme1580I G_kGCm'C
Cuts at: 827 2316 2409 3465 827
Size: 1489 93 1056 2053
Fragments arranged by size:
2053 1489 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1903 1984 2319 4463 1903
Size: 81 335 2144 2131
Fragments arranged by size:
2144 2131 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 354 3058 354
Size: 2704 1987
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 544 919 1537 181
Size: 363 375 618 3335
Fragments arranged by size:
3335 618 375 363
Bpu10I CC'TnA_GC
Cuts at: 397 397
Size: 4691
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 877 1867 2746 3238 3536 105
Size: 772 990 879 492 298 1260
Fragments arranged by size:
1260 990 879 772 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 622 1241 1278 4395 622
Size: 619 37 3117 918
Fragments arranged by size:
3117 918 619 37
BsaAI yAC'GTr
Cuts at: 261 2044 261
Size: 1783 2908
BsaBI GATnn'nnATC
Cuts at: 6 1453 2205 4242 6
Size: 1447 752 2037 455
Fragments arranged by size:
2037 1447 752 455
BsaHI Gr'CG_yC
Cuts at: 163 1424 2122 2370 3072 4568 163
Size: 1261 698 248 702 1496 286
Fragments arranged by size:
1496 1261 702 698 286 248
BsaWI w'CCGG_w
Cuts at: 707 1529 1599 1880 2401 3079 3353 3500 3911
Size: 822 70 281 521 678 274 147 411
Cuts at: 3911 707
Size: 1487
Fragments arranged by size:
1487 822 678 521 411 281 274 147
70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 674 704 1387 1417 674
Size: 30 683 30 3948
Fragments arranged by size:
3948 683 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 547 1573 1618 169
Size: 378 1026 45 3242
Fragments arranged by size:
3242 1026 378 45
BseYI C'CCAG_C
Cuts at: 600 916 2116 2874 3451 3947 600
Size: 316 1200 758 577 496 1344
Fragments arranged by size:
1344 1200 758 577 496 316
BsiEI CG_ry'CG
Cuts at: 104 1470 1612 2233 2279 3487 104
Size: 1366 142 621 46 1208 1308
Fragments arranged by size:
1366 1308 1208 621 142 46
BsiHKAI G_wGCw'C
Cuts at: 1515 1565 1705 2483 2673 3465 1515
Size: 50 140 778 190 792 2741
Fragments arranged by size:
2741 792 778 190 140 50
BsmBI CGTCTCn'nnnn_
Cuts at: 143 143
Size: 4691
Bsp1286I G_dGCh'C
Cuts at: 607 806 827 1091 1515 1565 1705 2316 2409
Size: 199 21 264 424 50 140 611 93
Cuts at: 2409 2483 2673 2735 3465 607
Size: 74 190 62 730 1833
Fragments arranged by size:
1833 730 611 424 264 199 190 140
93 74 62 50 21
BspEI T'CCGG_A
Cuts at: 707 1599 3911 707
Size: 892 2312 1487
Fragments arranged by size:
2312 1487 892
BspHI T'CATG_A
Cuts at: 611 1574 3867 611
Size: 963 2293 1435
Fragments arranged by size:
2293 1435 963
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1741 1955 2257 2638 1741
Size: 214 302 381 3794
Fragments arranged by size:
3794 381 302 214
BsrBI CCG'CTC
Cuts at: 50 159 1608 2983 3037 4521 4686 50
Size: 109 1449 1375 54 1484 165 55
Fragments arranged by size:
1484 1449 1375 165 109 55 54
BsrDI GCAATG_nn'
Cuts at: 1168 2603 1168
Size: 1435 3256
BsrFI r'CCGG_y
Cuts at: 2689 2870 3079 2689
Size: 181 209 4301
Fragments arranged by size:
4301 209 181
BssSI C'ACGA_G
Cuts at: 2962 3320 2962
Size: 358 4333
BstAPI GCAn_nnn'nTGC
Cuts at: 1170 2068 1170
Size: 898 3793
BstBI TT'CG_AA
Cuts at: 1619 3052 1619
Size: 1433 3258
BstEII G'GTnAC_C
Cuts at: 1077 1077
Size: 4691
BstXI CCAn_nnnn'nTGG
Cuts at: 1269 1269
Size: 4691
BstYI r'GATC_y
Cuts at: 1 132 267 704 875 1472 1716 2183 2206
Size: 131 135 437 171 597 244 467 23
Cuts at: 2206 2541 2787 3799 3885 3897 4243 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 597 467 449 437 346 335 246
244 171 135 131 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4691
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2641 2822 2985 2641
Size: 181 163 4347
Fragments arranged by size:
4347 181 163
BtsI GCAGTG_nn'
Cuts at: 977 1934 977
Size: 957 3734
DraI TTT'AAA
Cuts at: 1690 1690
Size: 4691
DrdI GACnn_nn'nnGTC
Cuts at: 1057 2397 3255 1057
Size: 1340 858 2493
Fragments arranged by size:
2493 1340 858
EaeI y'GGCC_r
Cuts at: 467 941 1115 1467 1609 1655 2276 2450 2841
Size: 474 174 352 142 46 621 174 391
Cuts at: 2841 2868 4213 467
Size: 27 1345 945
Fragments arranged by size:
1345 945 621 474 391 352 174 174
142 46 27
EagI C'GGCC_G
Cuts at: 1467 1609 2276 1467
Size: 142 667 3882
Fragments arranged by size:
3882 667 142
EarI CTCTTCn'nnn_
Cuts at: 2714 2924 2714
Size: 210 4481
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2899 3209 3355 4501 2899
Size: 310 146 1146 3089
Fragments arranged by size:
3089 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1513 1563 1703 1513
Size: 50 140 4501
Fragments arranged by size:
4501 140 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 544 738 919 1406 1520 1537 2516 2948
Size: 363 194 181 487 114 17 979 432
Cuts at: 2948 3695 3963 181
Size: 747 268 909
Fragments arranged by size:
979 909 747 487 432 363 268 194
181 114 17
EcoNI CCTnn'n_nnAGG
Cuts at: 1018 1481 1018
Size: 463 4228
EcoO109I rG'GnC_Cy
Cuts at: 292 1830 292
Size: 1538 3153
EcoRI G'AATT_C
Cuts at: 1695 1695
Size: 4691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 665 2710 665
Size: 2045 2646
FspI TGC'GCA
Cuts at: 2472 2472
Size: 4691
HaeII r_GCGC'y
Cuts at: 120 166 239 576 2125 2373 3395 4374 120
Size: 46 73 337 1549 248 1022 979 437
Fragments arranged by size:
1549 1022 979 437 337 248 73 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 259 291 1023 1055 1414 1446 1497 1529 2533
Size: 32 732 32 359 32 51 32 1004
Cuts at: 2533 2563 2565 2595 259
Size: 30 2 30 2355
Fragments arranged by size:
2355 1004 732 359 51 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 516 1730 516
Size: 1214 3477
HindIII A'AGCT_T
Cuts at: 1125 1125
Size: 4691
KasI G'GCGC_C
Cuts at: 162 2121 2369 162
Size: 1959 248 2484
Fragments arranged by size:
2484 1959 248
KpnI G_GTAC'C
Cuts at: 1711 1711
Size: 4691
MluI A'CGCG_T
Cuts at: 4124 4124
Size: 4691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 183 3362 3546 183
Size: 3179 184 1328
Fragments arranged by size:
3179 1328 184
MscI TGG'CCA
Cuts at: 469 943 1117 2452 469
Size: 474 174 1335 2708
Fragments arranged by size:
2708 1335 474 174
MslI CAynn'nnrTG
Cuts at: 352 964 1000 1576 2680 2807 352
Size: 612 36 576 1104 127 2236
Fragments arranged by size:
2236 1104 612 576 127 36
MspA1I CmG'CkG
Cuts at: 1802 2116 2476 3489 3734 1802
Size: 314 360 1013 245 2759
Fragments arranged by size:
2759 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2872 2872
Size: 4691
NarI GG'CG_CC
Cuts at: 163 2122 2370 163
Size: 1959 248 2484
Fragments arranged by size:
2484 1959 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4691
NgoMIV G'CCGG_C
Cuts at: 2870 2870
Size: 4691
NspI r_CATG'y
Cuts at: 600 834 999 1744 2244 3151 600
Size: 234 165 745 500 907 2140
Fragments arranged by size:
2140 907 745 500 234 165
PasI CC'CwG_GG
Cuts at: 1483 1483
Size: 4691
PciI A'CATG_T
Cuts at: 3147 3147
Size: 4691
PflMI CCAn_nnn'nTGG
Cuts at: 423 582 1373 423
Size: 159 791 3741
Fragments arranged by size:
3741 791 159
PfoI T'CCnGG_A
Cuts at: 617 617
Size: 4691
PmeI GTTT'AAAC
Cuts at: 1690 1690
Size: 4691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4659
PstI C_TGCA'G
Cuts at: 767 1046 1738 2423 767
Size: 279 692 685 3035
Fragments arranged by size:
3035 692 685 279
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4691
PvuII CAG'CTG
Cuts at: 2116 2476 2116
Size: 360 4331
RsrII CG'GwC_CG
Cuts at: 2886 2886
Size: 4691
SacI G_AGCT'C
Cuts at: 1515 1565 1705 1515
Size: 50 140 4501
Fragments arranged by size:
4501 140 50
SalI G'TCGA_C
Cuts at: 1728 1728
Size: 4691
SapI GCTCTTCn'nnn_
Cuts at: 2714 2924 2714
Size: 210 4481
SbfI CC_TGCA'GG
Cuts at: 1738 1738
Size: 4691
ScaI AGT'ACT
Cuts at: 4208 4208
Size: 4691
SfcI C'TryA_G
Cuts at: 33 763 1042 1734 2419 3412 3603 33
Size: 730 279 692 685 993 191 1121
Fragments arranged by size:
1121 993 730 692 685 279 191
SfoI GGC'GCC
Cuts at: 164 2123 2371 164
Size: 1959 248 2484
Fragments arranged by size:
2484 1959 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
SmaI CCC'GGG
Cuts at: 1318 1713 1318
Size: 395 4296
SmlI C'TyrA_G
Cuts at: 120 892 1846 2761 3253 3515 120
Size: 772 954 915 492 262 1296
Fragments arranged by size:
1296 954 915 772 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4691
SphI G_CATG'C
Cuts at: 1744 2244 1744
Size: 500 4191
StuI AGG'CCT
Cuts at: 1009 1009
Size: 4691
StyI C'CwwG_G
Cuts at: 107 853 962 1082 1825 2179 107
Size: 746 109 120 743 354 2619
Fragments arranged by size:
2619 746 743 354 120 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2274 2407 2866 3075 2274
Size: 133 459 209 3890
Fragments arranged by size:
3890 459 209 133
TatI w'GTAC_w
Cuts at: 1337 4206 1337
Size: 2869 1822
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 674 706 4208 4240 674
Size: 32 3502 32 1125
Fragments arranged by size:
3502 1125 32 32
Tth111I GACn'n_nGTC
Cuts at: 2488 2488
Size: 4691
XbaI T'CTAG_A
Cuts at: 1722 1722
Size: 4691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2186 2186
Size: 4691
XmaI C'CCGG_G
Cuts at: 1316 1711 1316
Size: 395 4296
ZraI GAC'GTC
Cuts at: 1425 1425
Size: 4691
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI