Restriction map for
pF1KE2320
(Circular) (Six-base) MAPSORT of: pF1KE2320.seq Check: 7820 from: 1 to: 4871
pF1KE2320 4871 bp
With 182 enzymes: SgfI *
January 11, 2011 13:49 ..
AccI GT'mk_AC
Cuts at: 515 1909 515
Size: 1394 3477
Acc65I G'GTAC_C
Cuts at: 687 1887 687
Size: 1200 3671
AclI AA'CG_TT
Cuts at: 4711 4711
Size: 4871
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1150 2696 3128 3875 4143 439
Size: 711 1546 432 747 268 1167
Fragments arranged by size:
1546 1167 747 711 432 268
AflIII A'CryG_T
Cuts at: 359 2223 3327 4304 359
Size: 1864 1104 977 926
Fragments arranged by size:
1864 1104 977 926
AgeI A'CCGG_T
Cuts at: 3259 3259
Size: 4871
AleI CACnn'nnGTG
Cuts at: 541 541
Size: 4871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 465 1369 465
Size: 904 3967
AlwNI CAG_nnn'CTG
Cuts at: 1533 2176 3743 1533
Size: 643 1567 2661
Fragments arranged by size:
2661 1567 643
ApaLI G'TGCA_C
Cuts at: 3641 3641
Size: 4871
ApoI r'AATT_y
Cuts at: 580 718 1651 1875 4224 4480 580
Size: 138 933 224 2349 256 971
Fragments arranged by size:
2349 971 933 256 224 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 4871
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
AvaI C'yCGr_G
Cuts at: 1724 1787 1891 1724
Size: 63 104 4704
Fragments arranged by size:
4704 104 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1435 1468 1435
Size: 33 4838
BamHI G'GATC_C
Cuts at: 1896 1896
Size: 4871
BanI G'GyrC_C
Cuts at: 258 554 687 1345 1887 2301 2549 2584 258
Size: 296 133 658 542 414 248 35 2545
Fragments arranged by size:
2545 658 542 414 296 248 133 35
BanII G_rGCy'C
Cuts at: 173 1714 1885 2915 173
Size: 1541 171 1030 2129
Fragments arranged by size:
2129 1541 1030 171
BbeI G_GCGC'C
Cuts at: 262 2305 2553 262
Size: 2043 248 2580
Fragments arranged by size:
2580 2043 248
BbsI GAAGACnn'nnnn_
Cuts at: 678 678
Size: 4871
BbvCI CC'TCA_GC
Cuts at: 1109 1277 1109
Size: 168 4703
BciVI GTATCCnnnnn_n'
Cuts at: 988 1463 2769 3530 4871 988
Size: 475 1306 761 1341 988
Fragments arranged by size:
1341 1306 988 761 475
BclI T'GATC_A
Cuts at: 2391 2391
Size: 4871
BglI GCCn_nnn'nGGC
Cuts at: 391 391
Size: 4871
BglII A'GATC_T
Cuts at: 2386 4423 2386
Size: 2037 2834
BlpI GC'TnA_GC
Cuts at: 527 571 1983 527
Size: 44 1412 3415
Fragments arranged by size:
3415 1412 44
Bme1580I G_kGCm'C
Cuts at: 559 1348 2496 2589 3645 559
Size: 789 1148 93 1056 1785
Fragments arranged by size:
1785 1148 1056 789 93
BmgBI CAC'GTC
Cuts at: 360 534 360
Size: 174 4697
BmrI ACTGGGnnnn_n'
Cuts at: 639 2083 2164 2499 4643 639
Size: 1444 81 335 2144 867
Fragments arranged by size:
2144 1444 867 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1578 3238 1578
Size: 1660 3211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1385 1698 1385
Size: 313 4558
Bpu10I CC'TnA_GC
Cuts at: 1109 1277 1595 1109
Size: 168 318 4385
Fragments arranged by size:
4385 318 168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2047 2926 3418 3716 2047
Size: 879 492 298 3202
Fragments arranged by size:
3202 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1714 4575 1714
Size: 2861 2010
BsaAI yAC'GTr
Cuts at: 2224 2224
Size: 4871
BsaBI GATnn'nnATC
Cuts at: 6 2385 4422 6
Size: 2379 2037 455
Fragments arranged by size:
2379 2037 455
BsaHI Gr'CG_yC
Cuts at: 259 2302 2550 3252 4748 259
Size: 2043 248 702 1496 382
Fragments arranged by size:
2043 1496 702 382 248
BsaWI w'CCGG_w
Cuts at: 2060 2581 3259 3533 3680 4091 2060
Size: 521 678 274 147 411 2840
Fragments arranged by size:
2840 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 465 495 1639 1669 465
Size: 30 1144 30 3667
Fragments arranged by size:
3667 1144 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 198 473 1647 1870 198
Size: 275 1174 223 3199
Fragments arranged by size:
3199 1174 275 223
BseYI C'CCAG_C
Cuts at: 280 2296 3054 3631 4127 280
Size: 2016 758 577 496 1024
Fragments arranged by size:
2016 1024 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1419 1419
Size: 4871
BsiEI CG_ry'CG
Cuts at: 104 2413 2459 3667 104
Size: 2309 46 1208 1308
Fragments arranged by size:
2309 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 382 533 1885 2663 2853 3645 382
Size: 151 1352 778 190 792 1608
Fragments arranged by size:
1608 1352 792 778 190 151
Bsp1286I G_dGCh'C
Cuts at: 173 382 533 559 1348 1714 1885 2496 2589
Size: 209 151 26 789 366 171 611 93
Cuts at: 2589 2663 2853 2915 3645 173
Size: 74 190 62 730 1399
Fragments arranged by size:
1399 789 730 611 366 209 190 171
151 93 74 62 26
BspEI T'CCGG_A
Cuts at: 4091 4091
Size: 4871
BspHI T'CATG_A
Cuts at: 4047 4047
Size: 4871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1921 2135 2437 2818 1921
Size: 214 302 381 3974
Fragments arranged by size:
3974 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3163 3217 4701 4866 50
Size: 3113 54 1484 165 55
Fragments arranged by size:
3113 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2783 2783
Size: 4871
BsrFI r'CCGG_y
Cuts at: 1637 2869 3050 3259 1637
Size: 1232 181 209 3249
Fragments arranged by size:
3249 1232 209 181
BsrGI T'GTAC_A
Cuts at: 734 734
Size: 4871
BssSI C'ACGA_G
Cuts at: 3142 3500 3142
Size: 358 4513
BstAPI GCAn_nnn'nTGC
Cuts at: 2248 2248
Size: 4871
BstBI TT'CG_AA
Cuts at: 3232 3232
Size: 4871
BstYI r'GATC_y
Cuts at: 1 657 854 1006 1896 2363 2386 2721 2967
Size: 656 197 152 890 467 23 335 246
Cuts at: 2967 3979 4065 4077 4423 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 890 656 467 449 346 335 246
197 152 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 485 485
Size: 4871
BtgI C'CryG_G
Cuts at: 1565 1565
Size: 4871
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2821 3002 3165 2821
Size: 181 163 4527
Fragments arranged by size:
4527 181 163
BtsI GCAGTG_nn'
Cuts at: 1431 1695 2114 1431
Size: 264 419 4188
Fragments arranged by size:
4188 419 264
DraI TTT'AAA
Cuts at: 1870 1870
Size: 4871
DrdI GACnn_nn'nnGTC
Cuts at: 2577 3435 2577
Size: 858 4013
EaeI y'GGCC_r
Cuts at: 392 2456 2630 3021 3048 4393 392
Size: 2064 174 391 27 1345 870
Fragments arranged by size:
2064 1345 870 391 174 27
EagI C'GGCC_G
Cuts at: 2456 2456
Size: 4871
EarI CTCTTCn'nnn_
Cuts at: 867 2894 3104 867
Size: 2027 210 2634
Fragments arranged by size:
2634 2027 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 901 1753 3079 3389 3535 4681 901
Size: 852 1326 310 146 1146 1091
Fragments arranged by size:
1326 1146 1091 852 310 146
EcoICRI GAG'CTC
Cuts at: 1883 1883
Size: 4871
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1150 1385 1698 2696 3128 3875 4143 439
Size: 711 235 313 998 432 747 268 1167
Fragments arranged by size:
1167 998 747 711 432 313 268 235
EcoO109I rG'GnC_Cy
Cuts at: 2010 2010
Size: 4871
EcoRI G'AATT_C
Cuts at: 580 718 1651 1875 580
Size: 138 933 224 3576
Fragments arranged by size:
3576 933 224 138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2890 2890
Size: 4871
FspI TGC'GCA
Cuts at: 2652 2652
Size: 4871
HaeII r_GCGC'y
Cuts at: 262 954 2305 2553 3575 4554 262
Size: 692 1351 248 1022 979 579
Fragments arranged by size:
1351 1022 979 692 579 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 465 482 497 514 588 620 1598
Size: 32 147 17 15 17 74 32 978
Cuts at: 1598 1630 1745 1777 2713 2743 2745 2775 286
Size: 32 115 32 936 30 2 30 2382
Fragments arranged by size:
2382 978 936 147 115 74 32 32
32 32 30 30 17 17 15 2
HincII GTy'rAC
Cuts at: 1910 1910
Size: 4871
KasI G'GCGC_C
Cuts at: 258 2301 2549 258
Size: 2043 248 2580
Fragments arranged by size:
2580 2043 248
KpnI G_GTAC'C
Cuts at: 691 1891 691
Size: 1200 3671
MfeI C'AATT_G
Cuts at: 1118 1118
Size: 4871
MluI A'CGCG_T
Cuts at: 4304 4304
Size: 4871
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 350 744 3542 3726 350
Size: 394 2798 184 1495
Fragments arranged by size:
2798 1495 394 184
MscI TGG'CCA
Cuts at: 2632 2632
Size: 4871
MslI CAynn'nnrTG
Cuts at: 541 1336 1351 1357 2860 2987 541
Size: 795 15 6 1503 127 2425
Fragments arranged by size:
2425 1503 795 127 15 6
MspA1I CmG'CkG
Cuts at: 1982 2296 2656 3669 3914 1982
Size: 314 360 1013 245 2939
Fragments arranged by size:
2939 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3052 3052
Size: 4871
NarI GG'CG_CC
Cuts at: 259 2302 2550 259
Size: 2043 248 2580
Fragments arranged by size:
2580 2043 248
NcoI C'CATG_G
Cuts at: 1565 1565
Size: 4871
NdeI CA'TA_TG
Cuts at: 1200 1200
Size: 4871
NgoMIV G'CCGG_C
Cuts at: 3050 3050
Size: 4871
NspI r_CATG'y
Cuts at: 452 1356 1612 1924 2424 3331 452
Size: 904 256 312 500 907 1992
Fragments arranged by size:
1992 907 904 500 312 256
PasI CC'CwG_GG
Cuts at: 1527 1527
Size: 4871
PciI A'CATG_T
Cuts at: 3327 3327
Size: 4871
PflMI CCAn_nnn'nTGG
Cuts at: 1363 1363
Size: 4871
PfoI T'CCnGG_A
Cuts at: 1628 1628
Size: 4871
PmeI GTTT'AAAC
Cuts at: 1870 1870
Size: 4871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 193 225 4010 4042 193
Size: 32 3785 32 1022
Fragments arranged by size:
3785 1022 32 32
PstI C_TGCA'G
Cuts at: 1918 2603 1918
Size: 685 4186
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4871
PvuII CAG'CTG
Cuts at: 2296 2656 2296
Size: 360 4511
RsrII CG'GwC_CG
Cuts at: 3066 3066
Size: 4871
SacI G_AGCT'C
Cuts at: 1885 1885
Size: 4871
SalI G'TCGA_C
Cuts at: 1908 1908
Size: 4871
SapI GCTCTTCn'nnn_
Cuts at: 2894 3104 2894
Size: 210 4661
SbfI CC_TGCA'GG
Cuts at: 1918 1918
Size: 4871
ScaI AGT'ACT
Cuts at: 4388 4388
Size: 4871
SfcI C'TryA_G
Cuts at: 33 1697 1914 2599 3592 3783 33
Size: 1664 217 685 993 191 1121
Fragments arranged by size:
1664 1121 993 685 217 191
SfoI GGC'GCC
Cuts at: 260 2303 2551 260
Size: 2043 248 2580
Fragments arranged by size:
2580 2043 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
SmaI CCC'GGG
Cuts at: 1726 1893 1726
Size: 167 4704
SmlI C'TyrA_G
Cuts at: 2026 2941 3433 3695 2026
Size: 915 492 262 3202
Fragments arranged by size:
3202 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4871
SphI G_CATG'C
Cuts at: 1924 2424 1924
Size: 500 4371
StyI C'CwwG_G
Cuts at: 1565 2005 2359 1565
Size: 440 354 4077
Fragments arranged by size:
4077 440 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1363 1405 2454 2587 3046 3255 1363
Size: 42 1049 133 459 209 2979
Fragments arranged by size:
2979 1049 459 209 133 42
TatI w'GTAC_w
Cuts at: 155 734 925 4386 155
Size: 579 191 3461 640
Fragments arranged by size:
3461 640 579 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 302 334 962 994 1639 1671 4388 4420 302
Size: 32 628 32 645 32 2717 32 753
Fragments arranged by size:
2717 753 645 628 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2668 2668
Size: 4871
XbaI T'CTAG_A
Cuts at: 1902 1902
Size: 4871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1572 2366 114
Size: 1458 794 2619
Fragments arranged by size:
2619 1458 794
XmaI C'CCGG_G
Cuts at: 1724 1891 1724
Size: 167 4704
XmnI GAAnn'nnTTC
Cuts at: 1804 1804
Size: 4871
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BcgI BfrBI BmtI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI