Restriction map for
pF1KE2310
(Circular) (Six-base) MAPSORT of: pF1KE2310.seq Check: 5293 from: 1 to: 7037
pF1KE2310 7037 bp
With 182 enzymes: SgfI *
January 5, 2011 13:33 ..
AccI GT'mk_AC
Cuts at: 554 908 2781 2997 4075 554
Size: 354 1873 216 1078 3516
Fragments arranged by size:
3516 1873 1078 354 216
Acc65I G'GTAC_C
Cuts at: 2319 4053 2319
Size: 1734 5303
AclI AA'CG_TT
Cuts at: 2919 6877 2919
Size: 3958 3079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1332 1477 1524 1622 1668 2281 2345 3793 3850
Size: 145 47 98 46 613 64 1448 57
Cuts at: 3850 3997 4862 5294 6041 6309 1332
Size: 147 865 432 747 268 2060
Fragments arranged by size:
2060 1448 865 747 613 432 268 147
145 98 64 57 47 46
AflIII A'CryG_T
Cuts at: 365 4389 5493 6470 365
Size: 4024 1104 977 932
Fragments arranged by size:
4024 1104 977 932
AgeI A'CCGG_T
Cuts at: 5425 5425
Size: 7037
AhdI GACnn_n'nnGTC
Cuts at: 130 781 2378 130
Size: 651 1597 4789
Fragments arranged by size:
4789 1597 651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4009 4009
Size: 7037
AlwNI CAG_nnn'CTG
Cuts at: 791 1455 3988 4342 5909 791
Size: 664 2533 354 1567 1919
Fragments arranged by size:
2533 1919 1567 664 354
ApaLI G'TGCA_C
Cuts at: 5807 5807
Size: 7037
ApoI r'AATT_y
Cuts at: 408 565 858 1171 1200 1732 2794 2876 3004
Size: 157 293 313 29 532 1062 82 128
Cuts at: 3004 4041 6390 6646 408
Size: 1037 2349 256 799
Fragments arranged by size:
2349 1062 1037 799 532 313 293 256
157 128 82 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 7037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7037
AvaI C'yCGr_G
Cuts at: 3041 4057 3041
Size: 1016 6021
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 226 259 226
Size: 33 7004
BamHI G'GATC_C
Cuts at: 3926 4062 3926
Size: 136 6901
BanI G'GyrC_C
Cuts at: 2319 3433 3453 4053 4467 4715 4750 2319
Size: 1114 20 600 414 248 35 4606
Fragments arranged by size:
4606 1114 600 414 248 35 20
BanII G_rGCy'C
Cuts at: 220 2090 2476 3357 3529 4051 5081 220
Size: 1870 386 881 172 522 1030 2176
Fragments arranged by size:
2176 1870 1030 881 522 386 172
BbeI G_GCGC'C
Cuts at: 4471 4719 4471
Size: 248 6789
BbvCI CC'TCA_GC
Cuts at: 2927 3164 2927
Size: 237 6800
BciVI GTATCCnnnnn_n'
Cuts at: 4935 5696 7037 4935
Size: 761 1341 4935
Fragments arranged by size:
4935 1341 761
BclI T'GATC_A
Cuts at: 2417 4557 2417
Size: 2140 4897
BfrBI ATG'CAT
Cuts at: 3679 3679
Size: 7037
BglII A'GATC_T
Cuts at: 4552 6589 4552
Size: 2037 5000
BlpI GC'TnA_GC
Cuts at: 4149 4149
Size: 7037
Bme1580I G_kGCm'C
Cuts at: 3347 4662 4755 5811 3347
Size: 1315 93 1056 4573
Fragments arranged by size:
4573 1315 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1773 2201 3089 4249 4330 4665 6809 1773
Size: 428 888 1160 81 335 2144 2001
Fragments arranged by size:
2144 2001 1160 888 428 335 81
BmtI G_CTAG'C
Cuts at: 2701 2701
Size: 7037
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1770 5404 1770
Size: 3634 3403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 424 833 952 2093 2794 316
Size: 108 409 119 1141 701 4559
Fragments arranged by size:
4559 1141 701 409 119 108
Bpu10I CC'TnA_GC
Cuts at: 822 2283 2591 2927 3164 822
Size: 1461 308 336 237 4695
Fragments arranged by size:
4695 1461 336 308 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2184 4213 5092 5584 5882 1402
Size: 782 2029 879 492 298 2557
Fragments arranged by size:
2557 2029 879 782 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3038 3635 6741 3038
Size: 597 3106 3334
Fragments arranged by size:
3334 3106 597
BsaAI yAC'GTr
Cuts at: 366 4390 366
Size: 4024 3013
BsaBI GATnn'nnATC
Cuts at: 6 1937 4551 6588 6
Size: 1931 2614 2037 455
Fragments arranged by size:
2614 2037 1931 455
BsaHI Gr'CG_yC
Cuts at: 4468 4716 5418 6914 4468
Size: 248 702 1496 4591
Fragments arranged by size:
4591 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2450 4016 4226 4747 5425 5699 5846 6257 2450
Size: 1566 210 521 678 274 147 411 3230
Fragments arranged by size:
3230 1566 678 521 411 274 210 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 536 566 866 896 2321 2351 3326 3356 536
Size: 30 300 30 1425 30 975 30 4217
Fragments arranged by size:
4217 1425 975 300 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1795 3181 3254 3548 3622 1795
Size: 1386 73 294 74 5210
Fragments arranged by size:
5210 1386 294 74 73
BseYI C'CCAG_C
Cuts at: 172 698 2457 4462 5220 5797 6293 172
Size: 526 1759 2005 758 577 496 916
Fragments arranged by size:
2005 1759 916 758 577 526 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 767 1953 2925 215
Size: 552 1186 972 4327
Fragments arranged by size:
4327 1186 972 552
BsiEI CG_ry'CG
Cuts at: 104 4579 4625 5833 104
Size: 4475 46 1208 1308
Fragments arranged by size:
4475 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 220 317 938 1849 2540 3862 4051 4829 5019
Size: 97 621 911 691 1322 189 778 190
Cuts at: 5019 5811 220
Size: 792 1446
Fragments arranged by size:
1446 1322 911 792 778 691 621 190
189 97
BsmI GAATG_Cn'
Cuts at: 967 967
Size: 7037
Bsp1286I G_dGCh'C
Cuts at: 220 317 938 1849 2090 2476 2540 3347 3357
Size: 97 621 911 241 386 64 807 10
Cuts at: 3357 3529 3862 4051 4662 4755 4829 5019 5081
Size: 172 333 189 611 93 74 190 62
Cuts at: 5081 5811 220
Size: 730 1446
Fragments arranged by size:
1446 911 807 730 621 611 386 333
241 190 189 172 97 93 74 64
62 10
BspEI T'CCGG_A
Cuts at: 6257 6257
Size: 7037
BspHI T'CATG_A
Cuts at: 1998 6213 1998
Size: 4215 2822
BspMI ACCTGCnnnn'nnnn_
Cuts at: 404 3890 4087 4301 4603 4984 404
Size: 3486 197 214 302 381 2457
Fragments arranged by size:
3486 2457 381 302 214 197
BsrBI CCG'CTC
Cuts at: 50 5329 5383 6867 7032 50
Size: 5279 54 1484 165 55
Fragments arranged by size:
5279 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 717 4949 717
Size: 4232 2805
BsrFI r'CCGG_y
Cuts at: 114 5035 5216 5425 114
Size: 4921 181 209 1726
Fragments arranged by size:
4921 1726 209 181
BsrGI T'GTAC_A
Cuts at: 1837 1837
Size: 7037
BssSI C'ACGA_G
Cuts at: 148 2533 3530 5308 5666 148
Size: 2385 997 1778 358 1519
Fragments arranged by size:
2385 1778 1519 997 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3394 4414 3394
Size: 1020 6017
BstBI TT'CG_AA
Cuts at: 5398 5398
Size: 7037
BstEII G'GTnAC_C
Cuts at: 1368 1368
Size: 7037
BstXI CCAn_nnnn'nTGG
Cuts at: 1764 2487 2709 2762 3753 1764
Size: 723 222 53 991 5048
Fragments arranged by size:
5048 991 723 222 53
BstYI r'GATC_y
Cuts at: 1 1697 3926 4062 4529 4552 4887 5133 6145
Size: 1696 2229 136 467 23 335 246 1012
Cuts at: 6145 6231 6243 6589 1
Size: 86 12 346 449
Fragments arranged by size:
2229 1696 1012 467 449 346 335 246
136 86 23 12
BstZ17I GTA'TAC
Cuts at: 2782 2782
Size: 7037
Bsu36I CC'TnA_GG
Cuts at: 830 3182 830
Size: 2352 4685
BtgI C'CryG_G
Cuts at: 616 1815 2017 2508 616
Size: 1199 202 491 5145
Fragments arranged by size:
5145 1199 491 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4987 5168 5331 4987
Size: 181 163 6693
Fragments arranged by size:
6693 181 163
BtsI GCAGTG_nn'
Cuts at: 4280 4280
Size: 7037
ClaI AT'CG_AT
Cuts at: 447 447
Size: 7037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 852 887 1856 1891 3492 3527 852
Size: 35 969 35 1601 35 4362
Fragments arranged by size:
4362 1601 969 35 35 35
DraI TTT'AAA
Cuts at: 470 1857 4036 470
Size: 1387 2179 3471
Fragments arranged by size:
3471 2179 1387
DraIII CAC_nnn'GTG
Cuts at: 3394 3394
Size: 7037
DrdI GACnn_nn'nnGTC
Cuts at: 639 4743 5601 639
Size: 4104 858 2075
Fragments arranged by size:
4104 2075 858
EaeI y'GGCC_r
Cuts at: 690 1327 4622 4796 5187 5214 6559 690
Size: 637 3295 174 391 27 1345 1168
Fragments arranged by size:
3295 1345 1168 637 391 174 27
EagI C'GGCC_G
Cuts at: 4622 4622
Size: 7037
EarI CTCTTCn'nnn_
Cuts at: 1377 1543 2095 2134 2257 3131 5060 5270 1377
Size: 166 552 39 123 874 1929 210 3144
Fragments arranged by size:
3144 1929 874 552 210 166 123 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3939 5245 5555 5701 6847 3939
Size: 1306 310 146 1146 4129
Fragments arranged by size:
4129 1306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 218 4049 218
Size: 3831 3206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 424 833 952 1332 1477 1524 1622 1668
Size: 108 409 119 380 145 47 98 46
Cuts at: 1668 2093 2281 2345 2794 3793 3850 3997 4862
Size: 425 188 64 449 999 57 147 865
Cuts at: 4862 5294 6041 6309 316
Size: 432 747 268 1044
Fragments arranged by size:
1044 999 865 747 449 432 425 409
380 268 188 147 145 119 108 98
64 57 47 46
EcoO109I rG'GnC_Cy
Cuts at: 324 703 826 1233 1648 1825 4176 324
Size: 379 123 407 415 177 2351 3185
Fragments arranged by size:
3185 2351 415 407 379 177 123
EcoRI G'AATT_C
Cuts at: 2876 4041 2876
Size: 1165 5872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 5056 2251
Size: 2805 4232
FspI TGC'GCA
Cuts at: 256 4818 256
Size: 4562 2475
HaeII r_GCGC'y
Cuts at: 4471 4719 5741 6720 4471
Size: 248 1022 979 4788
Fragments arranged by size:
4788 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 116 148 169 201 767 799 1125 1157 1682
Size: 32 21 32 566 32 326 32 525
Cuts at: 1682 1714 2364 2396 4879 4909 4911 4941 116
Size: 32 650 32 2483 30 2 30 2212
Fragments arranged by size:
2483 2212 650 566 525 326 32 32
32 32 32 32 30 30 21 2
HincII GTy'rAC
Cuts at: 644 4076 644
Size: 3432 3605
HindIII A'AGCT_T
Cuts at: 1103 3088 1103
Size: 1985 5052
KasI G'GCGC_C
Cuts at: 4467 4715 4467
Size: 248 6789
KpnI G_GTAC'C
Cuts at: 2323 4057 2323
Size: 1734 5303
MfeI C'AATT_G
Cuts at: 1247 1247
Size: 7037
MluI A'CGCG_T
Cuts at: 6470 6470
Size: 7037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 650 940 2501 2905 2956 5708 5892 650
Size: 290 1561 404 51 2752 184 1795
Fragments arranged by size:
2752 1795 1561 404 290 184 51
MscI TGG'CCA
Cuts at: 1329 4798 1329
Size: 3469 3568
MslI CAynn'nnrTG
Cuts at: 1762 2807 3280 5026 5153 1762
Size: 1045 473 1746 127 3646
Fragments arranged by size:
3646 1746 1045 473 127
MspA1I CmG'CkG
Cuts at: 2071 2487 4148 4462 4822 5835 6080 2071
Size: 416 1661 314 360 1013 245 3028
Fragments arranged by size:
3028 1661 1013 416 360 314 245
NaeI GCC'GGC
Cuts at: 116 5218 116
Size: 5102 1935
NarI GG'CG_CC
Cuts at: 4468 4716 4468
Size: 248 6789
NcoI C'CATG_G
Cuts at: 616 1815 2017 2508 616
Size: 1199 202 491 5145
Fragments arranged by size:
5145 1199 491 202
NdeI CA'TA_TG
Cuts at: 960 960
Size: 7037
NgoMIV G'CCGG_C
Cuts at: 114 5216 114
Size: 5102 1935
NheI G'CTAG_C
Cuts at: 2697 2697
Size: 7037
NsiI A_TGCA'T
Cuts at: 3681 3681
Size: 7037
NspI r_CATG'y
Cuts at: 881 1814 2050 4090 4590 5497 881
Size: 933 236 2040 500 907 2421
Fragments arranged by size:
2421 2040 933 907 500 236
PciI A'CATG_T
Cuts at: 5493 5493
Size: 7037
PflMI CCAn_nnn'nTGG
Cuts at: 593 1526 593
Size: 933 6104
PfoI T'CCnGG_A
Cuts at: 1568 1568
Size: 7037
PmeI GTTT'AAAC
Cuts at: 4036 4036
Size: 7037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6176 6208 6176
Size: 32 7005
PpuMI rG'GwC_Cy
Cuts at: 324 703 826 1233 1648 1825 324
Size: 379 123 407 415 177 5536
Fragments arranged by size:
5536 415 407 379 177 123
PshAI GACnn'nnGTC
Cuts at: 1664 1664
Size: 7037
PsiI TTA'TAA
Cuts at: 2306 3911 2306
Size: 1605 5432
PstI C_TGCA'G
Cuts at: 3887 4084 4769 3887
Size: 197 685 6155
Fragments arranged by size:
6155 685 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7037
PvuII CAG'CTG
Cuts at: 2071 2487 4462 4822 2071
Size: 416 1975 360 4286
Fragments arranged by size:
4286 1975 416 360
RsrII CG'GwC_CG
Cuts at: 5232 5232
Size: 7037
SacI G_AGCT'C
Cuts at: 220 4051 220
Size: 3831 3206
SalI G'TCGA_C
Cuts at: 4074 4074
Size: 7037
SanDI GG'GwC_CC
Cuts at: 703 1233 703
Size: 530 6507
SapI GCTCTTCn'nnn_
Cuts at: 2095 2134 5060 5270 2095
Size: 39 2926 210 3862
Fragments arranged by size:
3862 2926 210 39
SbfI CC_TGCA'GG
Cuts at: 3887 4084 3887
Size: 197 6840
ScaI AGT'ACT
Cuts at: 262 1388 3172 6554 262
Size: 1126 1784 3382 745
Fragments arranged by size:
3382 1784 1126 745
SexAI A'CCwGG_T
Cuts at: 1364 1364
Size: 7037
SfcI C'TryA_G
Cuts at: 33 474 1392 1441 3348 3883 4080 4765 5758
Size: 441 918 49 1907 535 197 685 993
Cuts at: 5758 5949 33
Size: 191 1121
Fragments arranged by size:
1907 1121 993 918 685 535 441 197
191 49
SfoI GGC'GCC
Cuts at: 4469 4717 4469
Size: 248 6789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7037
SmaI CCC'GGG
Cuts at: 4059 4059
Size: 7037
SmlI C'TyrA_G
Cuts at: 1417 2163 3041 4192 5107 5599 5861 1417
Size: 746 878 1151 915 492 262 2593
Fragments arranged by size:
2593 1151 915 878 746 492 262
SpeI A'CTAG_T
Cuts at: 67 1993 67
Size: 1926 5111
SphI G_CATG'C
Cuts at: 4090 4590 4090
Size: 500 6537
SspI AAT'ATT
Cuts at: 464 3904 464
Size: 3440 3597
StyI C'CwwG_G
Cuts at: 455 616 1815 1974 2017 2277 2384 2508 3695
Size: 161 1199 159 43 260 107 124 1187
Cuts at: 3695 3752 4171 4525 455
Size: 57 419 354 2967
Fragments arranged by size:
2967 1199 1187 419 354 260 161 159
124 107 57 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 126 1967 1987 2235 2449 2815 3496 3708 3887
Size: 1841 20 248 214 366 681 212 179
Cuts at: 3887 4620 4753 5212 5421 126
Size: 733 133 459 209 1742
Fragments arranged by size:
1841 1742 733 681 459 366 248 214
212 209 179 133 20
TatI w'GTAC_w
Cuts at: 260 685 953 1386 1594 1837 2729 3170 3890
Size: 425 268 433 208 243 892 441 720
Cuts at: 3890 6552 260
Size: 2662 745
Fragments arranged by size:
2662 892 745 720 441 433 425 268
243 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 729 761 2321 2353 2432 2464 3326 3358 3446
Size: 32 1560 32 79 32 862 32 88
Cuts at: 3446 3478 3664 3696 6554 6586 729
Size: 32 186 32 2858 32 1180
Fragments arranged by size:
2858 1560 1180 862 186 88 79 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1823 4834 1823
Size: 3011 4026
XbaI T'CTAG_A
Cuts at: 4068 4068
Size: 7037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 927 2391 4532 927
Size: 1464 2141 3432
Fragments arranged by size:
3432 2141 1464
XhoI C'TCGA_G
Cuts at: 3041 3041
Size: 7037
XmaI C'CCGG_G
Cuts at: 4057 4057
Size: 7037
XmnI GAAnn'nnTTC
Cuts at: 565 565
Size: 7037
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BbsI BcgI BglI BmgBI BsiWI BsmBI BssHII
EcoNI EcoRV FseI FspAI HpaI NotI NruI PacI
PasI PmlI PspOMI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI StuI SwaI ZraI