Restriction map for
pF1KE2305
(Circular) (Six-base) MAPSORT of: pF1KE2305.seq Check: 1197 from: 1 to: 13049
pF1KE2305 13049 bp
With 182 enzymes: SgfI *
December 13, 2010 18:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1755 4582 6028 6110 6824 1755
Size: 2827 1446 82 714 7980
Fragments arranged by size:
7980 2827 1446 714 82
AatII G_ACGT'C
Cuts at: 4037 4037
Size: 13049
AccI GT'mk_AC
Cuts at: 151 1190 2450 10087 151
Size: 1039 1260 7637 3113
Fragments arranged by size:
7637 3113 1260 1039
Acc65I G'GTAC_C
Cuts at: 5468 10065 5468
Size: 4597 8452
AclI AA'CG_TT
Cuts at: 12889 12889
Size: 13049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 1034 5322 5797 7253 8822 9160 10874 11306
Size: 426 4288 475 1456 1569 338 1714 432
Cuts at: 11306 12053 12321 608
Size: 747 268 1336
Fragments arranged by size:
4288 1714 1569 1456 1336 747 475 432
426 338 268
AfeI AGC'GCT
Cuts at: 1753 3964 5326 1753
Size: 2211 1362 9476
Fragments arranged by size:
9476 2211 1362
AflIII A'CryG_T
Cuts at: 1143 3950 4238 4478 10401 11505 12482 1143
Size: 2807 288 240 5923 1104 977 1710
Fragments arranged by size:
5923 2807 1710 1104 977 288 240
AgeI A'CCGG_T
Cuts at: 9146 11437 9146
Size: 2291 10758
AhdI GACnn_n'nnGTC
Cuts at: 1332 2256 8952 9687 1332
Size: 924 6696 735 4694
Fragments arranged by size:
6696 4694 924 735
AleI CACnn'nnGTG
Cuts at: 1135 1360 1795 2428 2737 3379 5282 6161 7192
Size: 225 435 633 309 642 1903 879 1031
Cuts at: 7192 7738 1135
Size: 546 6446
Fragments arranged by size:
6446 1903 1031 879 642 633 546 435
309 225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1153 1490 3769 5054 7620 9484 1153
Size: 337 2279 1285 2566 1864 4718
Fragments arranged by size:
4718 2566 2279 1864 1285 337
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 434 466 434
Size: 32 13017
AlwNI CAG_nnn'CTG
Cuts at: 499 874 1104 3064 3762 5775 5841 6470 7315
Size: 375 230 1960 698 2013 66 629 845
Cuts at: 7315 7980 8400 8485 8506 8695 9949 10354 11921
Size: 665 420 85 21 189 1254 405 1567
Cuts at: 11921 499
Size: 1627
Fragments arranged by size:
2013 1960 1627 1567 1254 845 698 665
629 420 405 375 230 189 85 66
21
ApaI G_GGCC'C
Cuts at: 410 4142 4639 4972 5116 5969 6576 6960 7128
Size: 3732 497 333 144 853 607 384 168
Cuts at: 7128 9117 9916 9962 410
Size: 1989 799 46 3497
Fragments arranged by size:
3732 3497 1989 853 799 607 497 384
333 168 144 46
ApaLI G'TGCA_C
Cuts at: 1846 2638 3571 4961 6374 7615 7911 11819 1846
Size: 792 933 1390 1413 1241 296 3908 3076
Fragments arranged by size:
3908 3076 1413 1390 1241 933 792 296
ApoI r'AATT_y
Cuts at: 10053 12402 12658 10053
Size: 2349 256 10444
Fragments arranged by size:
10444 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 13049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 13049
AvaI C'yCGr_G
Cuts at: 139 594 600 822 835 913 941 979 993
Size: 455 6 222 13 78 28 38 14
Cuts at: 993 1672 2291 2906 3994 5146 5595 6566 7118
Size: 679 619 615 1088 1152 449 971 552
Cuts at: 7118 8441 9176 9488 9554 9593 9707 10069 139
Size: 1323 735 312 66 39 114 362 3119
Fragments arranged by size:
3119 1323 1152 1088 971 735 679 619
615 552 455 449 362 312 222 114
78 66 39 38 28 14 13 6
AvrII C'CTAG_G
Cuts at: 4953 4953
Size: 13049
BamHI G'GATC_C
Cuts at: 7179 7826 10074 7179
Size: 647 2248 10154
Fragments arranged by size:
10154 2248 647
BanI G'GyrC_C
Cuts at: 221 512 641 706 921 1107 1129 1181 1923
Size: 291 129 65 215 186 22 52 742
Cuts at: 1923 2187 2422 4075 4570 4624 4775 5248 5468
Size: 264 235 1653 495 54 151 473 220
Cuts at: 5468 6098 6155 6995 7396 8026 8578 9023 9165
Size: 630 57 840 401 630 552 445 142
Cuts at: 9165 9181 9200 9502 9549 9726 9737 10065 10479
Size: 16 19 302 47 177 11 328 414
Cuts at: 10479 10727 10762 221
Size: 248 35 2508
Fragments arranged by size:
2508 1653 840 742 630 630 552 495
473 445 414 401 328 302 291 264
248 235 220 215 186 177 151 142
129 65 57 54 52 47 35 22
19 16 11
BanII G_rGCy'C
Cuts at: 371 410 579 601 3149 3358 4142 4256 4415
Size: 39 169 22 2548 209 784 114 159
Cuts at: 4415 4639 4972 5116 5596 5969 6114 6211 6358
Size: 224 333 144 480 373 145 97 147
Cuts at: 6358 6414 6576 6626 6665 6960 6970 7128 7868
Size: 56 162 50 39 295 10 158 740
Cuts at: 7868 8150 8333 9016 9117 9449 9700 9916 9962
Size: 282 183 683 101 332 251 216 46
Cuts at: 9962 10063 11093 371
Size: 101 1030 2327
Fragments arranged by size:
2548 2327 1030 784 740 683 480 373
333 332 295 282 251 224 216 209
183 169 162 159 158 147 145 144
114 101 101 97 56 50 46 39
39 22 10
BbeI G_GCGC'C
Cuts at: 516 925 1185 4628 4779 8030 8582 9027 9506
Size: 409 260 3443 151 3251 552 445 479
Cuts at: 9506 10483 10731 516
Size: 977 248 2834
Fragments arranged by size:
3443 3251 2834 977 552 479 445 409
260 248 151
BbsI GAAGACnn'nnnn_
Cuts at: 299 802 3297 4029 8266 299
Size: 503 2495 732 4237 5082
Fragments arranged by size:
5082 4237 2495 732 503
BbvCI CC'TCA_GC
Cuts at: 193 2322 3314 4028 7948 193
Size: 2129 992 714 3920 5294
Fragments arranged by size:
5294 3920 2129 992 714
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4636 4670 7530 7564 7902 7936 9469 9503 4636
Size: 34 2860 34 338 34 1533 34 8182
Fragments arranged by size:
8182 2860 1533 338 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3274 3657 5172 6702 10947 11708 13049 3274
Size: 383 1515 1530 4245 761 1341 3274
Fragments arranged by size:
4245 3274 1530 1515 1341 761 383
BclI T'GATC_A
Cuts at: 2625 10569 2625
Size: 7944 5105
BfrBI ATG'CAT
Cuts at: 9419 9419
Size: 13049
BglI GCCn_nnn'nGGC
Cuts at: 112 129 1174 1904 4619 5357 5696 6194 7840
Size: 17 1045 730 2715 738 339 498 1646
Cuts at: 7840 8396 8645 9477 9672 9735 112
Size: 556 249 832 195 63 3426
Fragments arranged by size:
3426 2715 1646 1045 832 738 730 556
498 339 249 195 63 17
BglII A'GATC_T
Cuts at: 3053 6508 10564 12601 3053
Size: 3455 4056 2037 3501
Fragments arranged by size:
4056 3501 3455 2037
BlpI GC'TnA_GC
Cuts at: 243 4996 6734 7084 7792 8688 10161 243
Size: 4753 1738 350 708 896 1473 3131
Fragments arranged by size:
4753 3131 1738 1473 896 708 350
Bme1580I G_kGCm'C
Cuts at: 224 410 709 1047 1850 2425 2619 2642 2691
Size: 186 299 338 803 575 194 23 49
Cuts at: 2691 3242 3575 4078 4142 4573 4615 4639 4965
Size: 551 333 503 64 431 42 24 326
Cuts at: 4965 4972 5116 5969 6158 6190 6378 6397 6560
Size: 7 144 853 189 32 188 19 163
Cuts at: 6560 6576 6960 7128 7619 7915 8610 9117 9186
Size: 16 384 168 491 296 695 507 69
Cuts at: 9186 9274 9552 9916 9962 10674 10767 11823 224
Size: 88 278 364 46 712 93 1056 1450
Fragments arranged by size:
1450 1056 853 803 712 695 575 551
507 503 491 431 384 364 338 333
326 299 296 278 194 189 188 186
168 163 144 93 88 69 64 49
46 42 32 24 23 19 16 7
BmgBI CAC'GTC
Cuts at: 2766 2967 3576 8748 9983 2766
Size: 201 609 5172 1235 5832
Fragments arranged by size:
5832 5172 1235 609 201
BmrI ACTGGGnnnn_n'
Cuts at: 2808 4667 7103 7203 7368 10261 10342 10677 12821
Size: 1859 2436 100 165 2893 81 335 2144
Cuts at: 12821 2808
Size: 3036
Fragments arranged by size:
3036 2893 2436 2144 1859 335 165 100
81
BmtI G_CTAG'C
Cuts at: 4108 4108
Size: 13049
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 543 887 3168 10056 11416 543
Size: 344 2281 6888 1360 2176
Fragments arranged by size:
6888 2281 2176 1360 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 2818 3239 3491 5686 6640 7021 7504 7723
Size: 1572 421 252 2195 954 381 483 219
Cuts at: 7723 7735 8178 8377 8701 9463 1246
Size: 12 443 199 324 762 4832
Fragments arranged by size:
4832 2195 1572 954 762 483 443 421
381 324 252 219 199 12
Bpu10I CC'TnA_GC
Cuts at: 193 288 413 1154 2322 3314 3443 4028 7948
Size: 95 125 741 1168 992 129 585 3920
Cuts at: 7948 9188 9394 193
Size: 1240 206 3848
Fragments arranged by size:
3920 3848 1240 1168 992 741 585 206
129 125 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3964 4911 7260 7792 9633 10225 11104 11596 11894
Size: 947 2349 532 1841 592 879 492 298
Cuts at: 11894 3964
Size: 5119
Fragments arranged by size:
5119 2349 1841 947 879 592 532 492
298
BsaI GGTCTCn'nnnn_
Cuts at: 528 952 1420 2235 2288 3021 4399 5721 12753
Size: 424 468 815 53 733 1378 1322 7032
Cuts at: 12753 528
Size: 824
Fragments arranged by size:
7032 1378 1322 824 815 733 468 424
53
BsaAI yAC'GTr
Cuts at: 1758 4114 4179 4481 5796 7740 10402 1758
Size: 2356 65 302 1315 1944 2662 4405
Fragments arranged by size:
4405 2662 2356 1944 1315 302 65
BsaBI GATnn'nnATC
Cuts at: 6 10563 12600 6
Size: 10557 2037 455
Fragments arranged by size:
10557 2037 455
BsaHI Gr'CG_yC
Cuts at: 449 513 922 1166 1182 1832 4034 4553 4625
Size: 64 409 244 16 650 2202 519 72
Cuts at: 4625 4776 6542 7069 8027 8060 8579 9024 9503
Size: 151 1766 527 958 33 519 445 479
Cuts at: 9503 10480 10728 11430 12926 449
Size: 977 248 702 1496 572
Fragments arranged by size:
2202 1766 1496 977 958 702 650 572
527 519 519 479 445 409 248 244
151 72 64 33 16
BsaWI w'CCGG_w
Cuts at: 300 775 3248 4504 9146 10238 10759 11437 11711
Size: 475 2473 1256 4642 1092 521 678 274
Cuts at: 11711 11858 12269 300
Size: 147 411 1080
Fragments arranged by size:
4642 2473 1256 1092 1080 678 521 475
411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 152 182 735 765 3531 3561 4159 4189 6628
Size: 30 553 30 2766 30 598 30 2439
Cuts at: 6628 6658 9561 9591 152
Size: 30 2903 30 3610
Fragments arranged by size:
3610 2903 2766 2439 598 553 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 217 442 516 1462 1724 3331 3925 4171
Size: 51 225 74 946 262 1607 594 246
Cuts at: 4171 5822 5839 7018 7327 7858 8203 8503 8563
Size: 1651 17 1179 309 531 345 300 60
Cuts at: 8563 8583 8824 8827 8832 8905 9322 9861 9873
Size: 20 241 3 5 73 417 539 12
Cuts at: 9873 166
Size: 3342
Fragments arranged by size:
3342 1651 1607 1179 946 594 539 531
417 345 309 300 262 246 241 225
74 73 60 51 20 17 12 5
3
BseYI C'CCAG_C
Cuts at: 207 230 432 668 679 2225 3309 3693 3981
Size: 23 202 236 11 1546 1084 384 288
Cuts at: 3981 5327 5527 5908 6945 7878 8054 8076 8511
Size: 1346 200 381 1037 933 176 22 435
Cuts at: 8511 9116 9635 9808 10474 11232 11809 12305 207
Size: 605 519 173 666 758 577 496 951
Fragments arranged by size:
1546 1346 1084 1037 951 933 758 666
605 577 519 496 435 384 381 288
236 202 200 176 173 23 22 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3298 3553 3847 4560 4765 5182 5542 5840 6890
Size: 255 294 713 205 417 360 298 1050
Cuts at: 6890 7570 7918 7966 7993 8252 9227 9413 3298
Size: 680 348 48 27 259 975 186 6934
Fragments arranged by size:
6934 1050 975 713 680 417 360 348
298 294 259 255 205 186 48 27
BsiEI CG_ry'CG
Cuts at: 104 124 151 1383 1605 2674 4539 6310 8619
Size: 20 27 1232 222 1069 1865 1771 2309
Cuts at: 8619 9252 9676 10591 10637 11845 104
Size: 633 424 915 46 1208 1308
Fragments arranged by size:
2309 1865 1771 1308 1232 1208 1069 915
633 424 222 46 27 20
BsiHKAI G_wGCw'C
Cuts at: 1622 1850 2642 3575 3614 4016 4085 4289 4415
Size: 228 792 933 39 402 69 204 126
Cuts at: 4415 4965 6170 6378 6482 6665 7106 7619 7868
Size: 550 1205 208 104 183 441 513 249
Cuts at: 7868 7915 9000 9571 9700 10063 10841 11031 11823
Size: 47 1085 571 129 363 778 190 792
Cuts at: 11823 1622
Size: 2848
Fragments arranged by size:
2848 1205 1085 933 792 792 778 571
550 513 441 402 363 249 228 208
204 190 183 129 126 104 69 47
39
BsiWI C'GTAC_G
Cuts at: 1264 1264
Size: 13049
BsmI GAATG_Cn'
Cuts at: 2160 2781 5928 9421 2160
Size: 621 3147 3493 5788
Fragments arranged by size:
5788 3493 3147 621
BsmBI CGTCTCn'nnnn_
Cuts at: 8595 9989 8595
Size: 1394 11655
Bsp1286I G_dGCh'C
Cuts at: 224 371 410 579 601 709 1047 1622 1850
Size: 147 39 169 22 108 338 575 228
Cuts at: 1850 2425 2619 2642 2691 3149 3242 3358 3575
Size: 575 194 23 49 458 93 116 217
Cuts at: 3575 3614 4016 4078 4085 4142 4256 4289 4415
Size: 39 402 62 7 57 114 33 126
Cuts at: 4415 4573 4615 4639 4965 4972 5116 5596 5969
Size: 158 42 24 326 7 144 480 373
Cuts at: 5969 6114 6158 6170 6190 6211 6358 6378 6397
Size: 145 44 12 20 21 147 20 19
Cuts at: 6397 6414 6482 6560 6576 6626 6665 6960 6970
Size: 17 68 78 16 50 39 295 10
Cuts at: 6970 7106 7128 7619 7868 7915 8150 8333 8610
Size: 136 22 491 249 47 235 183 277
Cuts at: 8610 9000 9016 9117 9186 9274 9449 9552 9571
Size: 390 16 101 69 88 175 103 19
Cuts at: 9571 9700 9916 9962 10063 10674 10767 10841 11031
Size: 129 216 46 101 611 93 74 190
Cuts at: 11031 11093 11823 224
Size: 62 730 1450
Fragments arranged by size:
1450 730 611 575 575 491 480 458
402 390 373 338 326 295 277 249
235 228 217 216 194 190 183 175
169 158 147 147 145 144 136 129
126 116 114 108 103 101 101 93
93 88 78 74 69 68 62 62
57 50 49 47 46 44 42 39
39 39 33 24 23 22 22 21
20 20 19 19 17 16 16 12
10 7 7
BspEI T'CCGG_A
Cuts at: 300 775 3248 12269 300
Size: 475 2473 9021 1080
Fragments arranged by size:
9021 2473 1080 475
BspHI T'CATG_A
Cuts at: 1208 2622 7421 12225 1208
Size: 1414 4799 4804 2032
Fragments arranged by size:
4804 4799 2032 1414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1316 1517 1755 2816 4582 5204 6028 6110 6824
Size: 201 238 1061 1766 622 824 82 714
Cuts at: 6824 7989 9335 10099 10313 10615 10996 1316
Size: 1165 1346 764 214 302 381 3369
Fragments arranged by size:
3369 1766 1346 1165 1061 824 764 714
622 381 302 238 214 201 82
BsrBI CCG'CTC
Cuts at: 50 1220 1253 4754 8612 11341 11395 12879 13044
Size: 1170 33 3501 3858 2729 54 1484 165
Cuts at: 13044 50
Size: 55
Fragments arranged by size:
3858 3501 2729 1484 1170 165 55 54
33
BsrDI GCAATG_nn'
Cuts at: 3753 4909 8949 10961 3753
Size: 1156 4040 2012 5841
Fragments arranged by size:
5841 4040 2012 1156
BsrFI r'CCGG_y
Cuts at: 657 918 1168 1398 1985 2673 4534 5645 5690
Size: 261 250 230 587 688 1861 1111 45
Cuts at: 5690 6095 6898 9146 9398 9734 11047 11228 11437
Size: 405 803 2248 252 336 1313 181 209
Cuts at: 11437 657
Size: 2269
Fragments arranged by size:
2269 2248 1861 1313 1111 803 688 587
405 336 261 252 250 230 209 181
45
BssHII G'CGCG_C
Cuts at: 712 1540 1542 4457 4657 712
Size: 828 2 2915 200 9104
Fragments arranged by size:
9104 2915 828 200 2
BssSI C'ACGA_G
Cuts at: 4813 8143 9746 11320 11678 4813
Size: 3330 1603 1574 358 6184
Fragments arranged by size:
6184 3330 1603 1574 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2137 3885 4914 5658 6913 7980 8231 10426 2137
Size: 1748 1029 744 1255 1067 251 2195 4760
Fragments arranged by size:
4760 2195 1748 1255 1067 1029 744 251
BstBI TT'CG_AA
Cuts at: 11410 11410
Size: 13049
BstEII G'GTnAC_C
Cuts at: 2880 2880
Size: 13049
BstXI CCAn_nnnn'nTGG
Cuts at: 1359 1362 1842 3781 4863 5410 5754 6197 6729
Size: 3 480 1939 1082 547 344 443 532
Cuts at: 6729 7062 8742 8934 1359
Size: 333 1680 192 5474
Fragments arranged by size:
5474 1939 1680 1082 547 532 480 443
344 333 192 3
BstYI r'GATC_y
Cuts at: 1 826 1910 3053 3626 6508 7179 7319 7826
Size: 825 1084 1143 573 2882 671 140 507
Cuts at: 7826 8132 10074 10541 10564 10899 11145 12157 12243
Size: 306 1942 467 23 335 246 1012 86
Cuts at: 12243 12255 12601 1
Size: 12 346 449
Fragments arranged by size:
2882 1942 1143 1084 1012 825 671 573
507 467 449 346 335 306 246 140
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 585 5834 7353 7471 8102 8486 8659 585
Size: 5249 1519 118 631 384 173 4975
Fragments arranged by size:
5249 4975 1519 631 384 173 118
BtgI C'CryG_G
Cuts at: 127 645 1133 1329 1875 2145 2298 4844 4862
Size: 518 488 196 546 270 153 2546 18
Cuts at: 4862 5021 5252 5409 5464 5747 6117 6190 6310
Size: 159 231 157 55 283 370 73 120
Cuts at: 6310 6713 7055 7216 7455 8022 9472 9506 9813
Size: 403 342 161 239 567 1450 34 307
Cuts at: 9813 127
Size: 3363
Fragments arranged by size:
3363 2546 1450 567 546 518 488 403
370 342 307 283 270 239 231 196
161 159 157 153 120 73 55 34
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4338 10999 11180 11343 4338
Size: 6661 181 163 6044
Fragments arranged by size:
6661 6044 181 163
BtsI GCAGTG_nn'
Cuts at: 2628 3230 3374 3386 3886 5823 6190 6285 6286
Size: 602 144 12 500 1937 367 95 1
Cuts at: 6286 6324 6397 6794 6907 7558 7913 8610 9923
Size: 38 73 397 113 651 355 697 1313
Cuts at: 9923 10292 2628
Size: 369 5385
Fragments arranged by size:
5385 1937 1313 697 651 602 500 397
369 367 355 144 113 95 73 38
12 1
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1857 1892 5289 5324 7803 7838 1857
Size: 35 3397 35 2479 35 7068
Fragments arranged by size:
7068 3397 2479 35 35 35
DraI TTT'AAA
Cuts at: 10048 10048
Size: 13049
DraIII CAC_nnn'GTG
Cuts at: 3870 5919 3870
Size: 2049 11000
DrdI GACnn_nn'nnGTC
Cuts at: 3709 10755 11613 3709
Size: 7046 858 5145
Fragments arranged by size:
7046 5145 858
EaeI y'GGCC_r
Cuts at: 121 1349 1364 1602 1685 1811 1983 1987 2015
Size: 1228 15 238 83 126 172 4 28
Cuts at: 2015 2671 2719 3087 3920 4536 4821 5018 5208
Size: 656 48 368 833 616 285 197 190
Cuts at: 5208 5643 5688 6417 6548 6756 7219 8616 8750
Size: 435 45 729 131 208 463 1397 134
Cuts at: 8750 8856 8881 8962 9400 9513 9673 10634 10808
Size: 106 25 81 438 113 160 961 174
Cuts at: 10808 11199 11226 12571 121
Size: 391 27 1345 599
Fragments arranged by size:
1397 1345 1228 961 833 729 656 616
599 463 438 435 391 368 285 238
208 197 190 174 172 160 134 131
126 113 106 83 81 48 45 28
27 25 15 4
EagI C'GGCC_G
Cuts at: 121 1602 2671 4536 8616 9673 10634 121
Size: 1481 1069 1865 4080 1057 961 2536
Fragments arranged by size:
4080 2536 1865 1481 1069 1057 961
EarI CTCTTCn'nnn_
Cuts at: 955 3637 3730 4768 5123 5740 6637 7876 7972
Size: 2682 93 1038 355 617 897 1239 96
Cuts at: 7972 8422 9312 11072 11282 955
Size: 450 890 1760 210 2722
Fragments arranged by size:
2722 2682 1760 1239 1038 897 890 617
450 355 210 96 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 296 552 816 3568 4463 7588 9569 11257 11567
Size: 256 264 2752 895 3125 1981 1688 310
Cuts at: 11567 11713 12859 296
Size: 146 1146 486
Fragments arranged by size:
3125 2752 1981 1688 1146 895 486 310
264 256 146
EcoICRI GAG'CTC
Cuts at: 4413 6663 7866 9698 10061 4413
Size: 2250 1203 1832 363 7401
Fragments arranged by size:
7401 2250 1832 1203 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 1034 1246 2818 3239 3491 5322 5686 5797
Size: 426 212 1572 421 252 1831 364 111
Cuts at: 5797 6640 7021 7253 7504 7723 7735 8178 8377
Size: 843 381 232 251 219 12 443 199
Cuts at: 8377 8701 8822 9160 9463 10874 11306 12053 12321
Size: 324 121 338 303 1411 432 747 268
Cuts at: 12321 608
Size: 1336
Fragments arranged by size:
1831 1572 1411 1336 843 747 443 432
426 421 381 364 338 324 303 268
252 251 232 219 212 199 121 111
12
EcoNI CCTnn'n_nnAGG
Cuts at: 196 878 3392 5737 196
Size: 682 2514 2345 7508
Fragments arranged by size:
7508 2514 2345 682
EcoO109I rG'GnC_Cy
Cuts at: 135 234 331 406 407 544 591 818 1158
Size: 99 97 75 1 137 47 227 340
Cuts at: 1158 2856 3397 4138 4139 4635 4968 4969 5113
Size: 1698 541 741 1 496 333 1 144
Cuts at: 5113 5193 5293 5508 5772 5966 5994 6572 6573
Size: 80 100 215 264 194 28 578 1
Cuts at: 6573 6957 7124 7557 7628 7661 7671 7892 8397
Size: 384 167 433 71 33 10 221 505
Cuts at: 8397 8515 8522 9407 9597 9654 9913 9959 10188
Size: 118 7 885 190 57 259 46 229
Cuts at: 10188 135
Size: 2996
Fragments arranged by size:
2996 1698 885 741 578 541 505 496
433 384 340 333 264 259 229 227
221 215 194 190 167 144 137 118
100 99 97 80 75 71 57 47
46 33 28 10 7 1 1 1
1
EcoRI G'AATT_C
Cuts at: 10053 10053
Size: 13049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4812 5295 11068 4812
Size: 483 5773 6793
Fragments arranged by size:
6793 5773 483
FseI GG_CCGG'CC
Cuts at: 1989 4538 1989
Size: 2549 10500
FspI TGC'GCA
Cuts at: 1963 7801 10830 1963
Size: 5838 3029 4182
Fragments arranged by size:
5838 4182 3029
HaeII r_GCGC'y
Cuts at: 392 516 925 978 1185 1755 2219 3966 4105
Size: 124 409 53 207 570 464 1747 139
Cuts at: 4105 4133 4195 4628 4779 5328 6946 8030 8485
Size: 28 62 433 151 549 1618 1084 455
Cuts at: 8485 8582 8874 9027 9123 9506 10483 10731 11753
Size: 97 292 153 96 383 977 248 1022
Cuts at: 11753 12732 392
Size: 979 709
Fragments arranged by size:
1747 1618 1084 1022 979 977 709 570
549 464 455 433 409 383 292 248
207 153 151 139 124 97 96 62
53 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 735 767 1318 1350 1564 1596 2242 2274 2287
Size: 32 551 32 214 32 646 32 13
Cuts at: 2287 2319 2538 2570 2818 2850 3181 3213 3451
Size: 32 219 32 248 32 331 32 238
Cuts at: 3451 3483 5100 5132 7512 7544 7884 7916 8770
Size: 32 1617 32 2380 32 340 32 854
Cuts at: 8770 8802 8938 8970 9673 9705 10891 10921 10923
Size: 32 136 32 703 32 1186 30 2
Cuts at: 10923 10953 735
Size: 30 2831
Fragments arranged by size:
2831 2380 1617 1186 854 703 646 551
340 331 248 238 219 214 136 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 30 30
13 2
HincII GTy'rAC
Cuts at: 152 3212 3588 3771 8343 10088 152
Size: 3060 376 183 4572 1745 3113
Fragments arranged by size:
4572 3113 3060 1745 376 183
KasI G'GCGC_C
Cuts at: 512 921 1181 4624 4775 8026 8578 9023 9502
Size: 409 260 3443 151 3251 552 445 479
Cuts at: 9502 10479 10727 512
Size: 977 248 2834
Fragments arranged by size:
3443 3251 2834 977 552 479 445 409
260 248 151
KpnI G_GTAC'C
Cuts at: 5472 10069 5472
Size: 4597 8452
MluI A'CGCG_T
Cuts at: 1143 12482 1143
Size: 11339 1710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2024 3674 3740 4362 4454 4880 8807 8901 9815
Size: 1650 66 622 92 426 3927 94 914
Cuts at: 9815 11720 11904 2024
Size: 1905 184 3169
Fragments arranged by size:
3927 3169 1905 1650 914 622 426 184
94 92 66
MscI TGG'CCA
Cuts at: 2017 3922 4823 6550 6758 8752 8964 10810 2017
Size: 1905 901 1727 208 1994 212 1846 4256
Fragments arranged by size:
4256 1994 1905 1846 1727 901 212 208
MslI CAynn'nnrTG
Cuts at: 275 1135 1357 1360 1708 1795 2026 2428 2633
Size: 860 222 3 348 87 231 402 205
Cuts at: 2633 2737 3083 3379 3841 5204 5282 5408 5452
Size: 104 346 296 462 1363 78 126 44
Cuts at: 5452 5752 6161 6878 7190 7192 7622 7738 7835
Size: 300 409 717 312 2 430 116 97
Cuts at: 7835 8191 8765 11038 11165 275
Size: 356 574 2273 127 2159
Fragments arranged by size:
2273 2159 1363 860 717 574 462 430
409 402 356 348 346 312 300 296
231 222 205 127 126 116 104 97
87 78 44 3 2
MspA1I CmG'CkG
Cuts at: 672 956 1135 1470 1537 1552 1601 1995 2416
Size: 284 179 335 67 15 49 394 421
Cuts at: 2416 3313 3759 3978 4027 4373 4541 5342 6312
Size: 897 446 219 49 346 168 801 970
Cuts at: 6312 6386 6434 6467 6738 6954 7905 8024 8643
Size: 74 48 33 271 216 951 119 619
Cuts at: 8643 8695 9238 9321 9639 9678 9717 9774 10160
Size: 52 543 83 318 39 39 57 386
Cuts at: 10160 10474 10834 11847 12092 672
Size: 314 360 1013 245 1629
Fragments arranged by size:
1629 1013 970 951 897 801 619 543
446 421 394 386 360 346 335 318
314 284 271 245 219 216 179 168
119 83 74 67 57 52 49 49
48 39 39 33 15
NaeI GCC'GGC
Cuts at: 920 1170 1987 4536 5692 6097 6900 9400 9736
Size: 250 817 2549 1156 405 803 2500 336
Cuts at: 9736 11230 920
Size: 1494 2739
Fragments arranged by size:
2739 2549 2500 1494 1156 817 803 405
336 250
NarI GG'CG_CC
Cuts at: 513 922 1182 4625 4776 8027 8579 9024 9503
Size: 409 260 3443 151 3251 552 445 479
Cuts at: 9503 10480 10728 513
Size: 977 248 2834
Fragments arranged by size:
3443 3251 2834 977 552 479 445 409
260 248 151
NcoI C'CATG_G
Cuts at: 2145 2298 5252 5464 5747 6117 6713 7055 9506
Size: 153 2954 212 283 370 596 342 2451
Cuts at: 9506 9813 2145
Size: 307 5381
Fragments arranged by size:
5381 2954 2451 596 370 342 307 283
212 153
NgoMIV G'CCGG_C
Cuts at: 918 1168 1985 4534 5690 6095 6898 9398 9734
Size: 250 817 2549 1156 405 803 2500 336
Cuts at: 9734 11228 918
Size: 1494 2739
Fragments arranged by size:
2739 2549 2500 1494 1156 817 803 405
336 250
NheI G'CTAG_C
Cuts at: 4104 4104
Size: 13049
NotI GC'GGCC_GC
Cuts at: 121 121
Size: 13049
NruI TCG'CGA
Cuts at: 1436 4738 1436
Size: 3302 9747
NsiI A_TGCA'T
Cuts at: 9421 9421
Size: 13049
NspI r_CATG'y
Cuts at: 936 3954 7998 10102 10602 11509 936
Size: 3018 4044 2104 500 907 2476
Fragments arranged by size:
4044 3018 2476 2104 907 500
PasI CC'CwG_GG
Cuts at: 2526 6576 6609 6961 7851 8049 8970 2526
Size: 4050 33 352 890 198 921 6605
Fragments arranged by size:
6605 4050 921 890 352 198 33
PciI A'CATG_T
Cuts at: 3950 11505 3950
Size: 7555 5494
PflMI CCAn_nnn'nTGG
Cuts at: 459 1104 1920 2031 2225 3273 3426 6217 6819
Size: 645 816 111 194 1048 153 2791 602
Cuts at: 6819 7315 8049 8400 8506 459
Size: 496 734 351 106 5002
Fragments arranged by size:
5002 2791 1048 816 734 645 602 496
351 194 153 111 106
PfoI T'CCnGG_A
Cuts at: 317 2795 5738 7481 317
Size: 2478 2943 1743 5885
Fragments arranged by size:
5885 2943 2478 1743
PmeI GTTT'AAAC
Cuts at: 10048 10048
Size: 13049
PmlI CAC'GTG
Cuts at: 4114 4481 5796 7740 4114
Size: 367 1315 1944 9423
Fragments arranged by size:
9423 1944 1315 367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1204 1236 12188 12220 1204
Size: 32 10952 32 2033
Fragments arranged by size:
10952 2033 32 32
PpuMI rG'GwC_Cy
Cuts at: 234 544 591 1158 5193 5508 5994 7557 7628
Size: 310 47 567 4035 315 486 1563 71
Cuts at: 7628 7661 7671 7892 8515 9407 9597 9654 234
Size: 33 10 221 623 892 190 57 3629
Fragments arranged by size:
4035 3629 1563 892 623 567 486 315
310 221 190 71 57 47 33 10
PshAI GACnn'nnGTC
Cuts at: 495 3256 495
Size: 2761 10288
PspOMI G'GGCC_C
Cuts at: 406 4138 4635 4968 5112 5965 6572 6956 7124
Size: 3732 497 333 144 853 607 384 168
Cuts at: 7124 9113 9912 9958 406
Size: 1989 799 46 3497
Fragments arranged by size:
3732 3497 1989 853 799 607 497 384
333 168 144 46
PspXI vC'TCGA_Gb
Cuts at: 3994 3994
Size: 13049
PstI C_TGCA'G
Cuts at: 1469 1551 3053 3381 3758 4137 4166 5033 5771
Size: 82 1502 328 377 379 29 867 738
Cuts at: 5771 7651 7782 7920 9005 10096 10781 1469
Size: 1880 131 138 1085 1091 685 3737
Fragments arranged by size:
3737 1880 1502 1091 1085 867 738 685
379 377 328 138 131 82 29
PvuI CG_AT'CG
Cuts at: 104 104
Size: 13049
PvuII CAG'CTG
Cuts at: 1470 1537 1552 2416 3313 3759 5342 6434 7905
Size: 67 15 864 897 446 1583 1092 1471
Cuts at: 7905 9238 9321 9717 9774 10474 10834 1470
Size: 1333 83 396 57 700 360 3685
Fragments arranged by size:
3685 1583 1471 1333 1092 897 864 700
446 396 360 83 67 57 15
RsrII CG'GwC_CG
Cuts at: 11244 11244
Size: 13049
SacI G_AGCT'C
Cuts at: 4415 6665 7868 9700 10063 4415
Size: 2250 1203 1832 363 7401
Fragments arranged by size:
7401 2250 1832 1203 363
SacII CC_GC'GG
Cuts at: 1136 6313 8025 1136
Size: 5177 1712 6160
Fragments arranged by size:
6160 5177 1712
SalI G'TCGA_C
Cuts at: 150 10086 150
Size: 9936 3113
SanDI GG'GwC_CC
Cuts at: 234 591 7557 7628 9407 9654 234
Size: 357 6966 71 1779 247 3629
Fragments arranged by size:
6966 3629 1779 357 247 71
SapI GCTCTTCn'nnn_
Cuts at: 4768 7972 8422 11072 11282 4768
Size: 3204 450 2650 210 6535
Fragments arranged by size:
6535 3204 2650 450 210
SbfI CC_TGCA'GG
Cuts at: 10096 10096
Size: 13049
ScaI AGT'ACT
Cuts at: 2170 12566 2170
Size: 10396 2653
SexAI A'CCwGG_T
Cuts at: 2876 5624 5682 9722 2876
Size: 2748 58 4040 6203
Fragments arranged by size:
6203 4040 2748 58
SfcI C'TryA_G
Cuts at: 33 1465 1547 3049 3377 3754 4133 4162 5029
Size: 1432 82 1502 328 377 379 29 867
Cuts at: 5029 5767 7516 7647 7778 7916 9001 10092 10777
Size: 738 1749 131 131 138 1085 1091 685
Cuts at: 10777 11770 11961 33
Size: 993 191 1121
Fragments arranged by size:
1749 1502 1432 1121 1091 1085 993 867
738 685 379 377 328 191 138 131
131 82 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 5696 8396 5696
Size: 2700 10349
SfoI GGC'GCC
Cuts at: 514 923 1183 4626 4777 8028 8580 9025 9504
Size: 409 260 3443 151 3251 552 445 479
Cuts at: 9504 10481 10729 514
Size: 977 248 2834
Fragments arranged by size:
3443 3251 2834 977 552 479 445 409
260 248 151
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 13049
SgrAI Cr'CCGG_yG
Cuts at: 1168 1168
Size: 13049
SmaI CCC'GGG
Cuts at: 596 943 981 995 1674 6568 7120 9595 10071
Size: 347 38 14 679 4894 552 2475 476
Cuts at: 10071 596
Size: 3574
Fragments arranged by size:
4894 3574 2475 679 552 476 347 38
14
SmlI C'TyrA_G
Cuts at: 2291 3943 3994 4890 7275 7807 9554 9648 10204
Size: 1652 51 896 2385 532 1747 94 556
Cuts at: 10204 11119 11611 11873 2291
Size: 915 492 262 3467
Fragments arranged by size:
3467 2385 1747 1652 915 896 556 532
492 262 94 51
SpeI A'CTAG_T
Cuts at: 67 67
Size: 13049
SphI G_CATG'C
Cuts at: 936 7998 10102 10602 936
Size: 7062 2104 500 3383
Fragments arranged by size:
7062 3383 2104 500
StuI AGG'CCT
Cuts at: 5804 5804
Size: 13049
StyI C'CwwG_G
Cuts at: 262 2102 2145 2298 4953 4976 5252 5464 5747
Size: 1840 43 153 2655 23 276 212 283
Cuts at: 5747 6117 6414 6713 7055 8804 9215 9506 9756
Size: 370 297 299 342 1749 411 291 250
Cuts at: 9756 9813 9908 10183 10537 262
Size: 57 95 275 354 2774
Fragments arranged by size:
2774 2655 1840 1749 411 370 354 342
299 297 291 283 276 275 250 212
153 95 57 43 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2539 2758 4423 6130 6568 7204 7750 8935 9786
Size: 219 1665 1707 438 636 546 1185 851
Cuts at: 9786 10632 10765 11224 11433 2539
Size: 846 133 459 209 4155
Fragments arranged by size:
4155 1707 1665 1185 851 846 636 546
459 438 219 209 133
TatI w'GTAC_w
Cuts at: 1088 2168 3098 12564 1088
Size: 1080 930 9466 1573
Fragments arranged by size:
9466 1573 1080 930
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3290 3322 3531 3563 6169 6201 9561 9593 12566
Size: 32 209 32 2606 32 3360 32 2973
Cuts at: 12566 12598 3290
Size: 32 3741
Fragments arranged by size:
3741 3360 2973 2606 209 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 8668 9796 10846 8668
Size: 1128 1050 10871
Fragments arranged by size:
10871 1128 1050
XbaI T'CTAG_A
Cuts at: 9310 10080 9310
Size: 770 12279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 676 1359 1605 2025 4354 4983 5097 5407 6197
Size: 683 246 420 2329 629 114 310 790
Cuts at: 6197 6535 7278 8725 8767 9831 10544 676
Size: 338 743 1447 42 1064 713 3181
Fragments arranged by size:
3181 2329 1447 1064 790 743 713 683
629 420 338 310 246 114 42
XhoI C'TCGA_G
Cuts at: 2291 3994 9554 2291
Size: 1703 5560 5786
Fragments arranged by size:
5786 5560 1703
XmaI C'CCGG_G
Cuts at: 594 941 979 993 1672 6566 7118 9593 10069
Size: 347 38 14 679 4894 552 2475 476
Cuts at: 10069 594
Size: 3574
Fragments arranged by size:
4894 3574 2475 679 552 476 347 38
14
ZraI GAC'GTC
Cuts at: 4035 4035
Size: 13049
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AscI BaeI BsrGI BstZ17I ClaI EcoRV FspAI
HindIII HpaI MfeI NdeI PacI PsiI PsrI SnaBI
SrfI SspI SwaI XmnI