Restriction map for
pF1KE2301
(Circular) (Six-base) MAPSORT of: pF1KE2301.seq Check: 1129 from: 1 to: 9644
pF1KE2301 9644 bp
With 182 enzymes: SgfI *
August 9, 2016 17:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3734 5647 6086 3734
Size: 1913 439 7292
Fragments arranged by size:
7292 1913 439
AccI GT'mk_AC
Cuts at: 1948 4817 5136 6682 1948
Size: 2869 319 1546 4910
Fragments arranged by size:
4910 2869 1546 319
Acc65I G'GTAC_C
Cuts at: 5989 6660 5989
Size: 671 8973
AclI AA'CG_TT
Cuts at: 3990 9484 3990
Size: 5494 4150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 309 1509 2075 2588 2606 3069 6517 7469
Size: 140 1200 566 513 18 463 3448 952
Cuts at: 7469 7901 8648 8916 169
Size: 432 747 268 897
Fragments arranged by size:
3448 1200 952 897 747 566 513 463
432 268 140 18
AfeI AGC'GCT
Cuts at: 3013 4372 3013
Size: 1359 8285
AflII C'TTAA_G
Cuts at: 3394 3997 3394
Size: 603 9041
AflIII A'CryG_T
Cuts at: 642 1943 2298 4431 4567 4898 5056 6996 8100
Size: 1301 355 2133 136 331 158 1940 1104
Cuts at: 8100 9077 642
Size: 977 1209
Fragments arranged by size:
2133 1940 1301 1209 1104 977 355 331
158 136
AgeI A'CCGG_T
Cuts at: 2987 8032 2987
Size: 5045 4599
AhdI GACnn_n'nnGTC
Cuts at: 3926 4824 3926
Size: 898 8746
AleI CACnn'nnGTG
Cuts at: 3290 4849 3290
Size: 1559 8085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 296 1376 2924 4042 5831 296
Size: 1080 1548 1118 1789 4109
Fragments arranged by size:
4109 1789 1548 1118 1080
AlwNI CAG_nnn'CTG
Cuts at: 562 733 1827 2476 2796 3965 4476 5022 5709
Size: 171 1094 649 320 1169 511 546 687
Cuts at: 5709 5983 6168 6369 6949 8516 562
Size: 274 185 201 580 1567 1690
Fragments arranged by size:
1690 1567 1169 1094 687 649 580 546
511 320 274 201 185 171
ApaI G_GGCC'C
Cuts at: 770 3615 5524 770
Size: 2845 1909 4890
Fragments arranged by size:
4890 2845 1909
ApaLI G'TGCA_C
Cuts at: 4627 6103 8414 4627
Size: 1476 2311 5857
Fragments arranged by size:
5857 2311 1476
ApoI r'AATT_y
Cuts at: 964 1882 3070 6648 8997 9253 964
Size: 918 1188 3578 2349 256 1355
Fragments arranged by size:
3578 2349 1355 1188 918 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9644
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9644
AvaI C'yCGr_G
Cuts at: 1967 5042 5252 5523 6664 1967
Size: 3075 210 271 1141 4947
Fragments arranged by size:
4947 3075 1141 271 210
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5981 6014 5981
Size: 33 9611
BamHI G'GATC_C
Cuts at: 6669 6669
Size: 9644
BanI G'GyrC_C
Cuts at: 472 3159 3299 5820 5989 5997 6660 7074 7322
Size: 2687 140 2521 169 8 663 414 248
Cuts at: 7322 7357 472
Size: 35 2759
Fragments arranged by size:
2759 2687 2521 663 414 248 169 140
35 8
BanII G_rGCy'C
Cuts at: 770 1992 2093 3083 3615 4841 5074 5405 5524
Size: 1222 101 990 532 1226 233 331 119
Cuts at: 5524 6658 7688 770
Size: 1134 1030 2726
Fragments arranged by size:
2726 1226 1222 1134 1030 990 532 331
233 119 101
BbeI G_GCGC'C
Cuts at: 7078 7326 7078
Size: 248 9396
BbsI GAAGACnn'nnnn_
Cuts at: 4175 4175
Size: 9644
BbvCI CC'TCA_GC
Cuts at: 1109 1166 1192 1109
Size: 57 26 9561
Fragments arranged by size:
9561 57 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4947 4981 4947
Size: 34 9610
BciVI GTATCCnnnnn_n'
Cuts at: 1210 1824 3028 7542 8303 9644 1210
Size: 614 1204 4514 761 1341 1210
Fragments arranged by size:
4514 1341 1210 1204 761 614
BclI T'GATC_A
Cuts at: 4205 7164 4205
Size: 2959 6685
BfrBI ATG'CAT
Cuts at: 4695 4695
Size: 9644
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 9644
BglII A'GATC_T
Cuts at: 1592 3540 5159 7159 9196 1592
Size: 1948 1619 2000 2037 2040
Fragments arranged by size:
2040 2037 2000 1948 1619
BlpI GC'TnA_GC
Cuts at: 1721 2822 4357 4864 6756 1721
Size: 1101 1535 507 1892 4609
Fragments arranged by size:
4609 1892 1535 1101 507
Bme1580I G_kGCm'C
Cuts at: 770 1165 3162 3302 3615 4053 4631 5524 5825
Size: 395 1997 140 313 438 578 893 301
Cuts at: 5825 6002 6107 6213 7269 7362 8418 770
Size: 177 105 106 1056 93 1056 1996
Fragments arranged by size:
1997 1996 1056 1056 893 578 438 395
313 301 177 140 106 105 93
BmgBI CAC'GTC
Cuts at: 3288 3288
Size: 9644
BmrI ACTGGGnnnn_n'
Cuts at: 1784 4030 5432 6086 6495 6507 6608 6856 6937
Size: 2246 1402 654 409 12 101 248 81
Cuts at: 6937 7272 9416 1784
Size: 335 2144 2012
Fragments arranged by size:
2246 2144 2012 1402 654 409 335 248
101 81 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1614 4251 8011 1614
Size: 2637 3760 3247
Fragments arranged by size:
3760 3247 2637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 929 1714 2531 2686 4135 4813 5344 5381 5753
Size: 785 817 155 1449 678 531 37 372
Cuts at: 5753 5836 5997 929
Size: 83 161 4576
Fragments arranged by size:
4576 1449 817 785 678 531 372 161
155 83 37
Bpu10I CC'TnA_GC
Cuts at: 1109 1166 1192 2645 6254 1109
Size: 57 26 1453 3609 4499
Fragments arranged by size:
4499 3609 1453 57 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 904 3763 6820 7699 8191 8489 147
Size: 757 2859 3057 879 492 298 1302
Fragments arranged by size:
3057 2859 1302 879 757 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 616 2507 2655 9348 616
Size: 1891 148 6693 912
Fragments arranged by size:
6693 1891 912 148
BsaAI yAC'GTr
Cuts at: 2301 3951 5057 6997 2301
Size: 1650 1106 1940 4948
Fragments arranged by size:
4948 1940 1650 1106
BsaBI GATnn'nnATC
Cuts at: 6 4801 7158 9195 6
Size: 4795 2357 2037 455
Fragments arranged by size:
4795 2357 2037 455
BsaHI Gr'CG_yC
Cuts at: 2966 5759 6218 6230 7075 7323 8025 9521 2966
Size: 2793 459 12 845 248 702 1496 3089
Fragments arranged by size:
3089 2793 1496 845 702 459 248 12
BsaWI w'CCGG_w
Cuts at: 2987 5559 6833 7354 8032 8306 8453 8864 2987
Size: 2572 1274 521 678 274 147 411 3767
Fragments arranged by size:
3767 2572 1274 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1089 1119 1824 1854 3882 3912 4975 5005 5541
Size: 30 705 30 2028 30 1063 30 536
Cuts at: 5541 5571 6183 6213 1089
Size: 30 612 30 4520
Fragments arranged by size:
4520 2028 1063 705 612 536 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1097 1587 2350 2991 3402 3436 3699 3874 3877
Size: 490 763 641 411 34 263 175 3
Cuts at: 3877 3884 4777 5270 5275 5368 5769 6473 1097
Size: 7 893 493 5 93 401 704 4268
Fragments arranged by size:
4268 893 763 704 641 493 490 411
401 263 175 93 34 7 5 3
BseYI C'CCAG_C
Cuts at: 162 3155 4124 4330 4447 4760 5902 6004 6494
Size: 2993 969 206 117 313 1142 102 490
Cuts at: 6494 6506 6542 6602 7069 7827 8404 8900 162
Size: 12 36 60 467 758 577 496 906
Fragments arranged by size:
2993 1142 969 906 758 577 496 490
467 313 206 117 102 60 36 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 2323 2737 2746 3362 3395 4768 5554 410
Size: 1913 414 9 616 33 1373 786 4500
Fragments arranged by size:
4500 1913 1373 786 616 414 33 9
BsiEI CG_ry'CG
Cuts at: 104 157 3720 7186 7232 8440 104
Size: 53 3563 3466 46 1208 1308
Fragments arranged by size:
3563 3466 1308 1208 53 46
BsiHKAI G_wGCw'C
Cuts at: 1797 2045 3083 4358 4631 4841 4874 6034 6107
Size: 248 1038 1275 273 210 33 1160 73
Cuts at: 6107 6658 7436 7626 8418 1797
Size: 551 778 190 792 3023
Fragments arranged by size:
3023 1275 1160 1038 792 778 551 273
248 210 190 73 33
BsmI GAATG_Cn'
Cuts at: 854 4145 854
Size: 3291 6353
Bsp1286I G_dGCh'C
Cuts at: 770 1165 1797 1992 2045 2093 3083 3162 3302
Size: 395 632 195 53 48 990 79 140
Cuts at: 3302 3615 4053 4358 4631 4841 4874 5074 5405
Size: 313 438 305 273 210 33 200 331
Cuts at: 5405 5524 5825 6002 6034 6107 6213 6658 7269
Size: 119 301 177 32 73 106 445 611
Cuts at: 7269 7362 7436 7626 7688 8418 770
Size: 93 74 190 62 730 1996
Fragments arranged by size:
1996 990 730 632 611 445 438 395
331 313 305 301 273 210 200 195
190 177 140 119 106 93 79 74
73 62 53 48 33 32
BspEI T'CCGG_A
Cuts at: 5559 8864 5559
Size: 3305 6339
BspHI T'CATG_A
Cuts at: 3170 3480 4202 8820 3170
Size: 310 722 4618 3994
Fragments arranged by size:
4618 3994 722 310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1160 1325 1344 3631 3734 4034 5647 5661 5823
Size: 165 19 2287 103 300 1613 14 162
Cuts at: 5823 5977 6086 6694 6908 7210 7591 1160
Size: 154 109 608 214 302 381 3213
Fragments arranged by size:
3213 2287 1613 608 381 302 300 214
165 162 154 109 103 19 14
BsrBI CCG'CTC
Cuts at: 50 1398 1875 3707 5534 7936 7990 9474 9639
Size: 1348 477 1832 1827 2402 54 1484 165
Cuts at: 9639 50
Size: 55
Fragments arranged by size:
2402 1832 1827 1484 1348 477 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1621 3279 3459 4026 4400 7556 1621
Size: 1658 180 567 374 3156 3709
Fragments arranged by size:
3709 3156 1658 567 374 180
BsrFI r'CCGG_y
Cuts at: 140 722 992 1172 1276 2561 2987 3352 5240
Size: 582 270 180 104 1285 426 365 1888
Cuts at: 5240 5801 7642 7823 8032 140
Size: 561 1841 181 209 1752
Fragments arranged by size:
1888 1841 1752 1285 582 561 426 365
270 209 181 180 104
BsrGI T'GTAC_A
Cuts at: 1009 3041 5630 1009
Size: 2032 2589 5023
Fragments arranged by size:
5023 2589 2032
BssSI C'ACGA_G
Cuts at: 2676 5666 7915 8273 2676
Size: 2990 2249 358 4047
Fragments arranged by size:
4047 2990 2249 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2724 3528 3965 4692 5274 5709 6369 7021 2724
Size: 804 437 727 582 435 660 652 5347
Fragments arranged by size:
5347 804 727 660 652 582 437 435
BstBI TT'CG_AA
Cuts at: 8005 8005
Size: 9644
BstXI CCAn_nnnn'nTGG
Cuts at: 1692 3679 5210 1692
Size: 1987 1531 6126
Fragments arranged by size:
6126 1987 1531
BstYI r'GATC_y
Cuts at: 1 122 899 1592 2082 2844 3540 5159 5172
Size: 121 777 693 490 762 696 1619 13
Cuts at: 5172 6063 6365 6669 7136 7159 7494 7740 8752
Size: 891 302 304 467 23 335 246 1012
Cuts at: 8752 8838 8850 9196 1
Size: 86 12 346 449
Fragments arranged by size:
1619 1012 891 777 762 696 693 490
467 449 346 335 304 302 246 121
86 23 13 12
BstZ17I GTA'TAC
Cuts at: 1949 1949
Size: 9644
Bsu36I CC'TnA_GG
Cuts at: 185 5296 185
Size: 5111 4533
BtgI C'CryG_G
Cuts at: 107 157 4769 5993 107
Size: 50 4612 1224 3758
Fragments arranged by size:
4612 3758 1224 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1749 3312 3498 4236 7594 7775 7938 1749
Size: 1563 186 738 3358 181 163 3455
Fragments arranged by size:
3455 3358 1563 738 186 181 163
BtsI GCAGTG_nn'
Cuts at: 285 808 2579 3529 3885 5026 5329 5335 6166
Size: 523 1771 950 356 1141 303 6 831
Cuts at: 6166 6887 285
Size: 721 3042
Fragments arranged by size:
3042 1771 1141 950 831 721 523 356
303 6
ClaI AT'CG_AT
Cuts at: 5081 5081
Size: 9644
DraI TTT'AAA
Cuts at: 3062 6643 3062
Size: 3581 6063
DraIII CAC_nnn'GTG
Cuts at: 3373 4775 3373
Size: 1402 8242
DrdI GACnn_nn'nnGTC
Cuts at: 7350 8208 7350
Size: 858 8786
EaeI y'GGCC_r
Cuts at: 154 724 824 3900 7229 7403 7794 7821 9166
Size: 570 100 3076 3329 174 391 27 1345
Cuts at: 9166 154
Size: 632
Fragments arranged by size:
3329 3076 1345 632 570 391 174 100
27
EagI C'GGCC_G
Cuts at: 154 7229 154
Size: 7075 2569
EarI CTCTTCn'nnn_
Cuts at: 521 631 1693 3088 4491 4513 4846 5913 7667
Size: 110 1062 1395 1403 22 333 1067 1754
Cuts at: 7667 7877 521
Size: 210 2288
Fragments arranged by size:
2288 1754 1403 1395 1067 1062 333 210
110 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 892 1723 7852 8162 8308 9454 892
Size: 831 6129 310 146 1146 1082
Fragments arranged by size:
6129 1146 1082 831 310 146
EcoICRI GAG'CTC
Cuts at: 3081 4839 6656 3081
Size: 1758 1817 6069
Fragments arranged by size:
6069 1817 1758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 309 929 1509 1714 2075 2531 2588 2606
Size: 140 620 580 205 361 456 57 18
Cuts at: 2606 2686 3069 4135 4813 5344 5381 5753 5836
Size: 80 383 1066 678 531 37 372 83
Cuts at: 5836 5997 6517 7469 7901 8648 8916 169
Size: 161 520 952 432 747 268 897
Fragments arranged by size:
1066 952 897 747 678 620 580 531
520 456 432 383 372 361 268 205
161 140 83 80 57 37 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2926 3695 2926
Size: 769 8875
EcoO109I rG'GnC_Cy
Cuts at: 166 189 504 730 766 767 1570 1974 2267
Size: 23 315 226 36 1 803 404 293
Cuts at: 2267 3611 5300 5980 6114 6783 166
Size: 1344 1689 680 134 669 3027
Fragments arranged by size:
3027 1689 1344 803 680 669 404 315
293 226 134 36 23 1
EcoRI G'AATT_C
Cuts at: 1882 6648 1882
Size: 4766 4878
EcoRV GAT'ATC
Cuts at: 2439 2592 2439
Size: 153 9491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 426 2287 2457 7663 426
Size: 1861 170 5206 2407
Fragments arranged by size:
5206 2407 1861 170
FspI TGC'GCA
Cuts at: 3958 7425 3958
Size: 3467 6177
HaeII r_GCGC'y
Cuts at: 3015 4065 4374 4989 5838 6525 7078 7326 8348
Size: 1050 309 615 849 687 553 248 1022
Cuts at: 8348 9327 3015
Size: 979 3332
Fragments arranged by size:
3332 1050 1022 979 849 687 615 553
309 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1842 1874 1918 1950 2986 3018 3082 3114 3165
Size: 32 44 32 1036 32 64 32 51
Cuts at: 3165 3197 3912 3944 4282 4314 4810 4842 4975
Size: 32 715 32 338 32 496 32 133
Cuts at: 4975 5007 5036 5068 5541 5573 6129 6161 7486
Size: 32 29 32 473 32 556 32 1325
Cuts at: 7486 7516 7518 7548 1842
Size: 30 2 30 3938
Fragments arranged by size:
3938 1325 1036 715 556 496 473 338
133 64 51 44 32 32 32 32
32 32 32 32 32 32 32 32
30 30 29 2
HincII GTy'rAC
Cuts at: 648 1079 4818 6683 648
Size: 431 3739 1865 3609
Fragments arranged by size:
3739 3609 1865 431
KasI G'GCGC_C
Cuts at: 7074 7322 7074
Size: 248 9396
KpnI G_GTAC'C
Cuts at: 5993 6664 5993
Size: 671 8973
MluI A'CGCG_T
Cuts at: 9077 9077
Size: 9644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 344 1337 3868 4302 4835 4853 5509 8315 8499
Size: 993 2531 434 533 18 656 2806 184
Cuts at: 8499 344
Size: 1489
Fragments arranged by size:
2806 2531 1489 993 656 533 434 184
18
MscI TGG'CCA
Cuts at: 826 7405 826
Size: 6579 3065
MslI CAynn'nnrTG
Cuts at: 278 589 832 1626 2906 3290 3913 4849 5749
Size: 311 243 794 1280 384 623 936 900
Cuts at: 5749 7633 7760 278
Size: 1884 127 2162
Fragments arranged by size:
2162 1884 1280 936 900 794 623 384
311 243 127
MspA1I CmG'CkG
Cuts at: 159 1032 1196 1646 2364 2793 4128 4808 5019
Size: 873 164 450 718 429 1335 680 211
Cuts at: 5019 5271 6087 6755 7069 7429 8442 8687 159
Size: 252 816 668 314 360 1013 245 1116
Fragments arranged by size:
1335 1116 1013 873 816 718 680 668
450 429 360 314 252 245 211 164
NaeI GCC'GGC
Cuts at: 994 7825 994
Size: 6831 2813
NarI GG'CG_CC
Cuts at: 7075 7323 7075
Size: 248 9396
NcoI C'CATG_G
Cuts at: 107 5993 107
Size: 5886 3758
NgoMIV G'CCGG_C
Cuts at: 992 7823 992
Size: 6831 2813
NotI GC'GGCC_GC
Cuts at: 154 154
Size: 9644
NsiI A_TGCA'T
Cuts at: 4697 4697
Size: 9644
NspI r_CATG'y
Cuts at: 646 1947 2697 2725 4329 4435 4527 4571 4737
Size: 1301 750 28 1604 106 92 44 166
Cuts at: 4737 4824 4863 4902 5871 6697 7197 8104 646
Size: 87 39 39 969 826 500 907 2186
Fragments arranged by size:
2186 1604 1301 969 907 826 750 500
166 106 92 87 44 39 39 28
PasI CC'CwG_GG
Cuts at: 5168 5931 6213 5168
Size: 763 282 8599
Fragments arranged by size:
8599 763 282
PciI A'CATG_T
Cuts at: 642 1943 4431 4567 4898 8100 642
Size: 1301 2488 136 331 3202 2186
Fragments arranged by size:
3202 2488 2186 1301 331 136
PflMI CCAn_nnn'nTGG
Cuts at: 2367 3729 4158 4775 5584 2367
Size: 1362 429 617 809 6427
Fragments arranged by size:
6427 1362 809 617 429
PfoI T'CCnGG_A
Cuts at: 1691 1805 2508 2653 3533 4790 1691
Size: 114 703 145 880 1257 6545
Fragments arranged by size:
6545 1257 880 703 145 114
PmeI GTTT'AAAC
Cuts at: 6643 6643
Size: 9644
PmlI CAC'GTG
Cuts at: 2301 5057 2301
Size: 2756 6888
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8783 8815 8783
Size: 32 9612
PpuMI rG'GwC_Cy
Cuts at: 189 504 1570 1974 2267 189
Size: 315 1066 404 293 7566
Fragments arranged by size:
7566 1066 404 315 293
PshAI GACnn'nnGTC
Cuts at: 374 5758 374
Size: 5384 4260
PsiI TTA'TAA
Cuts at: 5786 5786
Size: 9644
PspOMI G'GGCC_C
Cuts at: 766 3611 5520 766
Size: 2845 1909 4890
Fragments arranged by size:
4890 2845 1909
PspXI vC'TCGA_Gb
Cuts at: 5252 5252
Size: 9644
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3103 3135 3103
Size: 32 9612
PstI C_TGCA'G
Cuts at: 738 1031 1455 1823 3009 3198 4199 4896 5658
Size: 293 424 368 1186 189 1001 697 762
Cuts at: 5658 5988 6173 6691 7376 738
Size: 330 185 518 685 3006
Fragments arranged by size:
3006 1186 1001 762 697 685 518 424
368 330 293 189 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9644
PvuII CAG'CTG
Cuts at: 1032 1196 1646 2793 5019 7069 7429 1032
Size: 164 450 1147 2226 2050 360 3247
Fragments arranged by size:
3247 2226 2050 1147 450 360 164
RsrII CG'GwC_CG
Cuts at: 2990 7839 2990
Size: 4849 4795
SacI G_AGCT'C
Cuts at: 3083 4841 6658 3083
Size: 1758 1817 6069
Fragments arranged by size:
6069 1817 1758
SacII CC_GC'GG
Cuts at: 160 160
Size: 9644
SalI G'TCGA_C
Cuts at: 4816 6681 4816
Size: 1865 7779
SanDI GG'GwC_CC
Cuts at: 2267 2267
Size: 9644
SapI GCTCTTCn'nnn_
Cuts at: 3088 4513 4846 5913 7667 7877 3088
Size: 1425 333 1067 1754 210 4855
Fragments arranged by size:
4855 1754 1425 1067 333 210
SbfI CC_TGCA'GG
Cuts at: 738 5658 5988 6691 738
Size: 4920 330 703 3691
Fragments arranged by size:
4920 3691 703 330
ScaI AGT'ACT
Cuts at: 685 1898 4314 4663 5442 9161 685
Size: 1213 2416 349 779 3719 1168
Fragments arranged by size:
3719 2416 1213 1168 779 349
SexAI A'CCwGG_T
Cuts at: 2633 4721 2633
Size: 2088 7556
SfcI C'TryA_G
Cuts at: 33 734 1027 1204 1451 1819 3005 3051 3194
Size: 701 293 177 247 368 1186 46 143
Cuts at: 3194 3258 3692 4195 4617 4892 5137 5607 5654
Size: 64 434 503 422 275 245 470 47
Cuts at: 5654 5984 6169 6687 7372 8365 8556 33
Size: 330 185 518 685 993 191 1121
Fragments arranged by size:
1186 1121 993 701 685 518 503 470
434 422 368 330 293 275 247 245
191 185 177 143 64 47 46
SfoI GGC'GCC
Cuts at: 7076 7324 7076
Size: 248 9396
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9644
SmaI CCC'GGG
Cuts at: 5525 6666 5525
Size: 1141 8503
SmlI C'TyrA_G
Cuts at: 126 883 3394 3778 3997 5252 6799 7714 8206
Size: 757 2511 384 219 1255 1547 915 492
Cuts at: 8206 8468 126
Size: 262 1302
Fragments arranged by size:
2511 1547 1302 1255 915 757 492 384
262 219
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9644
SphI G_CATG'C
Cuts at: 6697 7197 6697
Size: 500 9144
SspI AAT'ATT
Cuts at: 2752 2752
Size: 9644
StuI AGG'CCT
Cuts at: 3400 5627 3400
Size: 2227 7417
StyI C'CwwG_G
Cuts at: 107 626 1235 2249 2271 2432 2688 4187 4256
Size: 519 609 1014 22 161 256 1499 69
Cuts at: 4256 5087 5468 5826 5993 6778 7132 107
Size: 831 381 358 167 785 354 2619
Fragments arranged by size:
2619 1499 1014 831 785 609 519 381
358 354 256 167 161 69 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 867 2262 2329 4774 5451 5591 5650 6049 6195
Size: 1395 67 2445 677 140 59 399 146
Cuts at: 6195 7227 7360 7819 8028 867
Size: 1032 133 459 209 2483
Fragments arranged by size:
2483 2445 1395 1032 677 459 399 209
146 140 133 67 59
TatI w'GTAC_w
Cuts at: 683 1009 1896 3041 3792 4312 4661 5440 5630
Size: 326 887 1145 751 520 349 779 190
Cuts at: 5630 9159 683
Size: 3529 1168
Fragments arranged by size:
3529 1168 1145 887 779 751 520 349
326 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1089 1121 2304 2336 3882 3914 4465 4497 9161
Size: 32 1183 32 1546 32 551 32 4664
Cuts at: 9161 9193 1089
Size: 32 1540
Fragments arranged by size:
4664 1546 1540 1183 551 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4296 7441 4296
Size: 3145 6499
XbaI T'CTAG_A
Cuts at: 3183 4365 6675 3183
Size: 1182 2310 6152
Fragments arranged by size:
6152 2310 1182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4788 5581 7139 4788
Size: 793 1558 7293
Fragments arranged by size:
7293 1558 793
XhoI C'TCGA_G
Cuts at: 5252 5252
Size: 9644
XmaI C'CCGG_G
Cuts at: 5523 6664 5523
Size: 1141 8503
XmnI GAAnn'nnTTC
Cuts at: 968 1916 3851 5009 968
Size: 948 1935 1158 5603
Fragments arranged by size:
5603 1935 1158 948
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AloI AscI AvrII BmtI BsiWI BsmBI BssHII
BstEII CspCI FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI PacI SfiI SgrAI SnaBI SrfI SwaI
ZraI