Restriction map for
pF1KE2271
(Circular) (Six-base) MAPSORT of: pF1KE2271.seq Check: 9749 from: 1 to: 4487
pF1KE2271 4487 bp
With 182 enzymes: SgfI *
November 2, 2010 17:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 512 512
Size: 4487
AccI GT'mk_AC
Cuts at: 1525 1525
Size: 4487
Acc65I G'GTAC_C
Cuts at: 1503 1503
Size: 4487
AclI AA'CG_TT
Cuts at: 4327 4327
Size: 4487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 895 2312 2744 3491 3759 493
Size: 402 1417 432 747 268 1221
Fragments arranged by size:
1417 1221 747 432 402 268
AflII C'TTAA_G
Cuts at: 403 403
Size: 4487
AflIII A'CryG_T
Cuts at: 1468 1839 2943 3920 1468
Size: 371 1104 977 2035
Fragments arranged by size:
2035 1104 977 371
AgeI A'CCGG_T
Cuts at: 1454 2875 1454
Size: 1421 3066
AleI CACnn'nnGTG
Cuts at: 1285 1285
Size: 4487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 4487
AlwNI CAG_nnn'CTG
Cuts at: 522 1218 1242 1792 3359 522
Size: 696 24 550 1567 1650
Fragments arranged by size:
1650 1567 696 550 24
ApaLI G'TGCA_C
Cuts at: 3257 3257
Size: 4487
ApoI r'AATT_y
Cuts at: 1080 1491 3840 4096 1080
Size: 411 2349 256 1471
Fragments arranged by size:
2349 1471 411 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4487
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
AvaI C'yCGr_G
Cuts at: 1230 1507 1230
Size: 277 4210
BamHI G'GATC_C
Cuts at: 1512 1512
Size: 4487
BanI G'GyrC_C
Cuts at: 646 1370 1376 1385 1503 1917 2165 2200 646
Size: 724 6 9 118 414 248 35 2933
Fragments arranged by size:
2933 724 414 248 118 35 9 6
BanII G_rGCy'C
Cuts at: 307 544 635 1501 2531 307
Size: 237 91 866 1030 2263
Fragments arranged by size:
2263 1030 866 237 91
BbeI G_GCGC'C
Cuts at: 1389 1921 2169 1389
Size: 532 248 3707
Fragments arranged by size:
3707 532 248
BbsI GAAGACnn'nnnn_
Cuts at: 419 613 1441 1471 419
Size: 194 828 30 3435
Fragments arranged by size:
3435 828 194 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1142 1176 1142
Size: 34 4453
BciVI GTATCCnnnnn_n'
Cuts at: 204 2385 3146 4487 204
Size: 2181 761 1341 204
Fragments arranged by size:
2181 1341 761 204
BclI T'GATC_A
Cuts at: 389 2007 389
Size: 1618 2869
BglII A'GATC_T
Cuts at: 2002 4039 2002
Size: 2037 2450
BlpI GC'TnA_GC
Cuts at: 1126 1599 1126
Size: 473 4014
Bme1580I G_kGCm'C
Cuts at: 1450 2112 2205 3261 1450
Size: 662 93 1056 2676
Fragments arranged by size:
2676 1056 662 93
BmrI ACTGGGnnnn_n'
Cuts at: 1699 1780 2115 4259 1699
Size: 81 335 2144 1927
Fragments arranged by size:
2144 1927 335 81
BmtI G_CTAG'C
Cuts at: 715 715
Size: 4487
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1082 2854 1082
Size: 1772 2715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 649 714 907 1057 1159 619
Size: 30 65 193 150 102 3947
Fragments arranged by size:
3947 193 150 102 65 30
Bpu10I CC'TnA_GC
Cuts at: 917 1115 917
Size: 198 4289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 336 1663 2542 3034 3332 336
Size: 1327 879 492 298 1491
Fragments arranged by size:
1491 1327 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1164 4191 1164
Size: 3027 1460
BsaAI yAC'GTr
Cuts at: 1840 1840
Size: 4487
BsaBI GATnn'nnATC
Cuts at: 6 2001 4038 6
Size: 1995 2037 455
Fragments arranged by size:
2037 1995 455
BsaHI Gr'CG_yC
Cuts at: 1319 1386 1918 2166 2868 4364 1319
Size: 67 532 248 702 1496 1442
Fragments arranged by size:
1496 1442 702 532 248 67
BsaWI w'CCGG_w
Cuts at: 1454 1676 2197 2875 3149 3296 3707 1454
Size: 222 521 678 274 147 411 2234
Fragments arranged by size:
2234 678 521 411 274 222 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 143 173 143
Size: 30 4457
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 469 837 1411 151
Size: 318 368 574 3227
Fragments arranged by size:
3227 574 368 318
BseYI C'CCAG_C
Cuts at: 165 195 1311 1912 2670 3247 3743 165
Size: 30 1116 601 758 577 496 909
Fragments arranged by size:
1116 909 758 601 577 496 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1309 1309
Size: 4487
BsiEI CG_ry'CG
Cuts at: 104 2029 2075 3283 104
Size: 1925 46 1208 1308
Fragments arranged by size:
1925 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 635 1501 2279 2469 3261 635
Size: 866 778 190 792 1861
Fragments arranged by size:
1861 866 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 307 544 635 1450 1501 2112 2205 2279 2469
Size: 237 91 815 51 611 93 74 190
Cuts at: 2469 2531 3261 307
Size: 62 730 1533
Fragments arranged by size:
1533 815 730 611 237 190 93 91
74 62 51
BspEI T'CCGG_A
Cuts at: 3707 3707
Size: 4487
BspHI T'CATG_A
Cuts at: 3663 3663
Size: 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 512 1447 1537 1751 2053 2434 512
Size: 935 90 214 302 381 2565
Fragments arranged by size:
2565 935 381 302 214 90
BsrBI CCG'CTC
Cuts at: 50 945 1398 2779 2833 4317 4482 50
Size: 895 453 1381 54 1484 165 55
Fragments arranged by size:
1484 1381 895 453 165 55 54
BsrDI GCAATG_nn'
Cuts at: 574 2399 574
Size: 1825 2662
BsrFI r'CCGG_y
Cuts at: 1324 1373 1454 2485 2666 2875 1324
Size: 49 81 1031 181 209 2936
Fragments arranged by size:
2936 1031 209 181 81 49
BsrGI T'GTAC_A
Cuts at: 229 229
Size: 4487
BssSI C'ACGA_G
Cuts at: 2758 3116 2758
Size: 358 4129
BstAPI GCAn_nnn'nTGC
Cuts at: 1218 1864 1218
Size: 646 3841
BstBI TT'CG_AA
Cuts at: 2848 2848
Size: 4487
BstEII G'GTnAC_C
Cuts at: 903 903
Size: 4487
BstXI CCAn_nnnn'nTGG
Cuts at: 643 643
Size: 4487
BstYI r'GATC_y
Cuts at: 1 1512 1979 2002 2337 2583 3595 3681 3693
Size: 1511 467 23 335 246 1012 86 12
Cuts at: 3693 4039 1
Size: 346 449
Fragments arranged by size:
1511 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1196 1196
Size: 4487
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2437 2618 2781 2437
Size: 181 163 4143
Fragments arranged by size:
4143 181 163
BtsI GCAGTG_nn'
Cuts at: 816 1219 1730 816
Size: 403 511 3573
Fragments arranged by size:
3573 511 403
DraI TTT'AAA
Cuts at: 1486 1486
Size: 4487
DrdI GACnn_nn'nnGTC
Cuts at: 2193 3051 2193
Size: 858 3629
EaeI y'GGCC_r
Cuts at: 2072 2246 2637 2664 4009 2072
Size: 174 391 27 1345 2550
Fragments arranged by size:
2550 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2072 2072
Size: 4487
EarI CTCTTCn'nnn_
Cuts at: 241 2510 2720 241
Size: 2269 210 2008
Fragments arranged by size:
2269 2008 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 847 2695 3005 3151 4297 847
Size: 1848 310 146 1146 1037
Fragments arranged by size:
1848 1146 1037 310 146
EcoICRI GAG'CTC
Cuts at: 633 1499 633
Size: 866 3621
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 619 649 714 895 907 1057 1159 2312
Size: 126 30 65 181 12 150 102 1153
Cuts at: 2312 2744 3491 3759 493
Size: 432 747 268 1221
Fragments arranged by size:
1221 1153 747 432 268 181 150 126
102 65 30 12
EcoNI CCTnn'n_nnAGG
Cuts at: 508 961 508
Size: 453 4034
EcoO109I rG'GnC_Cy
Cuts at: 912 1102 1239 1626 912
Size: 190 137 387 3773
Fragments arranged by size:
3773 387 190 137
EcoRI G'AATT_C
Cuts at: 1491 1491
Size: 4487
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4487
FspI TGC'GCA
Cuts at: 2268 2268
Size: 4487
HaeII r_GCGC'y
Cuts at: 160 1389 1921 2169 3191 4170 160
Size: 1229 532 248 1022 979 477
Fragments arranged by size:
1229 1022 979 532 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1387 1419 2329 2359 2361 2391 1387
Size: 32 910 30 2 30 3483
Fragments arranged by size:
3483 910 32 30 30 2
HincII GTy'rAC
Cuts at: 1526 1526
Size: 4487
KasI G'GCGC_C
Cuts at: 1385 1917 2165 1385
Size: 532 248 3707
Fragments arranged by size:
3707 532 248
KpnI G_GTAC'C
Cuts at: 1507 1507
Size: 4487
MfeI C'AATT_G
Cuts at: 463 463
Size: 4487
MluI A'CGCG_T
Cuts at: 3920 3920
Size: 4487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 1277 3158 3342 299
Size: 978 1881 184 1444
Fragments arranged by size:
1881 1444 978 184
MscI TGG'CCA
Cuts at: 2248 2248
Size: 4487
MslI CAynn'nnrTG
Cuts at: 706 1285 2476 2603 706
Size: 579 1191 127 2590
Fragments arranged by size:
2590 1191 579 127
MspA1I CmG'CkG
Cuts at: 627 1598 1912 2272 3285 3530 627
Size: 971 314 360 1013 245 1584
Fragments arranged by size:
1584 1013 971 360 314 245
NaeI GCC'GGC
Cuts at: 1326 1375 2668 1326
Size: 49 1293 3145
Fragments arranged by size:
3145 1293 49
NarI GG'CG_CC
Cuts at: 1386 1918 2166 1386
Size: 532 248 3707
Fragments arranged by size:
3707 532 248
NgoMIV G'CCGG_C
Cuts at: 1324 1373 2666 1324
Size: 49 1293 3145
Fragments arranged by size:
3145 1293 49
NheI G'CTAG_C
Cuts at: 711 711
Size: 4487
NspI r_CATG'y
Cuts at: 659 1472 1540 2040 2947 659
Size: 813 68 500 907 2199
Fragments arranged by size:
2199 907 813 500 68
PciI A'CATG_T
Cuts at: 1468 2943 1468
Size: 1475 3012
PflMI CCAn_nnn'nTGG
Cuts at: 857 857
Size: 4487
PfoI T'CCnGG_A
Cuts at: 635 635
Size: 4487
PmeI GTTT'AAAC
Cuts at: 1486 1486
Size: 4487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4455
PpuMI rG'GwC_Cy
Cuts at: 912 1102 1239 912
Size: 190 137 4160
Fragments arranged by size:
4160 190 137
PstI C_TGCA'G
Cuts at: 1534 2219 1534
Size: 685 3802
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4487
PvuII CAG'CTG
Cuts at: 627 1912 2272 627
Size: 1285 360 2842
Fragments arranged by size:
2842 1285 360
RsrII CG'GwC_CG
Cuts at: 2682 2682
Size: 4487
SacI G_AGCT'C
Cuts at: 635 1501 635
Size: 866 3621
SalI G'TCGA_C
Cuts at: 1524 1524
Size: 4487
SanDI GG'GwC_CC
Cuts at: 1102 1102
Size: 4487
SapI GCTCTTCn'nnn_
Cuts at: 241 2510 2720 241
Size: 2269 210 2008
Fragments arranged by size:
2269 2008 210
SbfI CC_TGCA'GG
Cuts at: 1534 1534
Size: 4487
ScaI AGT'ACT
Cuts at: 4004 4004
Size: 4487
SexAI A'CCwGG_T
Cuts at: 1046 1046
Size: 4487
SfcI C'TryA_G
Cuts at: 33 1530 2215 3208 3399 33
Size: 1497 685 993 191 1121
Fragments arranged by size:
1497 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1387 1919 2167 1387
Size: 532 248 3707
Fragments arranged by size:
3707 532 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
SmaI CCC'GGG
Cuts at: 1509 1509
Size: 4487
SmlI C'TyrA_G
Cuts at: 351 403 1642 2557 3049 3311 351
Size: 52 1239 915 492 262 1527
Fragments arranged by size:
1527 1239 915 492 262 52
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4487
SphI G_CATG'C
Cuts at: 1540 2040 1540
Size: 500 3987
StuI AGG'CCT
Cuts at: 727 967 727
Size: 240 4247
StyI C'CwwG_G
Cuts at: 372 533 580 1621 1975 372
Size: 161 47 1041 354 2884
Fragments arranged by size:
2884 1041 354 161 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 514 1173 2070 2203 2662 2871 514
Size: 659 897 133 459 209 2130
Fragments arranged by size:
2130 897 659 459 209 133
TatI w'GTAC_w
Cuts at: 229 4002 229
Size: 3773 714
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 173 205 4004 4036 173
Size: 32 3799 32 624
Fragments arranged by size:
3799 624 32 32
Tth111I GACn'n_nGTC
Cuts at: 2284 2284
Size: 4487
XbaI T'CTAG_A
Cuts at: 1518 1518
Size: 4487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1982 1982
Size: 4487
XmaI C'CCGG_G
Cuts at: 1507 1507
Size: 4487
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AloI ApaI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI BsiWI BsmI BsmBI BssHII
BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NcoI NdeI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI