Restriction map for
pF1KE2237
(Circular) (Six-base) MAPSORT of: pF1KE2237.seq Check: 2201 from: 1 to: 5192
pF1KE2237 5192 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 915 915
Size: 5192
AatII G_ACGT'C
Cuts at: 118 118
Size: 5192
AccI GT'mk_AC
Cuts at: 2230 2230
Size: 5192
Acc65I G'GTAC_C
Cuts at: 2208 2208
Size: 5192
AclI AA'CG_TT
Cuts at: 5032 5032
Size: 5192
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1007 1180 1865 3017 3449 4196 4464 808
Size: 199 173 685 1152 432 747 268 1536
Fragments arranged by size:
1536 1152 747 685 432 268 199 173
AflIII A'CryG_T
Cuts at: 2544 3648 4625 2544
Size: 1104 977 3111
Fragments arranged by size:
3111 1104 977
AgeI A'CCGG_T
Cuts at: 3580 3580
Size: 5192
AleI CACnn'nnGTG
Cuts at: 1391 1391
Size: 5192
AlwNI CAG_nnn'CTG
Cuts at: 1458 2044 2497 4064 1458
Size: 586 453 1567 2586
Fragments arranged by size:
2586 1567 586 453
ApaI G_GGCC'C
Cuts at: 1087 1429 1724 1087
Size: 342 295 4555
Fragments arranged by size:
4555 342 295
ApaLI G'TGCA_C
Cuts at: 3962 3962
Size: 5192
ApoI r'AATT_y
Cuts at: 169 2196 4545 4801 169
Size: 2027 2349 256 560
Fragments arranged by size:
2349 2027 560 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5192
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5192
AvaI C'yCGr_G
Cuts at: 2212 2212
Size: 5192
BamHI G'GATC_C
Cuts at: 1827 1893 2217 1827
Size: 66 324 4802
Fragments arranged by size:
4802 324 66
BanI G'GyrC_C
Cuts at: 404 1342 2208 2622 2870 2905 404
Size: 938 866 414 248 35 2691
Fragments arranged by size:
2691 938 866 414 248 35
BanII G_rGCy'C
Cuts at: 301 367 928 1087 1204 1429 1724 1807 2206
Size: 66 561 159 117 225 295 83 399
Cuts at: 2206 3236 301
Size: 1030 2257
Fragments arranged by size:
2257 1030 561 399 295 225 159 117
83 66
BbeI G_GCGC'C
Cuts at: 1346 2626 2874 1346
Size: 1280 248 3664
Fragments arranged by size:
3664 1280 248
BbsI GAAGACnn'nnnn_
Cuts at: 1490 1490
Size: 5192
BbvCI CC'TCA_GC
Cuts at: 1184 1402 1184
Size: 218 4974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 568 602 568
Size: 34 5158
BciVI GTATCCnnnnn_n'
Cuts at: 657 1518 3090 3851 5192 657
Size: 861 1572 761 1341 657
Fragments arranged by size:
1572 1341 861 761 657
BclI T'GATC_A
Cuts at: 2712 2712
Size: 5192
BglI GCCn_nnn'nGGC
Cuts at: 1728 1728
Size: 5192
BglII A'GATC_T
Cuts at: 2707 4744 2707
Size: 2037 3155
BlpI GC'TnA_GC
Cuts at: 2304 2304
Size: 5192
Bme1580I G_kGCm'C
Cuts at: 1087 1400 1429 1724 2136 2817 2910 3966 1087
Size: 313 29 295 412 681 93 1056 2313
Fragments arranged by size:
2313 1056 681 412 313 295 93 29
BmgBI CAC'GTC
Cuts at: 709 709
Size: 5192
BmrI ACTGGGnnnn_n'
Cuts at: 647 1718 2104 2404 2485 2820 4964 647
Size: 1071 386 300 81 335 2144 875
Fragments arranged by size:
2144 1071 875 386 335 300 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1199 3559 1199
Size: 2360 2832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 1804 1964 1147
Size: 657 160 4375
Fragments arranged by size:
4375 657 160
Bpu10I CC'TnA_GC
Cuts at: 1147 1174 1184 1402 1944 1147
Size: 27 10 218 542 4395
Fragments arranged by size:
4395 542 218 27 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2368 3247 3739 4037 2368
Size: 879 492 298 3523
Fragments arranged by size:
3523 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 320 4896 320
Size: 4576 616
BsaAI yAC'GTr
Cuts at: 2545 2545
Size: 5192
BsaBI GATnn'nnATC
Cuts at: 6 244 2706 4743 6
Size: 238 2462 2037 455
Fragments arranged by size:
2462 2037 455 238
BsaHI Gr'CG_yC
Cuts at: 115 1343 1436 1514 2623 2871 3573 5069 115
Size: 1228 93 78 1109 248 702 1496 238
Fragments arranged by size:
1496 1228 1109 702 248 238 93 78
BsaWI w'CCGG_w
Cuts at: 2381 2902 3580 3854 4001 4412 2381
Size: 521 678 274 147 411 3161
Fragments arranged by size:
3161 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 235 265 1012 1042 1314 1344 235
Size: 30 747 30 272 30 4083
Fragments arranged by size:
4083 747 272 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 244 506 772 1221 1290 1293 1296 1305
Size: 18 262 266 449 69 3 3 9
Cuts at: 1305 1308 1965 2173 226
Size: 3 657 208 3245
Fragments arranged by size:
3245 657 449 266 262 208 69 18
9 3 3 3
BseYI C'CCAG_C
Cuts at: 688 700 1190 1428 1598 2617 3375 3952 4448
Size: 12 490 238 170 1019 758 577 496
Cuts at: 4448 688
Size: 1432
Fragments arranged by size:
1432 1019 758 577 496 490 238 170
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 584 934 1678 448
Size: 136 350 744 3962
Fragments arranged by size:
3962 744 350 136
BsiEI CG_ry'CG
Cuts at: 104 2734 2780 3988 104
Size: 2630 46 1208 1308
Fragments arranged by size:
2630 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 301 400 1290 2206 2984 3174 3966 301
Size: 99 890 916 778 190 792 1527
Fragments arranged by size:
1527 916 890 792 778 190 99
BsmBI CGTCTCn'nnnn_
Cuts at: 208 208
Size: 5192
Bsp1286I G_dGCh'C
Cuts at: 301 367 400 928 1087 1204 1290 1400 1429
Size: 66 33 528 159 117 86 110 29
Cuts at: 1429 1724 1807 2136 2206 2817 2910 2984 3174
Size: 295 83 329 70 611 93 74 190
Cuts at: 3174 3236 3966 301
Size: 62 730 1527
Fragments arranged by size:
1527 730 611 528 329 295 190 159
117 110 93 86 83 74 70 66
62 33 29
BspEI T'CCGG_A
Cuts at: 4412 4412
Size: 5192
BspHI T'CATG_A
Cuts at: 4368 4368
Size: 5192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 756 915 956 2242 2456 2758 3139 756
Size: 159 41 1286 214 302 381 2809
Fragments arranged by size:
2809 1286 381 302 214 159 41
BsrBI CCG'CTC
Cuts at: 50 3484 3538 5022 5187 50
Size: 3434 54 1484 165 55
Fragments arranged by size:
3434 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3104 3104
Size: 5192
BsrFI r'CCGG_y
Cuts at: 1751 2177 3190 3371 3580 1751
Size: 426 1013 181 209 3363
Fragments arranged by size:
3363 1013 426 209 181
BssSI C'ACGA_G
Cuts at: 3463 3821 3463
Size: 358 4834
BstAPI GCAn_nnn'nTGC
Cuts at: 2569 2569
Size: 5192
BstBI TT'CG_AA
Cuts at: 3553 3553
Size: 5192
BstEII G'GTnAC_C
Cuts at: 1553 1553
Size: 5192
BstXI CCAn_nnnn'nTGG
Cuts at: 978 1266 978
Size: 288 4904
BstYI r'GATC_y
Cuts at: 1 435 1827 1893 2217 2684 2707 3042 3288
Size: 434 1392 66 324 467 23 335 246
Cuts at: 3288 4300 4386 4398 4744 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1392 1012 467 449 434 346 335 324
246 86 66 23 12
Bsu36I CC'TnA_GG
Cuts at: 1665 1665
Size: 5192
BtgI C'CryG_G
Cuts at: 107 143 408 793 1938 107
Size: 36 265 385 1145 3361
Fragments arranged by size:
3361 1145 385 265 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3142 3323 3486 3142
Size: 181 163 4848
Fragments arranged by size:
4848 181 163
BtsI GCAGTG_nn'
Cuts at: 964 2178 2435 964
Size: 1214 257 3721
Fragments arranged by size:
3721 1214 257
DraI TTT'AAA
Cuts at: 2191 2191
Size: 5192
DraIII CAC_nnn'GTG
Cuts at: 1285 1285
Size: 5192
DrdI GACnn_nn'nnGTC
Cuts at: 2898 3756 2898
Size: 858 4334
EaeI y'GGCC_r
Cuts at: 140 350 1753 2012 2777 2951 3342 3369 4714
Size: 210 1403 259 765 174 391 27 1345
Cuts at: 4714 140
Size: 618
Fragments arranged by size:
1403 1345 765 618 391 259 210 174
27
EagI C'GGCC_G
Cuts at: 2777 2777
Size: 5192
EarI CTCTTCn'nnn_
Cuts at: 828 983 1314 3215 3425 828
Size: 155 331 1901 210 2595
Fragments arranged by size:
2595 1901 331 210 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3400 3710 3856 5002 3400
Size: 310 146 1146 3590
Fragments arranged by size:
3590 1146 310 146
EcoICRI GAG'CTC
Cuts at: 299 2204 299
Size: 1905 3287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1007 1147 1180 1804 1865 1964 3017 3449
Size: 199 140 33 624 61 99 1053 432
Cuts at: 3449 4196 4464 808
Size: 747 268 1536
Fragments arranged by size:
1536 1053 747 624 432 268 199 140
99 61 33
EcoO109I rG'GnC_Cy
Cuts at: 676 1373 1445 1849 1948 2331 676
Size: 697 72 404 99 383 3537
Fragments arranged by size:
3537 697 404 383 99 72
EcoRI G'AATT_C
Cuts at: 2196 2196
Size: 5192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3211 3211
Size: 5192
FspI TGC'GCA
Cuts at: 2083 2973 2083
Size: 890 4302
HaeII r_GCGC'y
Cuts at: 1346 2626 2874 3896 4875 1346
Size: 1280 248 1022 979 1663
Fragments arranged by size:
1663 1280 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 267 1792 1824 3034 3064 3066 3096 235
Size: 32 1525 32 1210 30 2 30 2331
Fragments arranged by size:
2331 1525 1210 32 32 30 30 2
HincII GTy'rAC
Cuts at: 330 2231 330
Size: 1901 3291
HpaI GTT'AAC
Cuts at: 330 330
Size: 5192
KasI G'GCGC_C
Cuts at: 1342 2622 2870 1342
Size: 1280 248 3664
Fragments arranged by size:
3664 1280 248
KpnI G_GTAC'C
Cuts at: 2212 2212
Size: 5192
MluI A'CGCG_T
Cuts at: 4625 4625
Size: 5192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3863 4047 3863
Size: 184 5008
MscI TGG'CCA
Cuts at: 2953 2953
Size: 5192
MslI CAynn'nnrTG
Cuts at: 308 638 1391 3181 3308 308
Size: 330 753 1790 127 2192
Fragments arranged by size:
2192 1790 753 330 127
MspA1I CmG'CkG
Cuts at: 1458 1551 2044 2303 2617 2977 3990 4235 1458
Size: 93 493 259 314 360 1013 245 2415
Fragments arranged by size:
2415 1013 493 360 314 259 245 93
NaeI GCC'GGC
Cuts at: 3373 3373
Size: 5192
NarI GG'CG_CC
Cuts at: 1343 2623 2871 1343
Size: 1280 248 3664
Fragments arranged by size:
3664 1280 248
NcoI C'CATG_G
Cuts at: 107 408 793 1938 107
Size: 301 385 1145 3361
Fragments arranged by size:
3361 1145 385 301
NgoMIV G'CCGG_C
Cuts at: 3371 3371
Size: 5192
NspI r_CATG'y
Cuts at: 2118 2245 2745 3652 2118
Size: 127 500 907 3658
Fragments arranged by size:
3658 907 500 127
PasI CC'CwG_GG
Cuts at: 1266 1369 1266
Size: 103 5089
PciI A'CATG_T
Cuts at: 3648 3648
Size: 5192
PflMI CCAn_nnn'nTGG
Cuts at: 414 1882 414
Size: 1468 3724
PmeI GTTT'AAAC
Cuts at: 2191 2191
Size: 5192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4331 4363 4331
Size: 32 5160
PpuMI rG'GwC_Cy
Cuts at: 1445 1445
Size: 5192
PshAI GACnn'nnGTC
Cuts at: 834 834
Size: 5192
PspOMI G'GGCC_C
Cuts at: 1083 1425 1720 1083
Size: 342 295 4555
Fragments arranged by size:
4555 342 295
PstI C_TGCA'G
Cuts at: 194 570 1139 2239 2924 194
Size: 376 569 1100 685 2462
Fragments arranged by size:
2462 1100 685 569 376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5192
PvuII CAG'CTG
Cuts at: 1458 2044 2617 2977 1458
Size: 586 573 360 3673
Fragments arranged by size:
3673 586 573 360
RsrII CG'GwC_CG
Cuts at: 3387 3387
Size: 5192
SacI G_AGCT'C
Cuts at: 301 2206 301
Size: 1905 3287
SalI G'TCGA_C
Cuts at: 2229 2229
Size: 5192
SapI GCTCTTCn'nnn_
Cuts at: 3215 3425 3215
Size: 210 4982
SbfI CC_TGCA'GG
Cuts at: 2239 2239
Size: 5192
ScaI AGT'ACT
Cuts at: 4709 4709
Size: 5192
SfcI C'TryA_G
Cuts at: 33 190 495 566 1135 1621 2235 2920 3913
Size: 157 305 71 569 486 614 685 993
Cuts at: 3913 4104 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 614 569 486 305 191
157 71
SfoI GGC'GCC
Cuts at: 1344 2624 2872 1344
Size: 1280 248 3664
Fragments arranged by size:
3664 1280 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5192
SmaI CCC'GGG
Cuts at: 2214 2214
Size: 5192
SmlI C'TyrA_G
Cuts at: 2347 3262 3754 4016 2347
Size: 915 492 262 3523
Fragments arranged by size:
3523 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5192
SphI G_CATG'C
Cuts at: 2245 2745 2245
Size: 500 4692
StyI C'CwwG_G
Cuts at: 107 408 793 1235 1938 2326 2680 107
Size: 301 385 442 703 388 354 2619
Fragments arranged by size:
2619 703 442 388 385 354 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 824 1142 1503 1870 2775 2908 3367 3576 824
Size: 318 361 367 905 133 459 209 2440
Fragments arranged by size:
2440 905 459 367 361 318 209 133
TatI w'GTAC_w
Cuts at: 1248 4707 1248
Size: 3459 1733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1314 1346 4709 4741 1314
Size: 32 3363 32 1765
Fragments arranged by size:
3363 1765 32 32
Tth111I GACn'n_nGTC
Cuts at: 2989 2989
Size: 5192
XbaI T'CTAG_A
Cuts at: 2223 2223
Size: 5192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 340 530 1053 1263 1821 2687 340
Size: 190 523 210 558 866 2845
Fragments arranged by size:
2845 866 558 523 210 190
XmaI C'CCGG_G
Cuts at: 2212 2212
Size: 5192
ZraI GAC'GTC
Cuts at: 116 116
Size: 5192
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI