Restriction map for
pF1KE2186
(Circular) (Six-base) MAPSORT of: pF1KE2186.seq Check: 1888 from: 1 to: 4868
pF1KE2186 4868 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1564 1833 1564
Size: 269 4599
AccI GT'mk_AC
Cuts at: 1073 1906 1073
Size: 833 4035
Acc65I G'GTAC_C
Cuts at: 765 1884 765
Size: 1119 3749
AclI AA'CG_TT
Cuts at: 4708 4708
Size: 4868
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 771 2693 3125 3872 4140 727
Size: 44 1922 432 747 268 1455
Fragments arranged by size:
1922 1455 747 432 268 44
AflIII A'CryG_T
Cuts at: 2220 3324 4301 2220
Size: 1104 977 2787
Fragments arranged by size:
2787 1104 977
AgeI A'CCGG_T
Cuts at: 3256 3256
Size: 4868
AleI CACnn'nnGTG
Cuts at: 865 865
Size: 4868
AlwNI CAG_nnn'CTG
Cuts at: 1296 1715 2173 3740 1296
Size: 419 458 1567 2424
Fragments arranged by size:
2424 1567 458 419
ApaI G_GGCC'C
Cuts at: 667 1042 1327 1822 667
Size: 375 285 495 3713
Fragments arranged by size:
3713 495 375 285
ApaLI G'TGCA_C
Cuts at: 307 1050 3638 307
Size: 743 2588 1537
Fragments arranged by size:
2588 1537 743
ApoI r'AATT_y
Cuts at: 1872 4221 4477 1872
Size: 2349 256 2263
Fragments arranged by size:
2349 2263 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4868
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
AvaI C'yCGr_G
Cuts at: 490 639 1566 1600 1777 1888 490
Size: 149 927 34 177 111 3470
Fragments arranged by size:
3470 927 177 149 111 34
BamHI G'GATC_C
Cuts at: 1893 1893
Size: 4868
BanI G'GyrC_C
Cuts at: 219 579 630 750 765 1417 1753 1884 2298
Size: 360 51 120 15 652 336 131 414
Cuts at: 2298 2546 2581 219
Size: 248 35 2506
Fragments arranged by size:
2506 652 414 360 336 248 131 120
51 35 15
BanII G_rGCy'C
Cuts at: 667 692 1042 1246 1327 1822 1882 2912 667
Size: 25 350 204 81 495 60 1030 2623
Fragments arranged by size:
2623 1030 495 350 204 81 60 25
BbeI G_GCGC'C
Cuts at: 583 634 1757 2302 2550 583
Size: 51 1123 545 248 2901
Fragments arranged by size:
2901 1123 545 248 51
BbsI GAAGACnn'nnnn_
Cuts at: 1053 1053
Size: 4868
BciVI GTATCCnnnnn_n'
Cuts at: 2766 3527 4868 2766
Size: 761 1341 2766
Fragments arranged by size:
2766 1341 761
BclI T'GATC_A
Cuts at: 2388 2388
Size: 4868
BglI GCCn_nnn'nGGC
Cuts at: 476 577 588 994 476
Size: 101 11 406 4350
Fragments arranged by size:
4350 406 101 11
BglII A'GATC_T
Cuts at: 2383 4420 2383
Size: 2037 2831
BlpI GC'TnA_GC
Cuts at: 1240 1980 1240
Size: 740 4128
Bme1580I G_kGCm'C
Cuts at: 224 311 667 701 755 1042 1054 1327 1354
Size: 87 356 34 54 287 12 273 27
Cuts at: 1354 1822 2493 2586 3642 224
Size: 468 671 93 1056 1450
Fragments arranged by size:
1450 1056 671 468 356 287 273 93
87 54 34 27 12
BmgBI CAC'GTC
Cuts at: 857 1655 857
Size: 798 4070
BmrI ACTGGGnnnn_n'
Cuts at: 1255 1617 2080 2161 2496 4640 1255
Size: 362 463 81 335 2144 1483
Fragments arranged by size:
2144 1483 463 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3235 3235
Size: 4868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1456 322
Size: 1134 3734
Bpu10I CC'TnA_GC
Cuts at: 908 908
Size: 4868
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 2044 2923 3415 3713 1606
Size: 438 879 492 298 2761
Fragments arranged by size:
2761 879 492 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 1024 4572 1024
Size: 3548 1320
BsaAI yAC'GTr
Cuts at: 1281 1860 2221 1281
Size: 579 361 3928
Fragments arranged by size:
3928 579 361
BsaBI GATnn'nnATC
Cuts at: 6 1453 2382 4419 6
Size: 1447 929 2037 455
Fragments arranged by size:
2037 1447 929 455
BsaHI Gr'CG_yC
Cuts at: 533 580 631 1661 1754 2299 2547 3249 4745
Size: 47 51 1030 93 545 248 702 1496
Cuts at: 4745 533
Size: 656
Fragments arranged by size:
1496 1030 702 656 545 248 93 51
47
BsaWI w'CCGG_w
Cuts at: 2057 2578 3256 3530 3677 4088 2057
Size: 521 678 274 147 411 2837
Fragments arranged by size:
2837 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 522 597 658 954 1210 1320 1538 2293 3051
Size: 75 61 296 256 110 218 755 758
Cuts at: 3051 3628 4124 522
Size: 577 496 1266
Fragments arranged by size:
1266 758 755 577 496 296 256 218
110 75 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1028 1028
Size: 4868
BsiEI CG_ry'CG
Cuts at: 104 182 510 2410 2456 3664 104
Size: 78 328 1900 46 1208 1308
Fragments arranged by size:
1900 1308 1208 328 78 46
BsiHKAI G_wGCw'C
Cuts at: 311 1013 1054 1246 1337 1616 1882 2660 2850
Size: 702 41 192 91 279 266 778 190
Cuts at: 2850 3642 311
Size: 792 1537
Fragments arranged by size:
1537 792 778 702 279 266 192 190
91 41
BsmI GAATG_Cn'
Cuts at: 655 655
Size: 4868
Bsp1286I G_dGCh'C
Cuts at: 224 311 667 692 701 755 1013 1042 1054
Size: 87 356 25 9 54 258 29 12
Cuts at: 1054 1246 1327 1337 1354 1616 1822 1882 2493
Size: 192 81 10 17 262 206 60 611
Cuts at: 2493 2586 2660 2850 2912 3642 224
Size: 93 74 190 62 730 1450
Fragments arranged by size:
1450 730 611 356 262 258 206 192
190 93 87 81 74 62 60 54
29 25 17 12 10 9
BspEI T'CCGG_A
Cuts at: 4088 4088
Size: 4868
BspHI T'CATG_A
Cuts at: 1445 4044 1445
Size: 2599 2269
BspMI ACCTGCnnnn'nnnn_
Cuts at: 359 1349 1564 1833 1918 2132 2434 2815 359
Size: 990 215 269 85 214 302 381 2412
Fragments arranged by size:
2412 990 381 302 269 215 214 85
BsrBI CCG'CTC
Cuts at: 50 425 723 1748 3160 3214 4698 4863 50
Size: 375 298 1025 1412 54 1484 165 55
Fragments arranged by size:
1484 1412 1025 375 298 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2780 2780
Size: 4868
BsrFI r'CCGG_y
Cuts at: 448 633 1138 1267 1579 2866 3047 3256 448
Size: 185 505 129 312 1287 181 209 2060
Fragments arranged by size:
2060 1287 505 312 209 185 181 129
BsrGI T'GTAC_A
Cuts at: 374 374
Size: 4868
BssHII G'CGCG_C
Cuts at: 438 1590 438
Size: 1152 3716
BssSI C'ACGA_G
Cuts at: 3139 3497 3139
Size: 358 4510
BstAPI GCAn_nnn'nTGC
Cuts at: 503 2245 503
Size: 1742 3126
BstBI TT'CG_AA
Cuts at: 3229 3229
Size: 4868
BstXI CCAn_nnnn'nTGG
Cuts at: 867 1497 867
Size: 630 4238
BstYI r'GATC_y
Cuts at: 1 332 1667 1893 2360 2383 2718 2964 3976
Size: 331 1335 226 467 23 335 246 1012
Cuts at: 3976 4062 4074 4420 1
Size: 86 12 346 449
Fragments arranged by size:
1335 1012 467 449 346 335 331 246
226 86 23 12
BtgI C'CryG_G
Cuts at: 107 231 575 1034 1247 1729 1757 107
Size: 124 344 459 213 482 28 3218
Fragments arranged by size:
3218 482 459 344 213 124 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1149 2818 2999 3162 1149
Size: 1669 181 163 2855
Fragments arranged by size:
2855 1669 181 163
BtsI GCAGTG_nn'
Cuts at: 2111 2111
Size: 4868
DraI TTT'AAA
Cuts at: 1867 1867
Size: 4868
DrdI GACnn_nn'nnGTC
Cuts at: 538 1689 2574 3432 538
Size: 1151 885 858 1974
Fragments arranged by size:
1974 1151 885 858
EaeI y'GGCC_r
Cuts at: 179 319 869 1088 1133 1265 1581 1737 1760
Size: 140 550 219 45 132 316 156 23
Cuts at: 1760 2453 2627 3018 3045 4390 179
Size: 693 174 391 27 1345 657
Fragments arranged by size:
1345 693 657 550 391 316 219 174
156 140 132 45 27 23
EagI C'GGCC_G
Cuts at: 179 2453 179
Size: 2274 2594
EarI CTCTTCn'nnn_
Cuts at: 1363 1390 2891 3101 1363
Size: 27 1501 210 3130
Fragments arranged by size:
3130 1501 210 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3076 3386 3532 4678 3076
Size: 310 146 1146 3266
Fragments arranged by size:
3266 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1244 1880 1244
Size: 636 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 727 771 1456 2693 3125 3872 4140 322
Size: 405 44 685 1237 432 747 268 1050
Fragments arranged by size:
1237 1050 747 685 432 405 268 44
EcoO109I rG'GnC_Cy
Cuts at: 195 540 663 1039 1257 1819 2007 195
Size: 345 123 376 218 562 188 3056
Fragments arranged by size:
3056 562 376 345 218 188 123
EcoRI G'AATT_C
Cuts at: 1872 1872
Size: 4868
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 4868
FspI TGC'GCA
Cuts at: 2649 2649
Size: 4868
HaeII r_GCGC'y
Cuts at: 583 634 915 1240 1552 1757 1790 2302 2550
Size: 51 281 325 312 205 33 512 248
Cuts at: 2550 3572 4551 583
Size: 1022 979 900
Fragments arranged by size:
1022 979 900 512 325 312 281 248
205 51 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2710 2740 2742 2772 2710
Size: 30 2 30 4806
Fragments arranged by size:
4806 30 30 2
HincII GTy'rAC
Cuts at: 1907 1907
Size: 4868
KasI G'GCGC_C
Cuts at: 579 630 1753 2298 2546 579
Size: 51 1123 545 248 2901
Fragments arranged by size:
2901 1123 545 248 51
KpnI G_GTAC'C
Cuts at: 769 1888 769
Size: 1119 3749
MluI A'CGCG_T
Cuts at: 4301 4301
Size: 4868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3539 3723 3539
Size: 184 4684
MscI TGG'CCA
Cuts at: 871 1090 2629 871
Size: 219 1539 3110
Fragments arranged by size:
3110 1539 219
MslI CAynn'nnrTG
Cuts at: 865 886 1498 2857 2984 865
Size: 21 612 1359 127 2749
Fragments arranged by size:
2749 1359 612 127 21
MspA1I CmG'CkG
Cuts at: 165 233 324 411 462 658 1329 1715 1731
Size: 68 91 87 51 196 671 386 16
Cuts at: 1731 1979 2293 2653 3666 3911 165
Size: 248 314 360 1013 245 1122
Fragments arranged by size:
1122 1013 671 386 360 314 248 245
196 91 87 68 51 16
NaeI GCC'GGC
Cuts at: 450 635 1140 1269 3049 450
Size: 185 505 129 1780 2269
Fragments arranged by size:
2269 1780 505 185 129
NarI GG'CG_CC
Cuts at: 580 631 1754 2299 2547 580
Size: 51 1123 545 248 2901
Fragments arranged by size:
2901 1123 545 248 51
NcoI C'CATG_G
Cuts at: 107 1757 107
Size: 1650 3218
NgoMIV G'CCGG_C
Cuts at: 448 633 1138 1267 3047 448
Size: 185 505 129 1780 2269
Fragments arranged by size:
2269 1780 505 185 129
NotI GC'GGCC_GC
Cuts at: 179 179
Size: 4868
NruI TCG'CGA
Cuts at: 686 686
Size: 4868
NspI r_CATG'y
Cuts at: 261 1921 2421 3328 261
Size: 1660 500 907 1801
Fragments arranged by size:
1801 1660 907 500
PciI A'CATG_T
Cuts at: 3324 3324
Size: 4868
PflMI CCAn_nnn'nTGG
Cuts at: 818 1103 818
Size: 285 4583
PmeI GTTT'AAAC
Cuts at: 1867 1867
Size: 4868
PmlI CAC'GTG
Cuts at: 1860 1860
Size: 4868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 790 822 1285 1317 4007 4039 790
Size: 32 463 32 2690 32 1619
Fragments arranged by size:
2690 1619 463 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 540 1257 540
Size: 717 4151
PspOMI G'GGCC_C
Cuts at: 663 1038 1323 1818 663
Size: 375 285 495 3713
Fragments arranged by size:
3713 495 375 285
PstI C_TGCA'G
Cuts at: 1003 1915 2600 1003
Size: 912 685 3271
Fragments arranged by size:
3271 912 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4868
PvuII CAG'CTG
Cuts at: 165 2293 2653 165
Size: 2128 360 2380
Fragments arranged by size:
2380 2128 360
RsrII CG'GwC_CG
Cuts at: 3063 3063
Size: 4868
SacI G_AGCT'C
Cuts at: 1246 1882 1246
Size: 636 4232
SacII CC_GC'GG
Cuts at: 234 1732 234
Size: 1498 3370
SalI G'TCGA_C
Cuts at: 1905 1905
Size: 4868
SanDI GG'GwC_CC
Cuts at: 1257 1257
Size: 4868
SapI GCTCTTCn'nnn_
Cuts at: 1363 1390 2891 3101 1363
Size: 27 1501 210 3130
Fragments arranged by size:
3130 1501 210 27
SbfI CC_TGCA'GG
Cuts at: 1915 1915
Size: 4868
ScaI AGT'ACT
Cuts at: 1183 1606 1627 4385 1183
Size: 423 21 2758 1666
Fragments arranged by size:
2758 1666 423 21
SexAI A'CCwGG_T
Cuts at: 1123 1123
Size: 4868
SfcI C'TryA_G
Cuts at: 33 999 1911 2596 3589 3780 33
Size: 966 912 685 993 191 1121
Fragments arranged by size:
1121 993 966 912 685 191
SfoI GGC'GCC
Cuts at: 581 632 1755 2300 2548 581
Size: 51 1123 545 248 2901
Fragments arranged by size:
2901 1123 545 248 51
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
SmaI CCC'GGG
Cuts at: 1568 1890 1568
Size: 322 4546
SmlI C'TyrA_G
Cuts at: 1621 2023 2938 3430 3692 1621
Size: 402 915 492 262 2797
Fragments arranged by size:
2797 915 492 402 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4868
SphI G_CATG'C
Cuts at: 1921 2421 1921
Size: 500 4368
SrfI GCCC'GGGC
Cuts at: 1568 1568
Size: 4868
StuI AGG'CCT
Cuts at: 591 827 591
Size: 236 4632
StyI C'CwwG_G
Cuts at: 107 191 443 1757 2002 2356 107
Size: 84 252 1314 245 354 2619
Fragments arranged by size:
2619 1314 354 252 245 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1599 2451 2584 3043 3252 1599
Size: 852 133 459 209 3215
Fragments arranged by size:
3215 852 459 209 133
TatI w'GTAC_w
Cuts at: 374 1181 1604 1625 4383 374
Size: 807 423 21 2758 859
Fragments arranged by size:
2758 859 807 423 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4385 4417 4385
Size: 32 4836
Tth111I GACn'n_nGTC
Cuts at: 2665 2665
Size: 4868
XbaI T'CTAG_A
Cuts at: 1899 1899
Size: 4868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1230 1497 2363 1230
Size: 267 866 3735
Fragments arranged by size:
3735 866 267
XmaI C'CCGG_G
Cuts at: 1566 1888 1566
Size: 322 4546
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NruI NspI
PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BsaXI BseRI BsiWI
BsmBI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NsiI PacI PasI PfoI PshAI PsiI PspXI PsrI
SfiI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI