Restriction map for
pF1KE2065
(Circular) (Six-base) MAPSORT of: pF1KE2065.seq Check: 5199 from: 1 to: 5270
pF1KE2065 5270 bp
With 182 enzymes: SgfI *
July 14, 2018 20:54 ..
AatII G_ACGT'C
Cuts at: 239 239
Size: 5270
AccI GT'mk_AC
Cuts at: 2308 2308
Size: 5270
Acc65I G'GTAC_C
Cuts at: 331 2286 331
Size: 1955 3315
AclI AA'CG_TT
Cuts at: 5110 5110
Size: 5270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 333 442 863 1336 1798 3095 3527 4274
Size: 134 109 421 473 462 1297 432 747
Cuts at: 4274 4542 199
Size: 268 927
Fragments arranged by size:
1297 927 747 473 462 432 421 268
134 109
AflII C'TTAA_G
Cuts at: 312 312
Size: 5270
AflIII A'CryG_T
Cuts at: 2622 3726 4703 2622
Size: 1104 977 3189
Fragments arranged by size:
3189 1104 977
AgeI A'CCGG_T
Cuts at: 3658 3658
Size: 5270
AlwNI CAG_nnn'CTG
Cuts at: 153 204 578 2575 4142 153
Size: 51 374 1997 1567 1281
Fragments arranged by size:
1997 1567 1281 374 51
ApaLI G'TGCA_C
Cuts at: 4040 4040
Size: 5270
ApoI r'AATT_y
Cuts at: 2274 4623 4879 2274
Size: 2349 256 2665
Fragments arranged by size:
2665 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5270
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5270
AvaI C'yCGr_G
Cuts at: 1606 1653 2290 1606
Size: 47 637 4586
Fragments arranged by size:
4586 637 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 323 356 323
Size: 33 5237
BamHI G'GATC_C
Cuts at: 984 2189 2295 984
Size: 1205 106 3959
Fragments arranged by size:
3959 1205 106
BanI G'GyrC_C
Cuts at: 138 331 2286 2700 2948 2983 138
Size: 193 1955 414 248 35 2425
Fragments arranged by size:
2425 1955 414 248 193 35
BanII G_rGCy'C
Cuts at: 596 1625 2284 3314 596
Size: 1029 659 1030 2552
Fragments arranged by size:
2552 1030 1029 659
BbeI G_GCGC'C
Cuts at: 2704 2952 2704
Size: 248 5022
BbsI GAAGACnn'nnnn_
Cuts at: 1324 1324
Size: 5270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1597 1631 1597
Size: 34 5236
BciVI GTATCCnnnnn_n'
Cuts at: 3168 3929 5270 3168
Size: 761 1341 3168
Fragments arranged by size:
3168 1341 761
BclI T'GATC_A
Cuts at: 869 2790 869
Size: 1921 3349
BglII A'GATC_T
Cuts at: 2785 4822 2785
Size: 2037 3233
BlpI GC'TnA_GC
Cuts at: 1501 2382 1501
Size: 881 4389
Bme1580I G_kGCm'C
Cuts at: 2895 2988 4044 2895
Size: 93 1056 4121
Fragments arranged by size:
4121 1056 93
BmgBI CAC'GTC
Cuts at: 2040 2040
Size: 5270
BmrI ACTGGGnnnn_n'
Cuts at: 419 1582 2482 2563 2898 5042 419
Size: 1163 900 81 335 2144 647
Fragments arranged by size:
2144 1163 900 647 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1526 2232 3637 1526
Size: 706 1405 3159
Fragments arranged by size:
3159 1405 706
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 802 883 1159 1915 643
Size: 159 81 276 756 3998
Fragments arranged by size:
3998 756 276 159 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 3325 3817 4115 2446
Size: 879 492 298 3601
Fragments arranged by size:
3601 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 922 1954 4974 922
Size: 1032 3020 1218
Fragments arranged by size:
3020 1218 1032
BsaAI yAC'GTr
Cuts at: 2623 2623
Size: 5270
BsaBI GATnn'nnATC
Cuts at: 6 1334 2784 4821 6
Size: 1328 1450 2037 455
Fragments arranged by size:
2037 1450 1328 455
BsaHI Gr'CG_yC
Cuts at: 236 848 2701 2949 3651 5147 236
Size: 612 1853 248 702 1496 359
Fragments arranged by size:
1853 1496 702 612 359 248
BsaWI w'CCGG_w
Cuts at: 1883 2459 2980 3658 3932 4079 4490 1883
Size: 576 521 678 274 147 411 2663
Fragments arranged by size:
2663 678 576 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 874 904 874
Size: 30 5240
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1462 1465 1673 1462
Size: 3 208 5059
Fragments arranged by size:
5059 208 3
BseYI C'CCAG_C
Cuts at: 125 153 571 1600 2019 2695 3453 4030 4526
Size: 28 418 1029 419 676 758 577 496
Cuts at: 4526 125
Size: 869
Fragments arranged by size:
1029 869 758 676 577 496 419 418
28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 837 934 1079 1103 289
Size: 548 97 145 24 4456
Fragments arranged by size:
4456 548 145 97 24
BsiEI CG_ry'CG
Cuts at: 104 2812 2858 4066 104
Size: 2708 46 1208 1308
Fragments arranged by size:
2708 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 596 2284 3062 3252 4044 596
Size: 1688 778 190 792 1822
Fragments arranged by size:
1822 1688 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 596 1625 2284 2895 2988 3062 3252 3314 4044
Size: 1029 659 611 93 74 190 62 730
Cuts at: 4044 596
Size: 1822
Fragments arranged by size:
1822 1029 730 659 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1883 4490 1883
Size: 2607 2663
BspHI T'CATG_A
Cuts at: 4446 4446
Size: 5270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1101 2320 2534 2836 3217 1101
Size: 1219 214 302 381 3154
Fragments arranged by size:
3154 1219 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1389 3562 3616 5100 5265 50
Size: 1339 2173 54 1484 165 55
Fragments arranged by size:
2173 1484 1339 165 55 54
BsrDI GCAATG_nn'
Cuts at: 836 1039 3182 836
Size: 203 2143 2924
Fragments arranged by size:
2924 2143 203
BsrFI r'CCGG_y
Cuts at: 3268 3449 3658 3268
Size: 181 209 4880
Fragments arranged by size:
4880 209 181
BssSI C'ACGA_G
Cuts at: 3541 3899 3541
Size: 358 4912
BstAPI GCAn_nnn'nTGC
Cuts at: 2647 2647
Size: 5270
BstBI TT'CG_AA
Cuts at: 3631 3631
Size: 5270
BstYI r'GATC_y
Cuts at: 1 464 581 601 984 1271 2189 2295 2762
Size: 463 117 20 383 287 918 106 467
Cuts at: 2762 2785 3120 3366 4378 4464 4476 4822 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 918 467 463 449 383 346 335
287 246 117 106 86 23 20 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3220 3401 3564 3220
Size: 181 163 4926
Fragments arranged by size:
4926 181 163
BtsI GCAGTG_nn'
Cuts at: 1868 2513 1868
Size: 645 4625
DraI TTT'AAA
Cuts at: 2269 2269
Size: 5270
DraIII CAC_nnn'GTG
Cuts at: 443 443
Size: 5270
DrdI GACnn_nn'nnGTC
Cuts at: 2976 3834 2976
Size: 858 4412
EaeI y'GGCC_r
Cuts at: 278 536 2855 3029 3420 3447 4792 278
Size: 258 2319 174 391 27 1345 756
Fragments arranged by size:
2319 1345 756 391 258 174 27
EagI C'GGCC_G
Cuts at: 2855 2855
Size: 5270
EarI CTCTTCn'nnn_
Cuts at: 349 373 1132 3293 3503 349
Size: 24 759 2161 210 2116
Fragments arranged by size:
2161 2116 759 210 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3478 3788 3934 5080 3478
Size: 310 146 1146 3668
Fragments arranged by size:
3668 1146 310 146
EcoICRI GAG'CTC
Cuts at: 594 2282 594
Size: 1688 3582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 333 442 643 802 863 883 1159 1336
Size: 134 109 201 159 61 20 276 177
Cuts at: 1336 1798 1915 3095 3527 4274 4542 199
Size: 462 117 1180 432 747 268 927
Fragments arranged by size:
1180 927 747 462 432 276 268 201
177 159 134 117 109 61 20
EcoNI CCTnn'n_nnAGG
Cuts at: 268 1434 268
Size: 1166 4104
EcoO109I rG'GnC_Cy
Cuts at: 2409 2409
Size: 5270
EcoRI G'AATT_C
Cuts at: 2274 2274
Size: 5270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1827 3289 1827
Size: 1462 3808
FspI TGC'GCA
Cuts at: 3051 3051
Size: 5270
HaeII r_GCGC'y
Cuts at: 2704 2952 3974 4953 2704
Size: 248 1022 979 3021
Fragments arranged by size:
3021 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 745 777 2174 2206 3112 3142 3144 3174 745
Size: 32 1397 32 906 30 2 30 2841
Fragments arranged by size:
2841 1397 906 32 32 30 30 2
HincII GTy'rAC
Cuts at: 909 993 1064 1218 2309 909
Size: 84 71 154 1091 3870
Fragments arranged by size:
3870 1091 154 84 71
HpaI GTT'AAC
Cuts at: 1218 1218
Size: 5270
KasI G'GCGC_C
Cuts at: 2700 2948 2700
Size: 248 5022
KpnI G_GTAC'C
Cuts at: 335 2290 335
Size: 1955 3315
MluI A'CGCG_T
Cuts at: 4703 4703
Size: 5270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1278 3941 4125 1278
Size: 2663 184 2423
Fragments arranged by size:
2663 2423 184
MscI TGG'CCA
Cuts at: 280 3031 280
Size: 2751 2519
MslI CAynn'nnrTG
Cuts at: 496 3259 3386 496
Size: 2763 127 2380
Fragments arranged by size:
2763 2380 127
MspA1I CmG'CkG
Cuts at: 129 1422 1581 2381 2695 3055 4068 4313 129
Size: 1293 159 800 314 360 1013 245 1086
Fragments arranged by size:
1293 1086 1013 800 360 314 245 159
NaeI GCC'GGC
Cuts at: 3451 3451
Size: 5270
NarI GG'CG_CC
Cuts at: 2701 2949 2701
Size: 248 5022
NcoI C'CATG_G
Cuts at: 107 107
Size: 5270
NgoMIV G'CCGG_C
Cuts at: 3449 3449
Size: 5270
NspI r_CATG'y
Cuts at: 2323 2823 3730 2323
Size: 500 907 3863
Fragments arranged by size:
3863 907 500
PasI CC'CwG_GG
Cuts at: 1493 1493
Size: 5270
PciI A'CATG_T
Cuts at: 3726 3726
Size: 5270
PflMI CCAn_nnn'nTGG
Cuts at: 578 1149 578
Size: 571 4699
PfoI T'CCnGG_A
Cuts at: 167 368 684 1892 167
Size: 201 316 1208 3545
Fragments arranged by size:
3545 1208 316 201
PmeI GTTT'AAAC
Cuts at: 2269 2269
Size: 5270
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4409 4441 4409
Size: 32 5238
PshAI GACnn'nnGTC
Cuts at: 680 680
Size: 5270
PstI C_TGCA'G
Cuts at: 245 1691 1875 2317 3002 245
Size: 1446 184 442 685 2513
Fragments arranged by size:
2513 1446 685 442 184
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5270
PvuII CAG'CTG
Cuts at: 129 1422 1581 2695 3055 129
Size: 1293 159 1114 360 2344
Fragments arranged by size:
2344 1293 1114 360 159
RsrII CG'GwC_CG
Cuts at: 3465 3465
Size: 5270
SacI G_AGCT'C
Cuts at: 596 2284 596
Size: 1688 3582
SalI G'TCGA_C
Cuts at: 2307 2307
Size: 5270
SapI GCTCTTCn'nnn_
Cuts at: 3293 3503 3293
Size: 210 5060
SbfI CC_TGCA'GG
Cuts at: 245 2317 245
Size: 2072 3198
ScaI AGT'ACT
Cuts at: 4787 4787
Size: 5270
SexAI A'CCwGG_T
Cuts at: 1057 1057
Size: 5270
SfcI C'TryA_G
Cuts at: 33 241 1687 1871 2313 2998 3991 4182 33
Size: 208 1446 184 442 685 993 191 1121
Fragments arranged by size:
1446 1121 993 685 442 208 191 184
SfoI GGC'GCC
Cuts at: 2702 2950 2702
Size: 248 5022
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5270
SmaI CCC'GGG
Cuts at: 1655 2292 1655
Size: 637 4633
SmlI C'TyrA_G
Cuts at: 312 2425 3340 3832 4094 312
Size: 2113 915 492 262 1488
Fragments arranged by size:
2113 1488 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5270
SphI G_CATG'C
Cuts at: 2323 2823 2323
Size: 500 4770
StuI AGG'CCT
Cuts at: 2004 2004
Size: 5270
StyI C'CwwG_G
Cuts at: 107 605 1811 2143 2404 2758 107
Size: 498 1206 332 261 354 2619
Fragments arranged by size:
2619 1206 498 354 332 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1614 2032 2853 2986 3445 3654 1614
Size: 418 821 133 459 209 3230
Fragments arranged by size:
3230 821 459 418 209 133
TatI w'GTAC_w
Cuts at: 4785 4785
Size: 5270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 799 831 874 906 2044 2076 4787 4819 799
Size: 32 43 32 1138 32 2711 32 1250
Fragments arranged by size:
2711 1250 1138 43 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3067 3067
Size: 5270
XbaI T'CTAG_A
Cuts at: 2301 2301
Size: 5270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2037 2141 2765 2037
Size: 104 624 4542
Fragments arranged by size:
4542 624 104
XmaI C'CCGG_G
Cuts at: 1653 2290 1653
Size: 637 4633
ZraI GAC'GTC
Cuts at: 237 237
Size: 5270
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BbvCI BfrBI BglI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI