Restriction map for
pF1KE2025
(Circular) (Six-base) MAPSORT of: pF1KE2025.seq Check: 3723 from: 1 to: 4937
pF1KE2025 4937 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 846 846
Size: 4937
AatII G_ACGT'C
Cuts at: 983 983
Size: 4937
AccI GT'mk_AC
Cuts at: 1975 1975
Size: 4937
Acc65I G'GTAC_C
Cuts at: 1046 1352 1953 1046
Size: 306 601 4030
Fragments arranged by size:
4030 601 306
AclI AA'CG_TT
Cuts at: 4777 4777
Size: 4937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 2762 3194 3941 4209 964
Size: 1798 432 747 268 1692
Fragments arranged by size:
1798 1692 747 432 268
AflIII A'CryG_T
Cuts at: 959 1417 1592 2289 3393 4370 959
Size: 458 175 697 1104 977 1526
Fragments arranged by size:
1526 1104 977 697 458 175
AgeI A'CCGG_T
Cuts at: 3325 3325
Size: 4937
AlwNI CAG_nnn'CTG
Cuts at: 273 2242 3809 273
Size: 1969 1567 1401
Fragments arranged by size:
1969 1567 1401
ApaI G_GGCC'C
Cuts at: 182 182
Size: 4937
ApaLI G'TGCA_C
Cuts at: 3707 3707
Size: 4937
ApoI r'AATT_y
Cuts at: 1231 1941 4290 4546 1231
Size: 710 2349 256 1622
Fragments arranged by size:
2349 1622 710 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4937
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4937
AvaI C'yCGr_G
Cuts at: 165 280 362 822 850 1957 165
Size: 115 82 460 28 1107 3145
Fragments arranged by size:
3145 1107 460 115 82 28
BamHI G'GATC_C
Cuts at: 695 1145 1962 695
Size: 450 817 3670
Fragments arranged by size:
3670 817 450
BanI G'GyrC_C
Cuts at: 208 616 1046 1090 1352 1364 1801 1953 2367
Size: 408 430 44 262 12 437 152 414
Cuts at: 2367 2615 2650 208
Size: 248 35 2495
Fragments arranged by size:
2495 437 430 414 408 262 248 152
44 35 12
BanII G_rGCy'C
Cuts at: 182 881 1340 1765 1951 2981 182
Size: 699 459 425 186 1030 2138
Fragments arranged by size:
2138 1030 699 459 425 186
BbeI G_GCGC'C
Cuts at: 212 1805 2371 2619 212
Size: 1593 566 248 2530
Fragments arranged by size:
2530 1593 566 248
BbsI GAAGACnn'nnnn_
Cuts at: 530 530
Size: 4937
BbvCI CC'TCA_GC
Cuts at: 773 1621 773
Size: 848 4089
BciVI GTATCCnnnnn_n'
Cuts at: 2835 3596 4937 2835
Size: 761 1341 2835
Fragments arranged by size:
2835 1341 761
BclI T'GATC_A
Cuts at: 2457 2457
Size: 4937
BglI GCCn_nnn'nGGC
Cuts at: 386 386
Size: 4937
BglII A'GATC_T
Cuts at: 2452 4489 2452
Size: 2037 2900
BlpI GC'TnA_GC
Cuts at: 2049 2049
Size: 4937
Bme1580I G_kGCm'C
Cuts at: 182 1543 1836 2562 2655 3711 182
Size: 1361 293 726 93 1056 1408
Fragments arranged by size:
1408 1361 1056 726 293 93
BmrI ACTGGGnnnn_n'
Cuts at: 2149 2230 2565 4709 2149
Size: 81 335 2144 2377
Fragments arranged by size:
2377 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3304 3304
Size: 4937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 743 934 1674 523
Size: 220 191 740 3786
Fragments arranged by size:
3786 740 220 191
Bpu10I CC'TnA_GC
Cuts at: 773 1621 773
Size: 848 4089
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1686 2113 2992 3484 3782 1686
Size: 427 879 492 298 2841
Fragments arranged by size:
2841 879 492 427 298
BsaI GGTCTCn'nnnn_
Cuts at: 4641 4641
Size: 4937
BsaAI yAC'GTr
Cuts at: 606 2290 606
Size: 1684 3253
BsaBI GATnn'nnATC
Cuts at: 6 1219 2451 4488 6
Size: 1213 1232 2037 455
Fragments arranged by size:
2037 1232 1213 455
BsaHI Gr'CG_yC
Cuts at: 209 980 1802 1888 2368 2616 3318 4814 209
Size: 771 822 86 480 248 702 1496 332
Fragments arranged by size:
1496 822 771 702 480 332 248 86
BsaWI w'CCGG_w
Cuts at: 1142 2126 2647 3325 3599 3746 4157 1142
Size: 984 521 678 274 147 411 1922
Fragments arranged by size:
1922 984 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 826 856 1735 1765 826
Size: 30 879 30 3998
Fragments arranged by size:
3998 879 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 547 850 208
Size: 339 303 4295
Fragments arranged by size:
4295 339 303
BseYI C'CCAG_C
Cuts at: 578 764 949 2362 3120 3697 4193 578
Size: 186 185 1413 758 577 496 1322
Fragments arranged by size:
1413 1322 758 577 496 186 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 568 396
Size: 172 4765
BsiEI CG_ry'CG
Cuts at: 104 1655 2479 2525 3733 104
Size: 1551 824 46 1208 1308
Fragments arranged by size:
1551 1308 1208 824 46
BsiHKAI G_wGCw'C
Cuts at: 355 729 1777 1951 2729 2919 3711 355
Size: 374 1048 174 778 190 792 1581
Fragments arranged by size:
1581 1048 792 778 374 190 174
BsmI GAATG_Cn'
Cuts at: 1245 1245
Size: 4937
Bsp1286I G_dGCh'C
Cuts at: 182 355 729 881 1340 1543 1765 1777 1836
Size: 173 374 152 459 203 222 12 59
Cuts at: 1836 1951 2562 2655 2729 2919 2981 3711 182
Size: 115 611 93 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 459 374 222 203 190
173 152 115 93 74 62 59 12
BspEI T'CCGG_A
Cuts at: 1142 4157 1142
Size: 3015 1922
BspHI T'CATG_A
Cuts at: 4113 4113
Size: 4937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 493 846 1987 2201 2503 2884 493
Size: 353 1141 214 302 381 2546
Fragments arranged by size:
2546 1141 381 353 302 214
BsrBI CCG'CTC
Cuts at: 50 507 525 3229 3283 4767 4932 50
Size: 457 18 2704 54 1484 165 55
Fragments arranged by size:
2704 1484 457 165 55 54 18
BsrDI GCAATG_nn'
Cuts at: 2849 2849
Size: 4937
BsrFI r'CCGG_y
Cuts at: 292 1483 2935 3116 3325 292
Size: 1191 1452 181 209 1904
Fragments arranged by size:
1904 1452 1191 209 181
BssHII G'CGCG_C
Cuts at: 288 627 288
Size: 339 4598
BssSI C'ACGA_G
Cuts at: 1827 3208 3566 1827
Size: 1381 358 3198
Fragments arranged by size:
3198 1381 358
BstAPI GCAn_nnn'nTGC
Cuts at: 273 2314 273
Size: 2041 2896
BstBI TT'CG_AA
Cuts at: 3298 3298
Size: 4937
BstXI CCAn_nnnn'nTGG
Cuts at: 565 759 1813 1884 565
Size: 194 1054 71 3618
Fragments arranged by size:
3618 1054 194 71
BstYI r'GATC_y
Cuts at: 1 245 695 1145 1509 1962 2429 2452 2787
Size: 244 450 450 364 453 467 23 335
Cuts at: 2787 3033 4045 4131 4143 4489 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 453 450 450 449 364 346
335 246 244 86 23 12
BtgI C'CryG_G
Cuts at: 107 758 881 1062 1094 1260 1637 1862 1883
Size: 651 123 181 32 166 377 225 21
Cuts at: 1883 107
Size: 3161
Fragments arranged by size:
3161 651 377 225 181 166 123 32
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 297 2887 3068 3231 297
Size: 2590 181 163 2003
Fragments arranged by size:
2590 2003 181 163
BtsI GCAGTG_nn'
Cuts at: 1677 1818 2180 1677
Size: 141 362 4434
Fragments arranged by size:
4434 362 141
DraI TTT'AAA
Cuts at: 1936 1936
Size: 4937
DrdI GACnn_nn'nnGTC
Cuts at: 534 2643 3501 534
Size: 2109 858 1970
Fragments arranged by size:
2109 1970 858
EaeI y'GGCC_r
Cuts at: 1154 1634 2522 2696 3087 3114 4459 1154
Size: 480 888 174 391 27 1345 1632
Fragments arranged by size:
1632 1345 888 480 391 174 27
EagI C'GGCC_G
Cuts at: 2522 2522
Size: 4937
EarI CTCTTCn'nnn_
Cuts at: 781 901 2960 3170 781
Size: 120 2059 210 2548
Fragments arranged by size:
2548 2059 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3145 3455 3601 4747 3145
Size: 310 146 1146 3335
Fragments arranged by size:
3335 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1949 1949
Size: 4937
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 743 934 964 1674 2762 3194 3941 4209
Size: 220 191 30 710 1088 432 747 268
Cuts at: 4209 523
Size: 1251
Fragments arranged by size:
1251 1088 747 710 432 268 220 191
30
EcoO109I rG'GnC_Cy
Cuts at: 178 179 1793 2076 178
Size: 1 1614 283 3039
Fragments arranged by size:
3039 1614 283 1
EcoRI G'AATT_C
Cuts at: 1941 1941
Size: 4937
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 414 1056 2956 414
Size: 642 1900 2395
Fragments arranged by size:
2395 1900 642
FspI TGC'GCA
Cuts at: 2718 2718
Size: 4937
HaeII r_GCGC'y
Cuts at: 212 274 1805 2371 2619 3641 4620 212
Size: 62 1531 566 248 1022 979 529
Fragments arranged by size:
1531 1022 979 566 529 248 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 826 858 1393 1425 1725 1757 2779 2809 2811
Size: 32 535 32 300 32 1022 30 2
Cuts at: 2811 2841 826
Size: 30 2922
Fragments arranged by size:
2922 1022 535 300 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1848 1976 1848
Size: 128 4809
KasI G'GCGC_C
Cuts at: 208 1801 2367 2615 208
Size: 1593 566 248 2530
Fragments arranged by size:
2530 1593 566 248
KpnI G_GTAC'C
Cuts at: 1050 1356 1957 1050
Size: 306 601 4030
Fragments arranged by size:
4030 601 306
MluI A'CGCG_T
Cuts at: 4370 4370
Size: 4937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3608 3792 3608
Size: 184 4753
MscI TGG'CCA
Cuts at: 1156 2698 1156
Size: 1542 3395
MslI CAynn'nnrTG
Cuts at: 332 1882 2926 3053 332
Size: 1550 1044 127 2216
Fragments arranged by size:
2216 1550 1044 127
MspA1I CmG'CkG
Cuts at: 115 252 953 1096 1628 2048 2362 2722 3735
Size: 137 701 143 532 420 314 360 1013
Cuts at: 3735 3980 115
Size: 245 1072
Fragments arranged by size:
1072 1013 701 532 420 360 314 245
143 137
NaeI GCC'GGC
Cuts at: 294 3118 294
Size: 2824 2113
NarI GG'CG_CC
Cuts at: 209 1802 2368 2616 209
Size: 1593 566 248 2530
Fragments arranged by size:
2530 1593 566 248
NcoI C'CATG_G
Cuts at: 107 758 881 1062 1862 1883 107
Size: 651 123 181 800 21 3161
Fragments arranged by size:
3161 800 651 181 123 21
NgoMIV G'CCGG_C
Cuts at: 292 3116 292
Size: 2824 2113
NspI r_CATG'y
Cuts at: 894 912 963 1242 1421 1596 1990 2490 3397
Size: 18 51 279 179 175 394 500 907
Cuts at: 3397 894
Size: 2434
Fragments arranged by size:
2434 907 500 394 279 179 175 51
18
PasI CC'CwG_GG
Cuts at: 411 1806 411
Size: 1395 3542
PciI A'CATG_T
Cuts at: 959 1417 1592 3393 959
Size: 458 175 1801 2503
Fragments arranged by size:
2503 1801 458 175
PflMI CCAn_nnn'nTGG
Cuts at: 501 1043 1068 1266 501
Size: 542 25 198 4172
Fragments arranged by size:
4172 542 198 25
PfoI T'CCnGG_A
Cuts at: 1505 1753 1505
Size: 248 4689
PmeI GTTT'AAAC
Cuts at: 1936 1936
Size: 4937
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 4076
Size: 32 4905
PpuMI rG'GwC_Cy
Cuts at: 1793 1793
Size: 4937
PspOMI G'GGCC_C
Cuts at: 178 178
Size: 4937
PspXI vC'TCGA_Gb
Cuts at: 280 280
Size: 4937
PstI C_TGCA'G
Cuts at: 1984 2669 1984
Size: 685 4252
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4937
PvuII CAG'CTG
Cuts at: 2362 2722 2362
Size: 360 4577
RsrII CG'GwC_CG
Cuts at: 804 3132 804
Size: 2328 2609
SacI G_AGCT'C
Cuts at: 1951 1951
Size: 4937
SacII CC_GC'GG
Cuts at: 1097 1097
Size: 4937
SalI G'TCGA_C
Cuts at: 1974 1974
Size: 4937
SapI GCTCTTCn'nnn_
Cuts at: 901 2960 3170 901
Size: 2059 210 2668
Fragments arranged by size:
2668 2059 210
SbfI CC_TGCA'GG
Cuts at: 1984 1984
Size: 4937
ScaI AGT'ACT
Cuts at: 553 4454 553
Size: 3901 1036
SexAI A'CCwGG_T
Cuts at: 1355 1355
Size: 4937
SfcI C'TryA_G
Cuts at: 33 321 1980 2665 3658 3849 33
Size: 288 1659 685 993 191 1121
Fragments arranged by size:
1659 1121 993 685 288 191
SfoI GGC'GCC
Cuts at: 210 1803 2369 2617 210
Size: 1593 566 248 2530
Fragments arranged by size:
2530 1593 566 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4937
SmaI CCC'GGG
Cuts at: 167 824 1959 167
Size: 657 1135 3145
Fragments arranged by size:
3145 1135 657
SmlI C'TyrA_G
Cuts at: 280 1665 2092 3007 3499 3761 280
Size: 1385 427 915 492 262 1456
Fragments arranged by size:
1456 1385 915 492 427 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4937
SphI G_CATG'C
Cuts at: 912 1990 2490 912
Size: 1078 500 3359
Fragments arranged by size:
3359 1078 500
StyI C'CwwG_G
Cuts at: 107 758 881 1031 1062 1391 1788 1862 1883
Size: 651 123 150 31 329 397 74 21
Cuts at: 1883 2071 2425 107
Size: 188 354 2619
Fragments arranged by size:
2619 651 397 354 329 188 150 123
74 31 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 462 1762 2520 2653 3112 3321 462
Size: 1300 758 133 459 209 2078
Fragments arranged by size:
2078 1300 758 459 209 133
TatI w'GTAC_w
Cuts at: 551 1169 4452 551
Size: 618 3283 1036
Fragments arranged by size:
3283 1036 618
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1579 1611 1735 1767 4454 4486 1579
Size: 32 124 32 2687 32 2030
Fragments arranged by size:
2687 2030 124 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2734 2734
Size: 4937
XbaI T'CTAG_A
Cuts at: 1968 1968
Size: 4937
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1503 1813 2432 1503
Size: 310 619 4008
Fragments arranged by size:
4008 619 310
XhoI C'TCGA_G
Cuts at: 280 280
Size: 4937
XmaI C'CCGG_G
Cuts at: 165 822 1957 165
Size: 657 1135 3145
Fragments arranged by size:
3145 1135 657
ZraI GAC'GTC
Cuts at: 981 981
Size: 4937
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PsrI SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI