Restriction map for
pF1KE2008
(Circular) (Six-base) MAPSORT of: pF1KE2008.seq Check: 3113 from: 1 to: 4862
pF1KE2008 4862 bp
With 182 enzymes: SgfI *
April 23, 2019 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 772 840 772
Size: 68 4794
AccI GT'mk_AC
Cuts at: 193 1745 1900 193
Size: 1552 155 3155
Fragments arranged by size:
3155 1552 155
Acc65I G'GTAC_C
Cuts at: 1878 1878
Size: 4862
AclI AA'CG_TT
Cuts at: 4702 4702
Size: 4862
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 300 1559 1871 2687 3119 3866 4134 156
Size: 144 1259 312 816 432 747 268 884
Fragments arranged by size:
1259 884 816 747 432 312 268 144
AflIII A'CryG_T
Cuts at: 467 1785 2214 3318 4295 467
Size: 1318 429 1104 977 1034
Fragments arranged by size:
1318 1104 1034 977 429
AgeI A'CCGG_T
Cuts at: 3250 3250
Size: 4862
AlwNI CAG_nnn'CTG
Cuts at: 131 2167 3734 131
Size: 2036 1567 1259
Fragments arranged by size:
2036 1567 1259
ApaLI G'TGCA_C
Cuts at: 3632 3632
Size: 4862
ApoI r'AATT_y
Cuts at: 201 946 1866 4215 4471 201
Size: 745 920 2349 256 592
Fragments arranged by size:
2349 920 745 592 256
AseI AT'TA_AT
Cuts at: 20 1502 20
Size: 1482 3380
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4862
AvaI C'yCGr_G
Cuts at: 1882 1882
Size: 4862
BamHI G'GATC_C
Cuts at: 1887 1887
Size: 4862
BanI G'GyrC_C
Cuts at: 477 1878 2292 2540 2575 477
Size: 1401 414 248 35 2764
Fragments arranged by size:
2764 1401 414 248 35
BanII G_rGCy'C
Cuts at: 1876 2906 1876
Size: 1030 3832
BbeI G_GCGC'C
Cuts at: 2296 2544 2296
Size: 248 4614
BbsI GAAGACnn'nnnn_
Cuts at: 162 593 1243 162
Size: 431 650 3781
Fragments arranged by size:
3781 650 431
BciVI GTATCCnnnnn_n'
Cuts at: 2760 3521 4862 2760
Size: 761 1341 2760
Fragments arranged by size:
2760 1341 761
BclI T'GATC_A
Cuts at: 2382 2382
Size: 4862
BglII A'GATC_T
Cuts at: 2377 4414 2377
Size: 2037 2825
BlpI GC'TnA_GC
Cuts at: 1974 1974
Size: 4862
Bme1580I G_kGCm'C
Cuts at: 2487 2580 3636 2487
Size: 93 1056 3713
Fragments arranged by size:
3713 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 295 682 1379 2074 2155 2490 4634 295
Size: 387 697 695 81 335 2144 523
Fragments arranged by size:
2144 697 695 523 387 335 81
BmtI G_CTAG'C
Cuts at: 1184 1184
Size: 4862
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3229 3229
Size: 4862
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1600 742
Size: 858 4004
Bpu10I CC'TnA_GC
Cuts at: 918 918
Size: 4862
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2038 2917 3409 3707 2038
Size: 879 492 298 3193
Fragments arranged by size:
3193 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1717 4566 1717
Size: 2849 2013
BsaAI yAC'GTr
Cuts at: 2215 2215
Size: 4862
BsaBI GATnn'nnATC
Cuts at: 6 2376 4413 6
Size: 2370 2037 455
Fragments arranged by size:
2370 2037 455
BsaHI Gr'CG_yC
Cuts at: 2293 2541 3243 4739 2293
Size: 248 702 1496 2416
Fragments arranged by size:
2416 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2051 2572 3250 3524 3671 4082 2051
Size: 521 678 274 147 411 2831
Fragments arranged by size:
2831 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 644 674 644
Size: 30 4832
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1705 1705
Size: 4862
BseYI C'CCAG_C
Cuts at: 2287 3045 3622 4118 2287
Size: 758 577 496 3031
Fragments arranged by size:
3031 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 4862
BsiEI CG_ry'CG
Cuts at: 104 2404 2450 3658 104
Size: 2300 46 1208 1308
Fragments arranged by size:
2300 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1876 2654 2844 3636 1876
Size: 778 190 792 3102
Fragments arranged by size:
3102 792 778 190
BsmI GAATG_Cn'
Cuts at: 1194 1194
Size: 4862
Bsp1286I G_dGCh'C
Cuts at: 1876 2487 2580 2654 2844 2906 3636 1876
Size: 611 93 74 190 62 730 3102
Fragments arranged by size:
3102 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4082 4082
Size: 4862
BspHI T'CATG_A
Cuts at: 4038 4038
Size: 4862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 772 840 1499 1912 2126 2428 2809 772
Size: 68 659 413 214 302 381 2825
Fragments arranged by size:
2825 659 413 381 302 214 68
BsrBI CCG'CTC
Cuts at: 50 655 3154 3208 4692 4857 50
Size: 605 2499 54 1484 165 55
Fragments arranged by size:
2499 1484 605 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1029 2774 1029
Size: 1745 3117
BsrFI r'CCGG_y
Cuts at: 2860 3041 3250 2860
Size: 181 209 4472
Fragments arranged by size:
4472 209 181
BsrGI T'GTAC_A
Cuts at: 1754 1754
Size: 4862
BssSI C'ACGA_G
Cuts at: 3133 3491 3133
Size: 358 4504
BstAPI GCAn_nnn'nTGC
Cuts at: 2239 2239
Size: 4862
BstBI TT'CG_AA
Cuts at: 3223 3223
Size: 4862
BstYI r'GATC_y
Cuts at: 1 319 1887 2354 2377 2712 2958 3970 4056
Size: 318 1568 467 23 335 246 1012 86
Cuts at: 4056 4068 4414 1
Size: 12 346 449
Fragments arranged by size:
1568 1012 467 449 346 335 318 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 194 194
Size: 4862
BtgI C'CryG_G
Cuts at: 107 107
Size: 4862
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2812 2993 3156 2812
Size: 181 163 4518
Fragments arranged by size:
4518 181 163
BtsI GCAGTG_nn'
Cuts at: 1467 2105 1467
Size: 638 4224
DraI TTT'AAA
Cuts at: 672 1861 672
Size: 1189 3673
DrdI GACnn_nn'nnGTC
Cuts at: 2568 3426 2568
Size: 858 4004
EaeI y'GGCC_r
Cuts at: 792 2447 2621 3012 3039 4384 792
Size: 1655 174 391 27 1345 1270
Fragments arranged by size:
1655 1345 1270 391 174 27
EagI C'GGCC_G
Cuts at: 2447 2447
Size: 4862
EarI CTCTTCn'nnn_
Cuts at: 148 1535 2885 3095 148
Size: 1387 1350 210 1915
Fragments arranged by size:
1915 1387 1350 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 150 3070 3380 3526 4672 150
Size: 2920 310 146 1146 340
Fragments arranged by size:
2920 1146 340 310 146
EcoICRI GAG'CTC
Cuts at: 1874 1874
Size: 4862
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 300 742 1559 1600 1871 2687 3119 3866
Size: 144 442 817 41 271 816 432 747
Cuts at: 3866 4134 156
Size: 268 884
Fragments arranged by size:
884 817 816 747 442 432 271 268
144 41
EcoO109I rG'GnC_Cy
Cuts at: 1797 2001 1797
Size: 204 4658
EcoRI G'AATT_C
Cuts at: 1866 1866
Size: 4862
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 4862
FspI TGC'GCA
Cuts at: 2643 2643
Size: 4862
HaeII r_GCGC'y
Cuts at: 2296 2544 3566 4545 2296
Size: 248 1022 979 2613
Fragments arranged by size:
2613 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 365 397 644 676 2704 2734 2736 2766 365
Size: 32 247 32 2028 30 2 30 2461
Fragments arranged by size:
2461 2028 247 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1901 1901
Size: 4862
KasI G'GCGC_C
Cuts at: 2292 2540 2292
Size: 248 4614
KpnI G_GTAC'C
Cuts at: 1882 1882
Size: 4862
MluI A'CGCG_T
Cuts at: 4295 4295
Size: 4862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3533 3717 3533
Size: 184 4678
MscI TGG'CCA
Cuts at: 794 2623 794
Size: 1829 3033
MslI CAynn'nnrTG
Cuts at: 751 2851 2978 751
Size: 2100 127 2635
Fragments arranged by size:
2635 2100 127
MspA1I CmG'CkG
Cuts at: 1973 2287 2647 3660 3905 1973
Size: 314 360 1013 245 2930
Fragments arranged by size:
2930 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3043 3043
Size: 4862
NarI GG'CG_CC
Cuts at: 2293 2541 2293
Size: 248 4614
NcoI C'CATG_G
Cuts at: 107 107
Size: 4862
NdeI CA'TA_TG
Cuts at: 210 210
Size: 4862
NgoMIV G'CCGG_C
Cuts at: 3041 3041
Size: 4862
NheI G'CTAG_C
Cuts at: 1180 1180
Size: 4862
NspI r_CATG'y
Cuts at: 450 1789 1915 2415 3322 450
Size: 1339 126 500 907 1990
Fragments arranged by size:
1990 1339 907 500 126
PciI A'CATG_T
Cuts at: 1785 3318 1785
Size: 1533 3329
PflMI CCAn_nnn'nTGG
Cuts at: 184 1445 184
Size: 1261 3601
PmeI GTTT'AAAC
Cuts at: 1861 1861
Size: 4862
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4001 4033 4001
Size: 32 4830
PpuMI rG'GwC_Cy
Cuts at: 1797 1797
Size: 4862
PstI C_TGCA'G
Cuts at: 1298 1909 2594 1298
Size: 611 685 3566
Fragments arranged by size:
3566 685 611
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4862
PvuII CAG'CTG
Cuts at: 2287 2647 2287
Size: 360 4502
RsrII CG'GwC_CG
Cuts at: 3057 3057
Size: 4862
SacI G_AGCT'C
Cuts at: 1876 1876
Size: 4862
SalI G'TCGA_C
Cuts at: 1899 1899
Size: 4862
SapI GCTCTTCn'nnn_
Cuts at: 148 2885 3095 148
Size: 2737 210 1915
Fragments arranged by size:
2737 1915 210
SbfI CC_TGCA'GG
Cuts at: 1909 1909
Size: 4862
ScaI AGT'ACT
Cuts at: 4379 4379
Size: 4862
SfcI C'TryA_G
Cuts at: 33 842 1294 1905 2590 3583 3774 33
Size: 809 452 611 685 993 191 1121
Fragments arranged by size:
1121 993 809 685 611 452 191
SfoI GGC'GCC
Cuts at: 2294 2542 2294
Size: 248 4614
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4862
SmaI CCC'GGG
Cuts at: 1884 1884
Size: 4862
SmlI C'TyrA_G
Cuts at: 2017 2932 3424 3686 2017
Size: 915 492 262 3193
Fragments arranged by size:
3193 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4862
SphI G_CATG'C
Cuts at: 450 1915 2415 450
Size: 1465 500 2897
Fragments arranged by size:
2897 1465 500
StuI AGG'CCT
Cuts at: 182 182
Size: 4862
StyI C'CwwG_G
Cuts at: 107 116 183 737 1996 2350 107
Size: 9 67 554 1259 354 2619
Fragments arranged by size:
2619 1259 554 354 67 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 559 2445 2578 3037 3246 559
Size: 1886 133 459 209 2175
Fragments arranged by size:
2175 1886 459 209 133
TatI w'GTAC_w
Cuts at: 725 1754 4377 725
Size: 1029 2623 1210
Fragments arranged by size:
2623 1210 1029
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4379 4411 4379
Size: 32 4830
Tth111I GACn'n_nGTC
Cuts at: 2659 2659
Size: 4862
XbaI T'CTAG_A
Cuts at: 1893 1893
Size: 4862
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 185 1442 2357 185
Size: 1257 915 2690
Fragments arranged by size:
2690 1257 915
XmaI C'CCGG_G
Cuts at: 1882 1882
Size: 4862
XmnI GAAnn'nnTTC
Cuts at: 1424 1424
Size: 4862
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BsiWI BsmBI BssHII BstEII BstXI Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI