Restriction map for
pF1KE1993
(Circular) (Six-base) MAPSORT of: pF1KE1993.seq Check: 8115 from: 1 to: 4829
pF1KE1993 4829 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AatII G_ACGT'C
Cuts at: 323 361 1672 323
Size: 38 1311 3480
Fragments arranged by size:
3480 1311 38
AccI GT'mk_AC
Cuts at: 1867 1867
Size: 4829
Acc65I G'GTAC_C
Cuts at: 1845 1845
Size: 4829
AclI AA'CG_TT
Cuts at: 4669 4669
Size: 4829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2654 3086 3833 4101 2654
Size: 432 747 268 3382
Fragments arranged by size:
3382 747 432 268
AfeI AGC'GCT
Cuts at: 569 569
Size: 4829
AflIII A'CryG_T
Cuts at: 774 2181 3285 4262 774
Size: 1407 1104 977 1341
Fragments arranged by size:
1407 1341 1104 977
AgeI A'CCGG_T
Cuts at: 3217 3217
Size: 4829
AhdI GACnn_n'nnGTC
Cuts at: 495 495
Size: 4829
AleI CACnn'nnGTG
Cuts at: 868 868
Size: 4829
AlwNI CAG_nnn'CTG
Cuts at: 2134 3701 2134
Size: 1567 3262
ApaI G_GGCC'C
Cuts at: 766 766
Size: 4829
ApaLI G'TGCA_C
Cuts at: 3599 3599
Size: 4829
ApoI r'AATT_y
Cuts at: 591 1833 4182 4438 591
Size: 1242 2349 256 982
Fragments arranged by size:
2349 1242 982 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4829
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
AvaI C'yCGr_G
Cuts at: 766 1393 1849 766
Size: 627 456 3746
Fragments arranged by size:
3746 627 456
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1533 1566 1533
Size: 33 4796
BamHI G'GATC_C
Cuts at: 740 1318 1854 740
Size: 578 536 3715
Fragments arranged by size:
3715 578 536
BanI G'GyrC_C
Cuts at: 682 1168 1477 1785 1845 2259 2507 2542 682
Size: 486 309 308 60 414 248 35 2969
Fragments arranged by size:
2969 486 414 309 308 248 60 35
BanII G_rGCy'C
Cuts at: 766 941 1033 1843 2873 766
Size: 175 92 810 1030 2722
Fragments arranged by size:
2722 1030 810 175 92
BbeI G_GCGC'C
Cuts at: 686 1789 2263 2511 686
Size: 1103 474 248 3004
Fragments arranged by size:
3004 1103 474 248
BbsI GAAGACnn'nnnn_
Cuts at: 148 280 1272 148
Size: 132 992 3705
Fragments arranged by size:
3705 992 132
BbvCI CC'TCA_GC
Cuts at: 890 890
Size: 4829
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 703 737 703
Size: 34 4795
BciVI GTATCCnnnnn_n'
Cuts at: 2727 3488 4829 2727
Size: 761 1341 2727
Fragments arranged by size:
2727 1341 761
BclI T'GATC_A
Cuts at: 161 230 345 416 2349 161
Size: 69 115 71 1933 2641
Fragments arranged by size:
2641 1933 115 71 69
BglI GCCn_nnn'nGGC
Cuts at: 1129 1660 1129
Size: 531 4298
BglII A'GATC_T
Cuts at: 2344 4381 2344
Size: 2037 2792
BlpI GC'TnA_GC
Cuts at: 781 1574 1941 781
Size: 793 367 3669
Fragments arranged by size:
3669 793 367
Bme1580I G_kGCm'C
Cuts at: 766 1104 1171 2454 2547 3603 766
Size: 338 67 1283 93 1056 1992
Fragments arranged by size:
1992 1283 1056 338 93 67
BmrI ACTGGGnnnn_n'
Cuts at: 999 1232 2041 2122 2457 4601 999
Size: 233 809 81 335 2144 1227
Fragments arranged by size:
2144 1227 809 335 233 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 306 3196 306
Size: 2890 1939
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 999 1031 1069 1489 1634 999
Size: 32 38 420 145 4194
Fragments arranged by size:
4194 420 145 38 32
Bpu10I CC'TnA_GC
Cuts at: 437 890 437
Size: 453 4376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1047 2005 2884 3376 3674 1047
Size: 958 879 492 298 2202
Fragments arranged by size:
2202 958 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1073 4533 1073
Size: 3460 1369
BsaAI yAC'GTr
Cuts at: 2182 2182
Size: 4829
BsaBI GATnn'nnATC
Cuts at: 6 301 2343 4380 6
Size: 295 2042 2037 455
Fragments arranged by size:
2042 2037 455 295
BsaHI Gr'CG_yC
Cuts at: 320 358 683 1633 1669 1786 2260 2508 3210
Size: 38 325 950 36 117 474 248 702
Cuts at: 3210 4706 320
Size: 1496 443
Fragments arranged by size:
1496 950 702 474 443 325 248 117
38 36
BsaWI w'CCGG_w
Cuts at: 1161 1700 2018 2539 3217 3491 3638 4049 1161
Size: 539 318 521 678 274 147 411 1941
Fragments arranged by size:
1941 678 539 521 411 318 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1177 1207 1632 1662 1177
Size: 30 425 30 4344
Fragments arranged by size:
4344 425 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 281 571 742 808 1198 1647 281
Size: 290 171 66 390 449 3463
Fragments arranged by size:
3463 449 390 290 171 66
BseYI C'CCAG_C
Cuts at: 400 1772 2254 3012 3589 4085 400
Size: 1372 482 758 577 496 1144
Fragments arranged by size:
1372 1144 758 577 496 482
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 1693
Size: 4829
BsiEI CG_ry'CG
Cuts at: 104 153 2371 2417 3625 104
Size: 49 2218 46 1208 1308
Fragments arranged by size:
2218 1308 1208 49 46
BsiHKAI G_wGCw'C
Cuts at: 1740 1843 2621 2811 3603 1740
Size: 103 778 190 792 2966
Fragments arranged by size:
2966 792 778 190 103
BsmBI CGTCTCn'nnnn_
Cuts at: 1676 1676
Size: 4829
Bsp1286I G_dGCh'C
Cuts at: 766 941 1033 1104 1171 1740 1843 2454 2547
Size: 175 92 71 67 569 103 611 93
Cuts at: 2547 2621 2811 2873 3603 766
Size: 74 190 62 730 1992
Fragments arranged by size:
1992 730 611 569 190 175 103 93
92 74 71 67 62
BspEI T'CCGG_A
Cuts at: 1161 1700 4049 1161
Size: 539 2349 1941
Fragments arranged by size:
2349 1941 539
BspHI T'CATG_A
Cuts at: 4005 4005
Size: 4829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1715 1879 2093 2395 2776 1715
Size: 164 214 302 381 3768
Fragments arranged by size:
3768 381 302 214 164
BsrBI CCG'CTC
Cuts at: 50 1188 3121 3175 4659 4824 50
Size: 1138 1933 54 1484 165 55
Fragments arranged by size:
1933 1484 1138 165 55 54
BsrDI GCAATG_nn'
Cuts at: 719 1104 1307 2741 719
Size: 385 203 1434 2807
Fragments arranged by size:
2807 1434 385 203
BsrFI r'CCGG_y
Cuts at: 2827 3008 3217 2827
Size: 181 209 4439
Fragments arranged by size:
4439 209 181
BsrGI T'GTAC_A
Cuts at: 605 605
Size: 4829
BssSI C'ACGA_G
Cuts at: 144 601 1592 3100 3458 144
Size: 457 991 1508 358 1515
Fragments arranged by size:
1515 1508 991 457 358
BstAPI GCAn_nnn'nTGC
Cuts at: 500 1421 2206 500
Size: 921 785 3123
Fragments arranged by size:
3123 921 785
BstBI TT'CG_AA
Cuts at: 3190 3190
Size: 4829
BstYI r'GATC_y
Cuts at: 1 740 747 1318 1854 2321 2344 2679 2925
Size: 739 7 571 536 467 23 335 246
Cuts at: 2925 3937 4023 4035 4381 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 739 571 536 467 449 346 335
246 86 23 12 7
Bsu36I CC'TnA_GG
Cuts at: 791 791
Size: 4829
BtgI C'CryG_G
Cuts at: 1425 1425
Size: 4829
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 816 882 2779 2960 3123 816
Size: 66 1897 181 163 2522
Fragments arranged by size:
2522 1897 181 163 66
BtsI GCAGTG_nn'
Cuts at: 944 1104 1118 2072 944
Size: 160 14 954 3701
Fragments arranged by size:
3701 954 160 14
DraI TTT'AAA
Cuts at: 1828 1828
Size: 4829
DrdI GACnn_nn'nnGTC
Cuts at: 1087 2535 3393 1087
Size: 1448 858 2523
Fragments arranged by size:
2523 1448 858
EaeI y'GGCC_r
Cuts at: 1661 1797 2414 2588 2979 3006 4351 1661
Size: 136 617 174 391 27 1345 2139
Fragments arranged by size:
2139 1345 617 391 174 136 27
EagI C'GGCC_G
Cuts at: 2414 2414
Size: 4829
EarI CTCTTCn'nnn_
Cuts at: 2852 3062 2852
Size: 210 4619
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3037 3347 3493 4639 3037
Size: 310 146 1146 3227
Fragments arranged by size:
3227 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1841 1841
Size: 4829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 999 1031 1069 1489 1634 2654 3086 3833 4101
Size: 32 38 420 145 1020 432 747 268
Cuts at: 4101 999
Size: 1727
Fragments arranged by size:
1727 1020 747 432 420 268 145 38
32
EcoNI CCTnn'n_nnAGG
Cuts at: 1298 1298
Size: 4829
EcoO109I rG'GnC_Cy
Cuts at: 763 1344 1689 1968 763
Size: 581 345 279 3624
Fragments arranged by size:
3624 581 345 279
EcoRI G'AATT_C
Cuts at: 1833 1833
Size: 4829
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1164 2848 1164
Size: 1684 3145
FspI TGC'GCA
Cuts at: 2610 2610
Size: 4829
HaeII r_GCGC'y
Cuts at: 571 686 1789 2263 2511 3533 4512 571
Size: 115 1103 474 248 1022 979 888
Fragments arranged by size:
1103 1022 979 888 474 248 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 138 170 178 210 330 362 481 513 732
Size: 32 8 32 120 32 119 32 219
Cuts at: 732 764 1020 1052 1177 1209 1632 1664 2671
Size: 32 256 32 125 32 423 32 1007
Cuts at: 2671 2701 2703 2733 138
Size: 30 2 30 2234
Fragments arranged by size:
2234 1007 423 256 219 125 120 119
32 32 32 32 32 32 32 32
30 30 8 2
HincII GTy'rAC
Cuts at: 1868 1868
Size: 4829
KasI G'GCGC_C
Cuts at: 682 1785 2259 2507 682
Size: 1103 474 248 3004
Fragments arranged by size:
3004 1103 474 248
KpnI G_GTAC'C
Cuts at: 1849 1849
Size: 4829
MluI A'CGCG_T
Cuts at: 4262 4262
Size: 4829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1063 3500 3684 1063
Size: 2437 184 2208
Fragments arranged by size:
2437 2208 184
MscI TGG'CCA
Cuts at: 2590 2590
Size: 4829
MslI CAynn'nnrTG
Cuts at: 868 1312 2818 2945 868
Size: 444 1506 127 2752
Fragments arranged by size:
2752 1506 444 127
MspA1I CmG'CkG
Cuts at: 855 1427 1940 2254 2614 3627 3872 855
Size: 572 513 314 360 1013 245 1812
Fragments arranged by size:
1812 1013 572 513 360 314 245
NaeI GCC'GGC
Cuts at: 3010 3010
Size: 4829
NarI GG'CG_CC
Cuts at: 683 1786 2260 2508 683
Size: 1103 474 248 3004
Fragments arranged by size:
3004 1103 474 248
NgoMIV G'CCGG_C
Cuts at: 3008 3008
Size: 4829
NspI r_CATG'y
Cuts at: 523 778 1489 1882 2382 3289 523
Size: 255 711 393 500 907 2063
Fragments arranged by size:
2063 907 711 500 393 255
PciI A'CATG_T
Cuts at: 774 3285 774
Size: 2511 2318
PflMI CCAn_nnn'nTGG
Cuts at: 1248 1248
Size: 4829
PfoI T'CCnGG_A
Cuts at: 292 551 671 743 1466 292
Size: 259 120 72 723 3655
Fragments arranged by size:
3655 723 259 120 72
PmeI GTTT'AAAC
Cuts at: 1828 1828
Size: 4829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 4797
PpuMI rG'GwC_Cy
Cuts at: 1344 1344
Size: 4829
PspOMI G'GGCC_C
Cuts at: 762 762
Size: 4829
PstI C_TGCA'G
Cuts at: 1876 2561 1876
Size: 685 4144
PvuI CG_AT'CG
Cuts at: 104 153 104
Size: 49 4780
PvuII CAG'CTG
Cuts at: 2254 2614 2254
Size: 360 4469
RsrII CG'GwC_CG
Cuts at: 3024 3024
Size: 4829
SacI G_AGCT'C
Cuts at: 1843 1843
Size: 4829
SacII CC_GC'GG
Cuts at: 1428 1428
Size: 4829
SalI G'TCGA_C
Cuts at: 1866 1866
Size: 4829
SanDI GG'GwC_CC
Cuts at: 1344 1344
Size: 4829
SapI GCTCTTCn'nnn_
Cuts at: 2852 3062 2852
Size: 210 4619
SbfI CC_TGCA'GG
Cuts at: 1876 1876
Size: 4829
ScaI AGT'ACT
Cuts at: 659 4346 659
Size: 3687 1142
SexAI A'CCwGG_T
Cuts at: 1331 1331
Size: 4829
SfcI C'TryA_G
Cuts at: 33 1133 1872 2557 3550 3741 33
Size: 1100 739 685 993 191 1121
Fragments arranged by size:
1121 1100 993 739 685 191
SfoI GGC'GCC
Cuts at: 684 1787 2261 2509 684
Size: 1103 474 248 3004
Fragments arranged by size:
3004 1103 474 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
SmaI CCC'GGG
Cuts at: 1851 1851
Size: 4829
SmlI C'TyrA_G
Cuts at: 1026 1393 1984 2899 3391 3653 1026
Size: 367 591 915 492 262 2202
Fragments arranged by size:
2202 915 591 492 367 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4829
SphI G_CATG'C
Cuts at: 1882 2382 1882
Size: 500 4329
SspI AAT'ATT
Cuts at: 132 132
Size: 4829
StyI C'CwwG_G
Cuts at: 378 386 866 995 1963 2317 378
Size: 8 480 129 968 354 2890
Fragments arranged by size:
2890 968 480 354 129 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1786 2412 2545 3004 3213 1786
Size: 626 133 459 209 3402
Fragments arranged by size:
3402 626 459 209 133
TatI w'GTAC_w
Cuts at: 605 657 1397 4344 605
Size: 52 740 2947 1090
Fragments arranged by size:
2947 1090 740 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 911 943 1143 1175 4346 4378 911
Size: 32 200 32 3171 32 1362
Fragments arranged by size:
3171 1362 200 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1333 2626 1333
Size: 1293 3536
XbaI T'CTAG_A
Cuts at: 1860 1860
Size: 4829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 393 706 2324 393
Size: 313 1618 2898
Fragments arranged by size:
2898 1618 313
XhoI C'TCGA_G
Cuts at: 1393 1393
Size: 4829
XmaI C'CCGG_G
Cuts at: 1849 1849
Size: 4829
XmnI GAAnn'nnTTC
Cuts at: 1525 1525
Size: 4829
ZraI GAC'GTC
Cuts at: 321 359 1670 321
Size: 38 1311 3480
Fragments arranged by size:
3480 1311 38
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AlfI AloI AscI AvrII BfrBI BmgBI
BmtI BsiWI BsmI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI
SrfI StuI SwaI