Restriction map for
pF1KE1971
(Circular) (Six-base) MAPSORT of: pF1KE1971.seq Check: 7957 from: 1 to: 4727
pF1KE1971 4727 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1389 1765 1389
Size: 376 4351
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 490 538 820 1637 2552 2984 3731 3999
Size: 214 48 282 817 915 432 747 268
Cuts at: 3999 276
Size: 1004
Fragments arranged by size:
1004 915 817 747 432 282 268 214
48
AflIII A'CryG_T
Cuts at: 2079 3183 4160 2079
Size: 1104 977 2646
Fragments arranged by size:
2646 1104 977
AgeI A'CCGG_T
Cuts at: 3115 3115
Size: 4727
AlwNI CAG_nnn'CTG
Cuts at: 1569 2032 3599 1569
Size: 463 1567 2697
Fragments arranged by size:
2697 1567 463
ApaLI G'TGCA_C
Cuts at: 3497 3497
Size: 4727
ApoI r'AATT_y
Cuts at: 575 823 868 1731 4080 4336 575
Size: 248 45 863 2349 256 966
Fragments arranged by size:
2349 966 863 256 248 45
AseI AT'TA_AT
Cuts at: 20 491 20
Size: 471 4256
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 980 1586 1747 980
Size: 606 161 3960
Fragments arranged by size:
3960 606 161
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 237 1743 2157 2405 2440 237
Size: 1506 414 248 35 2524
Fragments arranged by size:
2524 1506 414 248 35
BanII G_rGCy'C
Cuts at: 573 1741 2771 573
Size: 1168 1030 2529
Fragments arranged by size:
2529 1168 1030
BbeI G_GCGC'C
Cuts at: 2161 2409 2161
Size: 248 4479
BbsI GAAGACnn'nnnn_
Cuts at: 1701 1701
Size: 4727
BbvCI CC'TCA_GC
Cuts at: 1676 1676
Size: 4727
BciVI GTATCCnnnnn_n'
Cuts at: 979 1234 2625 3386 4727 979
Size: 255 1391 761 1341 979
Fragments arranged by size:
1391 1341 979 761 255
BclI T'GATC_A
Cuts at: 995 2247 995
Size: 1252 3475
BglII A'GATC_T
Cuts at: 336 2242 4279 336
Size: 1906 2037 784
Fragments arranged by size:
2037 1906 784
BlpI GC'TnA_GC
Cuts at: 191 1839 191
Size: 1648 3079
Bme1580I G_kGCm'C
Cuts at: 780 1040 1321 2352 2445 3501 780
Size: 260 281 1031 93 1056 2006
Fragments arranged by size:
2006 1056 1031 281 260 93
BmrI ACTGGGnnnn_n'
Cuts at: 1939 2020 2355 4499 1939
Size: 81 335 2144 2167
Fragments arranged by size:
2167 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 684 3094 684
Size: 2410 2317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 4727
Bpu10I CC'TnA_GC
Cuts at: 358 1676 358
Size: 1318 3409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 1903 2782 3274 3572 334
Size: 1569 879 492 298 1489
Fragments arranged by size:
1569 1489 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4431 4431
Size: 4727
BsaAI yAC'GTr
Cuts at: 198 2080 198
Size: 1882 2845
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 2158 2406 3108 4604 2158
Size: 248 702 1496 2281
Fragments arranged by size:
2281 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1916 2437 3115 3389 3536 3947 1916
Size: 521 678 274 147 411 2696
Fragments arranged by size:
2696 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 874 904 874
Size: 30 4697
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 898 1426 550
Size: 348 528 3851
Fragments arranged by size:
3851 528 348
BseYI C'CCAG_C
Cuts at: 1485 2152 2910 3487 3983 1485
Size: 667 758 577 496 2229
Fragments arranged by size:
2229 758 667 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1128 1128
Size: 4727
BsiEI CG_ry'CG
Cuts at: 104 2269 2315 3523 104
Size: 2165 46 1208 1308
Fragments arranged by size:
2165 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 197 1741 2519 2709 3501 197
Size: 1544 778 190 792 1423
Fragments arranged by size:
1544 1423 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 197 573 780 1040 1321 1741 2352 2445 2519
Size: 376 207 260 281 420 611 93 74
Cuts at: 2519 2709 2771 3501 197
Size: 190 62 730 1423
Fragments arranged by size:
1423 730 611 420 376 281 260 207
190 93 74 62
BspEI T'CCGG_A
Cuts at: 3947 3947
Size: 4727
BspHI T'CATG_A
Cuts at: 3903 3903
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 200 707 1777 1991 2293 2674 200
Size: 507 1070 214 302 381 2253
Fragments arranged by size:
2253 1070 507 381 302 214
BsrBI CCG'CTC
Cuts at: 50 256 3019 3073 4557 4722 50
Size: 206 2763 54 1484 165 55
Fragments arranged by size:
2763 1484 206 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2639 2639
Size: 4727
BsrFI r'CCGG_y
Cuts at: 2725 2906 3115 2725
Size: 181 209 4337
Fragments arranged by size:
4337 209 181
BssSI C'ACGA_G
Cuts at: 2998 3356 2998
Size: 358 4369
BstAPI GCAn_nnn'nTGC
Cuts at: 2104 2104
Size: 4727
BstBI TT'CG_AA
Cuts at: 3088 3088
Size: 4727
BstYI r'GATC_y
Cuts at: 1 336 1752 2219 2242 2577 2823 3835 3921
Size: 335 1416 467 23 335 246 1012 86
Cuts at: 3921 3933 4279 1
Size: 12 346 449
Fragments arranged by size:
1416 1012 467 449 346 335 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1390 1390
Size: 4727
BtgI C'CryG_G
Cuts at: 107 1233 107
Size: 1126 3601
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 247 1593 1607 2677 2858 3021 247
Size: 1346 14 1070 181 163 1953
Fragments arranged by size:
1953 1346 1070 181 163 14
BtsI GCAGTG_nn'
Cuts at: 1970 1970
Size: 4727
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1086 1121 1086
Size: 35 4692
DraI TTT'AAA
Cuts at: 279 1726 279
Size: 1447 3280
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3291 2433
Size: 858 3869
EaeI y'GGCC_r
Cuts at: 1493 2312 2486 2877 2904 4249 1493
Size: 819 174 391 27 1345 1971
Fragments arranged by size:
1971 1345 819 391 174 27
EagI C'GGCC_G
Cuts at: 2312 2312
Size: 4727
EarI CTCTTCn'nnn_
Cuts at: 117 487 721 1103 2750 2960 117
Size: 370 234 382 1647 210 1884
Fragments arranged by size:
1884 1647 382 370 234 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2935 3245 3391 4537 2935
Size: 310 146 1146 3125
Fragments arranged by size:
3125 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1739 1739
Size: 4727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 412 490 538 820 1637 2552 2984 3731
Size: 136 78 48 282 817 915 432 747
Cuts at: 3731 3999 276
Size: 268 1004
Fragments arranged by size:
1004 915 817 747 432 282 268 136
78 48
EcoO109I rG'GnC_Cy
Cuts at: 1275 1866 1275
Size: 591 4136
EcoRI G'AATT_C
Cuts at: 823 1731 823
Size: 908 3819
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 306 423 2746 306
Size: 117 2323 2287
Fragments arranged by size:
2323 2287 117
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 2161 2409 3431 4410 2161
Size: 248 1022 979 2478
Fragments arranged by size:
2478 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 328 360 922 954 1279 1311 1426 1458 2569
Size: 32 562 32 325 32 115 32 1111
Cuts at: 2569 2599 2601 2631 328
Size: 30 2 30 2424
Fragments arranged by size:
2424 1111 562 325 115 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1766 1766
Size: 4727
KasI G'GCGC_C
Cuts at: 2157 2405 2157
Size: 248 4479
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 425 734 3398 3582 425
Size: 309 2664 184 1570
Fragments arranged by size:
2664 1570 309 184
MscI TGG'CCA
Cuts at: 1495 2488 1495
Size: 993 3734
MslI CAynn'nnrTG
Cuts at: 718 1079 2716 2843 718
Size: 361 1637 127 2602
Fragments arranged by size:
2602 1637 361 127
MspA1I CmG'CkG
Cuts at: 1838 2152 2512 3525 3770 1838
Size: 314 360 1013 245 2795
Fragments arranged by size:
2795 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2908 2908
Size: 4727
NarI GG'CG_CC
Cuts at: 2158 2406 2158
Size: 248 4479
NcoI C'CATG_G
Cuts at: 107 1233 107
Size: 1126 3601
NgoMIV G'CCGG_C
Cuts at: 2906 2906
Size: 4727
NspI r_CATG'y
Cuts at: 1780 2280 3187 1780
Size: 500 907 3320
Fragments arranged by size:
3320 907 500
PciI A'CATG_T
Cuts at: 3183 3183
Size: 4727
PflMI CCAn_nnn'nTGG
Cuts at: 1188 1188
Size: 4727
PfoI T'CCnGG_A
Cuts at: 428 1463 428
Size: 1035 3692
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PmlI CAC'GTG
Cuts at: 198 198
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4695
PspXI vC'TCGA_Gb
Cuts at: 1586 1586
Size: 4727
PstI C_TGCA'G
Cuts at: 1533 1774 2459 1533
Size: 241 685 3801
Fragments arranged by size:
3801 685 241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 2152 2512 2152
Size: 360 4367
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1741 1741
Size: 4727
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 117 487 1103 2750 2960 117
Size: 370 616 1647 210 1884
Fragments arranged by size:
1884 1647 616 370 210
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SexAI A'CCwGG_T
Cuts at: 1540 1540
Size: 4727
SfcI C'TryA_G
Cuts at: 33 585 1529 1770 2455 3448 3639 33
Size: 552 944 241 685 993 191 1121
Fragments arranged by size:
1121 993 944 685 552 241 191
SfoI GGC'GCC
Cuts at: 2159 2407 2159
Size: 248 4479
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 1749 1749
Size: 4727
SmlI C'TyrA_G
Cuts at: 349 1586 1882 2797 3289 3551 349
Size: 1237 296 915 492 262 1525
Fragments arranged by size:
1525 1237 915 492 296 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 1780 2280 1780
Size: 500 4227
StyI C'CwwG_G
Cuts at: 107 1233 1861 2215 107
Size: 1126 628 354 2619
Fragments arranged by size:
2619 1126 628 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2310 2443 2902 3111 2310
Size: 133 459 209 3926
Fragments arranged by size:
3926 459 209 133
TatI w'GTAC_w
Cuts at: 4242 4242
Size: 4727
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 874 906 4244 4276 874
Size: 32 3338 32 1325
Fragments arranged by size:
3338 1325 32 32
Tth111I GACn'n_nGTC
Cuts at: 2524 2524
Size: 4727
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 678 2222 678
Size: 1544 3183
XhoI C'TCGA_G
Cuts at: 1586 1586
Size: 4727
XmaI C'CCGG_G
Cuts at: 1747 1747
Size: 4727
XmnI GAAnn'nnTTC
Cuts at: 1112 1112
Size: 4727
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PpuMI PshAI PsiI PspOMI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI