Restriction map for
pF1KE1875
(Circular) (Six-base) MAPSORT of: pF1KE1875.seq Check: 283 from: 1 to: 4307
pF1KE1875 4307 bp
With 182 enzymes: SgfI *
July 27, 2017 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 841 841
Size: 4307
AccI GT'mk_AC
Cuts at: 1267 1345 1267
Size: 78 4229
Acc65I G'GTAC_C
Cuts at: 1323 1323
Size: 4307
AclI AA'CG_TT
Cuts at: 4147 4147
Size: 4307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2132 2564 3311 3579 2132
Size: 432 747 268 2860
Fragments arranged by size:
2860 747 432 268
AflIII A'CryG_T
Cuts at: 1659 2763 3740 1659
Size: 1104 977 2226
Fragments arranged by size:
2226 1104 977
AgeI A'CCGG_T
Cuts at: 2695 2695
Size: 4307
AlwNI CAG_nnn'CTG
Cuts at: 620 726 1242 1612 3179 620
Size: 106 516 370 1567 1748
Fragments arranged by size:
1748 1567 516 370 106
ApaI G_GGCC'C
Cuts at: 354 526 354
Size: 172 4135
ApaLI G'TGCA_C
Cuts at: 3077 3077
Size: 4307
ApoI r'AATT_y
Cuts at: 1311 3660 3916 1311
Size: 2349 256 1702
Fragments arranged by size:
2349 1702 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4307
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
AvaI C'yCGr_G
Cuts at: 393 1327 393
Size: 934 3373
BamHI G'GATC_C
Cuts at: 1332 1332
Size: 4307
BanI G'GyrC_C
Cuts at: 373 1323 1737 1985 2020 373
Size: 950 414 248 35 2660
Fragments arranged by size:
2660 950 414 248 35
BanII G_rGCy'C
Cuts at: 338 354 526 566 1321 2351 338
Size: 16 172 40 755 1030 2294
Fragments arranged by size:
2294 1030 755 172 40 16
BbeI G_GCGC'C
Cuts at: 377 1741 1989 377
Size: 1364 248 2695
Fragments arranged by size:
2695 1364 248
BciVI GTATCCnnnnn_n'
Cuts at: 2205 2966 4307 2205
Size: 761 1341 2205
Fragments arranged by size:
2205 1341 761
BclI T'GATC_A
Cuts at: 1827 1827
Size: 4307
BglI GCCn_nnn'nGGC
Cuts at: 398 398
Size: 4307
BglII A'GATC_T
Cuts at: 1822 3859 1822
Size: 2037 2270
BlpI GC'TnA_GC
Cuts at: 782 1419 782
Size: 637 3670
Bme1580I G_kGCm'C
Cuts at: 354 467 526 1932 2025 3081 354
Size: 113 59 1406 93 1056 1580
Fragments arranged by size:
1580 1406 1056 113 93 59
BmgBI CAC'GTC
Cuts at: 852 852
Size: 4307
BmrI ACTGGGnnnn_n'
Cuts at: 406 454 700 1519 1600 1935 4079 406
Size: 48 246 819 81 335 2144 634
Fragments arranged by size:
2144 819 634 335 246 81 48
BmtI G_CTAG'C
Cuts at: 443 443
Size: 4307
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 4307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 580 390
Size: 190 4117
Bpu10I CC'TnA_GC
Cuts at: 130 247 130
Size: 117 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1122 1483 2362 2854 3152 664
Size: 458 361 879 492 298 1819
Fragments arranged by size:
1819 879 492 458 361 298
BsaI GGTCTCn'nnnn_
Cuts at: 4011 4011
Size: 4307
BsaAI yAC'GTr
Cuts at: 1660 1660
Size: 4307
BsaBI GATnn'nnATC
Cuts at: 6 1821 3858 6
Size: 1815 2037 455
Fragments arranged by size:
2037 1815 455
BsaHI Gr'CG_yC
Cuts at: 212 374 1738 1986 2688 4184 212
Size: 162 1364 248 702 1496 335
Fragments arranged by size:
1496 1364 702 335 248 162
BsaWI w'CCGG_w
Cuts at: 338 343 1496 2017 2695 2969 3116 3527 338
Size: 5 1153 521 678 274 147 411 1118
Fragments arranged by size:
1153 1118 678 521 411 274 147 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 356 369 517 620 778 1055 356
Size: 13 148 103 158 277 3608
Fragments arranged by size:
3608 277 158 148 103 13
BseYI C'CCAG_C
Cuts at: 383 1034 1732 2490 3067 3563 383
Size: 651 698 758 577 496 1127
Fragments arranged by size:
1127 758 698 651 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 187
Size: 4307
BsiEI CG_ry'CG
Cuts at: 104 358 603 1849 1895 3103 104
Size: 254 245 1246 46 1208 1308
Fragments arranged by size:
1308 1246 1208 254 245 46
BsiHKAI G_wGCw'C
Cuts at: 338 566 677 1321 2099 2289 3081 338
Size: 228 111 644 778 190 792 1564
Fragments arranged by size:
1564 792 778 644 228 190 111
BsmBI CGTCTCn'nnnn_
Cuts at: 630 630
Size: 4307
Bsp1286I G_dGCh'C
Cuts at: 338 354 467 526 566 677 1321 1932 2025
Size: 16 113 59 40 111 644 611 93
Cuts at: 2025 2099 2289 2351 3081 338
Size: 74 190 62 730 1564
Fragments arranged by size:
1564 730 644 611 190 113 111 93
74 62 59 40 16
BspEI T'CCGG_A
Cuts at: 338 3527 338
Size: 3189 1118
BspHI T'CATG_A
Cuts at: 3483 3483
Size: 4307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 1357 1571 1873 2254 841
Size: 516 214 302 381 2894
Fragments arranged by size:
2894 516 381 302 214
BsrBI CCG'CTC
Cuts at: 50 126 134 2599 2653 4137 4302 50
Size: 76 8 2465 54 1484 165 55
Fragments arranged by size:
2465 1484 165 76 55 54 8
BsrDI GCAATG_nn'
Cuts at: 929 2219 929
Size: 1290 3017
BsrFI r'CCGG_y
Cuts at: 665 2305 2486 2695 665
Size: 1640 181 209 2277
Fragments arranged by size:
2277 1640 209 181
BsrGI T'GTAC_A
Cuts at: 1248 1248
Size: 4307
BssSI C'ACGA_G
Cuts at: 184 565 2578 2936 184
Size: 381 2013 358 1555
Fragments arranged by size:
2013 1555 381 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1684 1684
Size: 4307
BstBI TT'CG_AA
Cuts at: 2668 2668
Size: 4307
BstYI r'GATC_y
Cuts at: 1 1332 1799 1822 2157 2403 3415 3501 3513
Size: 1331 467 23 335 246 1012 86 12
Cuts at: 3513 3859 1
Size: 346 449
Fragments arranged by size:
1331 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1268 1268
Size: 4307
Bsu36I CC'TnA_GG
Cuts at: 500 500
Size: 4307
BtgI C'CryG_G
Cuts at: 904 904
Size: 4307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 160 2257 2438 2601 160
Size: 2097 181 163 1866
Fragments arranged by size:
2097 1866 181 163
BtsI GCAGTG_nn'
Cuts at: 1550 1550
Size: 4307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1088 1123 1088
Size: 35 4272
DraI TTT'AAA
Cuts at: 1306 1306
Size: 4307
DraIII CAC_nnn'GTG
Cuts at: 639 639
Size: 4307
DrdI GACnn_nn'nnGTC
Cuts at: 2013 2871 2013
Size: 858 3449
EaeI y'GGCC_r
Cuts at: 188 237 355 600 641 1892 2066 2457 2484
Size: 49 118 245 41 1251 174 391 27
Cuts at: 2484 3829 188
Size: 1345 666
Fragments arranged by size:
1345 1251 666 391 245 174 118 49
41 27
EagI C'GGCC_G
Cuts at: 355 600 1892 355
Size: 245 1292 2770
Fragments arranged by size:
2770 1292 245
EarI CTCTTCn'nnn_
Cuts at: 2330 2540 2330
Size: 210 4097
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1120 2515 2825 2971 4117 1120
Size: 1395 310 146 1146 1310
Fragments arranged by size:
1395 1310 1146 310 146
EcoICRI GAG'CTC
Cuts at: 336 564 1319 336
Size: 228 755 3324
Fragments arranged by size:
3324 755 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 580 2132 2564 3311 3579 390
Size: 190 1552 432 747 268 1118
Fragments arranged by size:
1552 1118 747 432 268 190
EcoNI CCTnn'n_nnAGG
Cuts at: 368 368
Size: 4307
EcoO109I rG'GnC_Cy
Cuts at: 350 523 1446 350
Size: 173 923 3211
Fragments arranged by size:
3211 923 173
EcoRI G'AATT_C
Cuts at: 1311 1311
Size: 4307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 4307
FspI TGC'GCA
Cuts at: 2088 2088
Size: 4307
HaeII r_GCGC'y
Cuts at: 143 377 720 1741 1989 3011 3990 143
Size: 234 343 1021 248 1022 979 460
Fragments arranged by size:
1022 1021 979 460 343 248 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 610 642 2149 2179 2181 2211 610
Size: 32 1507 30 2 30 2706
Fragments arranged by size:
2706 1507 32 30 30 2
HincII GTy'rAC
Cuts at: 1346 1346
Size: 4307
KasI G'GCGC_C
Cuts at: 373 1737 1985 373
Size: 1364 248 2695
Fragments arranged by size:
2695 1364 248
KpnI G_GTAC'C
Cuts at: 1327 1327
Size: 4307
MluI A'CGCG_T
Cuts at: 3740 3740
Size: 4307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 249 2978 3162 249
Size: 2729 184 1394
Fragments arranged by size:
2729 1394 184
MscI TGG'CCA
Cuts at: 643 2068 643
Size: 1425 2882
MslI CAynn'nnrTG
Cuts at: 550 865 2296 2423 550
Size: 315 1431 127 2434
Fragments arranged by size:
2434 1431 315 127
MspA1I CmG'CkG
Cuts at: 168 285 513 726 1418 1732 2092 3105 3350
Size: 117 228 213 692 314 360 1013 245
Cuts at: 3350 168
Size: 1125
Fragments arranged by size:
1125 1013 692 360 314 245 228 213
117
NaeI GCC'GGC
Cuts at: 2488 2488
Size: 4307
NarI GG'CG_CC
Cuts at: 374 1738 1986 374
Size: 1364 248 2695
Fragments arranged by size:
2695 1364 248
NgoMIV G'CCGG_C
Cuts at: 2486 2486
Size: 4307
NheI G'CTAG_C
Cuts at: 439 439
Size: 4307
NotI GC'GGCC_GC
Cuts at: 600 600
Size: 4307
NspI r_CATG'y
Cuts at: 549 1086 1360 1860 2767 549
Size: 537 274 500 907 2089
Fragments arranged by size:
2089 907 537 500 274
PasI CC'CwG_GG
Cuts at: 614 614
Size: 4307
PciI A'CATG_T
Cuts at: 2763 2763
Size: 4307
PflMI CCAn_nnn'nTGG
Cuts at: 726 1242 726
Size: 516 3791
PmeI GTTT'AAAC
Cuts at: 1306 1306
Size: 4307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 4275
PshAI GACnn'nnGTC
Cuts at: 454 454
Size: 4307
PspOMI G'GGCC_C
Cuts at: 350 522 350
Size: 172 4135
PstI C_TGCA'G
Cuts at: 290 716 1354 2039 290
Size: 426 638 685 2558
Fragments arranged by size:
2558 685 638 426
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4307
PvuII CAG'CTG
Cuts at: 726 1732 2092 726
Size: 1006 360 2941
Fragments arranged by size:
2941 1006 360
RsrII CG'GwC_CG
Cuts at: 341 2502 341
Size: 2161 2146
SacI G_AGCT'C
Cuts at: 338 566 1321 338
Size: 228 755 3324
Fragments arranged by size:
3324 755 228
SalI G'TCGA_C
Cuts at: 1344 1344
Size: 4307
SapI GCTCTTCn'nnn_
Cuts at: 2330 2540 2330
Size: 210 4097
SbfI CC_TGCA'GG
Cuts at: 1354 1354
Size: 4307
ScaI AGT'ACT
Cuts at: 685 3824 685
Size: 3139 1168
SexAI A'CCwGG_T
Cuts at: 1235 1235
Size: 4307
SfcI C'TryA_G
Cuts at: 33 163 286 712 1350 2035 3028 3219 33
Size: 130 123 426 638 685 993 191 1121
Fragments arranged by size:
1121 993 685 638 426 191 130 123
SfoI GGC'GCC
Cuts at: 375 1739 1987 375
Size: 1364 248 2695
Fragments arranged by size:
2695 1364 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
SmaI CCC'GGG
Cuts at: 395 1329 395
Size: 934 3373
SmlI C'TyrA_G
Cuts at: 679 1137 1462 2377 2869 3131 679
Size: 458 325 915 492 262 1855
Fragments arranged by size:
1855 915 492 458 325 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4307
SphI G_CATG'C
Cuts at: 1360 1860 1360
Size: 500 3807
StyI C'CwwG_G
Cuts at: 207 1441 1795 207
Size: 1234 354 2719
Fragments arranged by size:
2719 1234 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 444 719 1193 1890 2023 2482 2691 444
Size: 275 474 697 133 459 209 2060
Fragments arranged by size:
2060 697 474 459 275 209 133
TatI w'GTAC_w
Cuts at: 683 1248 3822 683
Size: 565 2574 1168
Fragments arranged by size:
2574 1168 565
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3824 3856 3824
Size: 32 4275
Tth111I GACn'n_nGTC
Cuts at: 622 2104 622
Size: 1482 2825
XbaI T'CTAG_A
Cuts at: 1338 1338
Size: 4307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 414 1802 414
Size: 1388 2919
XmaI C'CCGG_G
Cuts at: 393 1327 393
Size: 934 3373
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BsaXI BsiWI
BsmI BssHII BstEII BstXI ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NruI NsiI PacI
PfoI PmlI PpuMI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI