Restriction map for
pF1KE1823
(Circular) (Six-base) MAPSORT of: pF1KE1823.seq Check: 5964 from: 1 to: 4121
pF1KE1823 4121 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 146 1159 146
Size: 1013 3108
Acc65I G'GTAC_C
Cuts at: 1137 1137
Size: 4121
AclI AA'CG_TT
Cuts at: 3961 3961
Size: 4121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 1946 2378 3125 3393 425
Size: 1521 432 747 268 1153
Fragments arranged by size:
1521 1153 747 432 268
AflIII A'CryG_T
Cuts at: 203 1473 2577 3554 203
Size: 1270 1104 977 770
Fragments arranged by size:
1270 1104 977 770
AgeI A'CCGG_T
Cuts at: 1024 2509 1024
Size: 1485 2636
AlwNI CAG_nnn'CTG
Cuts at: 1426 2993 1426
Size: 1567 2554
ApaLI G'TGCA_C
Cuts at: 2891 2891
Size: 4121
ApoI r'AATT_y
Cuts at: 354 663 1125 3474 3730 354
Size: 309 462 2349 256 745
Fragments arranged by size:
2349 745 462 309 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4121
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
AvaI C'yCGr_G
Cuts at: 756 1141 756
Size: 385 3736
BamHI G'GATC_C
Cuts at: 1146 1146
Size: 4121
BanI G'GyrC_C
Cuts at: 993 1137 1551 1799 1834 993
Size: 144 414 248 35 3280
Fragments arranged by size:
3280 414 248 144 35
BanII G_rGCy'C
Cuts at: 156 1135 2165 156
Size: 979 1030 2112
Fragments arranged by size:
2112 1030 979
BbeI G_GCGC'C
Cuts at: 1555 1803 1555
Size: 248 3873
BciVI GTATCCnnnnn_n'
Cuts at: 233 975 2019 2780 4121 233
Size: 742 1044 761 1341 233
Fragments arranged by size:
1341 1044 761 742 233
BclI T'GATC_A
Cuts at: 1641 1641
Size: 4121
BglII A'GATC_T
Cuts at: 960 1636 3673 960
Size: 676 2037 1408
Fragments arranged by size:
2037 1408 676
BlpI GC'TnA_GC
Cuts at: 1233 1233
Size: 4121
Bme1580I G_kGCm'C
Cuts at: 1746 1839 2895 1746
Size: 93 1056 2972
Fragments arranged by size:
2972 1056 93
BmgBI CAC'GTC
Cuts at: 578 578
Size: 4121
BmrI ACTGGGnnnn_n'
Cuts at: 1333 1414 1749 3893 1333
Size: 81 335 2144 1561
Fragments arranged by size:
2144 1561 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 890
Size: 4121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 2176 2668 2966 1297
Size: 879 492 298 2452
Fragments arranged by size:
2452 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3825 3825
Size: 4121
BsaAI yAC'GTr
Cuts at: 1474 1474
Size: 4121
BsaBI GATnn'nnATC
Cuts at: 6 1635 3672 6
Size: 1629 2037 455
Fragments arranged by size:
2037 1629 455
BsaHI Gr'CG_yC
Cuts at: 1552 1800 2502 3998 1552
Size: 248 702 1496 1675
Fragments arranged by size:
1675 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1024 1310 1831 2509 2783 2930 3341 1024
Size: 286 521 678 274 147 411 1804
Fragments arranged by size:
1804 678 521 411 286 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 863 893 863
Size: 30 4091
BseYI C'CCAG_C
Cuts at: 272 1546 2304 2881 3377 272
Size: 1274 758 577 496 1016
Fragments arranged by size:
1274 1016 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 607
Size: 4121
BsiEI CG_ry'CG
Cuts at: 104 1663 1709 2917 104
Size: 1559 46 1208 1308
Fragments arranged by size:
1559 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 156 1135 1913 2103 2895 156
Size: 979 778 190 792 1382
Fragments arranged by size:
1382 979 792 778 190
BsmI GAATG_Cn'
Cuts at: 402 402
Size: 4121
Bsp1286I G_dGCh'C
Cuts at: 156 1135 1746 1839 1913 2103 2165 2895 156
Size: 979 611 93 74 190 62 730 1382
Fragments arranged by size:
1382 979 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3341 3341
Size: 4121
BspHI T'CATG_A
Cuts at: 3297 3297
Size: 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1171 1385 1687 2068 1171
Size: 214 302 381 3224
Fragments arranged by size:
3224 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2413 2467 3951 4116 50
Size: 2363 54 1484 165 55
Fragments arranged by size:
2363 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2033 2033
Size: 4121
BsrFI r'CCGG_y
Cuts at: 1024 2119 2300 2509 1024
Size: 1095 181 209 2636
Fragments arranged by size:
2636 1095 209 181
BssSI C'ACGA_G
Cuts at: 2392 2750 2392
Size: 358 3763
BstAPI GCAn_nnn'nTGC
Cuts at: 1498 1498
Size: 4121
BstBI TT'CG_AA
Cuts at: 2482 2482
Size: 4121
BstYI r'GATC_y
Cuts at: 1 608 960 1146 1613 1636 1971 2217 3229
Size: 607 352 186 467 23 335 246 1012
Cuts at: 3229 3315 3327 3673 1
Size: 86 12 346 449
Fragments arranged by size:
1012 607 467 449 352 346 335 246
186 86 23 12
BstZ17I GTA'TAC
Cuts at: 147 147
Size: 4121
BtgI C'CryG_G
Cuts at: 107 654 708 1046 107
Size: 547 54 338 3182
Fragments arranged by size:
3182 547 338 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2071 2252 2415 2071
Size: 181 163 3777
Fragments arranged by size:
3777 181 163
BtsI GCAGTG_nn'
Cuts at: 599 1364 599
Size: 765 3356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 900 935 900
Size: 35 4086
DraI TTT'AAA
Cuts at: 1120 1120
Size: 4121
DrdI GACnn_nn'nnGTC
Cuts at: 1827 2685 1827
Size: 858 3263
EaeI y'GGCC_r
Cuts at: 1706 1880 2271 2298 3643 1706
Size: 174 391 27 1345 2184
Fragments arranged by size:
2184 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1706 1706
Size: 4121
EarI CTCTTCn'nnn_
Cuts at: 140 158 951 2144 2354 140
Size: 18 793 1193 210 1907
Fragments arranged by size:
1907 1193 793 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2329 2639 2785 3931 2329
Size: 310 146 1146 2519
Fragments arranged by size:
2519 1146 310 146
EcoICRI GAG'CTC
Cuts at: 154 1133 154
Size: 979 3142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 890 1946 2378 3125 3393 425
Size: 465 1056 432 747 268 1153
Fragments arranged by size:
1153 1056 747 465 432 268
EcoO109I rG'GnC_Cy
Cuts at: 269 1260 269
Size: 991 3130
EcoRI G'AATT_C
Cuts at: 354 1125 354
Size: 771 3350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 187 2140 187
Size: 1953 2168
FspI TGC'GCA
Cuts at: 1902 1902
Size: 4121
HaeII r_GCGC'y
Cuts at: 1555 1803 2825 3804 1555
Size: 248 1022 979 1872
Fragments arranged by size:
1872 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 945 977 1963 1993 1995 2025 190
Size: 32 723 32 986 30 2 30 2286
Fragments arranged by size:
2286 986 723 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1030 1077 1160 1030
Size: 47 83 3991
Fragments arranged by size:
3991 83 47
KasI G'GCGC_C
Cuts at: 1551 1799 1551
Size: 248 3873
KpnI G_GTAC'C
Cuts at: 1141 1141
Size: 4121
MluI A'CGCG_T
Cuts at: 3554 3554
Size: 4121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1108 2792 2976 1108
Size: 1684 184 2253
Fragments arranged by size:
2253 1684 184
MscI TGG'CCA
Cuts at: 1882 1882
Size: 4121
MslI CAynn'nnrTG
Cuts at: 2110 2237 2110
Size: 127 3994
MspA1I CmG'CkG
Cuts at: 505 770 1232 1546 1906 2919 3164 505
Size: 265 462 314 360 1013 245 1462
Fragments arranged by size:
1462 1013 462 360 314 265 245
NaeI GCC'GGC
Cuts at: 2302 2302
Size: 4121
NarI GG'CG_CC
Cuts at: 1552 1800 1552
Size: 248 3873
NcoI C'CATG_G
Cuts at: 107 654 1046 107
Size: 547 392 3182
Fragments arranged by size:
3182 547 392
NgoMIV G'CCGG_C
Cuts at: 2300 2300
Size: 4121
NspI r_CATG'y
Cuts at: 207 1174 1674 2581 207
Size: 967 500 907 1747
Fragments arranged by size:
1747 967 907 500
PciI A'CATG_T
Cuts at: 203 2577 203
Size: 2374 1747
PmeI GTTT'AAAC
Cuts at: 1120 1120
Size: 4121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 4089
PpuMI rG'GwC_Cy
Cuts at: 269 269
Size: 4121
PstI C_TGCA'G
Cuts at: 706 1168 1853 706
Size: 462 685 2974
Fragments arranged by size:
2974 685 462
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4121
PvuII CAG'CTG
Cuts at: 505 1546 1906 505
Size: 1041 360 2720
Fragments arranged by size:
2720 1041 360
RsrII CG'GwC_CG
Cuts at: 2316 2316
Size: 4121
SacI G_AGCT'C
Cuts at: 156 1135 156
Size: 979 3142
SalI G'TCGA_C
Cuts at: 1158 1158
Size: 4121
SanDI GG'GwC_CC
Cuts at: 269 269
Size: 4121
SapI GCTCTTCn'nnn_
Cuts at: 140 2144 2354 140
Size: 2004 210 1907
Fragments arranged by size:
2004 1907 210
SbfI CC_TGCA'GG
Cuts at: 1168 1168
Size: 4121
ScaI AGT'ACT
Cuts at: 3638 3638
Size: 4121
SfcI C'TryA_G
Cuts at: 33 702 824 1005 1164 1849 2842 3033 33
Size: 669 122 181 159 685 993 191 1121
Fragments arranged by size:
1121 993 685 669 191 181 159 122
SfoI GGC'GCC
Cuts at: 1553 1801 1553
Size: 248 3873
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
SmaI CCC'GGG
Cuts at: 1143 1143
Size: 4121
SmlI C'TyrA_G
Cuts at: 1276 2191 2683 2945 1276
Size: 915 492 262 2452
Fragments arranged by size:
2452 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4121
SphI G_CATG'C
Cuts at: 1174 1674 1174
Size: 500 3621
StyI C'CwwG_G
Cuts at: 107 654 1046 1255 1609 107
Size: 547 392 209 354 2619
Fragments arranged by size:
2619 547 392 354 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1011 1704 1837 2296 2505 1011
Size: 693 133 459 209 2627
Fragments arranged by size:
2627 693 459 209 133
TatI w'GTAC_w
Cuts at: 3636 3636
Size: 4121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4089
Tth111I GACn'n_nGTC
Cuts at: 1918 1918
Size: 4121
XbaI T'CTAG_A
Cuts at: 210 1152 210
Size: 942 3179
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 455 1044 1616 455
Size: 589 572 2960
Fragments arranged by size:
2960 589 572
XmaI C'CCGG_G
Cuts at: 1141 1141
Size: 4121
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI Bpu10I BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstXI Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI