Restriction map for
pF1KE1797
(Circular) (Six-base) MAPSORT of: pF1KE1797.seq Check: 3192 from: 1 to: 5642
pF1KE1797 5642 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2359 2359
Size: 5642
AccI GT'mk_AC
Cuts at: 2680 2680
Size: 5642
Acc65I G'GTAC_C
Cuts at: 2658 2658
Size: 5642
AclI AA'CG_TT
Cuts at: 5482 5482
Size: 5642
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 574 1270 2470 3467 3899 4646 4914 493
Size: 81 696 1200 997 432 747 268 1221
Fragments arranged by size:
1221 1200 997 747 696 432 268 81
AfeI AGC'GCT
Cuts at: 117 117
Size: 5642
AflIII A'CryG_T
Cuts at: 2994 4098 5075 2994
Size: 1104 977 3561
Fragments arranged by size:
3561 1104 977
AgeI A'CCGG_T
Cuts at: 4030 4030
Size: 5642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1855 1855
Size: 5642
AlwNI CAG_nnn'CTG
Cuts at: 2947 4514 2947
Size: 1567 4075
ApaI G_GGCC'C
Cuts at: 1905 1923 1905
Size: 18 5624
ApaLI G'TGCA_C
Cuts at: 1723 4412 1723
Size: 2689 2953
ApoI r'AATT_y
Cuts at: 1070 2429 2646 4995 5251 1070
Size: 1359 217 2349 256 1461
Fragments arranged by size:
2349 1461 1359 256 217
AseI AT'TA_AT
Cuts at: 20 20
Size: 5642
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5642
AvaI C'yCGr_G
Cuts at: 751 1164 1233 1606 1934 2662 751
Size: 413 69 373 328 728 3731
Fragments arranged by size:
3731 728 413 373 328 69
BamHI G'GATC_C
Cuts at: 2667 2667
Size: 5642
BanI G'GyrC_C
Cuts at: 1356 1591 2084 2111 2658 3072 3320 3355 1356
Size: 235 493 27 547 414 248 35 3643
Fragments arranged by size:
3643 547 493 414 248 235 35 27
BanII G_rGCy'C
Cuts at: 1327 1334 1905 1923 2656 3686 1327
Size: 7 571 18 733 1030 3283
Fragments arranged by size:
3283 1030 733 571 18 7
BbeI G_GCGC'C
Cuts at: 1360 1595 3076 3324 1360
Size: 235 1481 248 3678
Fragments arranged by size:
3678 1481 248 235
BbsI GAAGACnn'nnnn_
Cuts at: 1412 1412
Size: 5642
BbvCI CC'TCA_GC
Cuts at: 2222 2222
Size: 5642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2524 2558 2524
Size: 34 5608
BciVI GTATCCnnnnn_n'
Cuts at: 417 471 910 3540 4301 5642 417
Size: 54 439 2630 761 1341 417
Fragments arranged by size:
2630 1341 761 439 417 54
BclI T'GATC_A
Cuts at: 3162 3162
Size: 5642
BfrBI ATG'CAT
Cuts at: 1119 1119
Size: 5642
BglI GCCn_nnn'nGGC
Cuts at: 306 1742 306
Size: 1436 4206
BglII A'GATC_T
Cuts at: 2567 3157 5194 2567
Size: 590 2037 3015
Fragments arranged by size:
3015 2037 590
BlpI GC'TnA_GC
Cuts at: 1223 2192 2491 2754 1223
Size: 969 299 263 4111
Fragments arranged by size:
4111 969 299 263
Bme1580I G_kGCm'C
Cuts at: 1727 1905 1923 2019 2114 2136 3267 3360 4416
Size: 178 18 96 95 22 1131 93 1056
Cuts at: 4416 1727
Size: 2953
Fragments arranged by size:
2953 1131 1056 178 96 95 93 22
18
BmrI ACTGGGnnnn_n'
Cuts at: 175 2854 2935 3270 5414 175
Size: 2679 81 335 2144 403
Fragments arranged by size:
2679 2144 403 335 81
BmtI G_CTAG'C
Cuts at: 2221 2221
Size: 5642
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1248 4009 1248
Size: 2761 2881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 1090 1276 1300 1999 2048 2218 2321 2368
Size: 666 186 24 699 49 170 103 47
Cuts at: 2368 424
Size: 3698
Fragments arranged by size:
3698 699 666 186 170 103 49 47
24
Bpu10I CC'TnA_GC
Cuts at: 2222 2222
Size: 5642
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1432 2818 3697 4189 4487 1432
Size: 1386 879 492 298 2587
Fragments arranged by size:
2587 1386 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 196 5346 196
Size: 5150 492
BsaAI yAC'GTr
Cuts at: 2995 2995
Size: 5642
BsaBI GATnn'nnATC
Cuts at: 6 3156 5193 6
Size: 3150 2037 455
Fragments arranged by size:
3150 2037 455
BsaHI Gr'CG_yC
Cuts at: 1357 1592 3073 3321 4023 5519 1357
Size: 235 1481 248 702 1496 1480
Fragments arranged by size:
1496 1481 1480 702 248 235
BsaWI w'CCGG_w
Cuts at: 318 1791 2438 2831 3352 4030 4304 4451 4862
Size: 1473 647 393 521 678 274 147 411
Cuts at: 4862 318
Size: 1098
Fragments arranged by size:
1473 1098 678 647 521 411 393 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1360 1390 1360
Size: 30 5612
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 723 817 948 1297 1594 1708 2215 2578 723
Size: 94 131 349 297 114 507 363 3787
Fragments arranged by size:
3787 507 363 349 297 131 114 94
BseYI C'CCAG_C
Cuts at: 145 1804 1915 3067 3825 4402 4898 145
Size: 1659 111 1152 758 577 496 889
Fragments arranged by size:
1659 1152 889 758 577 496 111
BsiEI CG_ry'CG
Cuts at: 104 1580 3184 3230 4438 104
Size: 1476 1604 46 1208 1308
Fragments arranged by size:
1604 1476 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1334 1727 2591 2656 3434 3624 4416 1334
Size: 393 864 65 778 190 792 2560
Fragments arranged by size:
2560 864 792 778 393 190 65
BsmBI CGTCTCn'nnnn_
Cuts at: 2066 2575 2066
Size: 509 5133
Bsp1286I G_dGCh'C
Cuts at: 1327 1334 1727 1905 1923 2019 2114 2136 2591
Size: 7 393 178 18 96 95 22 455
Cuts at: 2591 2656 3267 3360 3434 3624 3686 4416 1327
Size: 65 611 93 74 190 62 730 2553
Fragments arranged by size:
2553 730 611 455 393 190 178 96
95 93 74 65 62 22 18 7
BspEI T'CCGG_A
Cuts at: 318 1791 4862 318
Size: 1473 3071 1098
Fragments arranged by size:
3071 1473 1098
BspHI T'CATG_A
Cuts at: 4818 4818
Size: 5642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2359 2692 2906 3208 3589 2359
Size: 333 214 302 381 4412
Fragments arranged by size:
4412 381 333 302 214
BsrBI CCG'CTC
Cuts at: 50 1502 1989 2057 2554 2628 3934 3988 5472
Size: 1452 487 68 497 74 1306 54 1484
Cuts at: 5472 5637 50
Size: 165 55
Fragments arranged by size:
1484 1452 1306 497 487 165 74 68
55 54
BsrDI GCAATG_nn'
Cuts at: 456 1857 3554 456
Size: 1401 1697 2544
Fragments arranged by size:
2544 1697 1401
BsrFI r'CCGG_y
Cuts at: 2081 3640 3821 4030 2081
Size: 1559 181 209 3693
Fragments arranged by size:
3693 1559 209 181
BssSI C'ACGA_G
Cuts at: 3913 4271 3913
Size: 358 5284
BstAPI GCAn_nnn'nTGC
Cuts at: 1722 1974 3019 1722
Size: 252 1045 4345
Fragments arranged by size:
4345 1045 252
BstBI TT'CG_AA
Cuts at: 4003 4003
Size: 5642
BstEII G'GTnAC_C
Cuts at: 240 240
Size: 5642
BstXI CCAn_nnnn'nTGG
Cuts at: 2187 2397 2187
Size: 210 5432
BstYI r'GATC_y
Cuts at: 1 638 1298 2567 2667 3134 3157 3492 3738
Size: 637 660 1269 100 467 23 335 246
Cuts at: 3738 4750 4836 4848 5194 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1269 1012 660 637 467 449 346 335
246 100 86 23 12
BtgI C'CryG_G
Cuts at: 172 845 878 896 1199 1574 172
Size: 673 33 18 303 375 4240
Fragments arranged by size:
4240 673 375 303 33 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1105 2173 2549 3592 3773 3936 1105
Size: 1068 376 1043 181 163 2811
Fragments arranged by size:
2811 1068 1043 376 181 163
BtsI GCAGTG_nn'
Cuts at: 1065 1131 1725 1975 2885 1065
Size: 66 594 250 910 3822
Fragments arranged by size:
3822 910 594 250 66
ClaI AT'CG_AT
Cuts at: 2633 2633
Size: 5642
DraI TTT'AAA
Cuts at: 2481 2641 2481
Size: 160 5482
DrdI GACnn_nn'nnGTC
Cuts at: 3348 4206 3348
Size: 858 4784
EaeI y'GGCC_r
Cuts at: 1140 1577 2258 2288 3227 3401 3792 3819 5164
Size: 437 681 30 939 174 391 27 1345
Cuts at: 5164 1140
Size: 1618
Fragments arranged by size:
1618 1345 939 681 437 391 174 30
27
EagI C'GGCC_G
Cuts at: 1577 3227 1577
Size: 1650 3992
EarI CTCTTCn'nnn_
Cuts at: 267 794 1307 3665 3875 267
Size: 527 513 2358 210 2034
Fragments arranged by size:
2358 2034 527 513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 291 1720 1925 3850 4160 4306 5452 291
Size: 1429 205 1925 310 146 1146 481
Fragments arranged by size:
1925 1429 1146 481 310 205 146
EcoICRI GAG'CTC
Cuts at: 1332 2654 1332
Size: 1322 4320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 493 574 1090 1270 1276 1300 1999 2048
Size: 69 81 516 180 6 24 699 49
Cuts at: 2048 2218 2321 2368 2470 3467 3899 4646 4914
Size: 170 103 47 102 997 432 747 268
Cuts at: 4914 424
Size: 1152
Fragments arranged by size:
1152 997 747 699 516 432 268 180
170 103 102 81 69 49 47 24
6
EcoNI CCTnn'n_nnAGG
Cuts at: 930 1183 2211 930
Size: 253 1028 4361
Fragments arranged by size:
4361 1028 253
EcoO109I rG'GnC_Cy
Cuts at: 923 1443 1901 1902 1919 1920 2151 2781 923
Size: 520 458 1 17 1 231 630 3784
Fragments arranged by size:
3784 630 520 458 231 17 1 1
EcoRI G'AATT_C
Cuts at: 1070 2646 1070
Size: 1576 4066
EcoRV GAT'ATC
Cuts at: 1013 1013
Size: 5642
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3661 3661
Size: 5642
FspI TGC'GCA
Cuts at: 3423 3423
Size: 5642
HaeII r_GCGC'y
Cuts at: 119 998 1290 1360 1595 3076 3324 4346 5325
Size: 879 292 70 235 1481 248 1022 979
Cuts at: 5325 119
Size: 436
Fragments arranged by size:
1481 1022 979 879 436 292 248 235
70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 572 604 628 660 924 956 1027 1059 1420
Size: 32 24 32 264 32 71 32 361
Cuts at: 1420 1452 3484 3514 3516 3546 572
Size: 32 2032 30 2 30 2668
Fragments arranged by size:
2668 2032 361 264 71 32 32 32
32 32 30 30 24 2
HincII GTy'rAC
Cuts at: 909 2681 909
Size: 1772 3870
KasI G'GCGC_C
Cuts at: 1356 1591 3072 3320 1356
Size: 235 1481 248 3678
Fragments arranged by size:
3678 1481 248 235
KpnI G_GTAC'C
Cuts at: 2662 2662
Size: 5642
MluI A'CGCG_T
Cuts at: 5075 5075
Size: 5642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 290 1241 4313 4497 290
Size: 951 3072 184 1435
Fragments arranged by size:
3072 1435 951 184
MscI TGG'CCA
Cuts at: 1142 2260 3403 1142
Size: 1118 1143 3381
Fragments arranged by size:
3381 1143 1118
MslI CAynn'nnrTG
Cuts at: 1045 1124 1670 3631 3758 1045
Size: 79 546 1961 127 2929
Fragments arranged by size:
2929 1961 546 127 79
MspA1I CmG'CkG
Cuts at: 174 552 1222 1576 1719 2108 2196 2247 2753
Size: 378 670 354 143 389 88 51 506
Cuts at: 2753 3067 3427 4440 4685 174
Size: 314 360 1013 245 1131
Fragments arranged by size:
1131 1013 670 506 389 378 360 354
314 245 143 88 51
NaeI GCC'GGC
Cuts at: 2083 3823 2083
Size: 1740 3902
NarI GG'CG_CC
Cuts at: 1357 1592 3073 3321 1357
Size: 235 1481 248 3678
Fragments arranged by size:
3678 1481 248 235
NgoMIV G'CCGG_C
Cuts at: 2081 3821 2081
Size: 1740 3902
NheI G'CTAG_C
Cuts at: 2217 2217
Size: 5642
NotI GC'GGCC_GC
Cuts at: 1577 1577
Size: 5642
NsiI A_TGCA'T
Cuts at: 1121 1121
Size: 5642
NspI r_CATG'y
Cuts at: 1648 2695 3195 4102 1648
Size: 1047 500 907 3188
Fragments arranged by size:
3188 1047 907 500
PasI CC'CwG_GG
Cuts at: 1738 2469 1738
Size: 731 4911
PciI A'CATG_T
Cuts at: 4098 4098
Size: 5642
PflMI CCAn_nnn'nTGG
Cuts at: 1278 1278
Size: 5642
PfoI T'CCnGG_A
Cuts at: 137 401 137
Size: 264 5378
PmeI GTTT'AAAC
Cuts at: 2641 2641
Size: 5642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4781 4813 4781
Size: 32 5610
PpuMI rG'GwC_Cy
Cuts at: 923 1443 2151 923
Size: 520 708 4414
Fragments arranged by size:
4414 708 520
PspOMI G'GGCC_C
Cuts at: 1901 1919 1901
Size: 18 5624
PstI C_TGCA'G
Cuts at: 1295 1928 2689 3374 1295
Size: 633 761 685 3563
Fragments arranged by size:
3563 761 685 633
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5642
PvuII CAG'CTG
Cuts at: 552 1222 2196 3067 3427 552
Size: 670 974 871 360 2767
Fragments arranged by size:
2767 974 871 670 360
RsrII CG'GwC_CG
Cuts at: 3837 3837
Size: 5642
SacI G_AGCT'C
Cuts at: 1334 2656 1334
Size: 1322 4320
SacII CC_GC'GG
Cuts at: 175 1577 175
Size: 1402 4240
SalI G'TCGA_C
Cuts at: 2679 2679
Size: 5642
SapI GCTCTTCn'nnn_
Cuts at: 3665 3875 3665
Size: 210 5432
SbfI CC_TGCA'GG
Cuts at: 2689 2689
Size: 5642
ScaI AGT'ACT
Cuts at: 5159 5159
Size: 5642
SfcI C'TryA_G
Cuts at: 33 1291 1485 1924 2685 3370 4363 4554 33
Size: 1258 194 439 761 685 993 191 1121
Fragments arranged by size:
1258 1121 993 761 685 439 194 191
SfoI GGC'GCC
Cuts at: 1358 1593 3074 3322 1358
Size: 235 1481 248 3678
Fragments arranged by size:
3678 1481 248 235
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5642
SmaI CCC'GGG
Cuts at: 1936 2664 1936
Size: 728 4914
SmlI C'TyrA_G
Cuts at: 1233 1447 2797 3712 4204 4466 1233
Size: 214 1350 915 492 262 2409
Fragments arranged by size:
2409 1350 915 492 262 214
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5642
SphI G_CATG'C
Cuts at: 2695 3195 2695
Size: 500 5142
StuI AGG'CCT
Cuts at: 2035 2206 2035
Size: 171 5471
StyI C'CwwG_G
Cuts at: 1184 1688 1784 2042 2776 3130 1184
Size: 504 96 258 734 354 3696
Fragments arranged by size:
3696 734 504 354 258 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 649 2161 2193 3225 3358 3817 4026 649
Size: 1512 32 1032 133 459 209 2265
Fragments arranged by size:
2265 1512 1032 459 209 133 32
TatI w'GTAC_w
Cuts at: 5157 5157
Size: 5642
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1360 1392 5159 5191 1360
Size: 32 3767 32 1811
Fragments arranged by size:
3767 1811 32 32
Tth111I GACn'n_nGTC
Cuts at: 345 3439 345
Size: 3094 2548
XbaI T'CTAG_A
Cuts at: 2673 2673
Size: 5642
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2328 3137 2328
Size: 809 4833
XhoI C'TCGA_G
Cuts at: 1233 1233
Size: 5642
XmaI C'CCGG_G
Cuts at: 1934 2662 1934
Size: 728 4914
XmnI GAAnn'nnTTC
Cuts at: 2429 2429
Size: 5642
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BaeI
BmgBI BsgI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I
CspCI DraIII FseI FspAI HindIII HpaI MfeI NcoI
NdeI NruI PacI PmlI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI