Restriction map for
pF1KE1742
(Circular) (Six-base) MAPSORT of: pF1KE1742.seq Check: 3710 from: 1 to: 3845
pF1KE1742 3845 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 464 464
Size: 3845
AccI GT'mk_AC
Cuts at: 883 883
Size: 3845
Acc65I G'GTAC_C
Cuts at: 861 861
Size: 3845
AclI AA'CG_TT
Cuts at: 3685 3685
Size: 3845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 787 1670 2102 2849 3117 267
Size: 520 883 432 747 268 995
Fragments arranged by size:
995 883 747 520 432 268
AfeI AGC'GCT
Cuts at: 469 469
Size: 3845
AflIII A'CryG_T
Cuts at: 1197 2301 3278 1197
Size: 1104 977 1764
Fragments arranged by size:
1764 1104 977
AgeI A'CCGG_T
Cuts at: 2233 2233
Size: 3845
AleI CACnn'nnGTG
Cuts at: 378 541 378
Size: 163 3682
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 317 559 317
Size: 242 3603
AlwNI CAG_nnn'CTG
Cuts at: 1150 2717 1150
Size: 1567 2278
ApaI G_GGCC'C
Cuts at: 310 310
Size: 3845
ApaLI G'TGCA_C
Cuts at: 2615 2615
Size: 3845
ApoI r'AATT_y
Cuts at: 849 3198 3454 849
Size: 2349 256 1240
Fragments arranged by size:
2349 1240 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3845
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
AvaI C'yCGr_G
Cuts at: 865 865
Size: 3845
BamHI G'GATC_C
Cuts at: 681 870 681
Size: 189 3656
BanI G'GyrC_C
Cuts at: 692 861 1275 1523 1558 692
Size: 169 414 248 35 2979
Fragments arranged by size:
2979 414 248 169 35
BanII G_rGCy'C
Cuts at: 281 310 859 1889 281
Size: 29 549 1030 2237
Fragments arranged by size:
2237 1030 549 29
BbeI G_GCGC'C
Cuts at: 1279 1527 1279
Size: 248 3597
BciVI GTATCCnnnnn_n'
Cuts at: 1743 2504 3845 1743
Size: 761 1341 1743
Fragments arranged by size:
1743 1341 761
BclI T'GATC_A
Cuts at: 428 1365 428
Size: 937 2908
BglII A'GATC_T
Cuts at: 1360 3397 1360
Size: 2037 1808
BlpI GC'TnA_GC
Cuts at: 957 957
Size: 3845
Bme1580I G_kGCm'C
Cuts at: 310 1470 1563 2619 310
Size: 1160 93 1056 1536
Fragments arranged by size:
1536 1160 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1057 1138 1473 3617 1057
Size: 81 335 2144 1285
Fragments arranged by size:
2144 1285 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 3845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 763
Size: 3845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1900 2392 2690 1021
Size: 879 492 298 2176
Fragments arranged by size:
2176 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 594 3549 594
Size: 2955 890
BsaAI yAC'GTr
Cuts at: 1198 1198
Size: 3845
BsaBI GATnn'nnATC
Cuts at: 6 1359 3396 6
Size: 1353 2037 455
Fragments arranged by size:
2037 1353 455
BsaHI Gr'CG_yC
Cuts at: 1276 1524 2226 3722 1276
Size: 248 702 1496 1399
Fragments arranged by size:
1496 1399 702 248
BsaWI w'CCGG_w
Cuts at: 1034 1555 2233 2507 2654 3065 1034
Size: 521 678 274 147 411 1814
Fragments arranged by size:
1814 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1270 2028 2605 3101 1270
Size: 758 577 496 2014
Fragments arranged by size:
2014 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 519 609 402
Size: 117 90 3638
Fragments arranged by size:
3638 117 90
BsiEI CG_ry'CG
Cuts at: 104 1387 1433 2641 104
Size: 1283 46 1208 1308
Fragments arranged by size:
1308 1283 1208 46
BsiHKAI G_wGCw'C
Cuts at: 281 553 859 1637 1827 2619 281
Size: 272 306 778 190 792 1507
Fragments arranged by size:
1507 792 778 306 272 190
Bsp1286I G_dGCh'C
Cuts at: 281 310 553 859 1470 1563 1637 1827 1889
Size: 29 243 306 611 93 74 190 62
Cuts at: 1889 2619 281
Size: 730 1507
Fragments arranged by size:
1507 730 611 306 243 190 93 74
62 29
BspEI T'CCGG_A
Cuts at: 3065 3065
Size: 3845
BspHI T'CATG_A
Cuts at: 3021 3021
Size: 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 176 299 464 895 1109 1411 1792 176
Size: 123 165 431 214 302 381 2229
Fragments arranged by size:
2229 431 381 302 214 165 123
BsrBI CCG'CTC
Cuts at: 50 570 747 2137 2191 3675 3840 50
Size: 520 177 1390 54 1484 165 55
Fragments arranged by size:
1484 1390 520 177 165 55 54
BsrDI GCAATG_nn'
Cuts at: 437 1757 437
Size: 1320 2525
BsrFI r'CCGG_y
Cuts at: 1843 2024 2233 1843
Size: 181 209 3455
Fragments arranged by size:
3455 209 181
BssSI C'ACGA_G
Cuts at: 2116 2474 2116
Size: 358 3487
BstAPI GCAn_nnn'nTGC
Cuts at: 1222 1222
Size: 3845
BstBI TT'CG_AA
Cuts at: 2206 2206
Size: 3845
BstEII G'GTnAC_C
Cuts at: 387 387
Size: 3845
BstXI CCAn_nnnn'nTGG
Cuts at: 797 797
Size: 3845
BstYI r'GATC_y
Cuts at: 1 681 870 1337 1360 1695 1941 2953 3039
Size: 680 189 467 23 335 246 1012 86
Cuts at: 3039 3051 3397 1
Size: 12 346 449
Fragments arranged by size:
1012 680 467 449 346 335 246 189
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3845
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1795 1976 2139 1795
Size: 181 163 3501
Fragments arranged by size:
3501 181 163
BtsI GCAGTG_nn'
Cuts at: 1088 1088
Size: 3845
DraI TTT'AAA
Cuts at: 844 844
Size: 3845
DraIII CAC_nnn'GTG
Cuts at: 504 504
Size: 3845
DrdI GACnn_nn'nnGTC
Cuts at: 1551 2409 1551
Size: 858 2987
EaeI y'GGCC_r
Cuts at: 137 1430 1604 1995 2022 3367 137
Size: 1293 174 391 27 1345 615
Fragments arranged by size:
1345 1293 615 391 174 27
EagI C'GGCC_G
Cuts at: 1430 1430
Size: 3845
EarI CTCTTCn'nnn_
Cuts at: 286 1868 2078 286
Size: 1582 210 2053
Fragments arranged by size:
2053 1582 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2053 2363 2509 3655 2053
Size: 310 146 1146 2243
Fragments arranged by size:
2243 1146 310 146
EcoICRI GAG'CTC
Cuts at: 279 857 279
Size: 578 3267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 763 787 1670 2102 2849 3117 267
Size: 496 24 883 432 747 268 995
Fragments arranged by size:
995 883 747 496 432 268 24
EcoO109I rG'GnC_Cy
Cuts at: 307 738 786 984 307
Size: 431 48 198 3168
Fragments arranged by size:
3168 431 198 48
EcoRI G'AATT_C
Cuts at: 849 849
Size: 3845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1864 1864
Size: 3845
FspI TGC'GCA
Cuts at: 1626 1626
Size: 3845
HaeII r_GCGC'y
Cuts at: 471 1279 1527 2549 3528 471
Size: 808 248 1022 979 788
Fragments arranged by size:
1022 979 808 788 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1687 1717 1719 1749 1687
Size: 30 2 30 3783
Fragments arranged by size:
3783 30 30 2
HincII GTy'rAC
Cuts at: 884 884
Size: 3845
KasI G'GCGC_C
Cuts at: 1275 1523 1275
Size: 248 3597
KpnI G_GTAC'C
Cuts at: 865 865
Size: 3845
MluI A'CGCG_T
Cuts at: 3278 3278
Size: 3845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2516 2700 2516
Size: 184 3661
MscI TGG'CCA
Cuts at: 139 1606 139
Size: 1467 2378
MslI CAynn'nnrTG
Cuts at: 378 541 821 1834 1961 378
Size: 163 280 1013 127 2262
Fragments arranged by size:
2262 1013 280 163 127
MspA1I CmG'CkG
Cuts at: 640 956 1270 1630 2643 2888 640
Size: 316 314 360 1013 245 1597
Fragments arranged by size:
1597 1013 360 316 314 245
NaeI GCC'GGC
Cuts at: 2026 2026
Size: 3845
NarI GG'CG_CC
Cuts at: 1276 1524 1276
Size: 248 3597
NcoI C'CATG_G
Cuts at: 107 107
Size: 3845
NgoMIV G'CCGG_C
Cuts at: 2024 2024
Size: 3845
NspI r_CATG'y
Cuts at: 498 634 898 1398 2305 498
Size: 136 264 500 907 2038
Fragments arranged by size:
2038 907 500 264 136
PciI A'CATG_T
Cuts at: 2301 2301
Size: 3845
PfoI T'CCnGG_A
Cuts at: 313 677 313
Size: 364 3481
PmeI GTTT'AAAC
Cuts at: 844 844
Size: 3845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 3813
PspOMI G'GGCC_C
Cuts at: 306 306
Size: 3845
PstI C_TGCA'G
Cuts at: 296 461 776 892 1577 296
Size: 165 315 116 685 2564
Fragments arranged by size:
2564 685 315 165 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3845
PvuII CAG'CTG
Cuts at: 640 1270 1630 640
Size: 630 360 2855
Fragments arranged by size:
2855 630 360
RsrII CG'GwC_CG
Cuts at: 2040 2040
Size: 3845
SacI G_AGCT'C
Cuts at: 281 859 281
Size: 578 3267
SalI G'TCGA_C
Cuts at: 882 882
Size: 3845
SapI GCTCTTCn'nnn_
Cuts at: 286 1868 2078 286
Size: 1582 210 2053
Fragments arranged by size:
2053 1582 210
SbfI CC_TGCA'GG
Cuts at: 296 892 296
Size: 596 3249
ScaI AGT'ACT
Cuts at: 3362 3362
Size: 3845
SexAI A'CCwGG_T
Cuts at: 500 500
Size: 3845
SfcI C'TryA_G
Cuts at: 33 292 457 772 888 1573 2566 2757 33
Size: 259 165 315 116 685 993 191 1121
Fragments arranged by size:
1121 993 685 315 259 191 165 116
SfoI GGC'GCC
Cuts at: 1277 1525 1277
Size: 248 3597
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
SmaI CCC'GGG
Cuts at: 867 867
Size: 3845
SmlI C'TyrA_G
Cuts at: 1000 1915 2407 2669 1000
Size: 915 492 262 2176
Fragments arranged by size:
2176 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3845
SphI G_CATG'C
Cuts at: 898 1398 898
Size: 500 3345
StyI C'CwwG_G
Cuts at: 107 979 1333 107
Size: 872 354 2619
Fragments arranged by size:
2619 872 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 368 698 1428 1561 2020 2229 368
Size: 330 730 133 459 209 1984
Fragments arranged by size:
1984 730 459 330 209 133
TatI w'GTAC_w
Cuts at: 3360 3360
Size: 3845
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 818 850 3362 3394 818
Size: 32 2512 32 1269
Fragments arranged by size:
2512 1269 32 32
Tth111I GACn'n_nGTC
Cuts at: 1642 1642
Size: 3845
XbaI T'CTAG_A
Cuts at: 876 876
Size: 3845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 508 1340 508
Size: 832 3013
XmaI C'CCGG_G
Cuts at: 865 865
Size: 3845
XmnI GAAnn'nnTTC
Cuts at: 661 661
Size: 3845
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI