Restriction map for
pF1KE1716
(Circular) (Six-base) MAPSORT of: pF1KE1716.seq Check: 8984 from: 1 to: 3773
pF1KE1716 3773 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AccI GT'mk_AC
Cuts at: 811 811
Size: 3773
Acc65I G'GTAC_C
Cuts at: 789 789
Size: 3773
AclI AA'CG_TT
Cuts at: 3613 3613
Size: 3773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 1598 2030 2777 3045 319
Size: 1279 432 747 268 1047
Fragments arranged by size:
1279 1047 747 432 268
AflIII A'CryG_T
Cuts at: 1125 2229 3206 1125
Size: 1104 977 1692
Fragments arranged by size:
1692 1104 977
AgeI A'CCGG_T
Cuts at: 2161 2161
Size: 3773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 3773
AlwNI CAG_nnn'CTG
Cuts at: 1078 2645 1078
Size: 1567 2206
ApaI G_GGCC'C
Cuts at: 710 710
Size: 3773
ApaLI G'TGCA_C
Cuts at: 2543 2543
Size: 3773
ApoI r'AATT_y
Cuts at: 408 777 3126 3382 408
Size: 369 2349 256 799
Fragments arranged by size:
2349 799 369 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
AvaI C'yCGr_G
Cuts at: 703 793 703
Size: 90 3683
BamHI G'GATC_C
Cuts at: 798 798
Size: 3773
BanI G'GyrC_C
Cuts at: 789 1203 1451 1486 789
Size: 414 248 35 3076
Fragments arranged by size:
3076 414 248 35
BanII G_rGCy'C
Cuts at: 710 787 1817 710
Size: 77 1030 2666
Fragments arranged by size:
2666 1030 77
BbeI G_GCGC'C
Cuts at: 1207 1455 1207
Size: 248 3525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3739
BciVI GTATCCnnnnn_n'
Cuts at: 470 1671 2432 3773 470
Size: 1201 761 1341 470
Fragments arranged by size:
1341 1201 761 470
BclI T'GATC_A
Cuts at: 1293 1293
Size: 3773
BglII A'GATC_T
Cuts at: 1288 3325 1288
Size: 2037 1736
BlpI GC'TnA_GC
Cuts at: 885 885
Size: 3773
Bme1580I G_kGCm'C
Cuts at: 710 1398 1491 2547 710
Size: 688 93 1056 1936
Fragments arranged by size:
1936 1056 688 93
BmrI ACTGGGnnnn_n'
Cuts at: 985 1066 1401 3545 985
Size: 81 335 2144 1213
Fragments arranged by size:
2144 1213 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 3773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 388
Size: 3773
Bpu10I CC'TnA_GC
Cuts at: 112 220 112
Size: 108 3665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 949 1828 2320 2618 234
Size: 715 879 492 298 1389
Fragments arranged by size:
1389 879 715 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3477 3477
Size: 3773
BsaAI yAC'GTr
Cuts at: 1126 1126
Size: 3773
BsaBI GATnn'nnATC
Cuts at: 6 1287 3324 6
Size: 1281 2037 455
Fragments arranged by size:
2037 1281 455
BsaHI Gr'CG_yC
Cuts at: 1204 1452 2154 3650 1204
Size: 248 702 1496 1327
Fragments arranged by size:
1496 1327 702 248
BsaWI w'CCGG_w
Cuts at: 962 1483 2161 2435 2582 2993 962
Size: 521 678 274 147 411 1742
Fragments arranged by size:
1742 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 455 660 669 455
Size: 205 9 3559
Fragments arranged by size:
3559 205 9
BseYI C'CCAG_C
Cuts at: 326 1198 1956 2533 3029 326
Size: 872 758 577 496 1070
Fragments arranged by size:
1070 872 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 652
Size: 3773
BsiEI CG_ry'CG
Cuts at: 104 1315 1361 2569 104
Size: 1211 46 1208 1308
Fragments arranged by size:
1308 1211 1208 46
BsiHKAI G_wGCw'C
Cuts at: 787 1565 1755 2547 787
Size: 778 190 792 2013
Fragments arranged by size:
2013 792 778 190
BsiWI C'GTAC_G
Cuts at: 200 200
Size: 3773
Bsp1286I G_dGCh'C
Cuts at: 710 787 1398 1491 1565 1755 1817 2547 710
Size: 77 611 93 74 190 62 730 1936
Fragments arranged by size:
1936 730 611 190 93 77 74 62
BspEI T'CCGG_A
Cuts at: 2993 2993
Size: 3773
BspHI T'CATG_A
Cuts at: 2949 2949
Size: 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 823 1037 1339 1720 823
Size: 214 302 381 2876
Fragments arranged by size:
2876 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2065 2119 3603 3768 50
Size: 2015 54 1484 165 55
Fragments arranged by size:
2015 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 387 498 1685 387
Size: 111 1187 2475
Fragments arranged by size:
2475 1187 111
BsrFI r'CCGG_y
Cuts at: 1771 1952 2161 1771
Size: 181 209 3383
Fragments arranged by size:
3383 209 181
BssSI C'ACGA_G
Cuts at: 2044 2402 2044
Size: 358 3415
BstAPI GCAn_nnn'nTGC
Cuts at: 1150 1150
Size: 3773
BstBI TT'CG_AA
Cuts at: 2134 2134
Size: 3773
BstYI r'GATC_y
Cuts at: 1 798 1265 1288 1623 1869 2881 2967 2979
Size: 797 467 23 335 246 1012 86 12
Cuts at: 2979 3325 1
Size: 346 449
Fragments arranged by size:
1012 797 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1723 1904 2067 1723
Size: 181 163 3429
Fragments arranged by size:
3429 181 163
BtsI GCAGTG_nn'
Cuts at: 1016 1016
Size: 3773
DraI TTT'AAA
Cuts at: 772 772
Size: 3773
DrdI GACnn_nn'nnGTC
Cuts at: 211 1479 2337 211
Size: 1268 858 1647
Fragments arranged by size:
1647 1268 858
EaeI y'GGCC_r
Cuts at: 1358 1532 1923 1950 3295 1358
Size: 174 391 27 1345 1836
Fragments arranged by size:
1836 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1358 1358
Size: 3773
EarI CTCTTCn'nnn_
Cuts at: 250 481 1796 2006 250
Size: 231 1315 210 2017
Fragments arranged by size:
2017 1315 231 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1981 2291 2437 3583 1981
Size: 310 146 1146 2171
Fragments arranged by size:
2171 1146 310 146
EcoICRI GAG'CTC
Cuts at: 785 785
Size: 3773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 388 1598 2030 2777 3045 319
Size: 69 1210 432 747 268 1047
Fragments arranged by size:
1210 1047 747 432 268 69
EcoO109I rG'GnC_Cy
Cuts at: 912 912
Size: 3773
EcoRI G'AATT_C
Cuts at: 777 777
Size: 3773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1792 1792
Size: 3773
FspI TGC'GCA
Cuts at: 1554 1554
Size: 3773
HaeII r_GCGC'y
Cuts at: 1207 1455 2477 3456 1207
Size: 248 1022 979 1524
Fragments arranged by size:
1524 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1615 1645 1647 1677 1615
Size: 30 2 30 3711
Fragments arranged by size:
3711 30 30 2
HincII GTy'rAC
Cuts at: 216 420 812 216
Size: 204 392 3177
Fragments arranged by size:
3177 392 204
HpaI GTT'AAC
Cuts at: 420 420
Size: 3773
KasI G'GCGC_C
Cuts at: 1203 1451 1203
Size: 248 3525
KpnI G_GTAC'C
Cuts at: 793 793
Size: 3773
MluI A'CGCG_T
Cuts at: 3206 3206
Size: 3773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2444 2628 2444
Size: 184 3589
MscI TGG'CCA
Cuts at: 1534 1534
Size: 3773
MslI CAynn'nnrTG
Cuts at: 685 1762 1889 685
Size: 1077 127 2569
Fragments arranged by size:
2569 1077 127
MspA1I CmG'CkG
Cuts at: 884 1198 1558 2571 2816 884
Size: 314 360 1013 245 1841
Fragments arranged by size:
1841 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1954 1954
Size: 3773
NarI GG'CG_CC
Cuts at: 1204 1452 1204
Size: 248 3525
NgoMIV G'CCGG_C
Cuts at: 1952 1952
Size: 3773
NspI r_CATG'y
Cuts at: 826 1326 2233 826
Size: 500 907 2366
Fragments arranged by size:
2366 907 500
PciI A'CATG_T
Cuts at: 2229 2229
Size: 3773
PfoI T'CCnGG_A
Cuts at: 546 546
Size: 3773
PmeI GTTT'AAAC
Cuts at: 772 772
Size: 3773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 3741
PspOMI G'GGCC_C
Cuts at: 706 706
Size: 3773
PstI C_TGCA'G
Cuts at: 820 1505 820
Size: 685 3088
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3773
PvuII CAG'CTG
Cuts at: 1198 1558 1198
Size: 360 3413
RsrII CG'GwC_CG
Cuts at: 1968 1968
Size: 3773
SacI G_AGCT'C
Cuts at: 787 787
Size: 3773
SalI G'TCGA_C
Cuts at: 810 810
Size: 3773
SapI GCTCTTCn'nnn_
Cuts at: 481 1796 2006 481
Size: 1315 210 2248
Fragments arranged by size:
2248 1315 210
SbfI CC_TGCA'GG
Cuts at: 820 820
Size: 3773
ScaI AGT'ACT
Cuts at: 3290 3290
Size: 3773
SfcI C'TryA_G
Cuts at: 33 603 816 1501 2494 2685 33
Size: 570 213 685 993 191 1121
Fragments arranged by size:
1121 993 685 570 213 191
SfoI GGC'GCC
Cuts at: 1205 1453 1205
Size: 248 3525
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
SmaI CCC'GGG
Cuts at: 705 795 705
Size: 90 3683
SmlI C'TyrA_G
Cuts at: 249 928 1843 2335 2597 249
Size: 679 915 492 262 1425
Fragments arranged by size:
1425 915 679 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3773
SphI G_CATG'C
Cuts at: 826 1326 826
Size: 500 3273
StyI C'CwwG_G
Cuts at: 907 1261 907
Size: 354 3419
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 558 630 1356 1489 1948 2157 558
Size: 72 726 133 459 209 2174
Fragments arranged by size:
2174 726 459 209 133 72
TatI w'GTAC_w
Cuts at: 3288 3288
Size: 3773
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 3741
Tth111I GACn'n_nGTC
Cuts at: 1570 1570
Size: 3773
XbaI T'CTAG_A
Cuts at: 804 804
Size: 3773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1268 1268
Size: 3773
XmaI C'CCGG_G
Cuts at: 703 793 703
Size: 90 3683
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BsaXI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI