Restriction map for
pF1KE1597
(Circular) (Six-base) MAPSORT of: pF1KE1597.seq Check: 6599 from: 1 to: 12353
pF1KE1597 12353 bp
With 182 enzymes: SgfI *
September 9, 2010 13:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1482 1668 4836 1482
Size: 186 3168 8999
Fragments arranged by size:
8999 3168 186
AatII G_ACGT'C
Cuts at: 743 743
Size: 12353
AccI GT'mk_AC
Cuts at: 1345 1741 2407 3604 4177 8305 9391 1345
Size: 396 666 1197 573 4128 1086 4307
Fragments arranged by size:
4307 4128 1197 1086 666 573 396
Acc65I G'GTAC_C
Cuts at: 2245 3297 9369 2245
Size: 1052 6072 5229
Fragments arranged by size:
6072 5229 1052
AclI AA'CG_TT
Cuts at: 7664 12193 7664
Size: 4529 7824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 1256 1859 2918 3356 3767 4037 4439 4871
Size: 123 603 1059 438 411 270 402 432
Cuts at: 4871 5657 5792 6080 6468 9275 10178 10610 11357
Size: 786 135 288 388 2807 903 432 747
Cuts at: 11357 11625 1133
Size: 268 1861
Fragments arranged by size:
2807 1861 1059 903 786 747 603 438
432 432 411 402 388 288 270 268
135 123
AfeI AGC'GCT
Cuts at: 8339 8339
Size: 12353
AflIII A'CryG_T
Cuts at: 1846 7230 7826 8606 9705 10809 11786 1846
Size: 5384 596 780 1099 1104 977 2413
Fragments arranged by size:
5384 2413 1104 1099 977 780 596
AgeI A'CCGG_T
Cuts at: 10741 10741
Size: 12353
AleI CACnn'nnGTG
Cuts at: 2134 3463 3586 3832 4312 4942 6703 2134
Size: 1329 123 246 480 630 1761 7784
Fragments arranged by size:
7784 1761 1329 630 480 246 123
AlwNI CAG_nnn'CTG
Cuts at: 1891 2097 2683 2749 6103 6727 7879 9658 11225
Size: 206 586 66 3354 624 1152 1779 1567
Cuts at: 11225 1891
Size: 3019
Fragments arranged by size:
3354 3019 1779 1567 1152 624 586 206
66
ApaLI G'TGCA_C
Cuts at: 8457 11123 8457
Size: 2666 9687
ApoI r'AATT_y
Cuts at: 1251 1698 2148 2365 2499 2951 3951 4025 4086
Size: 447 450 217 134 452 1000 74 61
Cuts at: 4086 5692 5939 6581 6913 6924 6933 6955 7536
Size: 1606 247 642 332 11 9 22 581
Cuts at: 7536 7721 7955 8205 8441 8662 8868 9357 11706
Size: 185 234 250 236 221 206 489 2349
Cuts at: 11706 11962 1251
Size: 256 1642
Fragments arranged by size:
2349 1642 1606 1000 642 581 489 452
450 447 332 256 250 247 236 234
221 217 206 185 134 74 61 22
11 9
AseI AT'TA_AT
Cuts at: 20 5804 20
Size: 5784 6569
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 12353
AvaI C'yCGr_G
Cuts at: 3971 9373 3971
Size: 5402 6951
AvrII C'CTAG_G
Cuts at: 585 2866 7890 585
Size: 2281 5024 5048
Fragments arranged by size:
5048 5024 2281
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2273 2306 2567 2600 5294 5327 2273
Size: 33 261 33 2694 33 9299
Fragments arranged by size:
9299 2694 261 33 33 33
BamHI G'GATC_C
Cuts at: 6887 9378 6887
Size: 2491 9862
BanI G'GyrC_C
Cuts at: 2004 2245 3297 3715 5013 5125 6088 6535 9369
Size: 241 1052 418 1298 112 963 447 2834
Cuts at: 9369 9783 10031 10066 2004
Size: 414 248 35 4291
Fragments arranged by size:
4291 2834 1298 1052 963 447 418 414
248 241 112 35
BanII G_rGCy'C
Cuts at: 8749 9367 10397 8749
Size: 618 1030 10705
Fragments arranged by size:
10705 1030 618
BbeI G_GCGC'C
Cuts at: 9787 10035 9787
Size: 248 12105
BbsI GAAGACnn'nnnn_
Cuts at: 371 743 1309 3406 4877 6725 8622 8899 371
Size: 372 566 2097 1471 1848 1897 277 3825
Fragments arranged by size:
3825 2097 1897 1848 1471 566 372 277
BbvCI CC'TCA_GC
Cuts at: 2684 3120 3185 7511 2684
Size: 436 65 4326 7526
Fragments arranged by size:
7526 4326 436 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 6791 6825 6791
Size: 34 12319
BciVI GTATCCnnnnn_n'
Cuts at: 524 2301 4335 10251 11012 12353 524
Size: 1777 2034 5916 761 1341 524
Fragments arranged by size:
5916 2034 1777 1341 761 524
BclI T'GATC_A
Cuts at: 8447 8492 9873 8447
Size: 45 1381 10927
Fragments arranged by size:
10927 1381 45
BfrBI ATG'CAT
Cuts at: 281 4737 7349 281
Size: 4456 2612 5285
Fragments arranged by size:
5285 4456 2612
BglI GCCn_nnn'nGGC
Cuts at: 2101 3706 7798 2101
Size: 1605 4092 6656
Fragments arranged by size:
6656 4092 1605
BglII A'GATC_T
Cuts at: 5645 8727 9868 11905 5645
Size: 3082 1141 2037 6093
Fragments arranged by size:
6093 3082 2037 1141
BlpI GC'TnA_GC
Cuts at: 9465 9465
Size: 12353
Bme1580I G_kGCm'C
Cuts at: 593 1389 4263 8461 9978 10071 11127 593
Size: 796 2874 4198 1517 93 1056 1819
Fragments arranged by size:
4198 2874 1819 1517 1056 796 93
BmrI ACTGGGnnnn_n'
Cuts at: 1391 1814 6346 6710 8098 9565 9646 9981 12125
Size: 423 4532 364 1388 1467 81 335 2144
Cuts at: 12125 1391
Size: 1619
Fragments arranged by size:
4532 2144 1619 1467 1388 423 364 335
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1782 4812 6447 7976 10720 1782
Size: 3030 1635 1529 2744 3415
Fragments arranged by size:
3415 3030 2744 1635 1529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 896 1526 2040 2216 5183 6417 6695 8614 896
Size: 630 514 176 2967 1234 278 1919 4635
Fragments arranged by size:
4635 2967 1919 1234 630 514 278 176
Bpu10I CC'TnA_GC
Cuts at: 1514 2684 3120 3185 3473 6620 7125 7511 1514
Size: 1170 436 65 288 3147 505 386 6356
Fragments arranged by size:
6356 3147 1170 505 436 386 288 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 583 3617 4125 9217 9529 10408 10900 11198
Size: 289 3034 508 5092 312 879 492 298
Cuts at: 11198 294
Size: 1449
Fragments arranged by size:
5092 3034 1449 879 508 492 312 298
289
BsaI GGTCTCn'nnnn_
Cuts at: 383 1645 1753 2059 2521 3475 3718 3736 4009
Size: 1262 108 306 462 954 243 18 273
Cuts at: 4009 12057 383
Size: 8048 679
Fragments arranged by size:
8048 1262 954 679 462 306 273 243
108 18
BsaAI yAC'GTr
Cuts at: 1849 9706 1849
Size: 7857 4496
BsaBI GATnn'nnATC
Cuts at: 6 9867 11904 6
Size: 9861 2037 455
Fragments arranged by size:
9861 2037 455
BsaHI Gr'CG_yC
Cuts at: 740 9223 9784 10032 10734 12230 740
Size: 8483 561 248 702 1496 863
Fragments arranged by size:
8483 1496 863 702 561 248
BsaWI w'CCGG_w
Cuts at: 8353 9542 10063 10741 11015 11162 11573 8353
Size: 1189 521 678 274 147 411 9133
Fragments arranged by size:
9133 1189 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 963 993 1125 1155 1524 1554 4614 4644 5652
Size: 30 132 30 369 30 3060 30 1008
Cuts at: 5652 5682 8375 8405 963
Size: 30 2693 30 4911
Fragments arranged by size:
4911 3060 2693 1008 369 132 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1755 3202 3655 5657 5660 8358 1755
Size: 1447 453 2002 3 2698 5750
Fragments arranged by size:
5750 2698 2002 1447 453 3
BseYI C'CCAG_C
Cuts at: 4954 5101 7554 8193 9778 10536 11113 11609 4954
Size: 147 2453 639 1585 758 577 496 5698
Fragments arranged by size:
5698 2453 1585 758 639 577 496 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 881 986 8832 9260 881
Size: 105 7846 428 3974
Fragments arranged by size:
7846 3974 428 105
BsiEI CG_ry'CG
Cuts at: 104 9895 9941 11149 104
Size: 9791 46 1208 1308
Fragments arranged by size:
9791 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1526 2983 3251 5161 6304 8461 9212 9367 10145
Size: 1457 268 1910 1143 2157 751 155 778
Cuts at: 10145 10335 11127 1526
Size: 190 792 2752
Fragments arranged by size:
2752 2157 1910 1457 1143 792 778 751
268 190 155
BsmI GAATG_Cn'
Cuts at: 2489 4425 7512 7850 2489
Size: 1936 3087 338 6992
Fragments arranged by size:
6992 3087 1936 338
Bsp1286I G_dGCh'C
Cuts at: 593 1389 1526 2983 3251 4263 5161 6304 8461
Size: 796 137 1457 268 1012 898 1143 2157
Cuts at: 8461 8749 9212 9367 9978 10071 10145 10335 10397
Size: 288 463 155 611 93 74 190 62
Cuts at: 10397 11127 593
Size: 730 1819
Fragments arranged by size:
2157 1819 1457 1143 1012 898 796 730
611 463 288 268 190 155 137 93
74 62
BspEI T'CCGG_A
Cuts at: 11573 11573
Size: 12353
BspHI T'CATG_A
Cuts at: 2166 8828 11529 2166
Size: 6662 2701 2990
Fragments arranged by size:
6662 2990 2701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1482 1668 2424 4836 9403 9617 9919 10300 1482
Size: 186 756 2412 4567 214 302 381 3535
Fragments arranged by size:
4567 3535 2412 756 381 302 214 186
BsrBI CCG'CTC
Cuts at: 50 10645 10699 12183 12348 50
Size: 10595 54 1484 165 55
Fragments arranged by size:
10595 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2067 3154 4651 5921 7722 7902 10265 2067
Size: 1087 1497 1270 1801 180 2363 4155
Fragments arranged by size:
4155 2363 1801 1497 1270 1087 180
BsrFI r'CCGG_y
Cuts at: 9155 10351 10532 10741 9155
Size: 1196 181 209 10767
Fragments arranged by size:
10767 1196 209 181
BsrGI T'GTAC_A
Cuts at: 1818 3822 4762 6404 1818
Size: 2004 940 1642 7767
Fragments arranged by size:
7767 2004 1642 940
BssSI C'ACGA_G
Cuts at: 4254 10624 10982 4254
Size: 6370 358 5625
Fragments arranged by size:
6370 5625 358
BstAPI GCAn_nnn'nTGC
Cuts at: 8980 9730 8980
Size: 750 11603
BstBI TT'CG_AA
Cuts at: 8421 10714 8421
Size: 2293 10060
BstEII G'GTnAC_C
Cuts at: 3198 3198
Size: 12353
BstXI CCAn_nnnn'nTGG
Cuts at: 874 3294 3846 3900 4287 5097 5826 8146 8379
Size: 2420 552 54 387 810 729 2320 233
Cuts at: 8379 874
Size: 4848
Fragments arranged by size:
4848 2420 2320 810 729 552 387 233
54
BstYI r'GATC_y
Cuts at: 1 2962 5645 6100 6887 7476 8727 9327 9378
Size: 2961 2683 455 787 589 1251 600 51
Cuts at: 9378 9845 9868 10203 10449 11461 11547 11559 11905
Size: 467 23 335 246 1012 86 12 346
Cuts at: 11905 1
Size: 449
Fragments arranged by size:
2961 2683 1251 1012 787 600 589 467
455 449 346 335 246 86 51 23
12
Bsu36I CC'TnA_GG
Cuts at: 4445 6422 4445
Size: 1977 10376
BtgI C'CryG_G
Cuts at: 1184 2308 3348 3380 3407 5572 5952 1184
Size: 1124 1040 32 27 2165 380 7585
Fragments arranged by size:
7585 2165 1124 1040 380 32 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 10303 10484 10647 10303
Size: 181 163 12009
Fragments arranged by size:
12009 181 163
BtsI GCAGTG_nn'
Cuts at: 781 1055 2249 2684 5129 7244 8396 8552 9100
Size: 274 1194 435 2445 2115 1152 156 548
Cuts at: 9100 9596 781
Size: 496 3538
Fragments arranged by size:
3538 2445 2115 1194 1152 548 496 435
274 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3312 3347 4842 4877 8034 8069 3312
Size: 35 1495 35 3157 35 7596
Fragments arranged by size:
7596 3157 1495 35 35 35
DraI TTT'AAA
Cuts at: 2370 7535 9352 2370
Size: 5165 1817 5371
Fragments arranged by size:
5371 5165 1817
DraIII CAC_nnn'GTG
Cuts at: 6340 7615 9069 6340
Size: 1275 1454 9624
Fragments arranged by size:
9624 1454 1275
DrdI GACnn_nn'nnGTC
Cuts at: 3069 3936 10059 10917 3069
Size: 867 6123 858 4505
Fragments arranged by size:
6123 4505 867 858
EaeI y'GGCC_r
Cuts at: 1407 3224 4335 7790 7998 9938 10112 10503 10530
Size: 1817 1111 3455 208 1940 174 391 27
Cuts at: 10530 11875 1407
Size: 1345 1885
Fragments arranged by size:
3455 1940 1885 1817 1345 1111 391 208
174 27
EagI C'GGCC_G
Cuts at: 9938 9938
Size: 12353
EarI CTCTTCn'nnn_
Cuts at: 213 517 686 4845 6872 7004 7125 7565 9144
Size: 304 169 4159 2027 132 121 440 1579
Cuts at: 9144 10376 10586 213
Size: 1232 210 1980
Fragments arranged by size:
4159 2027 1980 1579 1232 440 304 210
169 132 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7680 8916 10561 10871 11017 12163 7680
Size: 1236 1645 310 146 1146 7870
Fragments arranged by size:
7870 1645 1236 1146 310 146
EcoICRI GAG'CTC
Cuts at: 9365 9365
Size: 12353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 896 1133 1256 1526 1859 2040 2216 2918 3356
Size: 237 123 270 333 181 176 702 438
Cuts at: 3356 3767 4037 4439 4871 5183 5657 5792 6080
Size: 411 270 402 432 312 474 135 288
Cuts at: 6080 6417 6468 6695 8614 9275 10178 10610 11357
Size: 337 51 227 1919 661 903 432 747
Cuts at: 11357 11625 896
Size: 268 1624
Fragments arranged by size:
1919 1624 903 747 702 661 474 438
432 432 411 402 337 333 312 288
270 270 268 237 227 181 176 135
123 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2012 5933 7907 2012
Size: 3921 1974 6458
Fragments arranged by size:
6458 3921 1974
EcoO109I rG'GnC_Cy
Cuts at: 2017 2094 5279 7298 8989 9492 2017
Size: 77 3185 2019 1691 503 4878
Fragments arranged by size:
4878 3185 2019 1691 503 77
EcoRI G'AATT_C
Cuts at: 1251 3951 5939 6581 6933 7955 8205 8868 9357
Size: 2700 1988 642 352 1022 250 663 489
Cuts at: 9357 1251
Size: 4247
Fragments arranged by size:
4247 2700 1988 1022 663 642 489 352
250
EcoRV GAT'ATC
Cuts at: 3026 4385 6251 3026
Size: 1359 1866 9128
Fragments arranged by size:
9128 1866 1359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5541 9219 10372 5541
Size: 3678 1153 7522
Fragments arranged by size:
7522 3678 1153
FspI TGC'GCA
Cuts at: 10134 10134
Size: 12353
HaeII r_GCGC'y
Cuts at: 8341 9787 10035 11057 12036 8341
Size: 1446 248 1022 979 8658
Fragments arranged by size:
8658 1446 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 134 166 292 324 1045 1077 1321 1353 1723
Size: 32 126 32 721 32 244 32 370
Cuts at: 1723 1755 2458 2490 4374 4406 4489 4521 5097
Size: 32 703 32 1884 32 83 32 576
Cuts at: 5097 5129 5637 5652 5669 5684 6235 6267 6453
Size: 32 508 15 17 15 551 32 186
Cuts at: 6453 6485 7461 7493 7764 7796 8348 8380 8519
Size: 32 976 32 271 32 552 32 139
Cuts at: 8519 8551 9213 9245 10195 10225 10227 10257 134
Size: 32 662 32 950 30 2 30 2230
Fragments arranged by size:
2230 1884 976 950 721 703 662 576
552 551 508 370 271 244 186 139
126 83 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 17 15 15 2
HincII GTy'rAC
Cuts at: 753 774 1334 3776 5867 9221 9239 9392 753
Size: 21 560 2442 2091 3354 18 153 3714
Fragments arranged by size:
3714 3354 2442 2091 560 153 21 18
HindIII A'AGCT_T
Cuts at: 133 9300 133
Size: 9167 3186
KasI G'GCGC_C
Cuts at: 9783 10031 9783
Size: 248 12105
KpnI G_GTAC'C
Cuts at: 2249 3301 9373 2249
Size: 1052 6072 5229
Fragments arranged by size:
6072 5229 1052
MfeI C'AATT_G
Cuts at: 2555 5472 7204 2555
Size: 2917 1732 7704
Fragments arranged by size:
7704 2917 1732
MluI A'CGCG_T
Cuts at: 11786 11786
Size: 12353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3899 4577 5074 6610 8746 9153 11024 11208 3899
Size: 678 497 1536 2136 407 1871 184 5044
Fragments arranged by size:
5044 2136 1871 1536 678 497 407 184
MscI TGG'CCA
Cuts at: 1409 3226 4337 8000 10114 1409
Size: 1817 1111 3663 2114 3648
Fragments arranged by size:
3663 3648 2114 1817 1111
MslI CAynn'nnrTG
Cuts at: 459 2134 2307 2915 3463 3586 3832 4312 4942
Size: 1675 173 608 548 123 246 480 630
Cuts at: 4942 5095 5914 6570 6703 7859 8797 9055 10342
Size: 153 819 656 133 1156 938 258 1287
Cuts at: 10342 10469 459
Size: 127 2343
Fragments arranged by size:
2343 1675 1287 1156 938 819 656 630
608 548 480 258 246 173 153 133
127 123
MspA1I CmG'CkG
Cuts at: 1405 1432 1537 1675 1951 2287 2959 3109 4519
Size: 27 105 138 276 336 672 150 1410
Cuts at: 4519 5770 8542 9464 9778 10138 11151 11396 1405
Size: 1251 2772 922 314 360 1013 245 2362
Fragments arranged by size:
2772 2362 1410 1251 1013 922 672 360
336 314 276 245 150 138 105 27
NaeI GCC'GGC
Cuts at: 10534 10534
Size: 12353
NarI GG'CG_CC
Cuts at: 9784 10032 9784
Size: 248 12105
NcoI C'CATG_G
Cuts at: 1184 2308 3348 3407 5572 5952 1184
Size: 1124 1040 59 2165 380 7585
Fragments arranged by size:
7585 2165 1124 1040 380 59
NdeI CA'TA_TG
Cuts at: 231 3146 4332 231
Size: 2915 1186 8252
Fragments arranged by size:
8252 2915 1186
NgoMIV G'CCGG_C
Cuts at: 10532 10532
Size: 12353
NsiI A_TGCA'T
Cuts at: 283 4739 7351 283
Size: 4456 2612 5285
Fragments arranged by size:
5285 4456 2612
NspI r_CATG'y
Cuts at: 281 8610 9406 9906 10813 281
Size: 8329 796 500 907 1821
Fragments arranged by size:
8329 1821 907 796 500
PasI CC'CwG_GG
Cuts at: 3003 3900 7560 3003
Size: 897 3660 7796
Fragments arranged by size:
7796 3660 897
PciI A'CATG_T
Cuts at: 8606 10809 8606
Size: 2203 10150
PflMI CCAn_nnn'nTGG
Cuts at: 681 3166 681
Size: 2485 9868
PfoI T'CCnGG_A
Cuts at: 680 5020 5752 6672 680
Size: 4340 732 920 6361
Fragments arranged by size:
6361 4340 920 732
PmeI GTTT'AAAC
Cuts at: 9352 9352
Size: 12353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11492 11524 11492
Size: 32 12321
PpuMI rG'GwC_Cy
Cuts at: 2017 5279 7298 8989 2017
Size: 3262 2019 1691 5381
Fragments arranged by size:
5381 3262 2019 1691
PsiI TTA'TAA
Cuts at: 6907 8344 6907
Size: 1437 10916
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5066 5098 6309 6341 5066
Size: 32 1211 32 11078
Fragments arranged by size:
11078 1211 32 32
PstI C_TGCA'G
Cuts at: 8123 8547 9400 10085 8123
Size: 424 853 685 10391
Fragments arranged by size:
10391 853 685 424
PvuI CG_AT'CG
Cuts at: 104 104
Size: 12353
PvuII CAG'CTG
Cuts at: 1405 1432 1537 1675 1951 2287 2959 3109 4519
Size: 27 105 138 276 336 672 150 1410
Cuts at: 4519 5770 8542 9778 10138 1405
Size: 1251 2772 1236 360 3620
Fragments arranged by size:
3620 2772 1410 1251 1236 672 360 336
276 150 138 105 27
RsrII CG'GwC_CG
Cuts at: 10548 10548
Size: 12353
SacI G_AGCT'C
Cuts at: 9367 9367
Size: 12353
SalI G'TCGA_C
Cuts at: 9390 9390
Size: 12353
SanDI GG'GwC_CC
Cuts at: 8989 8989
Size: 12353
SapI GCTCTTCn'nnn_
Cuts at: 6872 7125 10376 10586 6872
Size: 253 3251 210 8639
Fragments arranged by size:
8639 3251 253 210
SbfI CC_TGCA'GG
Cuts at: 9400 9400
Size: 12353
ScaI AGT'ACT
Cuts at: 5634 11870 5634
Size: 6236 6117
SexAI A'CCwGG_T
Cuts at: 8480 8480
Size: 12353
SfcI C'TryA_G
Cuts at: 33 1427 1883 1946 2267 2669 3053 3104 4565
Size: 1394 456 63 321 402 384 51 1461
Cuts at: 4565 4997 6206 8119 8306 8543 9396 10081 11074
Size: 432 1209 1913 187 237 853 685 993
Cuts at: 11074 11265 33
Size: 191 1121
Fragments arranged by size:
1913 1461 1394 1209 1121 993 853 685
456 432 402 384 321 237 191 187
63 51
SfoI GGC'GCC
Cuts at: 9785 10033 9785
Size: 248 12105
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 12353
SmaI CCC'GGG
Cuts at: 3973 9375 3973
Size: 5402 6951
SmlI C'TyrA_G
Cuts at: 309 598 3632 4140 9232 9508 10423 10915 11177
Size: 289 3034 508 5092 276 915 492 262
Cuts at: 11177 309
Size: 1485
Fragments arranged by size:
5092 3034 1485 915 508 492 289 276
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 12353
SphI G_CATG'C
Cuts at: 281 9406 9906 281
Size: 9125 500 2728
Fragments arranged by size:
9125 2728 500
SspI AAT'ATT
Cuts at: 2191 5400 7488 2191
Size: 3209 2088 7056
Fragments arranged by size:
7056 3209 2088
StyI C'CwwG_G
Cuts at: 375 585 1184 1447 1666 2000 2308 2440 2866
Size: 210 599 263 219 334 308 132 426
Cuts at: 2866 3348 3407 5572 5952 6883 7890 9487 9841
Size: 482 59 2165 380 931 1007 1597 354
Cuts at: 9841 375
Size: 2887
Fragments arranged by size:
2887 2165 1597 1007 931 599 482 426
380 354 334 308 263 219 210 132
59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1948 2995 6135 6275 6328 8185 8287 9107 9936
Size: 1047 3140 140 53 1857 102 820 829
Cuts at: 9936 10069 10528 10737 1948
Size: 133 459 209 3564
Fragments arranged by size:
3564 3140 1857 1047 829 820 459 209
140 133 102 53
TatI w'GTAC_w
Cuts at: 653 826 1818 3822 4762 5632 6404 11868 653
Size: 173 992 2004 940 870 772 5464 1138
Fragments arranged by size:
5464 2004 1138 992 940 870 772 173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1524 1556 1800 1832 4475 4507 8375 8407 11870
Size: 32 244 32 2643 32 3868 32 3463
Cuts at: 11870 11902 1524
Size: 32 1975
Fragments arranged by size:
3868 3463 2643 1975 244 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 6465 6498 10150 6465
Size: 33 3652 8668
Fragments arranged by size:
8668 3652 33
XbaI T'CTAG_A
Cuts at: 9384 9384
Size: 12353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5094 5682 7831 8307 9848 5094
Size: 588 2149 476 1541 7599
Fragments arranged by size:
7599 2149 1541 588 476
XmaI C'CCGG_G
Cuts at: 3971 9373 3971
Size: 5402 6951
XmnI GAAnn'nnTTC
Cuts at: 1199 9004 1199
Size: 7805 4548
ZraI GAC'GTC
Cuts at: 741 741
Size: 12353
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI ApaI AscI BmgBI BmtI
BsiWI BsmBI BssHII BstZ17I ClaI FseI FspAI HpaI
NheI NotI NruI PacI PmlI PshAI PspOMI PspXI
SacII SfiI SgrAI SnaBI SrfI StuI SwaI XhoI