Restriction map for
pF1KE1590
(Circular) (Six-base) MAPSORT of: pF1KE1590.seq Check: 833 from: 1 to: 6617
pF1KE1590 6617 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3402 3402
Size: 6617
AccI GT'mk_AC
Cuts at: 3655 3655
Size: 6617
Acc65I G'GTAC_C
Cuts at: 3633 3633
Size: 6617
AclI AA'CG_TT
Cuts at: 6457 6457
Size: 6617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 873 1022 1860 2395 3067 3502 4442 4874 5621
Size: 149 838 535 672 435 940 432 747
Cuts at: 5621 5889 873
Size: 268 1601
Fragments arranged by size:
1601 940 838 747 672 535 435 432
268 149
AflIII A'CryG_T
Cuts at: 608 1089 1157 3969 5073 6050 608
Size: 481 68 2812 1104 977 1175
Fragments arranged by size:
2812 1175 1104 977 481 68
AgeI A'CCGG_T
Cuts at: 5005 5005
Size: 6617
AleI CACnn'nnGTG
Cuts at: 2525 2525
Size: 6617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 817 817
Size: 6617
AlwNI CAG_nnn'CTG
Cuts at: 2304 3922 5489 2304
Size: 1618 1567 3432
Fragments arranged by size:
3432 1618 1567
ApaI G_GGCC'C
Cuts at: 2222 2222
Size: 6617
ApaLI G'TGCA_C
Cuts at: 1167 3585 5387 1167
Size: 2418 1802 2397
Fragments arranged by size:
2418 2397 1802
ApoI r'AATT_y
Cuts at: 1050 1323 2539 3621 5970 6226 1050
Size: 273 1216 1082 2349 256 1441
Fragments arranged by size:
2349 1441 1216 1082 273 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6617
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6617
AvaI C'yCGr_G
Cuts at: 1646 1940 1950 2928 3595 3637 1646
Size: 294 10 978 667 42 4626
Fragments arranged by size:
4626 978 667 294 42 10
BamHI G'GATC_C
Cuts at: 363 3428 3642 363
Size: 3065 214 3338
Fragments arranged by size:
3338 3065 214
BanI G'GyrC_C
Cuts at: 1251 3633 4047 4295 4330 1251
Size: 2382 414 248 35 3538
Fragments arranged by size:
3538 2382 414 248 35
BanII G_rGCy'C
Cuts at: 190 742 1783 2222 2267 3566 3602 3631 4661
Size: 552 1041 439 45 1299 36 29 1030
Cuts at: 4661 190
Size: 2146
Fragments arranged by size:
2146 1299 1041 1030 552 439 45 36
29
BbeI G_GCGC'C
Cuts at: 1255 4051 4299 1255
Size: 2796 248 3573
Fragments arranged by size:
3573 2796 248
BbsI GAAGACnn'nnnn_
Cuts at: 258 473 1115 2077 258
Size: 215 642 962 4798
Fragments arranged by size:
4798 962 642 215
BbvCI CC'TCA_GC
Cuts at: 242 401 242
Size: 159 6458
BciVI GTATCCnnnnn_n'
Cuts at: 4515 5276 6617 4515
Size: 761 1341 4515
Fragments arranged by size:
4515 1341 761
BclI T'GATC_A
Cuts at: 513 4137 513
Size: 3624 2993
BglII A'GATC_T
Cuts at: 1612 4132 6169 1612
Size: 2520 2037 2060
Fragments arranged by size:
2520 2060 2037
BlpI GC'TnA_GC
Cuts at: 2440 2549 3729 2440
Size: 109 1180 5328
Fragments arranged by size:
5328 1180 109
Bme1580I G_kGCm'C
Cuts at: 1171 2222 3589 4242 4335 5391 1171
Size: 1051 1367 653 93 1056 2397
Fragments arranged by size:
2397 1367 1056 1051 653 93
BmgBI CAC'GTC
Cuts at: 714 2515 3294 714
Size: 1801 779 4037
Fragments arranged by size:
4037 1801 779
BmrI ACTGGGnnnn_n'
Cuts at: 1559 3829 3910 4245 6389 1559
Size: 2270 81 335 2144 1787
Fragments arranged by size:
2270 2144 1787 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4984 4984
Size: 6617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2109 2432 2992 3529 2109
Size: 323 560 537 5197
Fragments arranged by size:
5197 560 537 323
Bpu10I CC'TnA_GC
Cuts at: 242 338 401 1247 3463 242
Size: 96 63 846 2216 3396
Fragments arranged by size:
3396 2216 846 96 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2830 3460 3793 4672 5164 5462 2830
Size: 630 333 879 492 298 3985
Fragments arranged by size:
3985 879 630 492 333 298
BsaI GGTCTCn'nnnn_
Cuts at: 162 1410 2788 2948 6321 162
Size: 1248 1378 160 3373 458
Fragments arranged by size:
3373 1378 1248 458 160
BsaAI yAC'GTr
Cuts at: 1092 1158 3970 1092
Size: 66 2812 3739
Fragments arranged by size:
3739 2812 66
BsaBI GATnn'nnATC
Cuts at: 6 4131 6168 6
Size: 4125 2037 455
Fragments arranged by size:
4125 2037 455
BsaHI Gr'CG_yC
Cuts at: 1252 2002 4048 4296 4998 6494 1252
Size: 750 2046 248 702 1496 1375
Fragments arranged by size:
2046 1496 1375 750 702 248
BsaWI w'CCGG_w
Cuts at: 1126 3806 4327 5005 5279 5426 5837 1126
Size: 2680 521 678 274 147 411 1906
Fragments arranged by size:
2680 1906 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1798 1828 3568 3598 1798
Size: 30 1740 30 4817
Fragments arranged by size:
4817 1740 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 592 916 1783 1791 2989 3314 3553 592
Size: 324 867 8 1198 325 239 3656
Fragments arranged by size:
3656 1198 867 325 324 239 8
BseYI C'CCAG_C
Cuts at: 818 1259 1865 2169 2578 2636 2699 2992 3045
Size: 441 606 304 409 58 63 293 53
Cuts at: 3045 3565 4042 4800 5377 5873 818
Size: 520 477 758 577 496 1562
Fragments arranged by size:
1562 758 606 577 520 496 477 441
409 304 293 63 58 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 967 1255 1276 568
Size: 399 288 21 5909
Fragments arranged by size:
5909 399 288 21
BsiEI CG_ry'CG
Cuts at: 104 645 3517 3573 4159 4205 5413 104
Size: 541 2872 56 586 46 1208 1308
Fragments arranged by size:
2872 1308 1208 586 541 56 46
BsiHKAI G_wGCw'C
Cuts at: 635 1171 1783 1996 2267 2981 3589 3631 4409
Size: 536 612 213 271 714 608 42 778
Cuts at: 4409 4599 5391 635
Size: 190 792 1861
Fragments arranged by size:
1861 792 778 714 612 608 536 271
213 190 42
BsmI GAATG_Cn'
Cuts at: 201 1921 2082 201
Size: 1720 161 4736
Fragments arranged by size:
4736 1720 161
BsmBI CGTCTCn'nnnn_
Cuts at: 1993 1993
Size: 6617
Bsp1286I G_dGCh'C
Cuts at: 190 635 742 1171 1783 1996 2222 2267 2981
Size: 445 107 429 612 213 226 45 714
Cuts at: 2981 3566 3589 3602 3631 4242 4335 4409 4599
Size: 585 23 13 29 611 93 74 190
Cuts at: 4599 4661 5391 190
Size: 62 730 1416
Fragments arranged by size:
1416 730 714 612 611 585 445 429
226 213 190 107 93 74 62 45
29 23 13
BspEI T'CCGG_A
Cuts at: 1126 5837 1126
Size: 4711 1906
BspHI T'CATG_A
Cuts at: 432 2007 2940 5793 432
Size: 1575 933 2853 1256
Fragments arranged by size:
2853 1575 1256 933
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1944 3402 3667 3881 4183 4564 1944
Size: 1458 265 214 302 381 3997
Fragments arranged by size:
3997 1458 381 302 265 214
BsrBI CCG'CTC
Cuts at: 50 3513 4909 4963 6447 6612 50
Size: 3463 1396 54 1484 165 55
Fragments arranged by size:
3463 1484 1396 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4529 4529
Size: 6617
BsrFI r'CCGG_y
Cuts at: 479 675 4615 4796 5005 479
Size: 196 3940 181 209 2091
Fragments arranged by size:
3940 2091 209 196 181
BssSI C'ACGA_G
Cuts at: 2258 2980 4888 5246 2258
Size: 722 1908 358 3629
Fragments arranged by size:
3629 1908 722 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1290 2508 3492 3994 1290
Size: 1218 984 502 3913
Fragments arranged by size:
3913 1218 984 502
BstBI TT'CG_AA
Cuts at: 4978 4978
Size: 6617
BstXI CCAn_nnnn'nTGG
Cuts at: 787 1689 787
Size: 902 5715
BstYI r'GATC_y
Cuts at: 1 363 1612 1692 2909 3428 3642 4109 4132
Size: 362 1249 80 1217 519 214 467 23
Cuts at: 4132 4467 4713 5725 5811 5823 6169 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1249 1217 1012 519 467 449 362 346
335 246 214 86 80 23 12
BtgI C'CryG_G
Cuts at: 107 3602 107
Size: 3495 3122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1508 3240 4567 4748 4911 1508
Size: 1732 1327 181 163 3214
Fragments arranged by size:
3214 1732 1327 181 163
BtsI GCAGTG_nn'
Cuts at: 585 1238 3474 3860 585
Size: 653 2236 386 3342
Fragments arranged by size:
3342 2236 653 386
ClaI AT'CG_AT
Cuts at: 3314 3314
Size: 6617
DraI TTT'AAA
Cuts at: 2838 3105 3616 2838
Size: 267 511 5839
Fragments arranged by size:
5839 511 267
DraIII CAC_nnn'GTG
Cuts at: 1567 1989 1567
Size: 422 6195
DrdI GACnn_nn'nnGTC
Cuts at: 4323 5181 4323
Size: 858 5759
EaeI y'GGCC_r
Cuts at: 642 993 1226 2887 4202 4376 4767 4794 6139
Size: 351 233 1661 1315 174 391 27 1345
Cuts at: 6139 642
Size: 1120
Fragments arranged by size:
1661 1345 1315 1120 391 351 233 174
27
EagI C'GGCC_G
Cuts at: 642 4202 642
Size: 3560 3057
EarI CTCTTCn'nnn_
Cuts at: 640 988 1394 1472 1838 2822 4640 4850 640
Size: 348 406 78 366 984 1818 210 2407
Fragments arranged by size:
2407 1818 984 406 366 348 210 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 662 3567 4825 5135 5281 6427 662
Size: 2905 1258 310 146 1146 852
Fragments arranged by size:
2905 1258 1146 852 310 146
EcoICRI GAG'CTC
Cuts at: 1781 2265 3629 1781
Size: 484 1364 4769
Fragments arranged by size:
4769 1364 484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 873 1022 1860 2109 2395 2432 2992 3067 3502
Size: 149 838 249 286 37 560 75 435
Cuts at: 3502 3529 4442 4874 5621 5889 873
Size: 27 913 432 747 268 1601
Fragments arranged by size:
1601 913 838 747 560 435 432 286
268 249 149 75 37 27
EcoNI CCTnn'n_nnAGG
Cuts at: 341 1210 1878 341
Size: 869 668 5080
Fragments arranged by size:
5080 869 668
EcoO109I rG'GnC_Cy
Cuts at: 867 1809 1946 2219 3756 867
Size: 942 137 273 1537 3728
Fragments arranged by size:
3728 1537 942 273 137
EcoRI G'AATT_C
Cuts at: 1323 3621 1323
Size: 2298 4319
EcoRV GAT'ATC
Cuts at: 960 1064 960
Size: 104 6513
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 319 1779 3325 4636 319
Size: 1460 1546 1311 2300
Fragments arranged by size:
2300 1546 1460 1311
FspI TGC'GCA
Cuts at: 805 4398 805
Size: 3593 3024
HaeII r_GCGC'y
Cuts at: 1255 2373 4051 4299 5321 6300 1255
Size: 1118 1678 248 1022 979 1572
Fragments arranged by size:
1678 1572 1118 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1798 1830 2011 2043 2516 2548 3219 3251 4459
Size: 32 181 32 473 32 671 32 1208
Cuts at: 4459 4489 4491 4521 1798
Size: 30 2 30 3894
Fragments arranged by size:
3894 1208 671 473 181 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 355 3177 3656 355
Size: 2822 479 3316
Fragments arranged by size:
3316 2822 479
HindIII A'AGCT_T
Cuts at: 3272 3272
Size: 6617
KasI G'GCGC_C
Cuts at: 1251 4047 4295 1251
Size: 2796 248 3573
Fragments arranged by size:
3573 2796 248
KpnI G_GTAC'C
Cuts at: 3637 3637
Size: 6617
MluI A'CGCG_T
Cuts at: 6050 6050
Size: 6617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5288 5472 5288
Size: 184 6433
MscI TGG'CCA
Cuts at: 995 4378 995
Size: 3383 3234
MslI CAynn'nnrTG
Cuts at: 220 1687 1746 2045 2525 2819 4606 4733 220
Size: 1467 59 299 480 294 1787 127 2104
Fragments arranged by size:
2104 1787 1467 480 299 294 127 59
MspA1I CmG'CkG
Cuts at: 180 719 881 1263 1857 2628 3045 3728 4042
Size: 539 162 382 594 771 417 683 314
Cuts at: 4042 4402 5415 5660 180
Size: 360 1013 245 1137
Fragments arranged by size:
1137 1013 771 683 594 539 417 382
360 314 245 162
NaeI GCC'GGC
Cuts at: 677 4798 677
Size: 4121 2496
NarI GG'CG_CC
Cuts at: 1252 4048 4296 1252
Size: 2796 248 3573
Fragments arranged by size:
3573 2796 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6617
NdeI CA'TA_TG
Cuts at: 2816 2816
Size: 6617
NgoMIV G'CCGG_C
Cuts at: 675 4796 675
Size: 4121 2496
NotI GC'GGCC_GC
Cuts at: 642 642
Size: 6617
NspI r_CATG'y
Cuts at: 2647 3670 4170 5077 2647
Size: 1023 500 907 4187
Fragments arranged by size:
4187 1023 907 500
PasI CC'CwG_GG
Cuts at: 230 230
Size: 6617
PciI A'CATG_T
Cuts at: 5073 5073
Size: 6617
PflMI CCAn_nnn'nTGG
Cuts at: 2766 3345 2766
Size: 579 6038
PfoI T'CCnGG_A
Cuts at: 2086 2448 2086
Size: 362 6255
PmeI GTTT'AAAC
Cuts at: 3616 3616
Size: 6617
PmlI CAC'GTG
Cuts at: 1092 1092
Size: 6617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5756 5788 5756
Size: 32 6585
PpuMI rG'GwC_Cy
Cuts at: 1809 1946 1809
Size: 137 6480
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 6617
PspOMI G'GGCC_C
Cuts at: 2218 2218
Size: 6617
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2661 2693 2661
Size: 32 6585
PstI C_TGCA'G
Cuts at: 179 860 1673 3152 3530 3664 4349 179
Size: 681 813 1479 378 134 685 2447
Fragments arranged by size:
2447 1479 813 685 681 378 134
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6617
PvuII CAG'CTG
Cuts at: 180 1263 2628 4042 4402 180
Size: 1083 1365 1414 360 2395
Fragments arranged by size:
2395 1414 1365 1083 360
RsrII CG'GwC_CG
Cuts at: 4812 4812
Size: 6617
SacI G_AGCT'C
Cuts at: 1783 2267 3631 1783
Size: 484 1364 4769
Fragments arranged by size:
4769 1364 484
SalI G'TCGA_C
Cuts at: 3654 3654
Size: 6617
SanDI GG'GwC_CC
Cuts at: 1946 1946
Size: 6617
SapI GCTCTTCn'nnn_
Cuts at: 640 988 1838 4640 4850 640
Size: 348 850 2802 210 2407
Fragments arranged by size:
2802 2407 850 348 210
SbfI CC_TGCA'GG
Cuts at: 1673 3664 1673
Size: 1991 4626
ScaI AGT'ACT
Cuts at: 6134 6134
Size: 6617
SfcI C'TryA_G
Cuts at: 33 175 856 1669 2420 3148 3526 3660 4345
Size: 142 681 813 751 728 378 134 685
Cuts at: 4345 5338 5529 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 813 751 728 685 681 378
191 142 134
SfoI GGC'GCC
Cuts at: 1253 4049 4297 1253
Size: 2796 248 3573
Fragments arranged by size:
3573 2796 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6617
SmaI CCC'GGG
Cuts at: 1648 1942 1952 3639 1648
Size: 294 10 1687 4626
Fragments arranged by size:
4626 1687 294 10
SmlI C'TyrA_G
Cuts at: 2845 2928 3439 3772 4687 5179 5441 2845
Size: 83 511 333 915 492 262 4021
Fragments arranged by size:
4021 915 511 492 333 262 83
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6617
SphI G_CATG'C
Cuts at: 2647 3670 4170 2647
Size: 1023 500 5094
Fragments arranged by size:
5094 1023 500
SspI AAT'ATT
Cuts at: 942 1545 942
Size: 603 6014
StuI AGG'CCT
Cuts at: 1216 1667 1762 1873 2743 1216
Size: 451 95 111 870 5090
Fragments arranged by size:
5090 870 451 111 95
StyI C'CwwG_G
Cuts at: 107 2661 2778 3204 3751 4105 107
Size: 2554 117 426 547 354 2619
Fragments arranged by size:
2619 2554 547 426 354 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1434 1578 2699 4200 4333 4792 5001 1434
Size: 144 1121 1501 133 459 209 3050
Fragments arranged by size:
3050 1501 1121 459 209 144 133
TatI w'GTAC_w
Cuts at: 1714 6132 1714
Size: 4418 2199
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 577 609 2244 2276 3568 3600 6134 6166 577
Size: 32 1635 32 1292 32 2534 32 1028
Fragments arranged by size:
2534 1635 1292 1028 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 472 4414 472
Size: 3942 2675
XbaI T'CTAG_A
Cuts at: 2754 3648 2754
Size: 894 5723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3207 4112 3207
Size: 905 5712
XhoI C'TCGA_G
Cuts at: 2928 2928
Size: 6617
XmaI C'CCGG_G
Cuts at: 1646 1940 1950 3637 1646
Size: 294 10 1687 4626
Fragments arranged by size:
4626 1687 294 10
XmnI GAAnn'nnTTC
Cuts at: 1323 1403 1323
Size: 80 6537
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BcgI BfrBI BglI BmtI BsiWI BsrGI BssHII BstEII
BstZ17I Bsu36I CspCI FseI FspAI HpaI MfeI NheI
NruI NsiI PacI PshAI PspXI SacII SexAI SfiI
SgrAI SnaBI SrfI SwaI ZraI