Restriction map for
pF1KE1565
(Circular) (Six-base) MAPSORT of: pF1KE1565.seq Check: 5922 from: 1 to: 5309
pF1KE1565 5309 bp
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AccI GT'mk_AC
Cuts at: 2347 2347
Size: 5309
Acc65I G'GTAC_C
Cuts at: 2325 2325
Size: 5309
AclI AA'CG_TT
Cuts at: 1661 5149 1661
Size: 3488 1821
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 357 390 3134 3566 4313 4581 187
Size: 170 33 2744 432 747 268 915
Fragments arranged by size:
2744 915 747 432 268 170 33
AflIII A'CryG_T
Cuts at: 647 2256 2661 3765 4742 647
Size: 1609 405 1104 977 1214
Fragments arranged by size:
1609 1214 1104 977 405
AgeI A'CCGG_T
Cuts at: 3697 3697
Size: 5309
AleI CACnn'nnGTG
Cuts at: 1459 1459
Size: 5309
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1177 1209 1177
Size: 32 5277
AlwNI CAG_nnn'CTG
Cuts at: 782 1905 2614 4181 782
Size: 1123 709 1567 1910
Fragments arranged by size:
1910 1567 1123 709
ApaLI G'TGCA_C
Cuts at: 4079 4079
Size: 5309
ApoI r'AATT_y
Cuts at: 577 1191 1555 1810 2313 4662 4918 577
Size: 614 364 255 503 2349 256 968
Fragments arranged by size:
2349 968 614 503 364 256 255
AseI AT'TA_AT
Cuts at: 20 1498 20
Size: 1478 3831
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5309
AvaI C'yCGr_G
Cuts at: 218 2329 218
Size: 2111 3198
BamHI G'GATC_C
Cuts at: 940 2334 940
Size: 1394 3915
BanI G'GyrC_C
Cuts at: 213 2325 2739 2987 3022 213
Size: 2112 414 248 35 2500
Fragments arranged by size:
2500 2112 414 248 35
BanII G_rGCy'C
Cuts at: 1024 1847 2323 3353 1024
Size: 823 476 1030 2980
Fragments arranged by size:
2980 1030 823 476
BbeI G_GCGC'C
Cuts at: 2743 2991 2743
Size: 248 5061
BbsI GAAGACnn'nnnn_
Cuts at: 545 545
Size: 5309
BciVI GTATCCnnnnn_n'
Cuts at: 3207 3968 5309 3207
Size: 761 1341 3207
Fragments arranged by size:
3207 1341 761
BclI T'GATC_A
Cuts at: 2829 2829
Size: 5309
BglII A'GATC_T
Cuts at: 2824 4861 2824
Size: 2037 3272
BlpI GC'TnA_GC
Cuts at: 2421 2421
Size: 5309
Bme1580I G_kGCm'C
Cuts at: 218 2934 3027 4083 218
Size: 2716 93 1056 1444
Fragments arranged by size:
2716 1444 1056 93
BmgBI CAC'GTC
Cuts at: 1833 2277 1833
Size: 444 4865
BmrI ACTGGGnnnn_n'
Cuts at: 457 2521 2602 2937 5081 457
Size: 2064 81 335 2144 685
Fragments arranged by size:
2144 2064 685 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3676 3676
Size: 5309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 1861 2141 804
Size: 1057 280 3972
Fragments arranged by size:
3972 1057 280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2485 3364 3856 4154 2485
Size: 879 492 298 3640
Fragments arranged by size:
3640 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1945 2211 5013 1945
Size: 266 2802 2241
Fragments arranged by size:
2802 2241 266
BsaAI yAC'GTr
Cuts at: 650 2662 650
Size: 2012 3297
BsaBI GATnn'nnATC
Cuts at: 6 2008 2823 4860 6
Size: 2002 815 2037 455
Fragments arranged by size:
2037 2002 815 455
BsaHI Gr'CG_yC
Cuts at: 224 968 2740 2988 3690 5186 224
Size: 744 1772 248 702 1496 347
Fragments arranged by size:
1772 1496 744 702 347 248
BsaWI w'CCGG_w
Cuts at: 1016 1037 1727 2498 3019 3697 3971 4118 4529
Size: 21 690 771 521 678 274 147 411
Cuts at: 4529 1016
Size: 1796
Fragments arranged by size:
1796 771 690 678 521 411 274 147
21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 777 807 1834 1864 777
Size: 30 1027 30 4222
Fragments arranged by size:
4222 1027 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1535 1535
Size: 5309
BseYI C'CCAG_C
Cuts at: 1052 1695 2734 3492 4069 4565 1052
Size: 643 1039 758 577 496 1796
Fragments arranged by size:
1796 1039 758 643 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1906 2197 2299 382
Size: 1524 291 102 3392
Fragments arranged by size:
3392 1524 291 102
BsiEI CG_ry'CG
Cuts at: 104 2851 2897 4105 104
Size: 2747 46 1208 1308
Fragments arranged by size:
2747 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 846 1024 2323 3101 3291 4083 846
Size: 178 1299 778 190 792 2072
Fragments arranged by size:
2072 1299 792 778 190 178
BsmI GAATG_Cn'
Cuts at: 915 915
Size: 5309
BsmBI CGTCTCn'nnnn_
Cuts at: 1816 1816
Size: 5309
Bsp1286I G_dGCh'C
Cuts at: 218 846 1024 1847 2323 2934 3027 3101 3291
Size: 628 178 823 476 611 93 74 190
Cuts at: 3291 3353 4083 218
Size: 62 730 1444
Fragments arranged by size:
1444 823 730 628 611 476 190 178
93 74 62
BspEI T'CCGG_A
Cuts at: 1727 4529 1727
Size: 2802 2507
BspHI T'CATG_A
Cuts at: 515 1643 4485 515
Size: 1128 2842 1339
Fragments arranged by size:
2842 1339 1128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 194 239 2359 2573 2875 3256 194
Size: 45 2120 214 302 381 2247
Fragments arranged by size:
2247 2120 381 302 214 45
BsrBI CCG'CTC
Cuts at: 50 3601 3655 5139 5304 50
Size: 3551 54 1484 165 55
Fragments arranged by size:
3551 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 908 3221 908
Size: 2313 2996
BsrFI r'CCGG_y
Cuts at: 1882 2195 3307 3488 3697 1882
Size: 313 1112 181 209 3494
Fragments arranged by size:
3494 1112 313 209 181
BssSI C'ACGA_G
Cuts at: 252 3580 3938 252
Size: 3328 358 1623
Fragments arranged by size:
3328 1623 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2686 2686
Size: 5309
BstBI TT'CG_AA
Cuts at: 621 3670 621
Size: 3049 2260
BstXI CCAn_nnnn'nTGG
Cuts at: 983 983
Size: 5309
BstYI r'GATC_y
Cuts at: 1 399 940 2334 2801 2824 3159 3405 4417
Size: 398 541 1394 467 23 335 246 1012
Cuts at: 4417 4503 4515 4861 1
Size: 86 12 346 449
Fragments arranged by size:
1394 1012 541 467 449 398 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 2019 2300 2019
Size: 281 5028
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1334 3259 3440 3603 1334
Size: 1925 181 163 3040
Fragments arranged by size:
3040 1925 181 163
BtsI GCAGTG_nn'
Cuts at: 1227 2185 2247 2552 1227
Size: 958 62 305 3984
Fragments arranged by size:
3984 958 305 62
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1470 1505 2001 2036 1470
Size: 35 496 35 4743
Fragments arranged by size:
4743 496 35 35
DraI TTT'AAA
Cuts at: 2078 2308 2078
Size: 230 5079
DrdI GACnn_nn'nnGTC
Cuts at: 928 3015 3873 928
Size: 2087 858 2364
Fragments arranged by size:
2364 2087 858
EaeI y'GGCC_r
Cuts at: 1044 1368 1967 2894 3068 3459 3486 4831 1044
Size: 324 599 927 174 391 27 1345 1522
Fragments arranged by size:
1522 1345 927 599 391 324 174 27
EagI C'GGCC_G
Cuts at: 2894 2894
Size: 5309
EarI CTCTTCn'nnn_
Cuts at: 138 409 664 710 858 1571 3332 3542 138
Size: 271 255 46 148 713 1761 210 1905
Fragments arranged by size:
1905 1761 713 271 255 210 148 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3517 3827 3973 5119 3517
Size: 310 146 1146 3707
Fragments arranged by size:
3707 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1022 2321 1022
Size: 1299 4010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 357 390 804 1861 2141 3134 3566 4313
Size: 170 33 414 1057 280 993 432 747
Cuts at: 4313 4581 187
Size: 268 915
Fragments arranged by size:
1057 993 915 747 432 414 280 268
170 33
EcoNI CCTnn'n_nnAGG
Cuts at: 268 268
Size: 5309
EcoO109I rG'GnC_Cy
Cuts at: 1797 2448 1797
Size: 651 4658
EcoRI G'AATT_C
Cuts at: 1191 1810 2313 1191
Size: 619 503 4187
Fragments arranged by size:
4187 619 503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 259 2312 3328 259
Size: 2053 1016 2240
Fragments arranged by size:
2240 2053 1016
FspI TGC'GCA
Cuts at: 3090 3090
Size: 5309
HaeII r_GCGC'y
Cuts at: 2743 2991 4013 4992 2743
Size: 248 1022 979 3060
Fragments arranged by size:
3060 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 514 546 777 809 1834 1866 3151 3181 3183
Size: 32 231 32 1025 32 1285 30 2
Cuts at: 3183 3213 514
Size: 30 2610
Fragments arranged by size:
2610 1285 1025 231 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 228 999 1416 1665 2348 228
Size: 771 417 249 683 3189
Fragments arranged by size:
3189 771 683 417 249
KasI G'GCGC_C
Cuts at: 2739 2987 2739
Size: 248 5061
KpnI G_GTAC'C
Cuts at: 2329 2329
Size: 5309
MluI A'CGCG_T
Cuts at: 4742 4742
Size: 5309
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 918 1251 1682 3980 4164 918
Size: 333 431 2298 184 2063
Fragments arranged by size:
2298 2063 431 333 184
MscI TGG'CCA
Cuts at: 1046 1969 3070 1046
Size: 923 1101 3285
Fragments arranged by size:
3285 1101 923
MslI CAynn'nnrTG
Cuts at: 463 1139 1370 1459 2050 2111 2261 3298 3425
Size: 676 231 89 591 61 150 1037 127
Cuts at: 3425 463
Size: 2347
Fragments arranged by size:
2347 1037 676 591 231 150 127 89
61
MspA1I CmG'CkG
Cuts at: 175 1902 2420 2734 3094 4107 4352 175
Size: 1727 518 314 360 1013 245 1132
Fragments arranged by size:
1727 1132 1013 518 360 314 245
NaeI GCC'GGC
Cuts at: 1884 2197 3490 1884
Size: 313 1293 3703
Fragments arranged by size:
3703 1293 313
NarI GG'CG_CC
Cuts at: 2740 2988 2740
Size: 248 5061
NcoI C'CATG_G
Cuts at: 2300 2300
Size: 5309
NdeI CA'TA_TG
Cuts at: 384 384
Size: 5309
NgoMIV G'CCGG_C
Cuts at: 1882 2195 3488 1882
Size: 313 1293 3703
Fragments arranged by size:
3703 1293 313
NspI r_CATG'y
Cuts at: 588 1329 2260 2362 2862 3769 588
Size: 741 931 102 500 907 2128
Fragments arranged by size:
2128 931 907 741 500 102
PasI CC'CwG_GG
Cuts at: 2201 2201
Size: 5309
PciI A'CATG_T
Cuts at: 2256 3765 2256
Size: 1509 3800
PflMI CCAn_nnn'nTGG
Cuts at: 782 782
Size: 5309
PfoI T'CCnGG_A
Cuts at: 121 812 1169 2118 121
Size: 691 357 949 3312
Fragments arranged by size:
3312 949 691 357
PmeI GTTT'AAAC
Cuts at: 2308 2308
Size: 5309
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4448 4480 4448
Size: 32 5277
PpuMI rG'GwC_Cy
Cuts at: 1797 1797
Size: 5309
PshAI GACnn'nnGTC
Cuts at: 374 374
Size: 5309
PstI C_TGCA'G
Cuts at: 2356 3041 2356
Size: 685 4624
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5309
PvuII CAG'CTG
Cuts at: 1902 2734 3094 1902
Size: 832 360 4117
Fragments arranged by size:
4117 832 360
RsrII CG'GwC_CG
Cuts at: 3504 3504
Size: 5309
SacI G_AGCT'C
Cuts at: 1024 2323 1024
Size: 1299 4010
SalI G'TCGA_C
Cuts at: 2346 2346
Size: 5309
SanDI GG'GwC_CC
Cuts at: 1797 1797
Size: 5309
SapI GCTCTTCn'nnn_
Cuts at: 138 3332 3542 138
Size: 3194 210 1905
Fragments arranged by size:
3194 1905 210
SbfI CC_TGCA'GG
Cuts at: 2356 2356
Size: 5309
ScaI AGT'ACT
Cuts at: 4826 4826
Size: 5309
SfcI C'TryA_G
Cuts at: 33 470 2352 3037 4030 4221 33
Size: 437 1882 685 993 191 1121
Fragments arranged by size:
1882 1121 993 685 437 191
SfoI GGC'GCC
Cuts at: 2741 2989 2741
Size: 248 5061
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5309
SmaI CCC'GGG
Cuts at: 220 2331 220
Size: 2111 3198
SmlI C'TyrA_G
Cuts at: 2464 3379 3871 4133 2464
Size: 915 492 262 3640
Fragments arranged by size:
3640 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5309
SphI G_CATG'C
Cuts at: 2362 2862 2362
Size: 500 4809
StuI AGG'CCT
Cuts at: 1257 1257
Size: 5309
StyI C'CwwG_G
Cuts at: 667 1092 1205 2300 2443 2797 667
Size: 425 113 1095 143 354 3179
Fragments arranged by size:
3179 1095 425 354 143 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 171 1498 2892 3025 3484 3693 171
Size: 1327 1394 133 459 209 1787
Fragments arranged by size:
1787 1394 1327 459 209 133
TatI w'GTAC_w
Cuts at: 1889 4824 1889
Size: 2935 2374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1201 1233 2176 2208 4826 4858 1201
Size: 32 943 32 2618 32 1652
Fragments arranged by size:
2618 1652 943 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3106 3106
Size: 5309
XbaI T'CTAG_A
Cuts at: 2340 2340
Size: 5309
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 567 1155 1665 1962 2804 567
Size: 588 510 297 842 3072
Fragments arranged by size:
3072 842 588 510 297
XmaI C'CCGG_G
Cuts at: 218 2329 218
Size: 2111 3198
XmnI GAAnn'nnTTC
Cuts at: 2146 2146
Size: 5309
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PmlI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI