Restriction map for
pF1KE1125
(Circular) (Six-base) MAPSORT of: pF1KE1125.seq Check: 8266 from: 1 to: 4829
pF1KE1125 4829 bp
With 182 enzymes: SgfI *
March 23, 2015 09:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1348 1453 1348
Size: 105 4724
AccI GT'mk_AC
Cuts at: 1867 1867
Size: 4829
Acc65I G'GTAC_C
Cuts at: 1845 1845
Size: 4829
AclI AA'CG_TT
Cuts at: 4669 4669
Size: 4829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2654 3086 3833 4101 1186
Size: 1468 432 747 268 1914
Fragments arranged by size:
1914 1468 747 432 268
AflIII A'CryG_T
Cuts at: 2181 3285 4262 2181
Size: 1104 977 2748
Fragments arranged by size:
2748 1104 977
AgeI A'CCGG_T
Cuts at: 3217 3217
Size: 4829
AhdI GACnn_n'nnGTC
Cuts at: 558 558
Size: 4829
AleI CACnn'nnGTG
Cuts at: 1349 1516 1349
Size: 167 4662
AlwNI CAG_nnn'CTG
Cuts at: 732 2134 3701 732
Size: 1402 1567 1860
Fragments arranged by size:
1860 1567 1402
ApaI G_GGCC'C
Cuts at: 264 872 1149 1157 264
Size: 608 277 8 3936
Fragments arranged by size:
3936 608 277 8
ApaLI G'TGCA_C
Cuts at: 3599 3599
Size: 4829
ApoI r'AATT_y
Cuts at: 1833 4182 4438 1833
Size: 2349 256 2224
Fragments arranged by size:
2349 2224 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4829
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
AvaI C'yCGr_G
Cuts at: 229 256 605 863 1076 1283 1611 1849 229
Size: 27 349 258 213 207 328 238 3209
Fragments arranged by size:
3209 349 328 258 238 213 207 27
BamHI G'GATC_C
Cuts at: 1854 1854
Size: 4829
BanI G'GyrC_C
Cuts at: 252 379 998 1395 1543 1845 2259 2507 2542
Size: 127 619 397 148 302 414 248 35
Cuts at: 2542 252
Size: 2539
Fragments arranged by size:
2539 619 414 397 302 248 148 127
35
BanII G_rGCy'C
Cuts at: 264 872 960 1149 1157 1457 1637 1818 1843
Size: 608 88 189 8 300 180 181 25
Cuts at: 1843 2873 264
Size: 1030 2220
Fragments arranged by size:
2220 1030 608 300 189 181 180 88
25 8
BbeI G_GCGC'C
Cuts at: 1399 2263 2511 1399
Size: 864 248 3717
Fragments arranged by size:
3717 864 248
BbsI GAAGACnn'nnnn_
Cuts at: 320 320
Size: 4829
BbvCI CC'TCA_GC
Cuts at: 1474 1474
Size: 4829
BciVI GTATCCnnnnn_n'
Cuts at: 2727 3488 4829 2727
Size: 761 1341 2727
Fragments arranged by size:
2727 1341 761
BclI T'GATC_A
Cuts at: 2349 2349
Size: 4829
BglI GCCn_nnn'nGGC
Cuts at: 112 287 867 112
Size: 175 580 4074
Fragments arranged by size:
4074 580 175
BglII A'GATC_T
Cuts at: 443 2344 4381 443
Size: 1901 2037 891
Fragments arranged by size:
2037 1901 891
BlpI GC'TnA_GC
Cuts at: 1941 1941
Size: 4829
Bme1580I G_kGCm'C
Cuts at: 264 629 688 872 1001 1106 1149 1157 1546
Size: 365 59 184 129 105 43 8 389
Cuts at: 1546 2454 2547 3603 264
Size: 908 93 1056 1490
Fragments arranged by size:
1490 1056 908 389 365 184 129 105
93 59 43 8
BmrI ACTGGGnnnn_n'
Cuts at: 2041 2122 2457 4601 2041
Size: 81 335 2144 2269
Fragments arranged by size:
2269 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 570 1563 3196 570
Size: 993 1633 2203
Fragments arranged by size:
2203 1633 993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 875 893 974 1204 1348 1408 1450 1558
Size: 421 18 81 230 144 60 42 108
Cuts at: 1558 454
Size: 3725
Fragments arranged by size:
3725 421 230 144 108 81 60 42
18
Bpu10I CC'TnA_GC
Cuts at: 1474 1646 1474
Size: 172 4657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2005 2884 3376 3674 2005
Size: 879 492 298 3160
Fragments arranged by size:
3160 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 153 4533 153
Size: 4380 449
BsaAI yAC'GTr
Cuts at: 1785 2182 1785
Size: 397 4432
BsaBI GATnn'nnATC
Cuts at: 6 2343 4380 6
Size: 2337 2037 455
Fragments arranged by size:
2337 2037 455
BsaHI Gr'CG_yC
Cuts at: 1396 2260 2508 3210 4706 1396
Size: 864 248 702 1496 1519
Fragments arranged by size:
1519 1496 864 702 248
BsaWI w'CCGG_w
Cuts at: 468 2018 2539 3217 3491 3638 4049 468
Size: 1550 521 678 274 147 411 1248
Fragments arranged by size:
1550 1248 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 454 455 487 490 1192 451
Size: 3 1 32 3 702 4088
Fragments arranged by size:
4088 702 32 3 3 1
BseYI C'CCAG_C
Cuts at: 263 343 725 785 791 833 857 873 1042
Size: 80 382 60 6 42 24 16 169
Cuts at: 1042 1058 1196 2254 3012 3589 4085 263
Size: 16 138 1058 758 577 496 1007
Fragments arranged by size:
1058 1007 758 577 496 382 169 138
80 60 42 24 16 16 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1102
Size: 4829
BsiEI CG_ry'CG
Cuts at: 104 407 1263 2371 2417 3625 104
Size: 303 856 1108 46 1208 1308
Fragments arranged by size:
1308 1208 1108 856 303 46
BsiHKAI G_wGCw'C
Cuts at: 372 1621 1790 1843 2621 2811 3603 372
Size: 1249 169 53 778 190 792 1598
Fragments arranged by size:
1598 1249 792 778 190 169 53
BsmBI CGTCTCn'nnnn_
Cuts at: 131 131
Size: 4829
Bsp1286I G_dGCh'C
Cuts at: 264 372 629 688 872 960 1001 1106 1149
Size: 108 257 59 184 88 41 105 43
Cuts at: 1149 1157 1457 1546 1621 1637 1790 1818 1843
Size: 8 300 89 75 16 153 28 25
Cuts at: 1843 2454 2547 2621 2811 2873 3603 264
Size: 611 93 74 190 62 730 1490
Fragments arranged by size:
1490 730 611 300 257 190 184 153
108 105 93 89 88 75 74 62
59 43 41 28 25 16 8
BspEI T'CCGG_A
Cuts at: 468 4049 468
Size: 3581 1248
BspHI T'CATG_A
Cuts at: 4005 4005
Size: 4829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1348 1453 1879 2093 2395 2776 1348
Size: 105 426 214 302 381 3401
Fragments arranged by size:
3401 426 381 302 214 105
BsrBI CCG'CTC
Cuts at: 50 3121 3175 4659 4824 50
Size: 3071 54 1484 165 55
Fragments arranged by size:
3071 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2741 2741
Size: 4829
BsrFI r'CCGG_y
Cuts at: 1110 1571 2827 3008 3217 1110
Size: 461 1256 181 209 2722
Fragments arranged by size:
2722 1256 461 209 181
BssHII G'CGCG_C
Cuts at: 267 267
Size: 4829
BssSI C'ACGA_G
Cuts at: 3100 3458 3100
Size: 358 4471
BstAPI GCAn_nnn'nTGC
Cuts at: 367 1101 1206 2206 367
Size: 734 105 1000 2990
Fragments arranged by size:
2990 1000 734 105
BstBI TT'CG_AA
Cuts at: 3190 3190
Size: 4829
BstYI r'GATC_y
Cuts at: 1 443 967 1854 2321 2344 2679 2925 3937
Size: 442 524 887 467 23 335 246 1012
Cuts at: 3937 4023 4035 4381 1
Size: 86 12 346 449
Fragments arranged by size:
1012 887 524 467 449 442 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 488 803 488
Size: 315 4514
BtgI C'CryG_G
Cuts at: 107 1481 107
Size: 1374 3455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1311 2779 2960 3123 1311
Size: 1468 181 163 3017
Fragments arranged by size:
3017 1468 181 163
BtsI GCAGTG_nn'
Cuts at: 565 1503 1805 2072 565
Size: 938 302 267 3322
Fragments arranged by size:
3322 938 302 267
DraI TTT'AAA
Cuts at: 1828 1828
Size: 4829
DraIII CAC_nnn'GTG
Cuts at: 1101 1101
Size: 4829
DrdI GACnn_nn'nnGTC
Cuts at: 2535 3393 2535
Size: 858 3971
EaeI y'GGCC_r
Cuts at: 638 1260 1653 2414 2588 2979 3006 4351 638
Size: 622 393 761 174 391 27 1345 1116
Fragments arranged by size:
1345 1116 761 622 393 391 174 27
EagI C'GGCC_G
Cuts at: 1260 2414 1260
Size: 1154 3675
EarI CTCTTCn'nnn_
Cuts at: 2852 3062 2852
Size: 210 4619
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3037 3347 3493 4639 3037
Size: 310 146 1146 3227
Fragments arranged by size:
3227 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1841 1841
Size: 4829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 875 893 974 1186 1204 1348 1408 1450
Size: 421 18 81 212 18 144 60 42
Cuts at: 1450 1558 2654 3086 3833 4101 454
Size: 108 1096 432 747 268 1182
Fragments arranged by size:
1182 1096 747 432 421 268 212 144
108 81 60 42 18 18
EcoO109I rG'GnC_Cy
Cuts at: 260 484 492 868 869 1145 1146 1153 1154
Size: 224 8 376 1 276 1 7 1
Cuts at: 1154 1968 260
Size: 814 3121
Fragments arranged by size:
3121 814 376 276 224 8 7 1
1 1
EcoRI G'AATT_C
Cuts at: 1833 1833
Size: 4829
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2848 2848
Size: 4829
FspI TGC'GCA
Cuts at: 1355 2610 1355
Size: 1255 3574
HaeII r_GCGC'y
Cuts at: 1399 2263 2511 3533 4512 1399
Size: 864 248 1022 979 1716
Fragments arranged by size:
1716 1022 979 864 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 540 544 572 576 2671 2701 2703 2733 540
Size: 4 28 4 2095 30 2 30 2636
Fragments arranged by size:
2636 2095 30 30 28 4 4 2
HincII GTy'rAC
Cuts at: 1248 1868 1248
Size: 620 4209
KasI G'GCGC_C
Cuts at: 1395 2259 2507 1395
Size: 864 248 3717
Fragments arranged by size:
3717 864 248
KpnI G_GTAC'C
Cuts at: 1849 1849
Size: 4829
MluI A'CGCG_T
Cuts at: 4262 4262
Size: 4829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 834 1774 3500 3684 834
Size: 940 1726 184 1979
Fragments arranged by size:
1979 1726 940 184
MscI TGG'CCA
Cuts at: 640 2590 640
Size: 1950 2879
MslI CAynn'nnrTG
Cuts at: 1349 1516 2818 2945 1349
Size: 167 1302 127 3233
Fragments arranged by size:
3233 1302 167 127
MspA1I CmG'CkG
Cuts at: 1191 1317 1940 2254 2614 3627 3872 1191
Size: 126 623 314 360 1013 245 2148
Fragments arranged by size:
2148 1013 623 360 314 245 126
NaeI GCC'GGC
Cuts at: 3010 3010
Size: 4829
NarI GG'CG_CC
Cuts at: 1396 2260 2508 1396
Size: 864 248 3717
Fragments arranged by size:
3717 864 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4829
NgoMIV G'CCGG_C
Cuts at: 3008 3008
Size: 4829
NspI r_CATG'y
Cuts at: 1473 1882 2382 3289 1473
Size: 409 500 907 3013
Fragments arranged by size:
3013 907 500 409
PasI CC'CwG_GG
Cuts at: 518 1141 1150 518
Size: 623 9 4197
Fragments arranged by size:
4197 623 9
PciI A'CATG_T
Cuts at: 3285 3285
Size: 4829
PfoI T'CCnGG_A
Cuts at: 951 951
Size: 4829
PmeI GTTT'AAAC
Cuts at: 1828 1828
Size: 4829
PmlI CAC'GTG
Cuts at: 1785 1785
Size: 4829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 4797
PspOMI G'GGCC_C
Cuts at: 260 868 1145 1153 260
Size: 608 277 8 3936
Fragments arranged by size:
3936 608 277 8
PstI C_TGCA'G
Cuts at: 678 1876 2561 678
Size: 1198 685 2946
Fragments arranged by size:
2946 1198 685
PvuI CG_AT'CG
Cuts at: 104 407 104
Size: 303 4526
PvuII CAG'CTG
Cuts at: 1191 1317 2254 2614 1191
Size: 126 937 360 3406
Fragments arranged by size:
3406 937 360 126
RsrII CG'GwC_CG
Cuts at: 3024 3024
Size: 4829
SacI G_AGCT'C
Cuts at: 1843 1843
Size: 4829
SalI G'TCGA_C
Cuts at: 1866 1866
Size: 4829
SapI GCTCTTCn'nnn_
Cuts at: 2852 3062 2852
Size: 210 4619
SbfI CC_TGCA'GG
Cuts at: 1876 1876
Size: 4829
ScaI AGT'ACT
Cuts at: 4346 4346
Size: 4829
SexAI A'CCwGG_T
Cuts at: 1127 1127
Size: 4829
SfcI C'TryA_G
Cuts at: 33 674 1380 1872 2557 3550 3741 33
Size: 641 706 492 685 993 191 1121
Fragments arranged by size:
1121 993 706 685 641 492 191
SfoI GGC'GCC
Cuts at: 1397 2261 2509 1397
Size: 864 248 3717
Fragments arranged by size:
3717 864 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
SmaI CCC'GGG
Cuts at: 231 258 607 1285 1851 231
Size: 27 349 678 566 3209
Fragments arranged by size:
3209 678 566 349 27
SmlI C'TyrA_G
Cuts at: 1984 2899 3391 3653 1984
Size: 915 492 262 3160
Fragments arranged by size:
3160 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4829
SphI G_CATG'C
Cuts at: 1473 1882 2382 1473
Size: 409 500 3920
Fragments arranged by size:
3920 500 409
SrfI GCCC'GGGC
Cuts at: 1285 1285
Size: 4829
StuI AGG'CCT
Cuts at: 1492 1492
Size: 4829
StyI C'CwwG_G
Cuts at: 107 703 1963 2317 107
Size: 596 1260 354 2619
Fragments arranged by size:
2619 1260 596 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 135 871 2412 2545 3004 3213 135
Size: 736 1541 133 459 209 1751
Fragments arranged by size:
1751 1541 736 459 209 133
TatI w'GTAC_w
Cuts at: 4344 4344
Size: 4829
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4346 4378 4346
Size: 32 4797
Tth111I GACn'n_nGTC
Cuts at: 2626 2626
Size: 4829
XbaI T'CTAG_A
Cuts at: 1860 1860
Size: 4829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2324 2324
Size: 4829
XmaI C'CCGG_G
Cuts at: 229 256 605 1283 1849 229
Size: 27 349 678 566 3209
Fragments arranged by size:
3209 678 566 349 27
XmnI GAAnn'nnTTC
Cuts at: 181 220 317 416 181
Size: 39 97 99 4594
Fragments arranged by size:
4594 99 97 39
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PmlI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsrGI
BstEII BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SspI SwaI XhoI
ZraI