Restriction map for
pF1KE1099
(Circular) (Six-base) MAPSORT of: pF1KE1099.seq Check: 3654 from: 1 to: 4073
pF1KE1099 4073 bp
With 182 enzymes: SgfI *
November 19, 2009 09:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 261 261
Size: 4073
AccI GT'mk_AC
Cuts at: 1111 1111
Size: 4073
Acc65I G'GTAC_C
Cuts at: 1089 1089
Size: 4073
AclI AA'CG_TT
Cuts at: 3913 3913
Size: 4073
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 1898 2330 3077 3345 518
Size: 1380 432 747 268 1246
Fragments arranged by size:
1380 1246 747 432 268
AflIII A'CryG_T
Cuts at: 1065 1425 2529 3506 1065
Size: 360 1104 977 1632
Fragments arranged by size:
1632 1104 977 360
AgeI A'CCGG_T
Cuts at: 2461 2461
Size: 4073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 368 368
Size: 4073
AlwNI CAG_nnn'CTG
Cuts at: 838 1378 2945 838
Size: 540 1567 1966
Fragments arranged by size:
1966 1567 540
ApaLI G'TGCA_C
Cuts at: 2843 2843
Size: 4073
ApoI r'AATT_y
Cuts at: 993 1077 3426 3682 993
Size: 84 2349 256 1384
Fragments arranged by size:
2349 1384 256 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4073
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
AvaI C'yCGr_G
Cuts at: 1093 1093
Size: 4073
BamHI G'GATC_C
Cuts at: 1098 1098
Size: 4073
BanI G'GyrC_C
Cuts at: 1089 1503 1751 1786 1089
Size: 414 248 35 3376
Fragments arranged by size:
3376 414 248 35
BanII G_rGCy'C
Cuts at: 1087 2117 1087
Size: 1030 3043
BbeI G_GCGC'C
Cuts at: 1507 1755 1507
Size: 248 3825
BbsI GAAGACnn'nnnn_
Cuts at: 841 841
Size: 4073
BbvCI CC'TCA_GC
Cuts at: 113 113
Size: 4073
BciVI GTATCCnnnnn_n'
Cuts at: 1971 2732 4073 1971
Size: 761 1341 1971
Fragments arranged by size:
1971 1341 761
BclI T'GATC_A
Cuts at: 627 1593 627
Size: 966 3107
BglII A'GATC_T
Cuts at: 1588 3625 1588
Size: 2037 2036
BlpI GC'TnA_GC
Cuts at: 1185 1185
Size: 4073
Bme1580I G_kGCm'C
Cuts at: 1698 1791 2847 1698
Size: 93 1056 2924
Fragments arranged by size:
2924 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1285 1366 1701 3845 1285
Size: 81 335 2144 1513
Fragments arranged by size:
2144 1513 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 665
Size: 4073
Bpu10I CC'TnA_GC
Cuts at: 113 113
Size: 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1249 2128 2620 2918 394
Size: 855 879 492 298 1549
Fragments arranged by size:
1549 879 855 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3777 3777
Size: 4073
BsaAI yAC'GTr
Cuts at: 1426 1426
Size: 4073
BsaBI GATnn'nnATC
Cuts at: 6 1587 3624 6
Size: 1581 2037 455
Fragments arranged by size:
2037 1581 455
BsaHI Gr'CG_yC
Cuts at: 846 1504 1752 2454 3950 846
Size: 658 248 702 1496 969
Fragments arranged by size:
1496 969 702 658 248
BsaWI w'CCGG_w
Cuts at: 1262 1783 2461 2735 2882 3293 1262
Size: 521 678 274 147 411 2042
Fragments arranged by size:
2042 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 1018 196
Size: 822 3251
BseYI C'CCAG_C
Cuts at: 1498 2256 2833 3329 1498
Size: 758 577 496 2242
Fragments arranged by size:
2242 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1615 1661 2869 104
Size: 1511 46 1208 1308
Fragments arranged by size:
1511 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1087 1865 2055 2847 1087
Size: 778 190 792 2313
Fragments arranged by size:
2313 792 778 190
BsmI GAATG_Cn'
Cuts at: 330 635 330
Size: 305 3768
Bsp1286I G_dGCh'C
Cuts at: 1087 1698 1791 1865 2055 2117 2847 1087
Size: 611 93 74 190 62 730 2313
Fragments arranged by size:
2313 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3293 3293
Size: 4073
BspHI T'CATG_A
Cuts at: 3249 3249
Size: 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 261 1123 1337 1639 2020 261
Size: 862 214 302 381 2314
Fragments arranged by size:
2314 862 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2365 2419 3903 4068 50
Size: 2315 54 1484 165 55
Fragments arranged by size:
2315 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 167 1985 167
Size: 1818 2255
BsrFI r'CCGG_y
Cuts at: 2071 2252 2461 2071
Size: 181 209 3683
Fragments arranged by size:
3683 209 181
BssSI C'ACGA_G
Cuts at: 2344 2702 2344
Size: 358 3715
BstAPI GCAn_nnn'nTGC
Cuts at: 276 1450 276
Size: 1174 2899
BstBI TT'CG_AA
Cuts at: 2434 2434
Size: 4073
BstYI r'GATC_y
Cuts at: 1 1098 1565 1588 1923 2169 3181 3267 3279
Size: 1097 467 23 335 246 1012 86 12
Cuts at: 3279 3625 1
Size: 346 449
Fragments arranged by size:
1097 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1001 1001
Size: 4073
BtgI C'CryG_G
Cuts at: 107 107
Size: 4073
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2023 2204 2367 2023
Size: 181 163 3729
Fragments arranged by size:
3729 181 163
BtsI GCAGTG_nn'
Cuts at: 1316 1316
Size: 4073
DraI TTT'AAA
Cuts at: 942 1072 942
Size: 130 3943
DrdI GACnn_nn'nnGTC
Cuts at: 1779 2637 1779
Size: 858 3215
EaeI y'GGCC_r
Cuts at: 1658 1832 2223 2250 3595 1658
Size: 174 391 27 1345 2136
Fragments arranged by size:
2136 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1658 1658
Size: 4073
EarI CTCTTCn'nnn_
Cuts at: 494 1048 2096 2306 494
Size: 554 1048 210 2261
Fragments arranged by size:
2261 1048 554 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2281 2591 2737 3883 2281
Size: 310 146 1146 2471
Fragments arranged by size:
2471 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1085 1085
Size: 4073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 665 1898 2330 3077 3345 518
Size: 147 1233 432 747 268 1246
Fragments arranged by size:
1246 1233 747 432 268 147
EcoO109I rG'GnC_Cy
Cuts at: 1212 1212
Size: 4073
EcoRI G'AATT_C
Cuts at: 1077 1077
Size: 4073
EcoRV GAT'ATC
Cuts at: 579 579
Size: 4073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 677 2092 677
Size: 1415 2658
FspI TGC'GCA
Cuts at: 1854 1854
Size: 4073
HaeII r_GCGC'y
Cuts at: 1507 1755 2777 3756 1507
Size: 248 1022 979 1824
Fragments arranged by size:
1824 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 994 1026 1915 1945 1947 1977 994
Size: 32 889 30 2 30 3090
Fragments arranged by size:
3090 889 32 30 30 2
HincII GTy'rAC
Cuts at: 1112 1112
Size: 4073
KasI G'GCGC_C
Cuts at: 1503 1751 1503
Size: 248 3825
KpnI G_GTAC'C
Cuts at: 1093 1093
Size: 4073
MluI A'CGCG_T
Cuts at: 3506 3506
Size: 4073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2744 2928 2744
Size: 184 3889
MscI TGG'CCA
Cuts at: 1834 1834
Size: 4073
MslI CAynn'nnrTG
Cuts at: 973 2062 2189 973
Size: 1089 127 2857
Fragments arranged by size:
2857 1089 127
MspA1I CmG'CkG
Cuts at: 838 1184 1498 1858 2871 3116 838
Size: 346 314 360 1013 245 1795
Fragments arranged by size:
1795 1013 360 346 314 245
NaeI GCC'GGC
Cuts at: 2254 2254
Size: 4073
NarI GG'CG_CC
Cuts at: 1504 1752 1504
Size: 248 3825
NcoI C'CATG_G
Cuts at: 107 107
Size: 4073
NgoMIV G'CCGG_C
Cuts at: 2252 2252
Size: 4073
NspI r_CATG'y
Cuts at: 542 1069 1126 1626 2533 542
Size: 527 57 500 907 2082
Fragments arranged by size:
2082 907 527 500 57
PciI A'CATG_T
Cuts at: 1065 2529 1065
Size: 1464 2609
PmeI GTTT'AAAC
Cuts at: 1072 1072
Size: 4073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 4041
PstI C_TGCA'G
Cuts at: 1120 1805 1120
Size: 685 3388
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4073
PvuII CAG'CTG
Cuts at: 838 1498 1858 838
Size: 660 360 3053
Fragments arranged by size:
3053 660 360
RsrII CG'GwC_CG
Cuts at: 2268 2268
Size: 4073
SacI G_AGCT'C
Cuts at: 1087 1087
Size: 4073
SalI G'TCGA_C
Cuts at: 1110 1110
Size: 4073
SapI GCTCTTCn'nnn_
Cuts at: 2096 2306 2096
Size: 210 3863
SbfI CC_TGCA'GG
Cuts at: 1120 1120
Size: 4073
ScaI AGT'ACT
Cuts at: 660 3590 660
Size: 2930 1143
SfcI C'TryA_G
Cuts at: 33 771 1116 1801 2794 2985 33
Size: 738 345 685 993 191 1121
Fragments arranged by size:
1121 993 738 685 345 191
SfoI GGC'GCC
Cuts at: 1505 1753 1505
Size: 248 3825
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
SmaI CCC'GGG
Cuts at: 1095 1095
Size: 4073
SmlI C'TyrA_G
Cuts at: 373 1228 2143 2635 2897 373
Size: 855 915 492 262 1549
Fragments arranged by size:
1549 915 855 492 262
SpeI A'CTAG_T
Cuts at: 67 972 67
Size: 905 3168
SphI G_CATG'C
Cuts at: 1126 1626 1126
Size: 500 3573
SspI AAT'ATT
Cuts at: 215 215
Size: 4073
StyI C'CwwG_G
Cuts at: 107 331 1207 1561 107
Size: 224 876 354 2619
Fragments arranged by size:
2619 876 354 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 411 665 769 1656 1789 2248 2457 411
Size: 254 104 887 133 459 209 2027
Fragments arranged by size:
2027 887 459 254 209 133 104
TatI w'GTAC_w
Cuts at: 658 3588 658
Size: 2930 1143
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3590 3622 3590
Size: 32 4041
Tth111I GACn'n_nGTC
Cuts at: 1870 1870
Size: 4073
XbaI T'CTAG_A
Cuts at: 1104 1104
Size: 4073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1568 1568
Size: 4073
XmaI C'CCGG_G
Cuts at: 1093 1093
Size: 4073
XmnI GAAnn'nnTTC
Cuts at: 407 407
Size: 4073
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI