Restriction map for
pF1KE1095
(Circular) (Six-base) MAPSORT of: pF1KE1095.seq Check: 7172 from: 1 to: 4193
pF1KE1095 4193 bp
With 182 enzymes: SgfI *
October 26, 2009 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 237 237
Size: 4193
AccI GT'mk_AC
Cuts at: 1231 1231
Size: 4193
Acc65I G'GTAC_C
Cuts at: 748 1209 748
Size: 461 3732
AclI AA'CG_TT
Cuts at: 4033 4033
Size: 4193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 825 2018 2450 3197 3465 484
Size: 341 1193 432 747 268 1212
Fragments arranged by size:
1212 1193 747 432 341 268
AflIII A'CryG_T
Cuts at: 1154 1545 2649 3626 1154
Size: 391 1104 977 1721
Fragments arranged by size:
1721 1104 977 391
AgeI A'CCGG_T
Cuts at: 2581 2581
Size: 4193
AleI CACnn'nnGTG
Cuts at: 602 724 602
Size: 122 4071
AlwNI CAG_nnn'CTG
Cuts at: 721 1498 3065 721
Size: 777 1567 1849
Fragments arranged by size:
1849 1567 777
ApaLI G'TGCA_C
Cuts at: 2963 2963
Size: 4193
ApoI r'AATT_y
Cuts at: 1197 3546 3802 1197
Size: 2349 256 1588
Fragments arranged by size:
2349 1588 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 362 527 591 1213 362
Size: 165 64 622 3342
Fragments arranged by size:
3342 622 165 64
BamHI G'GATC_C
Cuts at: 1218 1218
Size: 4193
BanI G'GyrC_C
Cuts at: 646 748 1209 1623 1871 1906 646
Size: 102 461 414 248 35 2933
Fragments arranged by size:
2933 461 414 248 102 35
BanII G_rGCy'C
Cuts at: 215 548 1207 2237 215
Size: 333 659 1030 2171
Fragments arranged by size:
2171 1030 659 333
BbeI G_GCGC'C
Cuts at: 1627 1875 1627
Size: 248 3945
BbsI GAAGACnn'nnnn_
Cuts at: 1107 1107
Size: 4193
BbvCI CC'TCA_GC
Cuts at: 732 732
Size: 4193
BciVI GTATCCnnnnn_n'
Cuts at: 903 2091 2852 4193 903
Size: 1188 761 1341 903
Fragments arranged by size:
1341 1188 903 761
BclI T'GATC_A
Cuts at: 1713 1713
Size: 4193
BglII A'GATC_T
Cuts at: 335 1708 3745 335
Size: 1373 2037 783
Fragments arranged by size:
2037 1373 783
BlpI GC'TnA_GC
Cuts at: 1305 1305
Size: 4193
Bme1580I G_kGCm'C
Cuts at: 1818 1911 2967 1818
Size: 93 1056 3044
Fragments arranged by size:
3044 1056 93
BmgBI CAC'GTC
Cuts at: 1155 1155
Size: 4193
BmrI ACTGGGnnnn_n'
Cuts at: 1405 1486 1821 3965 1405
Size: 81 335 2144 1633
Fragments arranged by size:
2144 1633 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 589 418
Size: 171 4022
Bpu10I CC'TnA_GC
Cuts at: 732 732
Size: 4193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1369 2248 2740 3038 889
Size: 480 879 492 298 2044
Fragments arranged by size:
2044 879 492 480 298
BsaI GGTCTCn'nnnn_
Cuts at: 3897 3897
Size: 4193
BsaAI yAC'GTr
Cuts at: 978 1546 978
Size: 568 3625
BsaBI GATnn'nnATC
Cuts at: 6 454 1707 3744 6
Size: 448 1253 2037 455
Fragments arranged by size:
2037 1253 455 448
BsaHI Gr'CG_yC
Cuts at: 1624 1872 2574 4070 1624
Size: 248 702 1496 1747
Fragments arranged by size:
1747 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1382 1903 2581 2855 3002 3413 1382
Size: 521 678 274 147 411 2162
Fragments arranged by size:
2162 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 838 868 913 943 838
Size: 30 45 30 4088
Fragments arranged by size:
4088 45 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 143 843 143
Size: 700 3493
BseYI C'CCAG_C
Cuts at: 562 1618 2376 2953 3449 562
Size: 1056 758 577 496 1306
Fragments arranged by size:
1306 1056 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 262
Size: 4193
BsiEI CG_ry'CG
Cuts at: 104 1047 1735 1781 2989 104
Size: 943 688 46 1208 1308
Fragments arranged by size:
1308 1208 943 688 46
BsiHKAI G_wGCw'C
Cuts at: 637 1207 1985 2175 2967 637
Size: 570 778 190 792 1863
Fragments arranged by size:
1863 792 778 570 190
Bsp1286I G_dGCh'C
Cuts at: 215 548 637 1207 1818 1911 1985 2175 2237
Size: 333 89 570 611 93 74 190 62
Cuts at: 2237 2967 215
Size: 730 1441
Fragments arranged by size:
1441 730 611 570 333 190 93 89
74 62
BspEI T'CCGG_A
Cuts at: 3413 3413
Size: 4193
BspHI T'CATG_A
Cuts at: 3369 3369
Size: 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 237 1243 1457 1759 2140 237
Size: 1006 214 302 381 2290
Fragments arranged by size:
2290 1006 381 302 214
BsrBI CCG'CTC
Cuts at: 50 284 929 2485 2539 4023 4188 50
Size: 234 645 1556 54 1484 165 55
Fragments arranged by size:
1556 1484 645 234 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1076 2105 1076
Size: 1029 3164
BsrFI r'CCGG_y
Cuts at: 2191 2372 2581 2191
Size: 181 209 3803
Fragments arranged by size:
3803 209 181
BssSI C'ACGA_G
Cuts at: 241 2464 2822 241
Size: 2223 358 1612
Fragments arranged by size:
2223 1612 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 1570
Size: 4193
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 294 294
Size: 4193
BstYI r'GATC_y
Cuts at: 1 335 1218 1685 1708 2043 2289 3301 3387
Size: 334 883 467 23 335 246 1012 86
Cuts at: 3387 3399 3745 1
Size: 12 346 449
Fragments arranged by size:
1012 883 467 449 346 335 334 246
86 23 12
BtgI C'CryG_G
Cuts at: 179 179
Size: 4193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2143 2324 2487 2143
Size: 181 163 3849
Fragments arranged by size:
3849 181 163
BtsI GCAGTG_nn'
Cuts at: 1436 1436
Size: 4193
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DrdI GACnn_nn'nnGTC
Cuts at: 1899 2757 1899
Size: 858 3335
EaeI y'GGCC_r
Cuts at: 1778 1952 2343 2370 3715 1778
Size: 174 391 27 1345 2256
Fragments arranged by size:
2256 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2401 2711 2857 4003 2401
Size: 310 146 1146 2591
Fragments arranged by size:
2591 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 484 589 825 2018 2450 3197 3465 418
Size: 66 105 236 1193 432 747 268 1146
Fragments arranged by size:
1193 1146 747 432 268 236 105 66
EcoO109I rG'GnC_Cy
Cuts at: 958 1097 1332 958
Size: 139 235 3819
Fragments arranged by size:
3819 235 139
EcoRI G'AATT_C
Cuts at: 1197 1197
Size: 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 4193
FspI TGC'GCA
Cuts at: 196 433 1974 196
Size: 237 1541 2415
Fragments arranged by size:
2415 1541 237
FspAI rTGC'GCAy
Cuts at: 196 433 196
Size: 237 3956
HaeII r_GCGC'y
Cuts at: 1627 1875 2897 3876 1627
Size: 248 1022 979 1944
Fragments arranged by size:
1944 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 760 792 2035 2065 2067 2097 760
Size: 32 1243 30 2 30 2856
Fragments arranged by size:
2856 1243 32 30 30 2
HincII GTy'rAC
Cuts at: 1068 1232 1068
Size: 164 4029
KasI G'GCGC_C
Cuts at: 1623 1871 1623
Size: 248 3945
KpnI G_GTAC'C
Cuts at: 752 1213 752
Size: 461 3732
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 2864 3048 311
Size: 2553 184 1456
Fragments arranged by size:
2553 1456 184
MscI TGG'CCA
Cuts at: 1954 1954
Size: 4193
MslI CAynn'nnrTG
Cuts at: 478 602 683 724 1109 2182 2309 478
Size: 124 81 41 385 1073 127 2362
Fragments arranged by size:
2362 1073 385 127 124 81 41
MspA1I CmG'CkG
Cuts at: 1304 1618 1978 2991 3236 1304
Size: 314 360 1013 245 2261
Fragments arranged by size:
2261 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 1624 1872 1624
Size: 248 3945
NcoI C'CATG_G
Cuts at: 179 179
Size: 4193
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NspI r_CATG'y
Cuts at: 438 1246 1746 2653 438
Size: 808 500 907 1978
Fragments arranged by size:
1978 907 808 500
PciI A'CATG_T
Cuts at: 2649 2649
Size: 4193
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1054 1086 3332 3364 1054
Size: 32 2246 32 1883
Fragments arranged by size:
2246 1883 32 32
PpuMI rG'GwC_Cy
Cuts at: 1097 1097
Size: 4193
PstI C_TGCA'G
Cuts at: 1240 1925 1240
Size: 685 3508
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 1618 1978 1618
Size: 360 3833
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SfcI C'TryA_G
Cuts at: 33 722 1236 1921 2914 3105 33
Size: 689 514 685 993 191 1121
Fragments arranged by size:
1121 993 689 685 514 191
SfoI GGC'GCC
Cuts at: 1625 1873 1625
Size: 248 3945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 364 529 593 1215 364
Size: 165 64 622 3342
Fragments arranged by size:
3342 622 165 64
SmlI C'TyrA_G
Cuts at: 904 1348 2263 2755 3017 904
Size: 444 915 492 262 2080
Fragments arranged by size:
2080 915 492 444 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 438 1246 1746 438
Size: 808 500 2885
Fragments arranged by size:
2885 808 500
StyI C'CwwG_G
Cuts at: 179 299 380 539 779 1327 1681 179
Size: 120 81 159 240 548 354 2691
Fragments arranged by size:
2691 548 354 240 159 120 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1033 1776 1909 2368 2577 1033
Size: 743 133 459 209 2649
Fragments arranged by size:
2649 743 459 209 133
TatI w'GTAC_w
Cuts at: 482 3708 482
Size: 3226 967
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 838 870 973 1005 3710 3742 838
Size: 32 103 32 2705 32 1289
Fragments arranged by size:
2705 1289 103 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 786 1688 387
Size: 399 902 2892
Fragments arranged by size:
2892 902 399
XmaI C'CCGG_G
Cuts at: 362 527 591 1213 362
Size: 165 64 622 3342
Fragments arranged by size:
3342 622 165 64
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI