Restriction map for
pF1KE1071
(Circular) (Six-base) MAPSORT of: pF1KE1071.seq Check: 2727 from: 1 to: 5408
pF1KE1071 5408 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 2446 2446
Size: 5408
Acc65I G'GTAC_C
Cuts at: 261 2424 261
Size: 2163 3245
AclI AA'CG_TT
Cuts at: 5248 5248
Size: 5408
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 3233 3665 4412 4680 578
Size: 2655 432 747 268 1306
Fragments arranged by size:
2655 1306 747 432 268
AflIII A'CryG_T
Cuts at: 2760 3864 4841 2760
Size: 1104 977 3327
Fragments arranged by size:
3327 1104 977
AgeI A'CCGG_T
Cuts at: 3796 3796
Size: 5408
AlwNI CAG_nnn'CTG
Cuts at: 1357 1482 2713 4280 1357
Size: 125 1231 1567 2485
Fragments arranged by size:
2485 1567 1231 125
ApaI G_GGCC'C
Cuts at: 357 357
Size: 5408
ApaLI G'TGCA_C
Cuts at: 991 4178 991
Size: 3187 2221
ApoI r'AATT_y
Cuts at: 482 525 890 2247 2412 4761 5017 482
Size: 43 365 1357 165 2349 256 873
Fragments arranged by size:
2349 1357 873 365 256 165 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 5408
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5408
AvaI C'yCGr_G
Cuts at: 309 417 2428 309
Size: 108 2011 3289
Fragments arranged by size:
3289 2011 108
BamHI G'GATC_C
Cuts at: 2433 2433
Size: 5408
BanI G'GyrC_C
Cuts at: 261 2424 2838 3086 3121 261
Size: 2163 414 248 35 2548
Fragments arranged by size:
2548 2163 414 248 35
BanII G_rGCy'C
Cuts at: 357 2422 3452 357
Size: 2065 1030 2313
Fragments arranged by size:
2313 2065 1030
BbeI G_GCGC'C
Cuts at: 2842 3090 2842
Size: 248 5160
BbvCI CC'TCA_GC
Cuts at: 326 326
Size: 5408
BciVI GTATCCnnnnn_n'
Cuts at: 693 3306 4067 5408 693
Size: 2613 761 1341 693
Fragments arranged by size:
2613 1341 761 693
BclI T'GATC_A
Cuts at: 2928 2928
Size: 5408
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5408
BglII A'GATC_T
Cuts at: 456 2923 4960 456
Size: 2467 2037 904
Fragments arranged by size:
2467 2037 904
BlpI GC'TnA_GC
Cuts at: 2520 2520
Size: 5408
Bme1580I G_kGCm'C
Cuts at: 357 995 2210 3033 3126 4182 357
Size: 638 1215 823 93 1056 1583
Fragments arranged by size:
1583 1215 1056 823 638 93
BmrI ACTGGGnnnn_n'
Cuts at: 2620 2701 3036 5180 2620
Size: 81 335 2144 2848
Fragments arranged by size:
2848 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3775 3775
Size: 5408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 233 1868 212
Size: 21 1635 3752
Fragments arranged by size:
3752 1635 21
Bpu10I CC'TnA_GC
Cuts at: 326 385 326
Size: 59 5349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1211 1486 2584 3463 3955 4253 1211
Size: 275 1098 879 492 298 2366
Fragments arranged by size:
2366 1098 879 492 298 275
BsaI GGTCTCn'nnnn_
Cuts at: 836 5112 836
Size: 4276 1132
BsaAI yAC'GTr
Cuts at: 2761 2761
Size: 5408
BsaBI GATnn'nnATC
Cuts at: 6 944 1118 2922 4959 6
Size: 938 174 1804 2037 455
Fragments arranged by size:
2037 1804 938 455 174
BsaHI Gr'CG_yC
Cuts at: 2839 3087 3789 5285 2839
Size: 248 702 1496 2962
Fragments arranged by size:
2962 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2597 3118 3796 4070 4217 4628 2597
Size: 521 678 274 147 411 3377
Fragments arranged by size:
3377 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1866 1896 1866
Size: 30 5378
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 140 354 140
Size: 214 5194
BseYI C'CCAG_C
Cuts at: 2833 3591 4168 4664 2833
Size: 758 577 496 3577
Fragments arranged by size:
3577 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2102 2102
Size: 5408
BsiEI CG_ry'CG
Cuts at: 104 2950 2996 4204 104
Size: 2846 46 1208 1308
Fragments arranged by size:
2846 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 995 2422 3200 3390 4182 995
Size: 1427 778 190 792 2221
Fragments arranged by size:
2221 1427 792 778 190
BsmI GAATG_Cn'
Cuts at: 1085 1085
Size: 5408
Bsp1286I G_dGCh'C
Cuts at: 357 995 2210 2422 3033 3126 3200 3390 3452
Size: 638 1215 212 611 93 74 190 62
Cuts at: 3452 4182 357
Size: 730 1583
Fragments arranged by size:
1583 1215 730 638 611 212 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4628 4628
Size: 5408
BspHI T'CATG_A
Cuts at: 4584 4584
Size: 5408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2458 2672 2974 3355 2458
Size: 214 302 381 4511
Fragments arranged by size:
4511 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1967 3700 3754 5238 5403 50
Size: 1917 1733 54 1484 165 55
Fragments arranged by size:
1917 1733 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 619 747 949 1839 3320 619
Size: 128 202 890 1481 2707
Fragments arranged by size:
2707 1481 890 202 128
BsrFI r'CCGG_y
Cuts at: 3406 3587 3796 3406
Size: 181 209 5018
Fragments arranged by size:
5018 209 181
BssSI C'ACGA_G
Cuts at: 3679 4037 3679
Size: 358 5050
BstAPI GCAn_nnn'nTGC
Cuts at: 2785 2785
Size: 5408
BstBI TT'CG_AA
Cuts at: 3769 3769
Size: 5408
BstXI CCAn_nnnn'nTGG
Cuts at: 1984 1984
Size: 5408
BstYI r'GATC_y
Cuts at: 1 456 1056 1995 2433 2900 2923 3258 3504
Size: 455 600 939 438 467 23 335 246
Cuts at: 3504 4516 4602 4614 4960 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 939 600 467 455 449 438 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 301 1723 107
Size: 194 1422 3792
Fragments arranged by size:
3792 1422 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3358 3539 3702 3358
Size: 181 163 5064
Fragments arranged by size:
5064 181 163
BtsI GCAGTG_nn'
Cuts at: 2210 2651 2210
Size: 441 4967
DraI TTT'AAA
Cuts at: 980 2407 980
Size: 1427 3981
DraIII CAC_nnn'GTG
Cuts at: 336 336
Size: 5408
DrdI GACnn_nn'nnGTC
Cuts at: 3114 3972 3114
Size: 858 4550
EaeI y'GGCC_r
Cuts at: 1726 2993 3167 3558 3585 4930 1726
Size: 1267 174 391 27 1345 2204
Fragments arranged by size:
2204 1345 1267 391 174 27
EagI C'GGCC_G
Cuts at: 2993 2993
Size: 5408
EarI CTCTTCn'nnn_
Cuts at: 356 1487 1813 2033 3431 3641 356
Size: 1131 326 220 1398 210 2123
Fragments arranged by size:
2123 1398 1131 326 220 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1785 3616 3926 4072 5218 1785
Size: 1831 310 146 1146 1975
Fragments arranged by size:
1975 1831 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2420 2420
Size: 5408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 233 578 1868 3233 3665 4412 4680 212
Size: 21 345 1290 1365 432 747 268 940
Fragments arranged by size:
1365 1290 940 747 432 345 268 21
EcoNI CCTnn'n_nnAGG
Cuts at: 361 361
Size: 5408
EcoO109I rG'GnC_Cy
Cuts at: 239 354 2547 239
Size: 115 2193 3100
Fragments arranged by size:
3100 2193 115
EcoRI G'AATT_C
Cuts at: 2412 2412
Size: 5408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 475 3427 475
Size: 2952 2456
FspI TGC'GCA
Cuts at: 3189 3189
Size: 5408
HaeII r_GCGC'y
Cuts at: 2359 2842 3090 4112 5091 2359
Size: 483 248 1022 979 2676
Fragments arranged by size:
2676 1022 979 483 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1048 1080 1240 1272 1281 1313 1563 1595 3250
Size: 32 160 32 9 32 250 32 1655
Cuts at: 3250 3280 3282 3312 1048
Size: 30 2 30 3144
Fragments arranged by size:
3144 1655 250 160 32 32 32 32
30 30 9 2
HincII GTy'rAC
Cuts at: 584 1329 1874 2447 584
Size: 745 545 573 3545
Fragments arranged by size:
3545 745 573 545
HpaI GTT'AAC
Cuts at: 584 584
Size: 5408
KasI G'GCGC_C
Cuts at: 2838 3086 2838
Size: 248 5160
KpnI G_GTAC'C
Cuts at: 265 2428 265
Size: 2163 3245
MluI A'CGCG_T
Cuts at: 4841 4841
Size: 5408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 379 4079 4263 379
Size: 3700 184 1524
Fragments arranged by size:
3700 1524 184
MscI TGG'CCA
Cuts at: 1728 3169 1728
Size: 1441 3967
MslI CAynn'nnrTG
Cuts at: 972 3397 3524 972
Size: 2425 127 2856
Fragments arranged by size:
2856 2425 127
MspA1I CmG'CkG
Cuts at: 425 2519 2833 3193 4206 4451 425
Size: 2094 314 360 1013 245 1382
Fragments arranged by size:
2094 1382 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3589 3589
Size: 5408
NarI GG'CG_CC
Cuts at: 2839 3087 2839
Size: 248 5160
NcoI C'CATG_G
Cuts at: 107 107
Size: 5408
NgoMIV G'CCGG_C
Cuts at: 3587 3587
Size: 5408
NspI r_CATG'y
Cuts at: 1025 2461 2961 3868 1025
Size: 1436 500 907 2565
Fragments arranged by size:
2565 1436 907 500
PciI A'CATG_T
Cuts at: 3864 3864
Size: 5408
PflMI CCAn_nnn'nTGG
Cuts at: 768 768
Size: 5408
PfoI T'CCnGG_A
Cuts at: 1998 1998
Size: 5408
PmeI GTTT'AAAC
Cuts at: 2407 2407
Size: 5408
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1806 1838 4547 4579 1806
Size: 32 2709 32 2635
Fragments arranged by size:
2709 2635 32 32
PpuMI rG'GwC_Cy
Cuts at: 239 239
Size: 5408
PspOMI G'GGCC_C
Cuts at: 353 353
Size: 5408
PstI C_TGCA'G
Cuts at: 323 578 2455 3140 323
Size: 255 1877 685 2591
Fragments arranged by size:
2591 1877 685 255
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5408
PvuII CAG'CTG
Cuts at: 2833 3193 2833
Size: 360 5048
RsrII CG'GwC_CG
Cuts at: 3603 3603
Size: 5408
SacI G_AGCT'C
Cuts at: 2422 2422
Size: 5408
SalI G'TCGA_C
Cuts at: 2445 2445
Size: 5408
SanDI GG'GwC_CC
Cuts at: 239 239
Size: 5408
SapI GCTCTTCn'nnn_
Cuts at: 2033 3431 3641 2033
Size: 1398 210 3800
Fragments arranged by size:
3800 1398 210
SbfI CC_TGCA'GG
Cuts at: 2455 2455
Size: 5408
ScaI AGT'ACT
Cuts at: 1006 4925 1006
Size: 3919 1489
SfcI C'TryA_G
Cuts at: 33 319 574 2451 3136 4129 4320 33
Size: 286 255 1877 685 993 191 1121
Fragments arranged by size:
1877 1121 993 685 286 255 191
SfoI GGC'GCC
Cuts at: 2840 3088 2840
Size: 248 5160
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5408
SmaI CCC'GGG
Cuts at: 311 419 2430 311
Size: 108 2011 3289
Fragments arranged by size:
3289 2011 108
SmlI C'TyrA_G
Cuts at: 1226 1465 2563 3478 3970 4232 1226
Size: 239 1098 915 492 262 2402
Fragments arranged by size:
2402 1098 915 492 262 239
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5408
SphI G_CATG'C
Cuts at: 2461 2961 2461
Size: 500 4908
SspI AAT'ATT
Cuts at: 1014 1795 1014
Size: 781 4627
StyI C'CwwG_G
Cuts at: 107 2378 2542 2896 107
Size: 2271 164 354 2619
Fragments arranged by size:
2619 2271 354 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 265 463 901 2263 2991 3124 3583 3792 265
Size: 198 438 1362 728 133 459 209 1881
Fragments arranged by size:
1881 1362 728 459 438 209 198 133
TatI w'GTAC_w
Cuts at: 1004 4923 1004
Size: 3919 1489
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4925 4957 4925
Size: 32 5376
Tth111I GACn'n_nGTC
Cuts at: 1210 3205 1210
Size: 1995 3413
XbaI T'CTAG_A
Cuts at: 2439 2439
Size: 5408
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2217 2903 2217
Size: 686 4722
XmaI C'CCGG_G
Cuts at: 309 417 2428 309
Size: 108 2011 3289
Fragments arranged by size:
3289 2011 108
XmnI GAAnn'nnTTC
Cuts at: 2249 2249
Size: 5408
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI