Restriction map for
pF1KE1067
(Circular) (Six-base) MAPSORT of: pF1KE1067.seq Check: 1184 from: 1 to: 4631
pF1KE1067 4631 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1161 1161
Size: 4631
AccI GT'mk_AC
Cuts at: 1669 1669
Size: 4631
Acc65I G'GTAC_C
Cuts at: 1647 1647
Size: 4631
AclI AA'CG_TT
Cuts at: 4471 4471
Size: 4631
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 477 513 1078 1210 2456 2888 3635 3903
Size: 95 36 565 132 1246 432 747 268
Cuts at: 3903 382
Size: 1110
Fragments arranged by size:
1246 1110 747 565 432 268 132 95
36
AflIII A'CryG_T
Cuts at: 1983 3087 4064 1983
Size: 1104 977 2550
Fragments arranged by size:
2550 1104 977
AgeI A'CCGG_T
Cuts at: 3019 3019
Size: 4631
AhdI GACnn_n'nnGTC
Cuts at: 1542 1542
Size: 4631
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 659 659
Size: 4631
AlwNI CAG_nnn'CTG
Cuts at: 1593 1936 3503 1593
Size: 343 1567 2721
Fragments arranged by size:
2721 1567 343
ApaLI G'TGCA_C
Cuts at: 3401 3401
Size: 4631
ApoI r'AATT_y
Cuts at: 802 1635 3984 4240 802
Size: 833 2349 256 1193
Fragments arranged by size:
2349 1193 833 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4631
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4631
AvaI C'yCGr_G
Cuts at: 506 1414 1651 506
Size: 908 237 3486
Fragments arranged by size:
3486 908 237
BamHI G'GATC_C
Cuts at: 158 1656 158
Size: 1498 3133
BanI G'GyrC_C
Cuts at: 514 1647 2061 2309 2344 514
Size: 1133 414 248 35 2801
Fragments arranged by size:
2801 1133 414 248 35
BanII G_rGCy'C
Cuts at: 890 1219 1260 1469 1645 2675 890
Size: 329 41 209 176 1030 2846
Fragments arranged by size:
2846 1030 329 209 176 41
BbeI G_GCGC'C
Cuts at: 2065 2313 2065
Size: 248 4383
BbvCI CC'TCA_GC
Cuts at: 1076 1076
Size: 4631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4597
BciVI GTATCCnnnnn_n'
Cuts at: 272 2529 3290 4631 272
Size: 2257 761 1341 272
Fragments arranged by size:
2257 1341 761 272
BclI T'GATC_A
Cuts at: 2151 2151
Size: 4631
BglI GCCn_nnn'nGGC
Cuts at: 727 736 727
Size: 9 4622
BglII A'GATC_T
Cuts at: 488 2146 4183 488
Size: 1658 2037 936
Fragments arranged by size:
2037 1658 936
BlpI GC'TnA_GC
Cuts at: 619 1743 619
Size: 1124 3507
Bme1580I G_kGCm'C
Cuts at: 149 955 2256 2349 3405 149
Size: 806 1301 93 1056 1375
Fragments arranged by size:
1375 1301 1056 806 93
BmgBI CAC'GTC
Cuts at: 231 407 231
Size: 176 4455
BmrI ACTGGGnnnn_n'
Cuts at: 1603 1843 1924 2259 4403 1603
Size: 240 81 335 2144 1831
Fragments arranged by size:
2144 1831 335 240 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 747 1554 2998 747
Size: 807 1444 2380
Fragments arranged by size:
2380 1444 807
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 926 979 991 1114 1129 1255 1411 1483
Size: 189 53 12 123 15 126 156 72
Cuts at: 1483 737
Size: 3885
Fragments arranged by size:
3885 189 156 126 123 72 53 15
12
Bpu10I CC'TnA_GC
Cuts at: 690 1076 1289 690
Size: 386 213 4032
Fragments arranged by size:
4032 386 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1807 2686 3178 3476 1807
Size: 879 492 298 2962
Fragments arranged by size:
2962 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 689 4335 689
Size: 3646 985
BsaAI yAC'GTr
Cuts at: 216 1984 216
Size: 1768 2863
BsaBI GATnn'nnATC
Cuts at: 6 2145 4182 6
Size: 2139 2037 455
Fragments arranged by size:
2139 2037 455
BsaHI Gr'CG_yC
Cuts at: 179 2062 2310 3012 4508 179
Size: 1883 248 702 1496 302
Fragments arranged by size:
1883 1496 702 302 248
BsaWI w'CCGG_w
Cuts at: 1252 1820 2341 3019 3293 3440 3851 1252
Size: 568 521 678 274 147 411 2032
Fragments arranged by size:
2032 678 568 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 338 368 847 877 1492 1522 338
Size: 30 479 30 615 30 3447
Fragments arranged by size:
3447 615 479 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 763 982 994 1543 1567 763
Size: 219 12 549 24 3827
Fragments arranged by size:
3827 549 219 24 12
BseYI C'CCAG_C
Cuts at: 1317 1431 2056 2814 3391 3887 1317
Size: 114 625 758 577 496 2061
Fragments arranged by size:
2061 758 625 577 496 114
BsiEI CG_ry'CG
Cuts at: 104 2173 2219 3427 104
Size: 2069 46 1208 1308
Fragments arranged by size:
2069 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1271 1469 1645 2423 2613 3405 1271
Size: 198 176 778 190 792 2497
Fragments arranged by size:
2497 792 778 198 190 176
BsmI GAATG_Cn'
Cuts at: 648 1305 648
Size: 657 3974
Bsp1286I G_dGCh'C
Cuts at: 149 890 955 1219 1260 1271 1469 1645 2256
Size: 741 65 264 41 11 198 176 611
Cuts at: 2256 2349 2423 2613 2675 3405 149
Size: 93 74 190 62 730 1375
Fragments arranged by size:
1375 741 730 611 264 198 190 176
93 74 65 62 41 11
BspEI T'CCGG_A
Cuts at: 1252 3851 1252
Size: 2599 2032
BspHI T'CATG_A
Cuts at: 468 3807 468
Size: 3339 1292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 161 1161 1310 1681 1895 2197 2578 161
Size: 1000 149 371 214 302 381 2214
Fragments arranged by size:
2214 1000 381 371 302 214 149
BsrBI CCG'CTC
Cuts at: 50 1250 2923 2977 4461 4626 50
Size: 1200 1673 54 1484 165 55
Fragments arranged by size:
1673 1484 1200 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2543 2543
Size: 4631
BsrFI r'CCGG_y
Cuts at: 2629 2810 3019 2629
Size: 181 209 4241
Fragments arranged by size:
4241 209 181
BssSI C'ACGA_G
Cuts at: 1539 2902 3260 1539
Size: 1363 358 2910
Fragments arranged by size:
2910 1363 358
BstAPI GCAn_nnn'nTGC
Cuts at: 153 2008 153
Size: 1855 2776
BstBI TT'CG_AA
Cuts at: 2992 2992
Size: 4631
BstXI CCAn_nnnn'nTGG
Cuts at: 661 715 661
Size: 54 4577
BstYI r'GATC_y
Cuts at: 1 158 452 488 1656 2123 2146 2481 2727
Size: 157 294 36 1168 467 23 335 246
Cuts at: 2727 3739 3825 3837 4183 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1168 1012 467 449 346 335 294 246
157 86 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 1362 1362
Size: 4631
BtgI C'CryG_G
Cuts at: 734 1211 1622 734
Size: 477 411 3743
Fragments arranged by size:
3743 477 411
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1476 2581 2762 2925 1476
Size: 1105 181 163 3182
Fragments arranged by size:
3182 1105 181 163
BtsI GCAGTG_nn'
Cuts at: 740 1874 740
Size: 1134 3497
ClaI AT'CG_AT
Cuts at: 761 761
Size: 4631
DraI TTT'AAA
Cuts at: 294 546 1630 294
Size: 252 1084 3295
Fragments arranged by size:
3295 1084 252
DrdI GACnn_nn'nnGTC
Cuts at: 966 2337 3195 966
Size: 1371 858 2402
Fragments arranged by size:
2402 1371 858
EaeI y'GGCC_r
Cuts at: 651 737 2216 2390 2781 2808 4153 651
Size: 86 1479 174 391 27 1345 1129
Fragments arranged by size:
1479 1345 1129 391 174 86 27
EagI C'GGCC_G
Cuts at: 2216 2216
Size: 4631
EarI CTCTTCn'nnn_
Cuts at: 553 2654 2864 553
Size: 2101 210 2320
Fragments arranged by size:
2320 2101 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1400 2839 3149 3295 4441 1400
Size: 1439 310 146 1146 1590
Fragments arranged by size:
1590 1439 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1467 1643 1467
Size: 176 4455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 477 513 737 926 979 991 1078 1114
Size: 95 36 224 189 53 12 87 36
Cuts at: 1114 1129 1210 1255 1411 1483 2456 2888 3635
Size: 15 81 45 156 72 973 432 747
Cuts at: 3635 3903 382
Size: 268 1110
Fragments arranged by size:
1110 973 747 432 268 224 189 156
95 87 81 72 53 45 36 36
15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 906 1365 906
Size: 459 4172
EcoO109I rG'GnC_Cy
Cuts at: 438 728 774 1299 1770 438
Size: 290 46 525 471 3299
Fragments arranged by size:
3299 525 471 290 46
EcoRI G'AATT_C
Cuts at: 802 1635 802
Size: 833 3798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4631
FspI TGC'GCA
Cuts at: 2412 2412
Size: 4631
HaeII r_GCGC'y
Cuts at: 1450 2065 2313 3335 4314 1450
Size: 615 248 1022 979 1767
Fragments arranged by size:
1767 1022 979 615 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1492 1524 1528 1560 2473 2503 2505 2535 1492
Size: 32 4 32 913 30 2 30 3588
Fragments arranged by size:
3588 913 32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 1670 1670
Size: 4631
HindIII A'AGCT_T
Cuts at: 376 376
Size: 4631
KasI G'GCGC_C
Cuts at: 2061 2309 2061
Size: 248 4383
KpnI G_GTAC'C
Cuts at: 1651 1651
Size: 4631
MluI A'CGCG_T
Cuts at: 4064 4064
Size: 4631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1535 3302 3486 1535
Size: 1767 184 2680
Fragments arranged by size:
2680 1767 184
MscI TGG'CCA
Cuts at: 653 2392 653
Size: 1739 2892
MslI CAynn'nnrTG
Cuts at: 2620 2747 2620
Size: 127 4504
MspA1I CmG'CkG
Cuts at: 966 1317 1742 2056 2416 3429 3674 966
Size: 351 425 314 360 1013 245 1923
Fragments arranged by size:
1923 1013 425 360 351 314 245
NaeI GCC'GGC
Cuts at: 2812 2812
Size: 4631
NarI GG'CG_CC
Cuts at: 2062 2310 2062
Size: 248 4383
NcoI C'CATG_G
Cuts at: 1211 1211
Size: 4631
NgoMIV G'CCGG_C
Cuts at: 2810 2810
Size: 4631
NspI r_CATG'y
Cuts at: 1143 1684 2184 3091 1143
Size: 541 500 907 2683
Fragments arranged by size:
2683 907 541 500
PciI A'CATG_T
Cuts at: 3087 3087
Size: 4631
PfoI T'CCnGG_A
Cuts at: 797 797
Size: 4631
PmeI GTTT'AAAC
Cuts at: 1630 1630
Size: 4631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 3770
Size: 32 4599
PpuMI rG'GwC_Cy
Cuts at: 438 774 1299 438
Size: 336 525 3770
Fragments arranged by size:
3770 525 336
PstI C_TGCA'G
Cuts at: 629 747 1130 1583 1678 2363 629
Size: 118 383 453 95 685 2897
Fragments arranged by size:
2897 685 453 383 118 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4631
PvuII CAG'CTG
Cuts at: 2056 2416 2056
Size: 360 4271
RsrII CG'GwC_CG
Cuts at: 2826 2826
Size: 4631
SacI G_AGCT'C
Cuts at: 1469 1645 1469
Size: 176 4455
SalI G'TCGA_C
Cuts at: 1668 1668
Size: 4631
SapI GCTCTTCn'nnn_
Cuts at: 553 2654 2864 553
Size: 2101 210 2320
Fragments arranged by size:
2320 2101 210
SbfI CC_TGCA'GG
Cuts at: 1678 1678
Size: 4631
ScaI AGT'ACT
Cuts at: 4148 4148
Size: 4631
SfcI C'TryA_G
Cuts at: 33 447 625 743 833 1126 1579 1674 2359
Size: 414 178 118 90 293 453 95 685
Cuts at: 2359 3352 3543 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 453 414 293 191 178
118 95 90
SfiI GGCCn_nnn'nGGCC
Cuts at: 736 736
Size: 4631
SfoI GGC'GCC
Cuts at: 2063 2311 2063
Size: 248 4383
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4631
SmaI CCC'GGG
Cuts at: 1653 1653
Size: 4631
SmlI C'TyrA_G
Cuts at: 506 1786 2701 3193 3455 506
Size: 1280 915 492 262 1682
Fragments arranged by size:
1682 1280 915 492 262
SnaBI TAC'GTA
Cuts at: 216 216
Size: 4631
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4631
SphI G_CATG'C
Cuts at: 1684 2184 1684
Size: 500 4131
StuI AGG'CCT
Cuts at: 598 947 598
Size: 349 4282
StyI C'CwwG_G
Cuts at: 235 866 1211 1765 2119 235
Size: 631 345 554 354 2747
Fragments arranged by size:
2747 631 554 354 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 921 2214 2347 2806 3015 921
Size: 1293 133 459 209 2537
Fragments arranged by size:
2537 1293 459 209 133
TatI w'GTAC_w
Cuts at: 125 4146 125
Size: 4021 610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 338 370 391 423 847 879 4148 4180 338
Size: 32 21 32 424 32 3269 32 789
Fragments arranged by size:
3269 789 424 32 32 32 32 21
Tth111I GACn'n_nGTC
Cuts at: 2428 2428
Size: 4631
XbaI T'CTAG_A
Cuts at: 825 1662 825
Size: 837 3794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 249 2126 249
Size: 1877 2754
XhoI C'TCGA_G
Cuts at: 506 506
Size: 4631
XmaI C'CCGG_G
Cuts at: 1651 1651
Size: 4631
XmnI GAAnn'nnTTC
Cuts at: 490 490
Size: 4631
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BaeI BbsI BfrBI BmtI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SrfI SspI SwaI ZraI