Restriction map for
pF1KE1046
(Circular) (Six-base) MAPSORT of: pF1KE1046.seq Check: 7347 from: 1 to: 3548
pF1KE1046 3548 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 586 586
Size: 3548
Acc65I G'GTAC_C
Cuts at: 564 564
Size: 3548
AclI AA'CG_TT
Cuts at: 3388 3388
Size: 3548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1373 1805 2552 2820 1373
Size: 432 747 268 2101
Fragments arranged by size:
2101 747 432 268
AflIII A'CryG_T
Cuts at: 900 2004 2981 900
Size: 1104 977 1467
Fragments arranged by size:
1467 1104 977
AgeI A'CCGG_T
Cuts at: 1936 1936
Size: 3548
AlwNI CAG_nnn'CTG
Cuts at: 853 2420 853
Size: 1567 1981
ApaLI G'TGCA_C
Cuts at: 2318 2318
Size: 3548
ApoI r'AATT_y
Cuts at: 552 2901 3157 552
Size: 2349 256 943
Fragments arranged by size:
2349 943 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3548
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3548
AvaI C'yCGr_G
Cuts at: 533 568 533
Size: 35 3513
BamHI G'GATC_C
Cuts at: 573 573
Size: 3548
BanI G'GyrC_C
Cuts at: 170 564 978 1226 1261 170
Size: 394 414 248 35 2457
Fragments arranged by size:
2457 414 394 248 35
BanII G_rGCy'C
Cuts at: 482 562 1592 482
Size: 80 1030 2438
Fragments arranged by size:
2438 1030 80
BbeI G_GCGC'C
Cuts at: 982 1230 982
Size: 248 3300
BciVI GTATCCnnnnn_n'
Cuts at: 1446 2207 3548 1446
Size: 761 1341 1446
Fragments arranged by size:
1446 1341 761
BclI T'GATC_A
Cuts at: 1068 1068
Size: 3548
BglI GCCn_nnn'nGGC
Cuts at: 458 458
Size: 3548
BglII A'GATC_T
Cuts at: 1063 3100 1063
Size: 2037 1511
BlpI GC'TnA_GC
Cuts at: 660 660
Size: 3548
Bme1580I G_kGCm'C
Cuts at: 454 1173 1266 2322 454
Size: 719 93 1056 1680
Fragments arranged by size:
1680 1056 719 93
BmrI ACTGGGnnnn_n'
Cuts at: 350 760 841 1176 3320 350
Size: 410 81 335 2144 578
Fragments arranged by size:
2144 578 410 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1603 2095 2393 724
Size: 879 492 298 1879
Fragments arranged by size:
1879 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3252 3252
Size: 3548
BsaAI yAC'GTr
Cuts at: 901 901
Size: 3548
BsaBI GATnn'nnATC
Cuts at: 6 1062 3099 6
Size: 1056 2037 455
Fragments arranged by size:
2037 1056 455
BsaHI Gr'CG_yC
Cuts at: 334 979 1227 1929 3425 334
Size: 645 248 702 1496 457
Fragments arranged by size:
1496 702 645 457 248
BsaWI w'CCGG_w
Cuts at: 137 737 1258 1936 2210 2357 2768 137
Size: 600 521 678 274 147 411 917
Fragments arranged by size:
917 678 600 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 193 223 193
Size: 30 3518
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 220 217
Size: 3 3545
BseYI C'CCAG_C
Cuts at: 456 973 1731 2308 2804 456
Size: 517 758 577 496 1200
Fragments arranged by size:
1200 758 577 517 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 382 139
Size: 243 3305
BsiEI CG_ry'CG
Cuts at: 104 1090 1136 2344 104
Size: 986 46 1208 1308
Fragments arranged by size:
1308 1208 986 46
BsiHKAI G_wGCw'C
Cuts at: 482 562 1340 1530 2322 482
Size: 80 778 190 792 1708
Fragments arranged by size:
1708 792 778 190 80
BsmBI CGTCTCn'nnnn_
Cuts at: 325 325
Size: 3548
Bsp1286I G_dGCh'C
Cuts at: 454 482 562 1173 1266 1340 1530 1592 2322
Size: 28 80 611 93 74 190 62 730
Cuts at: 2322 454
Size: 1680
Fragments arranged by size:
1680 730 611 190 93 80 74 62
28
BspEI T'CCGG_A
Cuts at: 2768 2768
Size: 3548
BspHI T'CATG_A
Cuts at: 356 2724 356
Size: 2368 1180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 598 812 1114 1495 598
Size: 214 302 381 2651
Fragments arranged by size:
2651 381 302 214
BsrBI CCG'CTC
Cuts at: 50 245 1840 1894 3378 3543 50
Size: 195 1595 54 1484 165 55
Fragments arranged by size:
1595 1484 195 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1460 1460
Size: 3548
BsrFI r'CCGG_y
Cuts at: 1546 1727 1936 1546
Size: 181 209 3158
Fragments arranged by size:
3158 209 181
BssSI C'ACGA_G
Cuts at: 1819 2177 1819
Size: 358 3190
BstAPI GCAn_nnn'nTGC
Cuts at: 925 925
Size: 3548
BstBI TT'CG_AA
Cuts at: 1909 1909
Size: 3548
BstYI r'GATC_y
Cuts at: 1 573 1040 1063 1398 1644 2656 2742 2754
Size: 572 467 23 335 246 1012 86 12
Cuts at: 2754 3100 1
Size: 346 449
Fragments arranged by size:
1012 572 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1498 1679 1842 1498
Size: 181 163 3204
Fragments arranged by size:
3204 181 163
BtsI GCAGTG_nn'
Cuts at: 791 791
Size: 3548
DraI TTT'AAA
Cuts at: 547 547
Size: 3548
DrdI GACnn_nn'nnGTC
Cuts at: 1254 2112 1254
Size: 858 2690
EaeI y'GGCC_r
Cuts at: 1133 1307 1698 1725 3070 1133
Size: 174 391 27 1345 1611
Fragments arranged by size:
1611 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1133 1133
Size: 3548
EarI CTCTTCn'nnn_
Cuts at: 238 1571 1781 238
Size: 1333 210 2005
Fragments arranged by size:
2005 1333 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 519 1756 2066 2212 3358 519
Size: 1237 310 146 1146 709
Fragments arranged by size:
1237 1146 709 310 146
EcoICRI GAG'CTC
Cuts at: 480 560 480
Size: 80 3468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1373 1805 2552 2820 1373
Size: 432 747 268 2101
Fragments arranged by size:
2101 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 379 687 379
Size: 308 3240
EcoRI G'AATT_C
Cuts at: 552 552
Size: 3548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 303 1567 303
Size: 1264 2284
FspI TGC'GCA
Cuts at: 1329 1329
Size: 3548
HaeII r_GCGC'y
Cuts at: 982 1230 2252 3231 982
Size: 248 1022 979 1299
Fragments arranged by size:
1299 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1390 1420 1422 1452 1390
Size: 30 2 30 3486
Fragments arranged by size:
3486 30 30 2
HincII GTy'rAC
Cuts at: 587 587
Size: 3548
KasI G'GCGC_C
Cuts at: 978 1226 978
Size: 248 3300
KpnI G_GTAC'C
Cuts at: 568 568
Size: 3548
MluI A'CGCG_T
Cuts at: 2981 2981
Size: 3548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2219 2403 2219
Size: 184 3364
MscI TGG'CCA
Cuts at: 1309 1309
Size: 3548
MslI CAynn'nnrTG
Cuts at: 1537 1664 1537
Size: 127 3421
MspA1I CmG'CkG
Cuts at: 456 659 973 1333 2346 2591 456
Size: 203 314 360 1013 245 1413
Fragments arranged by size:
1413 1013 360 314 245 203
NaeI GCC'GGC
Cuts at: 1729 1729
Size: 3548
NarI GG'CG_CC
Cuts at: 979 1227 979
Size: 248 3300
NgoMIV G'CCGG_C
Cuts at: 1727 1727
Size: 3548
NspI r_CATG'y
Cuts at: 601 1101 2008 601
Size: 500 907 2141
Fragments arranged by size:
2141 907 500
PciI A'CATG_T
Cuts at: 2004 2004
Size: 3548
PmeI GTTT'AAAC
Cuts at: 547 547
Size: 3548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2687 2719 2687
Size: 32 3516
PpuMI rG'GwC_Cy
Cuts at: 379 379
Size: 3548
PstI C_TGCA'G
Cuts at: 595 1280 595
Size: 685 2863
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3548
PvuII CAG'CTG
Cuts at: 973 1333 973
Size: 360 3188
RsrII CG'GwC_CG
Cuts at: 1743 1743
Size: 3548
SacI G_AGCT'C
Cuts at: 482 562 482
Size: 80 3468
SalI G'TCGA_C
Cuts at: 585 585
Size: 3548
SanDI GG'GwC_CC
Cuts at: 379 379
Size: 3548
SapI GCTCTTCn'nnn_
Cuts at: 238 1571 1781 238
Size: 1333 210 2005
Fragments arranged by size:
2005 1333 210
SbfI CC_TGCA'GG
Cuts at: 595 595
Size: 3548
ScaI AGT'ACT
Cuts at: 3065 3065
Size: 3548
SfcI C'TryA_G
Cuts at: 33 159 408 591 1276 2269 2460 33
Size: 126 249 183 685 993 191 1121
Fragments arranged by size:
1121 993 685 249 191 183 126
SfoI GGC'GCC
Cuts at: 980 1228 980
Size: 248 3300
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3548
SmaI CCC'GGG
Cuts at: 535 570 535
Size: 35 3513
SmlI C'TyrA_G
Cuts at: 703 1618 2110 2372 703
Size: 915 492 262 1879
Fragments arranged by size:
1879 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3548
SphI G_CATG'C
Cuts at: 601 1101 601
Size: 500 3048
SrfI GCCC'GGGC
Cuts at: 535 535
Size: 3548
StyI C'CwwG_G
Cuts at: 197 682 1036 197
Size: 485 354 2709
Fragments arranged by size:
2709 485 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1131 1264 1723 1932 1131
Size: 133 459 209 2747
Fragments arranged by size:
2747 459 209 133
TatI w'GTAC_w
Cuts at: 3063 3063
Size: 3548
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3516
Tth111I GACn'n_nGTC
Cuts at: 1345 1345
Size: 3548
XbaI T'CTAG_A
Cuts at: 579 579
Size: 3548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1043 1043
Size: 3548
XmaI C'CCGG_G
Cuts at: 533 568 533
Size: 35 3513
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BpmI Bpu10I BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SspI StuI SwaI XhoI
XmnI ZraI