Restriction map for
pF1KE1004
(Circular) (Six-base) MAPSORT of: pF1KE1004.seq Check: 8172 from: 1 to: 3599
pF1KE1004 3599 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AccI GT'mk_AC
Cuts at: 637 637
Size: 3599
Acc65I G'GTAC_C
Cuts at: 615 615
Size: 3599
AclI AA'CG_TT
Cuts at: 3439 3439
Size: 3599
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1424 1856 2603 2871 1424
Size: 432 747 268 2152
Fragments arranged by size:
2152 747 432 268
AflIII A'CryG_T
Cuts at: 951 2055 3032 951
Size: 1104 977 1518
Fragments arranged by size:
1518 1104 977
AgeI A'CCGG_T
Cuts at: 1987 1987
Size: 3599
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 191 223 191
Size: 32 3567
AlwNI CAG_nnn'CTG
Cuts at: 176 241 904 2471 176
Size: 65 663 1567 1304
Fragments arranged by size:
1567 1304 663 65
ApaLI G'TGCA_C
Cuts at: 2369 2369
Size: 3599
ApoI r'AATT_y
Cuts at: 603 2952 3208 603
Size: 2349 256 994
Fragments arranged by size:
2349 994 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3599
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3599
AvaI C'yCGr_G
Cuts at: 619 619
Size: 3599
BamHI G'GATC_C
Cuts at: 624 624
Size: 3599
BanI G'GyrC_C
Cuts at: 615 1029 1277 1312 615
Size: 414 248 35 2902
Fragments arranged by size:
2902 414 248 35
BanII G_rGCy'C
Cuts at: 613 1643 613
Size: 1030 2569
BbeI G_GCGC'C
Cuts at: 1033 1281 1033
Size: 248 3351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3565
BciVI GTATCCnnnnn_n'
Cuts at: 1497 2258 3599 1497
Size: 761 1341 1497
Fragments arranged by size:
1497 1341 761
BclI T'GATC_A
Cuts at: 1119 1119
Size: 3599
BglII A'GATC_T
Cuts at: 1114 3151 1114
Size: 2037 1562
BlpI GC'TnA_GC
Cuts at: 711 711
Size: 3599
Bme1580I G_kGCm'C
Cuts at: 1224 1317 2373 1224
Size: 93 1056 2450
Fragments arranged by size:
2450 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 186 811 892 1227 3371 186
Size: 625 81 335 2144 414
Fragments arranged by size:
2144 625 414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1966 1966
Size: 3599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 158
Size: 3599
Bpu10I CC'TnA_GC
Cuts at: 131 131
Size: 3599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 775 1654 2146 2444 555
Size: 220 879 492 298 1710
Fragments arranged by size:
1710 879 492 298 220
BsaI GGTCTCn'nnnn_
Cuts at: 134 3303 134
Size: 3169 430
BsaAI yAC'GTr
Cuts at: 952 952
Size: 3599
BsaBI GATnn'nnATC
Cuts at: 6 1113 3150 6
Size: 1107 2037 455
Fragments arranged by size:
2037 1107 455
BsaHI Gr'CG_yC
Cuts at: 1030 1278 1980 3476 1030
Size: 248 702 1496 1153
Fragments arranged by size:
1496 1153 702 248
BsaWI w'CCGG_w
Cuts at: 788 1309 1987 2261 2408 2819 788
Size: 521 678 274 147 411 1568
Fragments arranged by size:
1568 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 412 412
Size: 3599
BseYI C'CCAG_C
Cuts at: 1024 1782 2359 2855 1024
Size: 758 577 496 1768
Fragments arranged by size:
1768 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 266
Size: 3599
BsiEI CG_ry'CG
Cuts at: 104 1141 1187 2395 104
Size: 1037 46 1208 1308
Fragments arranged by size:
1308 1208 1037 46
BsiHKAI G_wGCw'C
Cuts at: 422 452 613 1391 1581 2373 422
Size: 30 161 778 190 792 1648
Fragments arranged by size:
1648 792 778 190 161 30
Bsp1286I G_dGCh'C
Cuts at: 422 452 613 1224 1317 1391 1581 1643 2373
Size: 30 161 611 93 74 190 62 730
Cuts at: 2373 422
Size: 1648
Fragments arranged by size:
1648 730 611 190 161 93 74 62
30
BspEI T'CCGG_A
Cuts at: 2819 2819
Size: 3599
BspHI T'CATG_A
Cuts at: 2775 2775
Size: 3599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 649 863 1165 1546 649
Size: 214 302 381 2702
Fragments arranged by size:
2702 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1891 1945 3429 3594 50
Size: 1841 54 1484 165 55
Fragments arranged by size:
1841 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1511 1511
Size: 3599
BsrFI r'CCGG_y
Cuts at: 1597 1778 1987 1597
Size: 181 209 3209
Fragments arranged by size:
3209 209 181
BssSI C'ACGA_G
Cuts at: 1870 2228 1870
Size: 358 3241
BstAPI GCAn_nnn'nTGC
Cuts at: 417 523 976 417
Size: 106 453 3040
Fragments arranged by size:
3040 453 106
BstBI TT'CG_AA
Cuts at: 1960 1960
Size: 3599
BstYI r'GATC_y
Cuts at: 1 624 1091 1114 1449 1695 2707 2793 2805
Size: 623 467 23 335 246 1012 86 12
Cuts at: 2805 3151 1
Size: 346 449
Fragments arranged by size:
1012 623 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 209 495 1549 1730 1893 209
Size: 286 1054 181 163 1915
Fragments arranged by size:
1915 1054 286 181 163
BtsI GCAGTG_nn'
Cuts at: 492 842 492
Size: 350 3249
DraI TTT'AAA
Cuts at: 598 598
Size: 3599
DraIII CAC_nnn'GTG
Cuts at: 456 456
Size: 3599
DrdI GACnn_nn'nnGTC
Cuts at: 1305 2163 1305
Size: 858 2741
EaeI y'GGCC_r
Cuts at: 311 1184 1358 1749 1776 3121 311
Size: 873 174 391 27 1345 789
Fragments arranged by size:
1345 873 789 391 174 27
EagI C'GGCC_G
Cuts at: 1184 1184
Size: 3599
EarI CTCTTCn'nnn_
Cuts at: 1622 1832 1622
Size: 210 3389
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 252 409 1807 2117 2263 3409 252
Size: 157 1398 310 146 1146 442
Fragments arranged by size:
1398 1146 442 310 157 146
EcoICRI GAG'CTC
Cuts at: 611 611
Size: 3599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 1424 1856 2603 2871 158
Size: 1266 432 747 268 886
Fragments arranged by size:
1266 886 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 738 738
Size: 3599
EcoRI G'AATT_C
Cuts at: 603 603
Size: 3599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1618 1618
Size: 3599
FspI TGC'GCA
Cuts at: 1380 1380
Size: 3599
HaeII r_GCGC'y
Cuts at: 1033 1281 2303 3282 1033
Size: 248 1022 979 1350
Fragments arranged by size:
1350 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 343 375 491 523 1441 1471 1473 1503 343
Size: 32 116 32 918 30 2 30 2439
Fragments arranged by size:
2439 918 116 32 32 30 30 2
HincII GTy'rAC
Cuts at: 336 638 336
Size: 302 3297
KasI G'GCGC_C
Cuts at: 1029 1277 1029
Size: 248 3351
KpnI G_GTAC'C
Cuts at: 619 619
Size: 3599
MluI A'CGCG_T
Cuts at: 3032 3032
Size: 3599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2270 2454 2270
Size: 184 3415
MscI TGG'CCA
Cuts at: 1360 1360
Size: 3599
MslI CAynn'nnrTG
Cuts at: 1588 1715 1588
Size: 127 3472
MspA1I CmG'CkG
Cuts at: 366 390 710 1024 1384 2397 2642 366
Size: 24 320 314 360 1013 245 1323
Fragments arranged by size:
1323 1013 360 320 314 245 24
NaeI GCC'GGC
Cuts at: 1780 1780
Size: 3599
NarI GG'CG_CC
Cuts at: 1030 1278 1030
Size: 248 3351
NgoMIV G'CCGG_C
Cuts at: 1778 1778
Size: 3599
NspI r_CATG'y
Cuts at: 652 1152 2059 652
Size: 500 907 2192
Fragments arranged by size:
2192 907 500
PciI A'CATG_T
Cuts at: 2055 2055
Size: 3599
PmeI GTTT'AAAC
Cuts at: 598 598
Size: 3599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2770 2738
Size: 32 3567
PstI C_TGCA'G
Cuts at: 376 646 1331 376
Size: 270 685 2644
Fragments arranged by size:
2644 685 270
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3599
PvuII CAG'CTG
Cuts at: 1024 1384 1024
Size: 360 3239
RsrII CG'GwC_CG
Cuts at: 1794 1794
Size: 3599
SacI G_AGCT'C
Cuts at: 613 613
Size: 3599
SalI G'TCGA_C
Cuts at: 636 636
Size: 3599
SapI GCTCTTCn'nnn_
Cuts at: 1622 1832 1622
Size: 210 3389
SbfI CC_TGCA'GG
Cuts at: 646 646
Size: 3599
ScaI AGT'ACT
Cuts at: 358 3116 358
Size: 2758 841
SexAI A'CCwGG_T
Cuts at: 329 491 329
Size: 162 3437
SfcI C'TryA_G
Cuts at: 33 168 372 642 1327 2320 2511 33
Size: 135 204 270 685 993 191 1121
Fragments arranged by size:
1121 993 685 270 204 191 135
SfoI GGC'GCC
Cuts at: 1031 1279 1031
Size: 248 3351
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3599
SmaI CCC'GGG
Cuts at: 621 621
Size: 3599
SmlI C'TyrA_G
Cuts at: 570 754 1669 2161 2423 570
Size: 184 915 492 262 1746
Fragments arranged by size:
1746 915 492 262 184
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3599
SphI G_CATG'C
Cuts at: 652 1152 652
Size: 500 3099
StyI C'CwwG_G
Cuts at: 733 1087 733
Size: 354 3245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1182 1315 1774 1983 1182
Size: 133 459 209 2798
Fragments arranged by size:
2798 459 209 133
TatI w'GTAC_w
Cuts at: 356 3114 356
Size: 2758 841
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3567
Tth111I GACn'n_nGTC
Cuts at: 493 1396 493
Size: 903 2696
XbaI T'CTAG_A
Cuts at: 630 630
Size: 3599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1094 1094
Size: 3599
XmaI C'CCGG_G
Cuts at: 619 619
Size: 3599
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI