Restriction map for
pF1KE0970
(Circular) (Six-base) MAPSORT of: pF1KE0970.seq Check: 4346 from: 1 to: 4682
pF1KE0970 4682 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AatII G_ACGT'C
Cuts at: 959 959
Size: 4682
AccI GT'mk_AC
Cuts at: 1720 1720
Size: 4682
Acc65I G'GTAC_C
Cuts at: 1698 1698
Size: 4682
AclI AA'CG_TT
Cuts at: 4522 4522
Size: 4682
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 472 1521 2507 2939 3686 3954 298
Size: 174 1049 986 432 747 268 1026
Fragments arranged by size:
1049 1026 986 747 432 268 174
AfeI AGC'GCT
Cuts at: 1499 1499
Size: 4682
AflIII A'CryG_T
Cuts at: 1475 2034 3138 4115 1475
Size: 559 1104 977 2042
Fragments arranged by size:
2042 1104 977 559
AgeI A'CCGG_T
Cuts at: 3070 3070
Size: 4682
AlwNI CAG_nnn'CTG
Cuts at: 405 1164 1209 1392 1987 3554 405
Size: 759 45 183 595 1567 1533
Fragments arranged by size:
1567 1533 759 595 183 45
ApaLI G'TGCA_C
Cuts at: 328 1021 3452 328
Size: 693 2431 1558
Fragments arranged by size:
2431 1558 693
ApoI r'AATT_y
Cuts at: 1129 1686 4035 4291 1129
Size: 557 2349 256 1520
Fragments arranged by size:
2349 1520 557 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4682
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4682
AvaI C'yCGr_G
Cuts at: 1702 1702
Size: 4682
BamHI G'GATC_C
Cuts at: 1707 1707
Size: 4682
BanI G'GyrC_C
Cuts at: 379 1444 1698 2112 2360 2395 379
Size: 1065 254 414 248 35 2666
Fragments arranged by size:
2666 1065 414 254 248 35
BanII G_rGCy'C
Cuts at: 362 414 660 667 1601 1696 2726 362
Size: 52 246 7 934 95 1030 2318
Fragments arranged by size:
2318 1030 934 246 95 52 7
BbeI G_GCGC'C
Cuts at: 2116 2364 2116
Size: 248 4434
BbsI GAAGACnn'nnnn_
Cuts at: 1411 1411
Size: 4682
BciVI GTATCCnnnnn_n'
Cuts at: 2580 3341 4682 2580
Size: 761 1341 2580
Fragments arranged by size:
2580 1341 761
BclI T'GATC_A
Cuts at: 2202 2202
Size: 4682
BglI GCCn_nnn'nGGC
Cuts at: 118 583 980 118
Size: 465 397 3820
Fragments arranged by size:
3820 465 397
BglII A'GATC_T
Cuts at: 319 516 965 1550 2197 4234 319
Size: 197 449 585 647 2037 767
Fragments arranged by size:
2037 767 647 585 449 197
BlpI GC'TnA_GC
Cuts at: 1505 1794 1505
Size: 289 4393
Bme1580I G_kGCm'C
Cuts at: 332 382 1025 1447 2307 2400 3456 332
Size: 50 643 422 860 93 1056 1558
Fragments arranged by size:
1558 1056 860 643 422 93 50
BmrI ACTGGGnnnn_n'
Cuts at: 873 1219 1894 1975 2310 4454 873
Size: 346 675 81 335 2144 1101
Fragments arranged by size:
2144 1101 675 346 335 81
BmtI G_CTAG'C
Cuts at: 1504 1504
Size: 4682
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3049 3049
Size: 4682
Bpu10I CC'TnA_GC
Cuts at: 826 826
Size: 4682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 1858 2737 3229 3527 109
Size: 1749 879 492 298 1264
Fragments arranged by size:
1749 1264 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4386 4386
Size: 4682
BsaAI yAC'GTr
Cuts at: 1377 2035 1377
Size: 658 4024
BsaBI GATnn'nnATC
Cuts at: 6 2196 4233 6
Size: 2190 2037 455
Fragments arranged by size:
2190 2037 455
BsaHI Gr'CG_yC
Cuts at: 348 956 2113 2361 3063 4559 348
Size: 608 1157 248 702 1496 471
Fragments arranged by size:
1496 1157 702 608 471 248
BsaWI w'CCGG_w
Cuts at: 1871 2392 3070 3344 3491 3902 1871
Size: 521 678 274 147 411 2651
Fragments arranged by size:
2651 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 316 448 1357 316
Size: 132 909 3641
Fragments arranged by size:
3641 909 132
BseYI C'CCAG_C
Cuts at: 2107 2865 3442 3938 2107
Size: 758 577 496 2851
Fragments arranged by size:
2851 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 445
Size: 4682
BsiEI CG_ry'CG
Cuts at: 104 1261 2224 2270 3478 104
Size: 1157 963 46 1208 1308
Fragments arranged by size:
1308 1208 1157 963 46
BsiHKAI G_wGCw'C
Cuts at: 332 362 667 695 1025 1511 1601 1696 2474
Size: 30 305 28 330 486 90 95 778
Cuts at: 2474 2664 3456 332
Size: 190 792 1558
Fragments arranged by size:
1558 792 778 486 330 305 190 95
90 30 28
BsmI GAATG_Cn'
Cuts at: 807 807
Size: 4682
BsmBI CGTCTCn'nnnn_
Cuts at: 355 355
Size: 4682
Bsp1286I G_dGCh'C
Cuts at: 332 362 382 414 660 667 695 1025 1447
Size: 30 20 32 246 7 28 330 422
Cuts at: 1447 1511 1601 1696 2307 2400 2474 2664 2726
Size: 64 90 95 611 93 74 190 62
Cuts at: 2726 3456 332
Size: 730 1558
Fragments arranged by size:
1558 730 611 422 330 246 190 95
93 90 74 64 62 32 30 28
20 7
BspEI T'CCGG_A
Cuts at: 3902 3902
Size: 4682
BspHI T'CATG_A
Cuts at: 3858 3858
Size: 4682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 773 906 1424 1732 1946 2248 2629 773
Size: 133 518 308 214 302 381 2826
Fragments arranged by size:
2826 518 381 308 302 214 133
BsrBI CCG'CTC
Cuts at: 50 882 2974 3028 4512 4677 50
Size: 832 2092 54 1484 165 55
Fragments arranged by size:
2092 1484 832 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1050 2594 1050
Size: 1544 3138
BsrFI r'CCGG_y
Cuts at: 2680 2861 3070 2680
Size: 181 209 4292
Fragments arranged by size:
4292 209 181
BssSI C'ACGA_G
Cuts at: 2953 3311 2953
Size: 358 4324
BstAPI GCAn_nnn'nTGC
Cuts at: 2059 2059
Size: 4682
BstBI TT'CG_AA
Cuts at: 3043 3043
Size: 4682
BstYI r'GATC_y
Cuts at: 1 319 494 516 530 965 1221 1550 1707
Size: 318 175 22 14 435 256 329 157
Cuts at: 1707 2174 2197 2532 2778 3790 3876 3888 4234
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4234 1
Size: 449
Fragments arranged by size:
1012 467 449 435 346 335 329 318
256 246 175 157 86 23 22 14
12
BtgI C'CryG_G
Cuts at: 107 875 1101 107
Size: 768 226 3688
Fragments arranged by size:
3688 768 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2632 2813 2976 2632
Size: 181 163 4338
Fragments arranged by size:
4338 181 163
BtsI GCAGTG_nn'
Cuts at: 608 1023 1301 1925 608
Size: 415 278 624 3365
Fragments arranged by size:
3365 624 415 278
DraI TTT'AAA
Cuts at: 1681 1681
Size: 4682
DrdI GACnn_nn'nnGTC
Cuts at: 2388 3246 2388
Size: 858 3824
EaeI y'GGCC_r
Cuts at: 110 626 1337 2267 2441 2832 2859 4204 110
Size: 516 711 930 174 391 27 1345 588
Fragments arranged by size:
1345 930 711 588 516 391 174 27
EagI C'GGCC_G
Cuts at: 2267 2267
Size: 4682
EarI CTCTTCn'nnn_
Cuts at: 274 1177 2705 2915 274
Size: 903 1528 210 2041
Fragments arranged by size:
2041 1528 903 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 487 633 1096 1123 2890 3200 3346 4492 487
Size: 146 463 27 1767 310 146 1146 677
Fragments arranged by size:
1767 1146 677 463 310 146 146 27
EcoICRI GAG'CTC
Cuts at: 360 665 1599 1694 360
Size: 305 934 95 3348
Fragments arranged by size:
3348 934 305 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 472 1521 2507 2939 3686 3954 298
Size: 174 1049 986 432 747 268 1026
Fragments arranged by size:
1049 1026 986 747 432 268 174
EcoNI CCTnn'n_nnAGG
Cuts at: 586 685 586
Size: 99 4583
EcoO109I rG'GnC_Cy
Cuts at: 849 867 885 1544 1821 849
Size: 18 18 659 277 3710
Fragments arranged by size:
3710 659 277 18 18
EcoRI G'AATT_C
Cuts at: 1129 1686 1129
Size: 557 4125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4682
FspI TGC'GCA
Cuts at: 2463 2463
Size: 4682
HaeII r_GCGC'y
Cuts at: 215 1460 1501 2116 2364 3386 4365 215
Size: 1245 41 615 248 1022 979 532
Fragments arranged by size:
1245 1022 979 615 532 248 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 799 831 1347 1379 2524 2554 2556 2586 799
Size: 32 516 32 1145 30 2 30 2895
Fragments arranged by size:
2895 1145 516 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1275 1721 1275
Size: 446 4236
HindIII A'AGCT_T
Cuts at: 127 127
Size: 4682
KasI G'GCGC_C
Cuts at: 2112 2360 2112
Size: 248 4434
KpnI G_GTAC'C
Cuts at: 1702 1702
Size: 4682
MluI A'CGCG_T
Cuts at: 4115 4115
Size: 4682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1076 3353 3537 1076
Size: 2277 184 2221
Fragments arranged by size:
2277 2221 184
MscI TGG'CCA
Cuts at: 112 2443 112
Size: 2331 2351
MslI CAynn'nnrTG
Cuts at: 391 2671 2798 391
Size: 2280 127 2275
Fragments arranged by size:
2280 2275 127
MspA1I CmG'CkG
Cuts at: 315 714 1793 2107 2467 3480 3725 315
Size: 399 1079 314 360 1013 245 1272
Fragments arranged by size:
1272 1079 1013 399 360 314 245
NaeI GCC'GGC
Cuts at: 2863 2863
Size: 4682
NarI GG'CG_CC
Cuts at: 2113 2361 2113
Size: 248 4434
NcoI C'CATG_G
Cuts at: 107 1101 107
Size: 994 3688
NgoMIV G'CCGG_C
Cuts at: 2861 2861
Size: 4682
NheI G'CTAG_C
Cuts at: 1500 1500
Size: 4682
NspI r_CATG'y
Cuts at: 489 1248 1479 1735 2235 3142 489
Size: 759 231 256 500 907 2029
Fragments arranged by size:
2029 907 759 500 256 231
PasI CC'CwG_GG
Cuts at: 1440 1440
Size: 4682
PciI A'CATG_T
Cuts at: 1475 3138 1475
Size: 1663 3019
PflMI CCAn_nnn'nTGG
Cuts at: 405 1209 405
Size: 804 3878
PmeI GTTT'AAAC
Cuts at: 1681 1681
Size: 4682
PmlI CAC'GTG
Cuts at: 1377 1377
Size: 4682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 3821
Size: 32 4650
PpuMI rG'GwC_Cy
Cuts at: 849 867 885 1544 849
Size: 18 18 659 3987
Fragments arranged by size:
3987 659 18 18
PshAI GACnn'nnGTC
Cuts at: 247 247
Size: 4682
PstI C_TGCA'G
Cuts at: 1729 2414 1729
Size: 685 3997
PvuI CG_AT'CG
Cuts at: 104 1261 104
Size: 1157 3525
PvuII CAG'CTG
Cuts at: 315 714 2107 2467 315
Size: 399 1393 360 2530
Fragments arranged by size:
2530 1393 399 360
RsrII CG'GwC_CG
Cuts at: 2877 2877
Size: 4682
SacI G_AGCT'C
Cuts at: 362 667 1601 1696 362
Size: 305 934 95 3348
Fragments arranged by size:
3348 934 305 95
SalI G'TCGA_C
Cuts at: 1719 1719
Size: 4682
SapI GCTCTTCn'nnn_
Cuts at: 2705 2915 2705
Size: 210 4472
SbfI CC_TGCA'GG
Cuts at: 1729 1729
Size: 4682
ScaI AGT'ACT
Cuts at: 190 307 4199 190
Size: 117 3892 673
Fragments arranged by size:
3892 673 117
SfcI C'TryA_G
Cuts at: 33 177 1725 2410 3403 3594 33
Size: 144 1548 685 993 191 1121
Fragments arranged by size:
1548 1121 993 685 191 144
SfiI GGCCn_nnn'nGGCC
Cuts at: 583 583
Size: 4682
SfoI GGC'GCC
Cuts at: 2114 2362 2114
Size: 248 4434
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4682
SmaI CCC'GGG
Cuts at: 1704 1704
Size: 4682
SmlI C'TyrA_G
Cuts at: 124 1837 2752 3244 3506 124
Size: 1713 915 492 262 1300
Fragments arranged by size:
1713 1300 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4682
SphI G_CATG'C
Cuts at: 489 1248 1735 2235 489
Size: 759 487 500 2936
Fragments arranged by size:
2936 759 500 487
StuI AGG'CCT
Cuts at: 1567 1567
Size: 4682
StyI C'CwwG_G
Cuts at: 107 1101 1816 2170 107
Size: 994 715 354 2619
Fragments arranged by size:
2619 994 715 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 659 1260 2265 2398 2857 3066 659
Size: 601 1005 133 459 209 2275
Fragments arranged by size:
2275 1005 601 459 209 133
TatI w'GTAC_w
Cuts at: 188 305 437 1047 1196 4197 188
Size: 117 132 610 149 3001 673
Fragments arranged by size:
3001 673 610 149 132 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4199 4231 4199
Size: 32 4650
Tth111I GACn'n_nGTC
Cuts at: 1270 1636 2479 1270
Size: 366 843 3473
Fragments arranged by size:
3473 843 366
XbaI T'CTAG_A
Cuts at: 1713 1713
Size: 4682
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2177 2177
Size: 4682
XmaI C'CCGG_G
Cuts at: 1702 1702
Size: 4682
ZraI GAC'GTC
Cuts at: 957 957
Size: 4682
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BpmI BsaXI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NotI NruI NsiI PacI PfoI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI