Restriction map for
pF1KE0969
(Circular) (Six-base) MAPSORT of: pF1KE0969.seq Check: 4301 from: 1 to: 4694
pF1KE0969 4694 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1107 1732 1107
Size: 625 4069
Acc65I G'GTAC_C
Cuts at: 557 1710 557
Size: 1153 3541
AclI AA'CG_TT
Cuts at: 4534 4534
Size: 4694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 1049 1433 1539 1568 1649 2519 2951 3698
Size: 678 384 106 29 81 870 432 747
Cuts at: 3698 3966 371
Size: 268 1099
Fragments arranged by size:
1099 870 747 678 432 384 268 106
81 29
AflII C'TTAA_G
Cuts at: 414 414
Size: 4694
AflIII A'CryG_T
Cuts at: 2046 3150 4127 2046
Size: 1104 977 2613
Fragments arranged by size:
2613 1104 977
AgeI A'CCGG_T
Cuts at: 3082 3082
Size: 4694
AlwNI CAG_nnn'CTG
Cuts at: 1613 1999 3566 1613
Size: 386 1567 2741
Fragments arranged by size:
2741 1567 386
ApaLI G'TGCA_C
Cuts at: 3464 3464
Size: 4694
ApoI r'AATT_y
Cuts at: 1322 1521 1698 4047 4303 1322
Size: 199 177 2349 256 1713
Fragments arranged by size:
2349 1713 256 199 177
AseI AT'TA_AT
Cuts at: 20 20
Size: 4694
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
AvaI C'yCGr_G
Cuts at: 1714 1714
Size: 4694
BamHI G'GATC_C
Cuts at: 652 1719 652
Size: 1067 3627
BanI G'GyrC_C
Cuts at: 557 1578 1710 2124 2372 2407 557
Size: 1021 132 414 248 35 2844
Fragments arranged by size:
2844 1021 414 248 132 35
BanII G_rGCy'C
Cuts at: 1708 2738 1708
Size: 1030 3664
BbeI G_GCGC'C
Cuts at: 2128 2376 2128
Size: 248 4446
BciVI GTATCCnnnnn_n'
Cuts at: 2592 3353 4694 2592
Size: 761 1341 2592
Fragments arranged by size:
2592 1341 761
BclI T'GATC_A
Cuts at: 2214 2214
Size: 4694
BfrBI ATG'CAT
Cuts at: 1313 1313
Size: 4694
BglII A'GATC_T
Cuts at: 2209 4246 2209
Size: 2037 2657
BlpI GC'TnA_GC
Cuts at: 256 1806 256
Size: 1550 3144
Bme1580I G_kGCm'C
Cuts at: 2319 2412 3468 2319
Size: 93 1056 3545
Fragments arranged by size:
3545 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1906 1987 2322 4466 1906
Size: 81 335 2144 2134
Fragments arranged by size:
2144 2134 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3061 3061
Size: 4694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1270 463
Size: 807 3887
Bpu10I CC'TnA_GC
Cuts at: 202 1192 202
Size: 990 3704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1870 2749 3241 3539 1870
Size: 879 492 298 3025
Fragments arranged by size:
3025 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 470 823 4398 470
Size: 353 3575 766
Fragments arranged by size:
3575 766 353
BsaAI yAC'GTr
Cuts at: 2047 2047
Size: 4694
BsaBI GATnn'nnATC
Cuts at: 6 2208 4245 6
Size: 2202 2037 455
Fragments arranged by size:
2202 2037 455
BsaHI Gr'CG_yC
Cuts at: 2125 2373 3075 4571 2125
Size: 248 702 1496 2248
Fragments arranged by size:
2248 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1883 2404 3082 3356 3503 3914 1883
Size: 521 678 274 147 411 2663
Fragments arranged by size:
2663 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 236 877 2119 2877 3454 3950 236
Size: 641 1242 758 577 496 980
Fragments arranged by size:
1242 980 758 641 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 319 190
Size: 129 4565
BsiEI CG_ry'CG
Cuts at: 104 2236 2282 3490 104
Size: 2132 46 1208 1308
Fragments arranged by size:
2132 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 208 1708 2486 2676 3468 208
Size: 1500 778 190 792 1434
Fragments arranged by size:
1500 1434 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 835 835
Size: 4694
Bsp1286I G_dGCh'C
Cuts at: 208 1708 2319 2412 2486 2676 2738 3468 208
Size: 1500 611 93 74 190 62 730 1434
Fragments arranged by size:
1500 1434 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3914 3914
Size: 4694
BspHI T'CATG_A
Cuts at: 821 3870 821
Size: 3049 1645
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1744 1958 2260 2641 1744
Size: 214 302 381 3797
Fragments arranged by size:
3797 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2986 3040 4524 4689 50
Size: 2936 54 1484 165 55
Fragments arranged by size:
2936 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2606 2606
Size: 4694
BsrFI r'CCGG_y
Cuts at: 814 2692 2873 3082 814
Size: 1878 181 209 2426
Fragments arranged by size:
2426 1878 209 181
BsrGI T'GTAC_A
Cuts at: 1604 1604
Size: 4694
BssSI C'ACGA_G
Cuts at: 2965 3323 2965
Size: 358 4336
BstAPI GCAn_nnn'nTGC
Cuts at: 2071 2071
Size: 4694
BstBI TT'CG_AA
Cuts at: 3055 3055
Size: 4694
BstXI CCAn_nnnn'nTGG
Cuts at: 1400 1400
Size: 4694
BstYI r'GATC_y
Cuts at: 1 359 652 906 1719 2186 2209 2544 2790
Size: 358 293 254 813 467 23 335 246
Cuts at: 2790 3802 3888 3900 4246 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 813 467 449 358 346 335 293
254 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1108 1108
Size: 4694
BtgI C'CryG_G
Cuts at: 250 250
Size: 4694
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 226 2644 2825 2988 226
Size: 2418 181 163 1932
Fragments arranged by size:
2418 1932 181 163
BtsI GCAGTG_nn'
Cuts at: 319 1937 319
Size: 1618 3076
DraI TTT'AAA
Cuts at: 1693 1693
Size: 4694
DrdI GACnn_nn'nnGTC
Cuts at: 2400 3258 2400
Size: 858 3836
EaeI y'GGCC_r
Cuts at: 2279 2453 2844 2871 4216 2279
Size: 174 391 27 1345 2757
Fragments arranged by size:
2757 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2279 2279
Size: 4694
EarI CTCTTCn'nnn_
Cuts at: 209 347 1025 1040 2717 2927 209
Size: 138 678 15 1677 210 1976
Fragments arranged by size:
1976 1677 678 210 138 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2902 3212 3358 4504 2902
Size: 310 146 1146 3092
Fragments arranged by size:
3092 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1706 1706
Size: 4694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 463 1049 1270 1433 1539 1568 1649 2519
Size: 92 586 221 163 106 29 81 870
Cuts at: 2519 2951 3698 3966 371
Size: 432 747 268 1099
Fragments arranged by size:
1099 870 747 586 432 268 221 163
106 92 81 29
EcoO109I rG'GnC_Cy
Cuts at: 1833 1833
Size: 4694
EcoRI G'AATT_C
Cuts at: 1698 1698
Size: 4694
EcoRV GAT'ATC
Cuts at: 838 838
Size: 4694
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1207 1438 2713 1207
Size: 231 1275 3188
Fragments arranged by size:
3188 1275 231
FspI TGC'GCA
Cuts at: 2475 2475
Size: 4694
HaeII r_GCGC'y
Cuts at: 2128 2376 3398 4377 2128
Size: 248 1022 979 2445
Fragments arranged by size:
2445 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1441 1473 2536 2566 2568 2598 1441
Size: 32 1063 30 2 30 3537
Fragments arranged by size:
3537 1063 32 30 30 2
HincII GTy'rAC
Cuts at: 1733 1733
Size: 4694
HindIII A'AGCT_T
Cuts at: 1189 1189
Size: 4694
KasI G'GCGC_C
Cuts at: 2124 2372 2124
Size: 248 4446
KpnI G_GTAC'C
Cuts at: 561 1714 561
Size: 1153 3541
MfeI C'AATT_G
Cuts at: 262 262
Size: 4694
MluI A'CGCG_T
Cuts at: 4127 4127
Size: 4694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 308 3365 3549 308
Size: 3057 184 1453
Fragments arranged by size:
3057 1453 184
MscI TGG'CCA
Cuts at: 2455 2455
Size: 4694
MslI CAynn'nnrTG
Cuts at: 968 2683 2810 968
Size: 1715 127 2852
Fragments arranged by size:
2852 1715 127
MspA1I CmG'CkG
Cuts at: 877 1113 1610 1805 2119 2479 3492 3737 877
Size: 236 497 195 314 360 1013 245 1834
Fragments arranged by size:
1834 1013 497 360 314 245 236 195
NaeI GCC'GGC
Cuts at: 2875 2875
Size: 4694
NarI GG'CG_CC
Cuts at: 2125 2373 2125
Size: 248 4446
NgoMIV G'CCGG_C
Cuts at: 2873 2873
Size: 4694
NsiI A_TGCA'T
Cuts at: 1315 1315
Size: 4694
NspI r_CATG'y
Cuts at: 1313 1747 2247 3154 1313
Size: 434 500 907 2853
Fragments arranged by size:
2853 907 500 434
PciI A'CATG_T
Cuts at: 3150 3150
Size: 4694
PmeI GTTT'AAAC
Cuts at: 1693 1693
Size: 4694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 3833
Size: 32 4662
PshAI GACnn'nnGTC
Cuts at: 606 606
Size: 4694
PsiI TTA'TAA
Cuts at: 928 928
Size: 4694
PstI C_TGCA'G
Cuts at: 1741 2426 1741
Size: 685 4009
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4694
PvuII CAG'CTG
Cuts at: 877 1610 2119 2479 877
Size: 733 509 360 3092
Fragments arranged by size:
3092 733 509 360
RsrII CG'GwC_CG
Cuts at: 2889 2889
Size: 4694
SacI G_AGCT'C
Cuts at: 1708 1708
Size: 4694
SalI G'TCGA_C
Cuts at: 1731 1731
Size: 4694
SapI GCTCTTCn'nnn_
Cuts at: 209 2717 2927 209
Size: 2508 210 1976
Fragments arranged by size:
2508 1976 210
SbfI CC_TGCA'GG
Cuts at: 1741 1741
Size: 4694
ScaI AGT'ACT
Cuts at: 4211 4211
Size: 4694
SfcI C'TryA_G
Cuts at: 33 1737 2422 3415 3606 33
Size: 1704 685 993 191 1121
Fragments arranged by size:
1704 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2126 2374 2126
Size: 248 4446
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
SmaI CCC'GGG
Cuts at: 1716 1716
Size: 4694
SmlI C'TyrA_G
Cuts at: 414 1849 2764 3256 3518 414
Size: 1435 915 492 262 1590
Fragments arranged by size:
1590 1435 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4694
SphI G_CATG'C
Cuts at: 1747 2247 1747
Size: 500 4194
StyI C'CwwG_G
Cuts at: 1828 2182 1828
Size: 354 4340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 250 2277 2410 2869 3078 250
Size: 2027 133 459 209 1866
Fragments arranged by size:
2027 1866 459 209 133
TatI w'GTAC_w
Cuts at: 1433 1604 4209 1433
Size: 171 2605 1918
Fragments arranged by size:
2605 1918 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 4662
Tth111I GACn'n_nGTC
Cuts at: 2491 2491
Size: 4694
XbaI T'CTAG_A
Cuts at: 1725 1725
Size: 4694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2189
Size: 4694
XmaI C'CCGG_G
Cuts at: 1714 1714
Size: 4694
XmnI GAAnn'nnTTC
Cuts at: 218 1418 218
Size: 1200 3494
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BsaXI BseRI BsiWI BsmI BssHII BstEII Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI NcoI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI