Restriction map for
pF1KE0965
(Circular) (Six-base) MAPSORT of: pF1KE0965.seq Check: 4538 from: 1 to: 4640
pF1KE0965 4640 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 302 1678 302
Size: 1376 3264
Acc65I G'GTAC_C
Cuts at: 1656 1656
Size: 4640
AclI AA'CG_TT
Cuts at: 4480 4480
Size: 4640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 976 2465 2897 3644 3912 893
Size: 83 1489 432 747 268 1621
Fragments arranged by size:
1621 1489 747 432 268 83
AfeI AGC'GCT
Cuts at: 1246 1246
Size: 4640
AflIII A'CryG_T
Cuts at: 206 1992 3096 4073 206
Size: 1786 1104 977 773
Fragments arranged by size:
1786 1104 977 773
AgeI A'CCGG_T
Cuts at: 3028 3028
Size: 4640
AlwNI CAG_nnn'CTG
Cuts at: 1945 3512 1945
Size: 1567 3073
ApaLI G'TGCA_C
Cuts at: 3410 3410
Size: 4640
ApoI r'AATT_y
Cuts at: 462 550 1644 3993 4249 462
Size: 88 1094 2349 256 853
Fragments arranged by size:
2349 1094 853 256 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 4640
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
AvaI C'yCGr_G
Cuts at: 584 1660 584
Size: 1076 3564
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 918 951 918
Size: 33 4607
BamHI G'GATC_C
Cuts at: 1665 1665
Size: 4640
BanI G'GyrC_C
Cuts at: 1237 1656 2070 2318 2353 1237
Size: 419 414 248 35 3524
Fragments arranged by size:
3524 419 414 248 35
BanII G_rGCy'C
Cuts at: 617 1654 2684 617
Size: 1037 1030 2573
Fragments arranged by size:
2573 1037 1030
BbeI G_GCGC'C
Cuts at: 2074 2322 2074
Size: 248 4392
BbsI GAAGACnn'nnnn_
Cuts at: 685 685
Size: 4640
BbvCI CC'TCA_GC
Cuts at: 877 877
Size: 4640
BciVI GTATCCnnnnn_n'
Cuts at: 1002 2538 3299 4640 1002
Size: 1536 761 1341 1002
Fragments arranged by size:
1536 1341 1002 761
BclI T'GATC_A
Cuts at: 2160 2160
Size: 4640
BfrBI ATG'CAT
Cuts at: 1188 1188
Size: 4640
BglII A'GATC_T
Cuts at: 812 2155 4192 812
Size: 1343 2037 1260
Fragments arranged by size:
2037 1343 1260
BlpI GC'TnA_GC
Cuts at: 1752 1752
Size: 4640
Bme1580I G_kGCm'C
Cuts at: 1240 2265 2358 3414 1240
Size: 1025 93 1056 2466
Fragments arranged by size:
2466 1056 1025 93
BmgBI CAC'GTC
Cuts at: 300 591 300
Size: 291 4349
BmrI ACTGGGnnnn_n'
Cuts at: 1852 1933 2268 4412 1852
Size: 81 335 2144 2080
Fragments arranged by size:
2144 2080 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 559 3007 559
Size: 2448 2192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 915 915
Size: 4640
Bpu10I CC'TnA_GC
Cuts at: 877 877
Size: 4640
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1816 2695 3187 3485 1816
Size: 879 492 298 2971
Fragments arranged by size:
2971 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 229 4344 229
Size: 4115 525
BsaAI yAC'GTr
Cuts at: 1993 1993
Size: 4640
BsaBI GATnn'nnATC
Cuts at: 6 2154 4191 6
Size: 2148 2037 455
Fragments arranged by size:
2148 2037 455
BsaHI Gr'CG_yC
Cuts at: 2071 2319 3021 4517 2071
Size: 248 702 1496 2194
Fragments arranged by size:
2194 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1829 2350 3028 3302 3449 3860 1829
Size: 521 678 274 147 411 2609
Fragments arranged by size:
2609 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 810 810
Size: 4640
BseYI C'CCAG_C
Cuts at: 422 531 938 2065 2823 3400 3896 422
Size: 109 407 1127 758 577 496 1166
Fragments arranged by size:
1166 1127 758 577 496 407 109
BsiEI CG_ry'CG
Cuts at: 104 2182 2228 3436 104
Size: 2078 46 1208 1308
Fragments arranged by size:
2078 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1201 1654 2432 2622 3414 1201
Size: 453 778 190 792 2427
Fragments arranged by size:
2427 792 778 453 190
BsmI GAATG_Cn'
Cuts at: 1188 1540 1188
Size: 352 4288
BsmBI CGTCTCn'nnnn_
Cuts at: 581 581
Size: 4640
Bsp1286I G_dGCh'C
Cuts at: 617 1201 1240 1654 2265 2358 2432 2622 2684
Size: 584 39 414 611 93 74 190 62
Cuts at: 2684 3414 617
Size: 730 1843
Fragments arranged by size:
1843 730 611 584 414 190 93 74
62 39
BspEI T'CCGG_A
Cuts at: 3860 3860
Size: 4640
BspHI T'CATG_A
Cuts at: 143 963 3816 143
Size: 820 2853 967
Fragments arranged by size:
2853 967 820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 749 1690 1904 2206 2587 749
Size: 941 214 302 381 2802
Fragments arranged by size:
2802 941 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2932 2986 4470 4635 50
Size: 2882 54 1484 165 55
Fragments arranged by size:
2882 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 902 1100 1374 2552 902
Size: 198 274 1178 2990
Fragments arranged by size:
2990 1178 274 198
BsrFI r'CCGG_y
Cuts at: 2638 2819 3028 2638
Size: 181 209 4250
Fragments arranged by size:
4250 209 181
BsrGI T'GTAC_A
Cuts at: 670 860 670
Size: 190 4450
BssSI C'ACGA_G
Cuts at: 2911 3269 2911
Size: 358 4282
BstAPI GCAn_nnn'nTGC
Cuts at: 2017 2017
Size: 4640
BstBI TT'CG_AA
Cuts at: 1026 3001 1026
Size: 1975 2665
BstXI CCAn_nnnn'nTGG
Cuts at: 1284 1359 1284
Size: 75 4565
BstYI r'GATC_y
Cuts at: 1 812 1665 2132 2155 2490 2736 3748 3834
Size: 811 853 467 23 335 246 1012 86
Cuts at: 3834 3846 4192 1
Size: 12 346 449
Fragments arranged by size:
1012 853 811 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 377 107
Size: 270 4370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2590 2771 2934 2590
Size: 181 163 4296
Fragments arranged by size:
4296 181 163
BtsI GCAGTG_nn'
Cuts at: 268 1570 1883 268
Size: 1302 313 3025
Fragments arranged by size:
3025 1302 313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 612 647 612
Size: 35 4605
DraI TTT'AAA
Cuts at: 555 1118 1639 555
Size: 563 521 3556
Fragments arranged by size:
3556 563 521
DrdI GACnn_nn'nnGTC
Cuts at: 360 2346 3204 360
Size: 1986 858 1796
Fragments arranged by size:
1986 1796 858
EaeI y'GGCC_r
Cuts at: 369 1349 1517 2225 2399 2790 2817 4162 369
Size: 980 168 708 174 391 27 1345 847
Fragments arranged by size:
1345 980 847 708 391 174 168 27
EagI C'GGCC_G
Cuts at: 2225 2225
Size: 4640
EarI CTCTTCn'nnn_
Cuts at: 2663 2873 2663
Size: 210 4430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1371 2848 3158 3304 4450 1371
Size: 1477 310 146 1146 1561
Fragments arranged by size:
1561 1477 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1652 1652
Size: 4640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 915 976 2465 2897 3644 3912 893
Size: 22 61 1489 432 747 268 1621
Fragments arranged by size:
1621 1489 747 432 268 61 22
EcoO109I rG'GnC_Cy
Cuts at: 1082 1779 1082
Size: 697 3943
EcoRI G'AATT_C
Cuts at: 1644 1644
Size: 4640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2659 2659
Size: 4640
FspI TGC'GCA
Cuts at: 2421 2421
Size: 4640
HaeII r_GCGC'y
Cuts at: 1248 2074 2322 3344 4323 1248
Size: 826 248 1022 979 1565
Fragments arranged by size:
1565 1022 979 826 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 820 852 904 936 1188 1220 2482 2512 2514
Size: 32 52 32 252 32 1262 30 2
Cuts at: 2514 2544 820
Size: 30 2916
Fragments arranged by size:
2916 1262 252 52 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 192 1679 192
Size: 1487 3153
KasI G'GCGC_C
Cuts at: 2070 2318 2070
Size: 248 4392
KpnI G_GTAC'C
Cuts at: 1660 1660
Size: 4640
MluI A'CGCG_T
Cuts at: 4073 4073
Size: 4640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 612 719 3311 3495 612
Size: 107 2592 184 1757
Fragments arranged by size:
2592 1757 184 107
MscI TGG'CCA
Cuts at: 371 1351 1519 2401 371
Size: 980 168 882 2610
Fragments arranged by size:
2610 980 882 168
MslI CAynn'nnrTG
Cuts at: 211 871 962 1282 2629 2756 211
Size: 660 91 320 1347 127 2095
Fragments arranged by size:
2095 1347 660 320 127 91
MspA1I CmG'CkG
Cuts at: 426 785 942 1751 2065 2425 3438 3683 426
Size: 359 157 809 314 360 1013 245 1383
Fragments arranged by size:
1383 1013 809 360 359 314 245 157
NaeI GCC'GGC
Cuts at: 2821 2821
Size: 4640
NarI GG'CG_CC
Cuts at: 2071 2319 2071
Size: 248 4392
NcoI C'CATG_G
Cuts at: 107 107
Size: 4640
NgoMIV G'CCGG_C
Cuts at: 2819 2819
Size: 4640
NsiI A_TGCA'T
Cuts at: 1190 1190
Size: 4640
NspI r_CATG'y
Cuts at: 210 1693 2193 3100 210
Size: 1483 500 907 1750
Fragments arranged by size:
1750 1483 907 500
PciI A'CATG_T
Cuts at: 206 3096 206
Size: 2890 1750
PflMI CCAn_nnn'nTGG
Cuts at: 378 1487 378
Size: 1109 3531
PmeI GTTT'AAAC
Cuts at: 1639 1639
Size: 4640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 705 737 3779 3811 705
Size: 32 3042 32 1534
Fragments arranged by size:
3042 1534 32 32
PpuMI rG'GwC_Cy
Cuts at: 1082 1082
Size: 4640
PsiI TTA'TAA
Cuts at: 1304 1304
Size: 4640
PstI C_TGCA'G
Cuts at: 1687 2372 1687
Size: 685 3955
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4640
PvuII CAG'CTG
Cuts at: 426 785 942 2065 2425 426
Size: 359 157 1123 360 2641
Fragments arranged by size:
2641 1123 360 359 157
RsrII CG'GwC_CG
Cuts at: 2835 2835
Size: 4640
SacI G_AGCT'C
Cuts at: 1654 1654
Size: 4640
SalI G'TCGA_C
Cuts at: 1677 1677
Size: 4640
SanDI GG'GwC_CC
Cuts at: 1082 1082
Size: 4640
SapI GCTCTTCn'nnn_
Cuts at: 2663 2873 2663
Size: 210 4430
SbfI CC_TGCA'GG
Cuts at: 1687 1687
Size: 4640
ScaI AGT'ACT
Cuts at: 4157 4157
Size: 4640
SfcI C'TryA_G
Cuts at: 33 497 1683 2368 3361 3552 33
Size: 464 1186 685 993 191 1121
Fragments arranged by size:
1186 1121 993 685 464 191
SfoI GGC'GCC
Cuts at: 2072 2320 2072
Size: 248 4392
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
SmaI CCC'GGG
Cuts at: 1662 1662
Size: 4640
SmlI C'TyrA_G
Cuts at: 1795 2710 3202 3464 1795
Size: 915 492 262 2971
Fragments arranged by size:
2971 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4640
SphI G_CATG'C
Cuts at: 1693 2193 1693
Size: 500 4140
SspI AAT'ATT
Cuts at: 1131 1131
Size: 4640
StyI C'CwwG_G
Cuts at: 107 185 390 1086 1774 2128 107
Size: 78 205 696 688 354 2619
Fragments arranged by size:
2619 696 688 354 205 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2223 2356 2815 3024 2223
Size: 133 459 209 3839
Fragments arranged by size:
3839 459 209 133
TatI w'GTAC_w
Cuts at: 670 860 4155 670
Size: 190 3295 1155
Fragments arranged by size:
3295 1155 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 449 481 652 684 4157 4189 449
Size: 32 171 32 3473 32 900
Fragments arranged by size:
3473 900 171 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2437 2437
Size: 4640
XbaI T'CTAG_A
Cuts at: 1266 1671 1266
Size: 405 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 475 1248 2135 475
Size: 773 887 2980
Fragments arranged by size:
2980 887 773
XmaI C'CCGG_G
Cuts at: 1660 1660
Size: 4640
XmnI GAAnn'nnTTC
Cuts at: 814 814
Size: 4640
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BcgI BglI BmtI BsaXI BsgI BsiWI
BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PshAI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI