Restriction map for
pF1KE0947
(Circular) (Six-base) MAPSORT of: pF1KE0947.seq Check: 1692 from: 1 to: 4268
pF1KE0947 4268 bp
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AccI GT'mk_AC
Cuts at: 1306 1306
Size: 4268
Acc65I G'GTAC_C
Cuts at: 1284 1284
Size: 4268
AclI AA'CG_TT
Cuts at: 4108 4108
Size: 4268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2093 2525 3272 3540 2093
Size: 432 747 268 2821
Fragments arranged by size:
2821 747 432 268
AfeI AGC'GCT
Cuts at: 152 152
Size: 4268
AflII C'TTAA_G
Cuts at: 1227 1227
Size: 4268
AflIII A'CryG_T
Cuts at: 839 1620 2724 3701 839
Size: 781 1104 977 1406
Fragments arranged by size:
1406 1104 977 781
AgeI A'CCGG_T
Cuts at: 2656 2656
Size: 4268
AlwNI CAG_nnn'CTG
Cuts at: 1050 1573 3140 1050
Size: 523 1567 2178
Fragments arranged by size:
2178 1567 523
ApaI G_GGCC'C
Cuts at: 215 215
Size: 4268
ApaLI G'TGCA_C
Cuts at: 3038 3038
Size: 4268
ApoI r'AATT_y
Cuts at: 704 1272 3621 3877 704
Size: 568 2349 256 1095
Fragments arranged by size:
2349 1095 568 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
AvaI C'yCGr_G
Cuts at: 138 214 278 1288 138
Size: 76 64 1010 3118
Fragments arranged by size:
3118 1010 76 64
BamHI G'GATC_C
Cuts at: 565 593 1293 565
Size: 28 700 3540
Fragments arranged by size:
3540 700 28
BanI G'GyrC_C
Cuts at: 118 1284 1698 1946 1981 118
Size: 1166 414 248 35 2405
Fragments arranged by size:
2405 1166 414 248 35
BanII G_rGCy'C
Cuts at: 215 542 849 1282 2312 215
Size: 327 307 433 1030 2171
Fragments arranged by size:
2171 1030 433 327 307
BbeI G_GCGC'C
Cuts at: 122 1702 1950 122
Size: 1580 248 2440
Fragments arranged by size:
2440 1580 248
BbsI GAAGACnn'nnnn_
Cuts at: 361 772 1079 1166 361
Size: 411 307 87 3463
Fragments arranged by size:
3463 411 307 87
BciVI GTATCCnnnnn_n'
Cuts at: 236 2166 2927 4268 236
Size: 1930 761 1341 236
Fragments arranged by size:
1930 1341 761 236
BclI T'GATC_A
Cuts at: 857 1067 1788 857
Size: 210 721 3337
Fragments arranged by size:
3337 721 210
BglI GCCn_nnn'nGGC
Cuts at: 232 232
Size: 4268
BglII A'GATC_T
Cuts at: 482 1783 3820 482
Size: 1301 2037 930
Fragments arranged by size:
2037 1301 930
BlpI GC'TnA_GC
Cuts at: 433 1380 433
Size: 947 3321
Bme1580I G_kGCm'C
Cuts at: 215 1893 1986 3042 215
Size: 1678 93 1056 1441
Fragments arranged by size:
1678 1441 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1480 1561 1896 4040 1480
Size: 81 335 2144 1708
Fragments arranged by size:
2144 1708 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 556 1157 2635 556
Size: 601 1478 2189
Fragments arranged by size:
2189 1478 601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 1087 583
Size: 504 3764
Bpu10I CC'TnA_GC
Cuts at: 560 811 560
Size: 251 4017
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 2323 2815 3113 1444
Size: 879 492 298 2599
Fragments arranged by size:
2599 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3972 3972
Size: 4268
BsaAI yAC'GTr
Cuts at: 1621 1621
Size: 4268
BsaBI GATnn'nnATC
Cuts at: 6 1782 3819 6
Size: 1776 2037 455
Fragments arranged by size:
2037 1776 455
BsaHI Gr'CG_yC
Cuts at: 119 191 1699 1947 2649 4145 119
Size: 72 1508 248 702 1496 242
Fragments arranged by size:
1508 1496 702 248 242 72
BsaWI w'CCGG_w
Cuts at: 529 1457 1978 2656 2930 3077 3488 529
Size: 928 521 678 274 147 411 1309
Fragments arranged by size:
1309 928 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1693 2451 3028 3524 1693
Size: 758 577 496 2437
Fragments arranged by size:
2437 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 1151 851
Size: 300 3968
BsiEI CG_ry'CG
Cuts at: 104 1810 1856 3064 104
Size: 1706 46 1208 1308
Fragments arranged by size:
1706 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 542 1282 2060 2250 3042 542
Size: 740 778 190 792 1768
Fragments arranged by size:
1768 792 778 740 190
Bsp1286I G_dGCh'C
Cuts at: 215 542 849 1282 1893 1986 2060 2250 2312
Size: 327 307 433 611 93 74 190 62
Cuts at: 2312 3042 215
Size: 730 1441
Fragments arranged by size:
1441 730 611 433 327 307 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3488 3488
Size: 4268
BspHI T'CATG_A
Cuts at: 3444 3444
Size: 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1318 1532 1834 2215 1318
Size: 214 302 381 3371
Fragments arranged by size:
3371 381 302 214
BsrBI CCG'CTC
Cuts at: 50 579 2560 2614 4098 4263 50
Size: 529 1981 54 1484 165 55
Fragments arranged by size:
1981 1484 529 165 55 54
BsrDI GCAATG_nn'
Cuts at: 554 2180 554
Size: 1626 2642
BsrFI r'CCGG_y
Cuts at: 187 2266 2447 2656 187
Size: 2079 181 209 1799
Fragments arranged by size:
2079 1799 209 181
BssSI C'ACGA_G
Cuts at: 2539 2897 2539
Size: 358 3910
BstAPI GCAn_nnn'nTGC
Cuts at: 636 1645 636
Size: 1009 3259
BstBI TT'CG_AA
Cuts at: 2629 2629
Size: 4268
BstXI CCAn_nnnn'nTGG
Cuts at: 834 834
Size: 4268
BstYI r'GATC_y
Cuts at: 1 482 565 593 1162 1293 1760 1783 2118
Size: 481 83 28 569 131 467 23 335
Cuts at: 2118 2364 3376 3462 3474 3820 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 569 481 467 449 346 335 246
131 86 83 28 23 12
Bsu36I CC'TnA_GG
Cuts at: 800 800
Size: 4268
BtgI C'CryG_G
Cuts at: 107 1073 107
Size: 966 3302
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1012 2218 2399 2562 1012
Size: 1206 181 163 2718
Fragments arranged by size:
2718 1206 181 163
BtsI GCAGTG_nn'
Cuts at: 839 1511 839
Size: 672 3596
DraI TTT'AAA
Cuts at: 1267 1267
Size: 4268
DraIII CAC_nnn'GTG
Cuts at: 775 775
Size: 4268
DrdI GACnn_nn'nnGTC
Cuts at: 280 1974 2832 280
Size: 1694 858 1716
Fragments arranged by size:
1716 1694 858
EaeI y'GGCC_r
Cuts at: 964 1041 1853 2027 2418 2445 3790 964
Size: 77 812 174 391 27 1345 1442
Fragments arranged by size:
1442 1345 812 391 174 77 27
EagI C'GGCC_G
Cuts at: 1853 1853
Size: 4268
EarI CTCTTCn'nnn_
Cuts at: 689 2291 2501 689
Size: 1602 210 2456
Fragments arranged by size:
2456 1602 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2476 2786 2932 4078 2476
Size: 310 146 1146 2666
Fragments arranged by size:
2666 1146 310 146
EcoICRI GAG'CTC
Cuts at: 540 1280 540
Size: 740 3528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 1087 2093 2525 3272 3540 583
Size: 504 1006 432 747 268 1311
Fragments arranged by size:
1311 1006 747 504 432 268
EcoO109I rG'GnC_Cy
Cuts at: 205 282 779 1047 1136 1407 205
Size: 77 497 268 89 271 3066
Fragments arranged by size:
3066 497 271 268 89 77
EcoRI G'AATT_C
Cuts at: 1272 1272
Size: 4268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 836 1242 2287 836
Size: 406 1045 2817
Fragments arranged by size:
2817 1045 406
FspI TGC'GCA
Cuts at: 2049 2049
Size: 4268
HaeII r_GCGC'y
Cuts at: 122 154 1702 1950 2972 3951 122
Size: 32 1548 248 1022 979 439
Fragments arranged by size:
1548 1022 979 439 248 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 284 316 483 515 2110 2140 2142 2172 284
Size: 32 167 32 1595 30 2 30 2380
Fragments arranged by size:
2380 1595 167 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1307 1307
Size: 4268
KasI G'GCGC_C
Cuts at: 118 1698 1946 118
Size: 1580 248 2440
Fragments arranged by size:
2440 1580 248
KpnI G_GTAC'C
Cuts at: 1288 1288
Size: 4268
MluI A'CGCG_T
Cuts at: 3701 3701
Size: 4268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 174 2939 3123 174
Size: 2765 184 1319
Fragments arranged by size:
2765 1319 184
MscI TGG'CCA
Cuts at: 966 1043 2029 966
Size: 77 986 3205
Fragments arranged by size:
3205 986 77
MslI CAynn'nnrTG
Cuts at: 514 689 2257 2384 514
Size: 175 1568 127 2398
Fragments arranged by size:
2398 1568 175 127
MspA1I CmG'CkG
Cuts at: 1039 1379 1693 2053 3066 3311 1039
Size: 340 314 360 1013 245 1996
Fragments arranged by size:
1996 1013 360 340 314 245
NaeI GCC'GGC
Cuts at: 189 2449 189
Size: 2260 2008
NarI GG'CG_CC
Cuts at: 119 1699 1947 119
Size: 1580 248 2440
Fragments arranged by size:
2440 1580 248
NcoI C'CATG_G
Cuts at: 107 1073 107
Size: 966 3302
NdeI CA'TA_TG
Cuts at: 864 1208 864
Size: 344 3924
NgoMIV G'CCGG_C
Cuts at: 187 2447 187
Size: 2260 2008
NspI r_CATG'y
Cuts at: 634 843 1035 1321 1821 2728 634
Size: 209 192 286 500 907 2174
Fragments arranged by size:
2174 907 500 286 209 192
PasI CC'CwG_GG
Cuts at: 1061 1061
Size: 4268
PciI A'CATG_T
Cuts at: 839 2724 839
Size: 1885 2383
PflMI CCAn_nnn'nTGG
Cuts at: 477 1050 477
Size: 573 3695
PfoI T'CCnGG_A
Cuts at: 736 1158 736
Size: 422 3846
PmeI GTTT'AAAC
Cuts at: 1267 1267
Size: 4268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 4236
PpuMI rG'GwC_Cy
Cuts at: 282 779 282
Size: 497 3771
PspOMI G'GGCC_C
Cuts at: 211 211
Size: 4268
PstI C_TGCA'G
Cuts at: 641 1083 1315 2000 641
Size: 442 232 685 2909
Fragments arranged by size:
2909 685 442 232
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4268
PvuII CAG'CTG
Cuts at: 1039 1693 2053 1039
Size: 654 360 3254
Fragments arranged by size:
3254 654 360
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 4268
SacI G_AGCT'C
Cuts at: 542 1282 542
Size: 740 3528
SalI G'TCGA_C
Cuts at: 1305 1305
Size: 4268
SanDI GG'GwC_CC
Cuts at: 779 779
Size: 4268
SapI GCTCTTCn'nnn_
Cuts at: 2291 2501 2291
Size: 210 4058
SbfI CC_TGCA'GG
Cuts at: 1315 1315
Size: 4268
ScaI AGT'ACT
Cuts at: 3785 3785
Size: 4268
SfcI C'TryA_G
Cuts at: 33 637 1079 1311 1996 2989 3180 33
Size: 604 442 232 685 993 191 1121
Fragments arranged by size:
1121 993 685 604 442 232 191
SfoI GGC'GCC
Cuts at: 120 1700 1948 120
Size: 1580 248 2440
Fragments arranged by size:
2440 1580 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
SmaI CCC'GGG
Cuts at: 140 280 1290 140
Size: 140 1010 3118
Fragments arranged by size:
3118 1010 140
SmlI C'TyrA_G
Cuts at: 1227 1423 2338 2830 3092 1227
Size: 196 915 492 262 2403
Fragments arranged by size:
2403 915 492 262 196
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4268
SphI G_CATG'C
Cuts at: 634 1321 1821 634
Size: 687 500 3081
Fragments arranged by size:
3081 687 500
StyI C'CwwG_G
Cuts at: 107 961 1073 1402 1756 107
Size: 854 112 329 354 2619
Fragments arranged by size:
2619 854 354 329 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1053 1851 1984 2443 2652 1053
Size: 798 133 459 209 2669
Fragments arranged by size:
2669 798 459 209 133
TatI w'GTAC_w
Cuts at: 788 3783 788
Size: 2995 1273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 732 764 3785 3817 732
Size: 32 3021 32 1183
Fragments arranged by size:
3021 1183 32 32
Tth111I GACn'n_nGTC
Cuts at: 2065 2065
Size: 4268
XbaI T'CTAG_A
Cuts at: 1299 1299
Size: 4268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 129 155 1763 129
Size: 26 1608 2634
Fragments arranged by size:
2634 1608 26
XmaI C'CCGG_G
Cuts at: 138 278 1288 138
Size: 140 1010 3118
Fragments arranged by size:
3118 1010 140
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI