Restriction map for
pF1KE0934
(Circular) (Six-base) MAPSORT of: pF1KE0934.seq Check: 3597 from: 1 to: 4361
pF1KE0934 4361 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1191 1191
Size: 4361
AccI GT'mk_AC
Cuts at: 1399 1399
Size: 4361
Acc65I G'GTAC_C
Cuts at: 1377 1377
Size: 4361
AclI AA'CG_TT
Cuts at: 4201 4201
Size: 4361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1292 2186 2618 3365 3633 692
Size: 600 894 432 747 268 1420
Fragments arranged by size:
1420 894 747 600 432 268
AflIII A'CryG_T
Cuts at: 1713 2817 3794 1713
Size: 1104 977 2280
Fragments arranged by size:
2280 1104 977
AgeI A'CCGG_T
Cuts at: 2749 2749
Size: 4361
AhdI GACnn_n'nnGTC
Cuts at: 714 714
Size: 4361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 272 272
Size: 4361
AlwNI CAG_nnn'CTG
Cuts at: 1666 3233 1666
Size: 1567 2794
ApaLI G'TGCA_C
Cuts at: 3131 3131
Size: 4361
ApoI r'AATT_y
Cuts at: 488 527 922 936 1324 1365 3714 3970 488
Size: 39 395 14 388 41 2349 256 879
Fragments arranged by size:
2349 879 395 388 256 41 39 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4361
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
AvaI C'yCGr_G
Cuts at: 1249 1381 1249
Size: 132 4229
BamHI G'GATC_C
Cuts at: 1386 1386
Size: 4361
BanI G'GyrC_C
Cuts at: 1377 1791 2039 2074 1377
Size: 414 248 35 3664
Fragments arranged by size:
3664 414 248 35
BanII G_rGCy'C
Cuts at: 1033 1375 2405 1033
Size: 342 1030 2989
Fragments arranged by size:
2989 1030 342
BbeI G_GCGC'C
Cuts at: 1795 2043 1795
Size: 248 4113
BbsI GAAGACnn'nnnn_
Cuts at: 308 952 308
Size: 644 3717
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3020 4361 2259
Size: 761 1341 2259
Fragments arranged by size:
2259 1341 761
BclI T'GATC_A
Cuts at: 1881 1881
Size: 4361
BfrBI ATG'CAT
Cuts at: 774 774
Size: 4361
BglII A'GATC_T
Cuts at: 1140 1876 3913 1140
Size: 736 2037 1588
Fragments arranged by size:
2037 1588 736
BlpI GC'TnA_GC
Cuts at: 1473 1473
Size: 4361
Bme1580I G_kGCm'C
Cuts at: 1986 2079 3135 1986
Size: 93 1056 3212
Fragments arranged by size:
3212 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1573 1654 1989 4133 1573
Size: 81 335 2144 1801
Fragments arranged by size:
2144 1801 335 81
BmtI G_CTAG'C
Cuts at: 1226 1226
Size: 4361
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 4361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 348 1537 2416 2908 3206 298
Size: 50 1189 879 492 298 1453
Fragments arranged by size:
1453 1189 879 492 298 50
BsaI GGTCTCn'nnnn_
Cuts at: 4065 4065
Size: 4361
BsaAI yAC'GTr
Cuts at: 1714 1714
Size: 4361
BsaBI GATnn'nnATC
Cuts at: 6 773 1875 3912 6
Size: 767 1102 2037 455
Fragments arranged by size:
2037 1102 767 455
BsaHI Gr'CG_yC
Cuts at: 1792 2040 2742 4238 1792
Size: 248 702 1496 1915
Fragments arranged by size:
1915 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1550 2071 2749 3023 3170 3581 1550
Size: 521 678 274 147 411 2330
Fragments arranged by size:
2330 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 245 1786 2544 3121 3617 245
Size: 1541 758 577 496 989
Fragments arranged by size:
1541 989 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1903 1949 3157 104
Size: 1799 46 1208 1308
Fragments arranged by size:
1799 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1375 2153 2343 3135 1375
Size: 778 190 792 2601
Fragments arranged by size:
2601 792 778 190
BsiWI C'GTAC_G
Cuts at: 1092 1092
Size: 4361
BsmI GAATG_Cn'
Cuts at: 513 513
Size: 4361
Bsp1286I G_dGCh'C
Cuts at: 1033 1375 1986 2079 2153 2343 2405 3135 1033
Size: 342 611 93 74 190 62 730 2259
Fragments arranged by size:
2259 730 611 342 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3581 3581
Size: 4361
BspHI T'CATG_A
Cuts at: 3537 3537
Size: 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1191 1411 1625 1927 2308 1191
Size: 220 214 302 381 3244
Fragments arranged by size:
3244 381 302 220 214
BsrBI CCG'CTC
Cuts at: 50 2653 2707 4191 4356 50
Size: 2603 54 1484 165 55
Fragments arranged by size:
2603 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2273 2273
Size: 4361
BsrFI r'CCGG_y
Cuts at: 120 2359 2540 2749 120
Size: 2239 181 209 1732
Fragments arranged by size:
2239 1732 209 181
BssSI C'ACGA_G
Cuts at: 2632 2990 2632
Size: 358 4003
BstAPI GCAn_nnn'nTGC
Cuts at: 1738 1738
Size: 4361
BstBI TT'CG_AA
Cuts at: 2722 2722
Size: 4361
BstXI CCAn_nnnn'nTGG
Cuts at: 139 139
Size: 4361
BstYI r'GATC_y
Cuts at: 1 432 879 1140 1386 1853 1876 2211 2457
Size: 431 447 261 246 467 23 335 246
Cuts at: 2457 3469 3555 3567 3913 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 447 431 346 335 261
246 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4361
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2311 2492 2655 2311
Size: 181 163 4017
Fragments arranged by size:
4017 181 163
BtsI GCAGTG_nn'
Cuts at: 1604 1604
Size: 4361
DraI TTT'AAA
Cuts at: 377 494 1360 377
Size: 117 866 3378
Fragments arranged by size:
3378 866 117
DrdI GACnn_nn'nnGTC
Cuts at: 2067 2925 2067
Size: 858 3503
EaeI y'GGCC_r
Cuts at: 1946 2120 2511 2538 3883 1946
Size: 174 391 27 1345 2424
Fragments arranged by size:
2424 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1946 1946
Size: 4361
EarI CTCTTCn'nnn_
Cuts at: 2384 2594 2384
Size: 210 4151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2569 2879 3025 4171 2569
Size: 310 146 1146 2759
Fragments arranged by size:
2759 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1373 1373
Size: 4361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1292 2186 2618 3365 3633 692
Size: 600 894 432 747 268 1420
Fragments arranged by size:
1420 894 747 600 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1500 1500
Size: 4361
EcoRI G'AATT_C
Cuts at: 1365 1365
Size: 4361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4361
FspI TGC'GCA
Cuts at: 2142 2142
Size: 4361
HaeII r_GCGC'y
Cuts at: 1795 2043 3065 4044 1795
Size: 248 1022 979 2112
Fragments arranged by size:
2112 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 700 732 939 971 1000 1027 1032 1059 2203
Size: 32 207 32 29 27 5 27 1144
Cuts at: 2203 2233 2235 2265 700
Size: 30 2 30 2796
Fragments arranged by size:
2796 1144 207 32 32 30 30 29
27 27 5 2
HincII GTy'rAC
Cuts at: 1400 1400
Size: 4361
KasI G'GCGC_C
Cuts at: 1791 2039 1791
Size: 248 4113
KpnI G_GTAC'C
Cuts at: 1381 1381
Size: 4361
MluI A'CGCG_T
Cuts at: 3794 3794
Size: 4361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 807 841 3032 3216 807
Size: 34 2191 184 1952
Fragments arranged by size:
2191 1952 184 34
MscI TGG'CCA
Cuts at: 2122 2122
Size: 4361
MslI CAynn'nnrTG
Cuts at: 1007 2350 2477 1007
Size: 1343 127 2891
Fragments arranged by size:
2891 1343 127
MspA1I CmG'CkG
Cuts at: 612 1472 1786 2146 3159 3404 612
Size: 860 314 360 1013 245 1569
Fragments arranged by size:
1569 1013 860 360 314 245
NaeI GCC'GGC
Cuts at: 2542 2542
Size: 4361
NarI GG'CG_CC
Cuts at: 1792 2040 1792
Size: 248 4113
NcoI C'CATG_G
Cuts at: 107 107
Size: 4361
NdeI CA'TA_TG
Cuts at: 348 348
Size: 4361
NgoMIV G'CCGG_C
Cuts at: 2540 2540
Size: 4361
NheI G'CTAG_C
Cuts at: 1222 1222
Size: 4361
NsiI A_TGCA'T
Cuts at: 776 776
Size: 4361
NspI r_CATG'y
Cuts at: 1414 1914 2821 1414
Size: 500 907 2954
Fragments arranged by size:
2954 907 500
PciI A'CATG_T
Cuts at: 2817 2817
Size: 4361
PmeI GTTT'AAAC
Cuts at: 1360 1360
Size: 4361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 4329
PstI C_TGCA'G
Cuts at: 1408 2093 1408
Size: 685 3676
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4361
PvuII CAG'CTG
Cuts at: 612 1786 2146 612
Size: 1174 360 2827
Fragments arranged by size:
2827 1174 360
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 4361
SacI G_AGCT'C
Cuts at: 1375 1375
Size: 4361
SalI G'TCGA_C
Cuts at: 1398 1398
Size: 4361
SapI GCTCTTCn'nnn_
Cuts at: 2384 2594 2384
Size: 210 4151
SbfI CC_TGCA'GG
Cuts at: 1408 1408
Size: 4361
ScaI AGT'ACT
Cuts at: 3878 3878
Size: 4361
SfcI C'TryA_G
Cuts at: 33 896 1404 2089 3082 3273 33
Size: 863 508 685 993 191 1121
Fragments arranged by size:
1121 993 863 685 508 191
SfoI GGC'GCC
Cuts at: 1793 2041 1793
Size: 248 4113
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
SgrAI Cr'CCGG_yG
Cuts at: 120 120
Size: 4361
SmaI CCC'GGG
Cuts at: 1383 1383
Size: 4361
SmlI C'TyrA_G
Cuts at: 277 327 1249 1516 2431 2923 3185 277
Size: 50 922 267 915 492 262 1453
Fragments arranged by size:
1453 922 915 492 267 262 50
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4361
SphI G_CATG'C
Cuts at: 1414 1914 1414
Size: 500 3861
StyI C'CwwG_G
Cuts at: 107 746 1495 1849 107
Size: 639 749 354 2619
Fragments arranged by size:
2619 749 639 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1944 2077 2536 2745 1944
Size: 133 459 209 3560
Fragments arranged by size:
3560 459 209 133
TatI w'GTAC_w
Cuts at: 1119 3876 1119
Size: 2757 1604
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 4329
Tth111I GACn'n_nGTC
Cuts at: 2158 2158
Size: 4361
XbaI T'CTAG_A
Cuts at: 1392 1392
Size: 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1856 1856
Size: 4361
XhoI C'TCGA_G
Cuts at: 1249 1249
Size: 4361
XmaI C'CCGG_G
Cuts at: 1381 1381
Size: 4361
XmnI GAAnn'nnTTC
Cuts at: 305 677 1325 305
Size: 372 648 3341
Fragments arranged by size:
3341 648 372
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NsiI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BpmI Bpu10I BsaXI
BseRI BsgI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
ZraI