Restriction map for
pF1KE0902
(Circular) (Six-base) MAPSORT of: pF1KE0902.seq Check: 2558 from: 1 to: 3566
pF1KE0902 3566 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 604 604
Size: 3566
Acc65I G'GTAC_C
Cuts at: 582 582
Size: 3566
AclI AA'CG_TT
Cuts at: 3406 3406
Size: 3566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1391 1823 2570 2838 1391
Size: 432 747 268 2119
Fragments arranged by size:
2119 747 432 268
AflIII A'CryG_T
Cuts at: 918 2022 2999 918
Size: 1104 977 1485
Fragments arranged by size:
1485 1104 977
AgeI A'CCGG_T
Cuts at: 1954 1954
Size: 3566
AleI CACnn'nnGTG
Cuts at: 130 130
Size: 3566
AlwNI CAG_nnn'CTG
Cuts at: 871 2438 871
Size: 1567 1999
ApaI G_GGCC'C
Cuts at: 409 409
Size: 3566
ApaLI G'TGCA_C
Cuts at: 2336 2336
Size: 3566
ApoI r'AATT_y
Cuts at: 231 570 2919 3175 231
Size: 339 2349 256 622
Fragments arranged by size:
2349 622 339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3566
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
AvaI C'yCGr_G
Cuts at: 397 586 397
Size: 189 3377
AvrII C'CTAG_G
Cuts at: 165 165
Size: 3566
BamHI G'GATC_C
Cuts at: 591 591
Size: 3566
BanI G'GyrC_C
Cuts at: 345 391 582 996 1244 1279 345
Size: 46 191 414 248 35 2632
Fragments arranged by size:
2632 414 248 191 46 35
BanII G_rGCy'C
Cuts at: 115 409 528 580 1610 115
Size: 294 119 52 1030 2071
Fragments arranged by size:
2071 1030 294 119 52
BbeI G_GCGC'C
Cuts at: 1000 1248 1000
Size: 248 3318
BbvCI CC'TCA_GC
Cuts at: 451 451
Size: 3566
BciVI GTATCCnnnnn_n'
Cuts at: 1464 2225 3566 1464
Size: 761 1341 1464
Fragments arranged by size:
1464 1341 761
BclI T'GATC_A
Cuts at: 1086 1086
Size: 3566
BglII A'GATC_T
Cuts at: 1081 3118 1081
Size: 2037 1529
BlpI GC'TnA_GC
Cuts at: 678 678
Size: 3566
Bme1580I G_kGCm'C
Cuts at: 284 396 409 1191 1284 2340 284
Size: 112 13 782 93 1056 1510
Fragments arranged by size:
1510 1056 782 112 93 13
BmrI ACTGGGnnnn_n'
Cuts at: 547 778 859 1194 3338 547
Size: 231 81 335 2144 775
Fragments arranged by size:
2144 775 335 231 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 412 1933 412
Size: 1521 2045
Bpu10I CC'TnA_GC
Cuts at: 451 451
Size: 3566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1621 2113 2411 742
Size: 879 492 298 1897
Fragments arranged by size:
1897 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3270 3270
Size: 3566
BsaAI yAC'GTr
Cuts at: 919 919
Size: 3566
BsaBI GATnn'nnATC
Cuts at: 6 1080 3117 6
Size: 1074 2037 455
Fragments arranged by size:
2037 1074 455
BsaHI Gr'CG_yC
Cuts at: 997 1245 1947 3443 997
Size: 248 702 1496 1120
Fragments arranged by size:
1496 1120 702 248
BsaWI w'CCGG_w
Cuts at: 554 755 1276 1954 2228 2375 2786 554
Size: 201 521 678 274 147 411 1334
Fragments arranged by size:
1334 678 521 411 274 201 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 408 408
Size: 3566
BseYI C'CCAG_C
Cuts at: 372 991 1749 2326 2822 372
Size: 619 758 577 496 1116
Fragments arranged by size:
1116 758 619 577 496
BsiEI CG_ry'CG
Cuts at: 104 1108 1154 2362 104
Size: 1004 46 1208 1308
Fragments arranged by size:
1308 1208 1004 46
BsiHKAI G_wGCw'C
Cuts at: 115 296 580 1358 1548 2340 115
Size: 181 284 778 190 792 1341
Fragments arranged by size:
1341 792 778 284 190 181
BsmI GAATG_Cn'
Cuts at: 154 154
Size: 3566
Bsp1286I G_dGCh'C
Cuts at: 115 284 296 396 409 528 580 1191 1284
Size: 169 12 100 13 119 52 611 93
Cuts at: 1284 1358 1548 1610 2340 115
Size: 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 190 169 119 100 93
74 62 52 13 12
BspEI T'CCGG_A
Cuts at: 2786 2786
Size: 3566
BspHI T'CATG_A
Cuts at: 2742 2742
Size: 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 616 830 1132 1513 616
Size: 214 302 381 2669
Fragments arranged by size:
2669 381 302 214
BsrBI CCG'CTC
Cuts at: 50 533 1858 1912 3396 3561 50
Size: 483 1325 54 1484 165 55
Fragments arranged by size:
1484 1325 483 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1478 1478
Size: 3566
BsrFI r'CCGG_y
Cuts at: 1564 1745 1954 1564
Size: 181 209 3176
Fragments arranged by size:
3176 209 181
BsrGI T'GTAC_A
Cuts at: 191 191
Size: 3566
BssSI C'ACGA_G
Cuts at: 1837 2195 1837
Size: 358 3208
BstAPI GCAn_nnn'nTGC
Cuts at: 943 943
Size: 3566
BstBI TT'CG_AA
Cuts at: 1927 1927
Size: 3566
BstXI CCAn_nnnn'nTGG
Cuts at: 385 385
Size: 3566
BstYI r'GATC_y
Cuts at: 1 487 591 1058 1081 1416 1662 2674 2760
Size: 486 104 467 23 335 246 1012 86
Cuts at: 2760 2772 3118 1
Size: 12 346 449
Fragments arranged by size:
1012 486 467 449 346 335 246 104
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 341 341
Size: 3566
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1516 1697 1860 1516
Size: 181 163 3222
Fragments arranged by size:
3222 181 163
BtsI GCAGTG_nn'
Cuts at: 464 809 464
Size: 345 3221
ClaI AT'CG_AT
Cuts at: 426 426
Size: 3566
DraI TTT'AAA
Cuts at: 565 565
Size: 3566
DrdI GACnn_nn'nnGTC
Cuts at: 1272 2130 1272
Size: 858 2708
EaeI y'GGCC_r
Cuts at: 1151 1325 1716 1743 3088 1151
Size: 174 391 27 1345 1629
Fragments arranged by size:
1629 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1151 1151
Size: 3566
EarI CTCTTCn'nnn_
Cuts at: 1589 1799 1589
Size: 210 3356
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1774 2084 2230 3376 1774
Size: 310 146 1146 1964
Fragments arranged by size:
1964 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 578 113
Size: 465 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1391 1823 2570 2838 1391
Size: 432 747 268 2119
Fragments arranged by size:
2119 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 340 340
Size: 3566
EcoO109I rG'GnC_Cy
Cuts at: 405 406 705 405
Size: 1 299 3266
Fragments arranged by size:
3266 299 1
EcoRI G'AATT_C
Cuts at: 570 570
Size: 3566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1585 1585
Size: 3566
FspI TGC'GCA
Cuts at: 1347 1347
Size: 3566
HaeII r_GCGC'y
Cuts at: 460 1000 1248 2270 3249 460
Size: 540 248 1022 979 777
Fragments arranged by size:
1022 979 777 540 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 419 451 1408 1438 1440 1470 419
Size: 32 957 30 2 30 2515
Fragments arranged by size:
2515 957 32 30 30 2
HincII GTy'rAC
Cuts at: 605 605
Size: 3566
KasI G'GCGC_C
Cuts at: 996 1244 996
Size: 248 3318
KpnI G_GTAC'C
Cuts at: 586 586
Size: 3566
MluI A'CGCG_T
Cuts at: 2999 2999
Size: 3566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 230 2237 2421 230
Size: 2007 184 1375
Fragments arranged by size:
2007 1375 184
MscI TGG'CCA
Cuts at: 1327 1327
Size: 3566
MslI CAynn'nnrTG
Cuts at: 130 1555 1682 130
Size: 1425 127 2014
Fragments arranged by size:
2014 1425 127
MspA1I CmG'CkG
Cuts at: 494 677 991 1351 2364 2609 494
Size: 183 314 360 1013 245 1451
Fragments arranged by size:
1451 1013 360 314 245 183
NaeI GCC'GGC
Cuts at: 1747 1747
Size: 3566
NarI GG'CG_CC
Cuts at: 997 1245 997
Size: 248 3318
NgoMIV G'CCGG_C
Cuts at: 1745 1745
Size: 3566
NspI r_CATG'y
Cuts at: 619 1119 2026 619
Size: 500 907 2159
Fragments arranged by size:
2159 907 500
PasI CC'CwG_GG
Cuts at: 410 410
Size: 3566
PciI A'CATG_T
Cuts at: 2022 2022
Size: 3566
PmeI GTTT'AAAC
Cuts at: 565 565
Size: 3566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2705 2737 2705
Size: 32 3534
PspOMI G'GGCC_C
Cuts at: 405 405
Size: 3566
PstI C_TGCA'G
Cuts at: 613 1298 613
Size: 685 2881
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3566
PvuII CAG'CTG
Cuts at: 494 991 1351 494
Size: 497 360 2709
Fragments arranged by size:
2709 497 360
RsrII CG'GwC_CG
Cuts at: 1761 1761
Size: 3566
SacI G_AGCT'C
Cuts at: 115 580 115
Size: 465 3101
SalI G'TCGA_C
Cuts at: 603 603
Size: 3566
SapI GCTCTTCn'nnn_
Cuts at: 1589 1799 1589
Size: 210 3356
SbfI CC_TGCA'GG
Cuts at: 613 613
Size: 3566
ScaI AGT'ACT
Cuts at: 3083 3083
Size: 3566
SfcI C'TryA_G
Cuts at: 33 609 1294 2287 2478 33
Size: 576 685 993 191 1121
Fragments arranged by size:
1121 993 685 576 191
SfoI GGC'GCC
Cuts at: 998 1246 998
Size: 248 3318
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
SmaI CCC'GGG
Cuts at: 588 588
Size: 3566
SmlI C'TyrA_G
Cuts at: 721 1636 2128 2390 721
Size: 915 492 262 1897
Fragments arranged by size:
1897 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3566
SphI G_CATG'C
Cuts at: 619 1119 619
Size: 500 3066
StyI C'CwwG_G
Cuts at: 165 700 1054 165
Size: 535 354 2677
Fragments arranged by size:
2677 535 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1149 1282 1741 1950 1149
Size: 133 459 209 2765
Fragments arranged by size:
2765 459 209 133
TatI w'GTAC_w
Cuts at: 191 3081 191
Size: 2890 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 3534
Tth111I GACn'n_nGTC
Cuts at: 1363 1363
Size: 3566
XbaI T'CTAG_A
Cuts at: 597 597
Size: 3566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1061 1061
Size: 3566
XmaI C'CCGG_G
Cuts at: 586 586
Size: 3566
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BpmI
BsaXI BsgI BsiWI BsmBI BssHII BstEII BstZ17I BtgI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI