Restriction map for
pF1KE0895
(Circular) (Six-base) MAPSORT of: pF1KE0895.seq Check: 2225 from: 1 to: 4052
pF1KE0895 4052 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 835 835
Size: 4052
AccI GT'mk_AC
Cuts at: 1090 1090
Size: 4052
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 470 2440 470
Size: 1970 2082
AleI CACnn'nnGTG
Cuts at: 685 910 685
Size: 225 3827
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaLI G'TGCA_C
Cuts at: 672 2822 672
Size: 2150 1902
ApoI r'AATT_y
Cuts at: 1056 3405 3661 1056
Size: 2349 256 1447
Fragments arranged by size:
2349 1447 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 544 627 1068 1482 1730 1765 544
Size: 83 441 414 248 35 2831
Fragments arranged by size:
2831 441 414 248 83 35
BanII G_rGCy'C
Cuts at: 1008 1066 2096 1008
Size: 58 1030 2964
Fragments arranged by size:
2964 1030 58
BbeI G_GCGC'C
Cuts at: 548 1486 1734 548
Size: 938 248 2866
Fragments arranged by size:
2866 938 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 653 687 653
Size: 34 4018
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BglI GCCn_nnn'nGGC
Cuts at: 460 553 460
Size: 93 3959
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 676 1677 1770 2826 676
Size: 1001 93 1056 1902
Fragments arranged by size:
1902 1056 1001 93
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1002 1228 2107 2599 2897 1002
Size: 226 879 492 298 2157
Fragments arranged by size:
2157 879 492 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 986 3756 986
Size: 2770 1282
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 700 1566 3603 6
Size: 694 866 2037 455
Fragments arranged by size:
2037 866 694 455
BsaHI Gr'CG_yC
Cuts at: 545 878 1483 1731 2433 3929 545
Size: 333 605 248 702 1496 668
Fragments arranged by size:
1496 702 668 605 333 248
BsaWI w'CCGG_w
Cuts at: 470 1241 1762 2440 2714 2861 3272 470
Size: 771 521 678 274 147 411 1250
Fragments arranged by size:
1250 771 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 885 885
Size: 4052
BseYI C'CCAG_C
Cuts at: 1477 2235 2812 3308 1477
Size: 758 577 496 2221
Fragments arranged by size:
2221 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 676 1066 1844 2034 2826 676
Size: 390 778 190 792 1902
Fragments arranged by size:
1902 792 778 390 190
BsmBI CGTCTCn'nnnn_
Cuts at: 172 607 172
Size: 435 3617
Bsp1286I G_dGCh'C
Cuts at: 676 1008 1066 1677 1770 1844 2034 2096 2826
Size: 332 58 611 93 74 190 62 730
Cuts at: 2826 676
Size: 1902
Fragments arranged by size:
1902 730 611 332 190 93 74 62
58
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 512 782 3228 512
Size: 270 2446 1336
Fragments arranged by size:
2446 1336 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 835 1102 1316 1618 1999 242
Size: 593 267 214 302 381 2295
Fragments arranged by size:
2295 593 381 302 267 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1964 1964
Size: 4052
BsrFI r'CCGG_y
Cuts at: 470 2050 2231 2440 470
Size: 1580 181 209 2082
Fragments arranged by size:
2082 1580 209 181
BsrGI T'GTAC_A
Cuts at: 938 938
Size: 4052
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 427 427
Size: 4052
BstYI r'GATC_y
Cuts at: 1 695 1077 1544 1567 1902 2148 3160 3246
Size: 694 382 467 23 335 246 1012 86
Cuts at: 3246 3258 3604 1
Size: 12 346 449
Fragments arranged by size:
1012 694 467 449 382 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4052
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 1295 1295
Size: 4052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 720 755 720
Size: 35 4017
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 248 296 326 479 776 1637 1811 2202 2229
Size: 48 30 153 297 861 174 391 27
Cuts at: 2229 3574 248
Size: 1345 726
Fragments arranged by size:
1345 861 726 391 297 174 153 48
30 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 862 2075 2285 862
Size: 1213 210 2629
Fragments arranged by size:
2629 1213 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1064 1064
Size: 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 789 1191 789
Size: 402 3650
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 834 2071 834
Size: 1237 2815
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 548 1486 1734 2756 3735 548
Size: 938 248 1022 979 865
Fragments arranged by size:
1022 979 938 865 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 585 617 1894 1924 1926 1956 585
Size: 32 1277 30 2 30 2681
Fragments arranged by size:
2681 1277 32 30 30 2
HincII GTy'rAC
Cuts at: 1023 1091 1023
Size: 68 3984
HindIII A'AGCT_T
Cuts at: 923 923
Size: 4052
KasI G'GCGC_C
Cuts at: 544 1482 1730 544
Size: 938 248 2866
Fragments arranged by size:
2866 938 248
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 687 2723 2907 687
Size: 2036 184 1832
Fragments arranged by size:
2036 1832 184
MscI TGG'CCA
Cuts at: 250 298 328 481 778 1813 250
Size: 48 30 153 297 1035 2489
Fragments arranged by size:
2489 1035 297 153 48 30
MslI CAynn'nnrTG
Cuts at: 223 280 685 772 910 955 2041 2168 223
Size: 57 405 87 138 45 1086 127 2107
Fragments arranged by size:
2107 1086 405 138 127 87 57 45
MspA1I CmG'CkG
Cuts at: 1163 1477 1837 2850 3095 1163
Size: 314 360 1013 245 2120
Fragments arranged by size:
2120 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 545 1483 1731 545
Size: 938 248 2866
Fragments arranged by size:
2866 938 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4052
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 210 1105 1605 2512 210
Size: 895 500 907 1750
Fragments arranged by size:
1750 907 895 500
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PpuMI rG'GwC_Cy
Cuts at: 789 789
Size: 4052
PstI C_TGCA'G
Cuts at: 801 832 1099 1784 801
Size: 31 267 685 3069
Fragments arranged by size:
3069 685 267 31
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 1477 1837 1477
Size: 360 3692
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 1066 1066
Size: 4052
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SexAI A'CCwGG_T
Cuts at: 212 212
Size: 4052
SfcI C'TryA_G
Cuts at: 33 797 828 975 1095 1780 2773 2964 33
Size: 764 31 147 120 685 993 191 1121
Fragments arranged by size:
1121 993 764 685 191 147 120 31
SfoI GGC'GCC
Cuts at: 546 1484 1732 546
Size: 938 248 2866
Fragments arranged by size:
2866 938 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 981 1207 2122 2614 2876 981
Size: 226 915 492 262 2157
Fragments arranged by size:
2157 915 492 262 226
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StyI C'CwwG_G
Cuts at: 107 1186 1540 107
Size: 1079 354 2619
Fragments arranged by size:
2619 1079 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 888 1635 1768 2227 2436 888
Size: 747 133 459 209 2504
Fragments arranged by size:
2504 747 459 209 133
TatI w'GTAC_w
Cuts at: 284 410 938 3567 284
Size: 126 528 2629 769
Fragments arranged by size:
2629 769 528 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 442 1849 442
Size: 1407 2645
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 1547 306
Size: 1241 2811
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
XmnI GAAnn'nnTTC
Cuts at: 817 967 817
Size: 150 3902
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI