Restriction map for
pF1KE0894
(Circular) (Six-base) MAPSORT of: pF1KE0894.seq Check: 1680 from: 1 to: 4040
pF1KE0894 4040 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1865 2297 3044 3312 259
Size: 1606 432 747 268 987
Fragments arranged by size:
1606 987 747 432 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AleI CACnn'nnGTG
Cuts at: 530 850 530
Size: 320 3720
AlwNI CAG_nnn'CTG
Cuts at: 1008 1345 2912 1008
Size: 337 1567 2136
Fragments arranged by size:
2136 1567 337
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 1044 3393 3649 1044
Size: 2349 256 1435
Fragments arranged by size:
2349 1435 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 1056 1470 1718 1753 1056
Size: 414 248 35 3343
Fragments arranged by size:
3343 414 248 35
BanII G_rGCy'C
Cuts at: 1054 2084 1054
Size: 1030 3010
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbsI GAAGACnn'nnnn_
Cuts at: 385 385
Size: 4040
BbvCI CC'TCA_GC
Cuts at: 652 652
Size: 4040
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 1665 1758 2814 1665
Size: 93 1056 2891
Fragments arranged by size:
2891 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
Bpu10I CC'TnA_GC
Cuts at: 652 652
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 772 1216 2095 2587 2885 318
Size: 454 444 879 492 298 1473
Fragments arranged by size:
1473 879 492 454 444 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 924 1393 924
Size: 469 3571
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 153 153
Size: 4040
BseYI C'CCAG_C
Cuts at: 394 1465 2223 2800 3296 394
Size: 1071 758 577 496 1138
Fragments arranged by size:
1138 1071 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 724 568
Size: 156 3884
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1054 1832 2022 2814 1054
Size: 778 190 792 2280
Fragments arranged by size:
2280 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 610 610
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 1054 1665 1758 1832 2022 2084 2814 1054
Size: 611 93 74 190 62 730 2280
Fragments arranged by size:
2280 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 3216 3216
Size: 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 245 1090 1304 1606 1987 245
Size: 845 214 302 381 2298
Fragments arranged by size:
2298 845 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 749 1952 749
Size: 1203 2837
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstEII G'GTnAC_C
Cuts at: 219 219
Size: 4040
BstYI r'GATC_y
Cuts at: 1 1030 1065 1532 1555 1890 2136 3148 3234
Size: 1029 35 467 23 335 246 1012 86
Cuts at: 3234 3246 3592 1
Size: 12 346 449
Fragments arranged by size:
1029 1012 467 449 346 335 246 86
35 23 12
Bsu36I CC'TnA_GG
Cuts at: 173 1009 173
Size: 836 3204
BtgI C'CryG_G
Cuts at: 107 848 107
Size: 741 3299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 138 1283 138
Size: 1145 2895
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 251 482 1625 1799 2190 2217 3562 251
Size: 231 1143 174 391 27 1345 729
Fragments arranged by size:
1345 1143 729 391 231 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 205 684 2063 2273 205
Size: 479 1379 210 1972
Fragments arranged by size:
1972 1379 479 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1865 2297 3044 3312 259
Size: 1606 432 747 268 987
Fragments arranged by size:
1606 987 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1000 1000
Size: 4040
EcoO109I rG'GnC_Cy
Cuts at: 1005 1179 1005
Size: 174 3866
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 207 2059 207
Size: 1852 2188
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2744 3723 1474
Size: 248 1022 979 1791
Fragments arranged by size:
1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1882 1912 1914 1944 1882
Size: 30 2 30 3978
Fragments arranged by size:
3978 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
HindIII A'AGCT_T
Cuts at: 1015 1015
Size: 4040
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MfeI C'AATT_G
Cuts at: 932 932
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 253 1801 253
Size: 1548 2492
MslI CAynn'nnrTG
Cuts at: 514 530 850 2029 2156 514
Size: 16 320 1179 127 2398
Fragments arranged by size:
2398 1179 320 127 16
MspA1I CmG'CkG
Cuts at: 446 1151 1465 1825 2838 3083 446
Size: 705 314 360 1013 245 1403
Fragments arranged by size:
1403 1013 705 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NcoI C'CATG_G
Cuts at: 107 107
Size: 4040
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 1093 1593 2500 1093
Size: 500 907 2633
Fragments arranged by size:
2633 907 500
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PmlI CAC'GTG
Cuts at: 924 924
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PstI C_TGCA'G
Cuts at: 1087 1772 1087
Size: 685 3355
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 446 1465 1825 446
Size: 1019 360 2661
Fragments arranged by size:
2661 1019 360
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 684 2063 2273 684
Size: 1379 210 2451
Fragments arranged by size:
2451 1379 210
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SexAI A'CCwGG_T
Cuts at: 215 215
Size: 4040
SfcI C'TryA_G
Cuts at: 33 779 975 1083 1768 2761 2952 33
Size: 746 196 108 685 993 191 1121
Fragments arranged by size:
1121 993 746 685 196 191 108
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 297 787 1195 2110 2602 2864 297
Size: 490 408 915 492 262 1473
Fragments arranged by size:
1473 915 492 490 408 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StyI C'CwwG_G
Cuts at: 107 428 1174 1528 107
Size: 321 746 354 2619
Fragments arranged by size:
2619 746 354 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 386 1623 1756 2215 2424 386
Size: 1237 133 459 209 2002
Fragments arranged by size:
2002 1237 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BsiWI BsmI BsrGI BssHII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI