Restriction map for
pF1KE0891
(Circular) (Six-base) MAPSORT of: pF1KE0891.seq Check: 3702 from: 1 to: 4061
pF1KE0891 4061 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 884 1099 884
Size: 215 3846
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 113 1886 2318 3065 3333 113
Size: 1773 432 747 268 841
Fragments arranged by size:
1773 841 747 432 268
AflII C'TTAA_G
Cuts at: 663 663
Size: 4061
AflIII A'CryG_T
Cuts at: 1413 2517 3494 1413
Size: 1104 977 1980
Fragments arranged by size:
1980 1104 977
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AhdI GACnn_n'nnGTC
Cuts at: 167 167
Size: 4061
AlwNI CAG_nnn'CTG
Cuts at: 805 986 1366 2933 805
Size: 181 380 1567 1933
Fragments arranged by size:
1933 1567 380 181
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 1065 3414 3670 1065
Size: 2349 256 1456
Fragments arranged by size:
2349 1456 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 521 1081 521
Size: 560 3501
BamHI G'GATC_C
Cuts at: 1086 1086
Size: 4061
BanI G'GyrC_C
Cuts at: 491 718 1077 1491 1739 1774 491
Size: 227 359 414 248 35 2778
Fragments arranged by size:
2778 414 359 248 227 35
BanII G_rGCy'C
Cuts at: 1020 1075 2105 1020
Size: 55 1030 2976
Fragments arranged by size:
2976 1030 55
BbeI G_GCGC'C
Cuts at: 722 1495 1743 722
Size: 773 248 3040
Fragments arranged by size:
3040 773 248
BbsI GAAGACnn'nnnn_
Cuts at: 119 442 119
Size: 323 3738
BbvCI CC'TCA_GC
Cuts at: 798 798
Size: 4061
BciVI GTATCCnnnnn_n'
Cuts at: 1959 2720 4061 1959
Size: 761 1341 1959
Fragments arranged by size:
1959 1341 761
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BglI GCCn_nnn'nGGC
Cuts at: 112 259 112
Size: 147 3914
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 347 635 1011 1686 1779 2835 347
Size: 288 376 675 93 1056 1573
Fragments arranged by size:
1573 1056 675 376 288 93
BmgBI CAC'GTC
Cuts at: 570 570
Size: 4061
BmrI ACTGGGnnnn_n'
Cuts at: 1273 1354 1689 3833 1273
Size: 81 335 2144 1501
Fragments arranged by size:
2144 1501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 156 1039 2428 156
Size: 883 1389 1789
Fragments arranged by size:
1789 1389 883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 772
Size: 4061
Bpu10I CC'TnA_GC
Cuts at: 798 798
Size: 4061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2608 2906 1237
Size: 879 492 298 2392
Fragments arranged by size:
2392 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3765 3765
Size: 4061
BsaAI yAC'GTr
Cuts at: 480 1414 480
Size: 934 3127
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 719 1492 1740 2442 3938 719
Size: 773 248 702 1496 842
Fragments arranged by size:
1496 842 773 702 248
BsaWI w'CCGG_w
Cuts at: 1250 1771 2449 2723 2870 3281 1250
Size: 521 678 274 147 411 2030
Fragments arranged by size:
2030 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 559 589 559
Size: 30 4031
BseYI C'CCAG_C
Cuts at: 1486 2244 2821 3317 1486
Size: 758 577 496 2230
Fragments arranged by size:
2230 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2857 104
Size: 1499 46 1208 1308
Fragments arranged by size:
1499 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 679 788 1075 1853 2043 2835 679
Size: 109 287 778 190 792 1905
Fragments arranged by size:
1905 792 778 287 190 109
Bsp1286I G_dGCh'C
Cuts at: 347 635 679 788 1011 1020 1075 1686 1779
Size: 288 44 109 223 9 55 611 93
Cuts at: 1779 1853 2043 2105 2835 347
Size: 74 190 62 730 1573
Fragments arranged by size:
1573 730 611 288 223 190 109 93
74 62 55 44 9
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1111 1325 1627 2008 1111
Size: 214 302 381 3164
Fragments arranged by size:
3164 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 875 1973 875
Size: 1098 2963
BsrFI r'CCGG_y
Cuts at: 2059 2240 2449 2059
Size: 181 209 3671
Fragments arranged by size:
3671 209 181
BsrGI T'GTAC_A
Cuts at: 941 999 941
Size: 58 4003
BssSI C'ACGA_G
Cuts at: 510 672 2332 2690 510
Size: 162 1660 358 1881
Fragments arranged by size:
1881 1660 358 162
BstAPI GCAn_nnn'nTGC
Cuts at: 1438 1438
Size: 4061
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 228 354 366 390 228
Size: 126 12 24 3899
Fragments arranged by size:
3899 126 24 12
BstYI r'GATC_y
Cuts at: 1 1037 1086 1553 1576 1911 2157 3169 3255
Size: 1036 49 467 23 335 246 1012 86
Cuts at: 3255 3267 3613 1
Size: 12 346 449
Fragments arranged by size:
1036 1012 467 449 346 335 246 86
49 23 12
BtgI C'CryG_G
Cuts at: 107 461 107
Size: 354 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 756 1010 2011 2192 2355 756
Size: 254 1001 181 163 2462
Fragments arranged by size:
2462 1001 254 181 163
BtsI GCAGTG_nn'
Cuts at: 731 1304 731
Size: 573 3488
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DraIII CAC_nnn'GTG
Cuts at: 630 630
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2625 1767
Size: 858 3203
EaeI y'GGCC_r
Cuts at: 218 356 458 482 929 1646 1820 2211 2238
Size: 138 102 24 447 717 174 391 27
Cuts at: 2238 3583 218
Size: 1345 696
Fragments arranged by size:
1345 717 696 447 391 174 138 102
27 24
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 865 2084 2294 865
Size: 1219 210 2632
Fragments arranged by size:
2632 1219 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 113 772 1886 2318 3065 3333 113
Size: 659 1114 432 747 268 841
Fragments arranged by size:
1114 841 747 659 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 807 807
Size: 4061
EcoO109I rG'GnC_Cy
Cuts at: 1200 1200
Size: 4061
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 4061
FspI TGC'GCA
Cuts at: 534 1842 534
Size: 1308 2753
HaeII r_GCGC'y
Cuts at: 722 1495 1743 2765 3744 722
Size: 773 248 1022 979 1039
Fragments arranged by size:
1039 1022 979 773 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 153 185 1903 1933 1935 1965 153
Size: 32 1718 30 2 30 2249
Fragments arranged by size:
2249 1718 32 30 30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
KasI G'GCGC_C
Cuts at: 718 1491 1739 718
Size: 773 248 3040
Fragments arranged by size:
3040 773 248
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2732 2916 2732
Size: 184 3877
MscI TGG'CCA
Cuts at: 220 358 484 931 1822 220
Size: 138 126 447 891 2459
Fragments arranged by size:
2459 891 447 138 126
MslI CAynn'nnrTG
Cuts at: 514 937 958 2050 2177 514
Size: 423 21 1092 127 2398
Fragments arranged by size:
2398 1092 423 127 21
MspA1I CmG'CkG
Cuts at: 183 699 778 792 1172 1486 1846 2859 3104
Size: 516 79 14 380 314 360 1013 245
Cuts at: 3104 183
Size: 1140
Fragments arranged by size:
1140 1013 516 380 360 314 245 79
14
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 719 1492 1740 719
Size: 773 248 3040
Fragments arranged by size:
3040 773 248
NcoI C'CATG_G
Cuts at: 107 461 107
Size: 354 3707
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NruI TCG'CGA
Cuts at: 995 995
Size: 4061
NspI r_CATG'y
Cuts at: 1114 1614 2521 1114
Size: 500 907 2654
Fragments arranged by size:
2654 907 500
PasI CC'CwG_GG
Cuts at: 429 429
Size: 4061
PciI A'CATG_T
Cuts at: 2517 2517
Size: 4061
PfoI T'CCnGG_A
Cuts at: 171 171
Size: 4061
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 4029
PstI C_TGCA'G
Cuts at: 1108 1793 1108
Size: 685 3376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 699 778 792 1486 1846 699
Size: 79 14 694 360 2914
Fragments arranged by size:
2914 694 360 79 14
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 947 978 1104 1789 2782 2973 33
Size: 914 31 126 685 993 191 1121
Fragments arranged by size:
1121 993 914 685 191 126 31
SfoI GGC'GCC
Cuts at: 720 1493 1741 720
Size: 773 248 3040
Fragments arranged by size:
3040 773 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 523 1083 523
Size: 560 3501
SmlI C'TyrA_G
Cuts at: 663 1216 2131 2623 2885 663
Size: 553 915 492 262 1839
Fragments arranged by size:
1839 915 553 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 107 461 1195 1549 107
Size: 354 734 354 2619
Fragments arranged by size:
2619 734 354 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 297 700 1644 1777 2236 2445 297
Size: 403 944 133 459 209 1913
Fragments arranged by size:
1913 944 459 403 209 133
TatI w'GTAC_w
Cuts at: 287 413 941 999 3576 287
Size: 126 528 58 2577 772
Fragments arranged by size:
2577 772 528 126 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 230 262 559 591 3578 3610 230
Size: 32 297 32 2987 32 681
Fragments arranged by size:
2987 681 297 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1556 1556
Size: 4061
XmaI C'CCGG_G
Cuts at: 521 1081 521
Size: 560 3501
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmtI BseRI BsgI BsiWI
BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI