Restriction map for
pF1KE0888
(Circular) (Six-base) MAPSORT of: pF1KE0888.seq Check: 5890 from: 1 to: 4178
pF1KE0888 4178 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 595 595
Size: 4178
AccI GT'mk_AC
Cuts at: 1216 1216
Size: 4178
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 2003 2435 3182 3450 682
Size: 1321 432 747 268 1410
Fragments arranged by size:
1410 1321 747 432 268
AflIII A'CryG_T
Cuts at: 1530 2634 3611 1530
Size: 1104 977 2097
Fragments arranged by size:
2097 1104 977
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AleI CACnn'nnGTG
Cuts at: 847 847
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 1483 3050 1483
Size: 1567 2611
ApaLI G'TGCA_C
Cuts at: 2948 2948
Size: 4178
ApoI r'AATT_y
Cuts at: 139 1182 3531 3787 139
Size: 1043 2349 256 530
Fragments arranged by size:
2349 1043 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 800 1198 800
Size: 398 3780
AvrII C'CTAG_G
Cuts at: 1045 1045
Size: 4178
BamHI G'GATC_C
Cuts at: 1203 1203
Size: 4178
BanI G'GyrC_C
Cuts at: 727 1194 1608 1856 1891 727
Size: 467 414 248 35 3014
Fragments arranged by size:
3014 467 414 248 35
BanII G_rGCy'C
Cuts at: 1192 2222 1192
Size: 1030 3148
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2837 4178 2076
Size: 761 1341 2076
Fragments arranged by size:
2076 1341 761
BclI T'GATC_A
Cuts at: 557 1698 557
Size: 1141 3037
BfrBI ATG'CAT
Cuts at: 1116 1116
Size: 4178
BglII A'GATC_T
Cuts at: 1693 3730 1693
Size: 2037 2141
BlpI GC'TnA_GC
Cuts at: 1290 1290
Size: 4178
Bme1580I G_kGCm'C
Cuts at: 730 1803 1896 2952 730
Size: 1073 93 1056 1956
Fragments arranged by size:
1956 1073 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1390 1471 1806 3950 1390
Size: 81 335 2144 1618
Fragments arranged by size:
2144 1618 335 81
BmtI G_CTAG'C
Cuts at: 355 355
Size: 4178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 553 1154 133
Size: 420 601 3157
Fragments arranged by size:
3157 601 420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1354 2233 2725 3023 1354
Size: 879 492 298 2509
Fragments arranged by size:
2509 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3882 3882
Size: 4178
BsaAI yAC'GTr
Cuts at: 1531 1531
Size: 4178
BsaBI GATnn'nnATC
Cuts at: 6 1070 1692 3729 6
Size: 1064 622 2037 455
Fragments arranged by size:
2037 1064 622 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1367 1888 2566 2840 2987 3398 1367
Size: 521 678 274 147 411 2147
Fragments arranged by size:
2147 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 897 1151 175
Size: 722 254 3202
Fragments arranged by size:
3202 722 254
BseYI C'CCAG_C
Cuts at: 540 901 1153 1603 2361 2938 3434 540
Size: 361 252 450 758 577 496 1284
Fragments arranged by size:
1284 758 577 496 450 361 252
BsiEI CG_ry'CG
Cuts at: 104 471 1720 1766 2974 104
Size: 367 1249 46 1208 1308
Fragments arranged by size:
1308 1249 1208 367 46
BsiHKAI G_wGCw'C
Cuts at: 520 1192 1970 2160 2952 520
Size: 672 778 190 792 1746
Fragments arranged by size:
1746 792 778 672 190
Bsp1286I G_dGCh'C
Cuts at: 520 730 1192 1803 1896 1970 2160 2222 2952
Size: 210 462 611 93 74 190 62 730
Cuts at: 2952 520
Size: 1746
Fragments arranged by size:
1746 730 611 462 210 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 3354 3354
Size: 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 595 1228 1442 1744 2125 595
Size: 633 214 302 381 2648
Fragments arranged by size:
2648 633 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2470 2524 4008 4173 50
Size: 2420 54 1484 165 55
Fragments arranged by size:
2420 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2090 2090
Size: 4178
BsrFI r'CCGG_y
Cuts at: 616 2176 2357 2566 616
Size: 1560 181 209 2228
Fragments arranged by size:
2228 1560 209 181
BssSI C'ACGA_G
Cuts at: 2449 2807 2449
Size: 358 3820
BstAPI GCAn_nnn'nTGC
Cuts at: 1555 1555
Size: 4178
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstEII G'GTnAC_C
Cuts at: 216 216
Size: 4178
BstXI CCAn_nnnn'nTGG
Cuts at: 213 696 213
Size: 483 3695
BstYI r'GATC_y
Cuts at: 1 788 1203 1670 1693 2028 2274 3286 3372
Size: 787 415 467 23 335 246 1012 86
Cuts at: 3372 3384 3730 1
Size: 12 346 449
Fragments arranged by size:
1012 787 467 449 415 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 434 107
Size: 327 3851
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2128 2309 2472 2128
Size: 181 163 3834
Fragments arranged by size:
3834 181 163
BtsI GCAGTG_nn'
Cuts at: 1421 1421
Size: 4178
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 248 461 479 1031 1763 1937 2328 2355 3700
Size: 213 18 552 732 174 391 27 1345
Cuts at: 3700 248
Size: 726
Fragments arranged by size:
1345 732 726 552 391 213 174 27
18
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 193 301 2201 2411 193
Size: 108 1900 210 1960
Fragments arranged by size:
1960 1900 210 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1190 1190
Size: 4178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 553 682 1154 2003 2435 3182 3450 133
Size: 420 129 472 849 432 747 268 861
Fragments arranged by size:
861 849 747 472 432 420 268 129
EcoNI CCTnn'n_nnAGG
Cuts at: 1044 1044
Size: 4178
EcoO109I rG'GnC_Cy
Cuts at: 1317 1317
Size: 4178
EcoRI G'AATT_C
Cuts at: 139 1182 139
Size: 1043 3135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 4178
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 1612 1860 2882 3861 1612
Size: 248 1022 979 1929
Fragments arranged by size:
1929 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2020 2050 2052 2082 2020
Size: 30 2 30 4116
Fragments arranged by size:
4116 30 30 2
HincII GTy'rAC
Cuts at: 315 1217 315
Size: 902 3276
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3033 2849
Size: 184 3994
MscI TGG'CCA
Cuts at: 250 463 481 1939 250
Size: 213 18 1458 2489
Fragments arranged by size:
2489 1458 213 18
MslI CAynn'nnrTG
Cuts at: 280 406 721 847 955 1111 2167 2294 280
Size: 126 315 126 108 156 1056 127 2164
Fragments arranged by size:
2164 1056 315 156 127 126 126 108
MspA1I CmG'CkG
Cuts at: 1289 1603 1963 2976 3221 1289
Size: 314 360 1013 245 2246
Fragments arranged by size:
2246 1013 360 314 245
NaeI GCC'GGC
Cuts at: 618 2359 618
Size: 1741 2437
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 107 434 107
Size: 327 3851
NgoMIV G'CCGG_C
Cuts at: 616 2357 616
Size: 1741 2437
NheI G'CTAG_C
Cuts at: 351 351
Size: 4178
NsiI A_TGCA'T
Cuts at: 1118 1118
Size: 4178
NspI r_CATG'y
Cuts at: 1231 1731 2638 1231
Size: 500 907 2771
Fragments arranged by size:
2771 907 500
PasI CC'CwG_GG
Cuts at: 1008 1008
Size: 4178
PciI A'CATG_T
Cuts at: 2634 2634
Size: 4178
PfoI T'CCnGG_A
Cuts at: 1080 1080
Size: 4178
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PstI C_TGCA'G
Cuts at: 1225 1910 1225
Size: 685 3493
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 1603 1963 1603
Size: 360 3818
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 1192 1192
Size: 4178
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 2201 2411 2201
Size: 210 3968
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SexAI A'CCwGG_T
Cuts at: 212 212
Size: 4178
SfcI C'TryA_G
Cuts at: 33 975 1221 1906 2899 3090 33
Size: 942 246 685 993 191 1121
Fragments arranged by size:
1121 993 942 685 246 191
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 1333 2248 2740 3002 1333
Size: 915 492 262 2509
Fragments arranged by size:
2509 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
StyI C'CwwG_G
Cuts at: 107 434 1045 1312 1666 107
Size: 327 611 267 354 2619
Fragments arranged by size:
2619 611 354 327 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 418 1761 1894 2353 2562 294
Size: 124 1343 133 459 209 1910
Fragments arranged by size:
1910 1343 459 209 133 124
TatI w'GTAC_w
Cuts at: 284 881 3693 284
Size: 597 2812 769
Fragments arranged by size:
2812 769 597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4146
Tth111I GACn'n_nGTC
Cuts at: 1975 1975
Size: 4178
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 1673 387
Size: 1286 2892
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
XmnI GAAnn'nnTTC
Cuts at: 817 817
Size: 4178
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PasI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
BaeI BbsI BbvCI BcgI BglI BmgBI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI PacI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI