Restriction map for
pF1KE0885
(Circular) (Six-base) MAPSORT of: pF1KE0885.seq Check: 8226 from: 1 to: 4103
pF1KE0885 4103 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 332 547 332
Size: 215 3888
AccI GT'mk_AC
Cuts at: 1141 1141
Size: 4103
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1928 2360 3107 3375 1928
Size: 432 747 268 2656
Fragments arranged by size:
2656 747 432 268
AflIII A'CryG_T
Cuts at: 701 1455 2559 3536 701
Size: 754 1104 977 1268
Fragments arranged by size:
1268 1104 977 754
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 403 435 403
Size: 32 4071
AlwNI CAG_nnn'CTG
Cuts at: 240 1408 2975 240
Size: 1168 1567 1368
Fragments arranged by size:
1567 1368 1168
ApaLI G'TGCA_C
Cuts at: 2873 2873
Size: 4103
ApoI r'AATT_y
Cuts at: 1107 3456 3712 1107
Size: 2349 256 1498
Fragments arranged by size:
2349 1498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 674 1123 674
Size: 449 3654
BamHI G'GATC_C
Cuts at: 1128 1128
Size: 4103
BanI G'GyrC_C
Cuts at: 1119 1533 1781 1816 1119
Size: 414 248 35 3406
Fragments arranged by size:
3406 414 248 35
BanII G_rGCy'C
Cuts at: 574 581 1059 1117 2147 574
Size: 7 478 58 1030 2530
Fragments arranged by size:
2530 1030 478 58 7
BbeI G_GCGC'C
Cuts at: 1537 1785 1537
Size: 248 3855
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 1623 1623
Size: 4103
BfrBI ATG'CAT
Cuts at: 1050 1050
Size: 4103
BglI GCCn_nnn'nGGC
Cuts at: 112 514 112
Size: 402 3701
BglII A'GATC_T
Cuts at: 1618 3655 1618
Size: 2037 2066
BlpI GC'TnA_GC
Cuts at: 1215 1215
Size: 4103
Bme1580I G_kGCm'C
Cuts at: 117 1728 1821 2877 117
Size: 1611 93 1056 1343
Fragments arranged by size:
1611 1343 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1315 1396 1731 3875 1315
Size: 81 335 2144 1543
Fragments arranged by size:
2144 1543 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
Bpu10I CC'TnA_GC
Cuts at: 224 224
Size: 4103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1279 2158 2650 2948 1279
Size: 879 492 298 2434
Fragments arranged by size:
2434 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 480 909 1071 3807 480
Size: 429 162 2736 776
Fragments arranged by size:
2736 776 429 162
BsaAI yAC'GTr
Cuts at: 820 1456 820
Size: 636 3467
BsaBI GATnn'nnATC
Cuts at: 6 1617 3654 6
Size: 1611 2037 455
Fragments arranged by size:
2037 1611 455
BsaHI Gr'CG_yC
Cuts at: 1534 1782 2484 3980 1534
Size: 248 702 1496 1657
Fragments arranged by size:
1657 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1292 1813 2491 2765 2912 3323 1292
Size: 521 678 274 147 411 2072
Fragments arranged by size:
2072 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 622 625 583
Size: 39 3 4061
Fragments arranged by size:
4061 39 3
BseYI C'CCAG_C
Cuts at: 279 1528 2286 2863 3359 279
Size: 1249 758 577 496 1023
Fragments arranged by size:
1249 1023 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 310
Size: 4103
BsiEI CG_ry'CG
Cuts at: 104 1645 1691 2899 104
Size: 1541 46 1208 1308
Fragments arranged by size:
1541 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 293 1117 1895 2085 2877 293
Size: 824 778 190 792 1519
Fragments arranged by size:
1519 824 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 117 293 574 581 1059 1117 1728 1821 1895
Size: 176 281 7 478 58 611 93 74
Cuts at: 1895 2085 2147 2877 117
Size: 190 62 730 1343
Fragments arranged by size:
1343 730 611 478 281 190 176 93
74 62 58 7
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 800 3279 800
Size: 2479 1624
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 547 947 1153 1367 1669 2050 332
Size: 215 400 206 214 302 381 2385
Fragments arranged by size:
2385 400 381 302 215 214 206
BsrBI CCG'CTC
Cuts at: 50 2395 2449 3933 4098 50
Size: 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2015 2015
Size: 4103
BsrFI r'CCGG_y
Cuts at: 2101 2282 2491 2101
Size: 181 209 3713
Fragments arranged by size:
3713 209 181
BsrGI T'GTAC_A
Cuts at: 704 704
Size: 4103
BssSI C'ACGA_G
Cuts at: 2374 2732 2374
Size: 358 3745
BstAPI GCAn_nnn'nTGC
Cuts at: 1480 1480
Size: 4103
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstEII G'GTnAC_C
Cuts at: 483 657 483
Size: 174 3929
BstXI CCAn_nnnn'nTGG
Cuts at: 267 405 267
Size: 138 3965
BstYI r'GATC_y
Cuts at: 1 209 236 680 1128 1595 1618 1953 2199
Size: 208 27 444 448 467 23 335 246
Cuts at: 2199 3211 3297 3309 3655 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 448 444 346 335 246
208 86 27 23 12
Bsu36I CC'TnA_GG
Cuts at: 792 1033 792
Size: 241 3862
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2053 2234 2397 2053
Size: 181 163 3759
Fragments arranged by size:
3759 181 163
BtsI GCAGTG_nn'
Cuts at: 1346 1346
Size: 4103
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DrdI GACnn_nn'nnGTC
Cuts at: 1809 2667 1809
Size: 858 3245
EaeI y'GGCC_r
Cuts at: 302 350 506 533 1688 1862 2253 2280 3625
Size: 48 156 27 1155 174 391 27 1345
Cuts at: 3625 302
Size: 780
Fragments arranged by size:
1345 1155 780 391 174 156 48 27
27
EagI C'GGCC_G
Cuts at: 1688 1688
Size: 4103
EarI CTCTTCn'nnn_
Cuts at: 379 697 1059 2126 2336 379
Size: 318 362 1067 210 2146
Fragments arranged by size:
2146 1067 362 318 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2311 2621 2767 3913 2311
Size: 310 146 1146 2501
Fragments arranged by size:
2501 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1115 1115
Size: 4103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1928 2360 3107 3375 1928
Size: 432 747 268 2656
Fragments arranged by size:
2656 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1086 1086
Size: 4103
EcoO109I rG'GnC_Cy
Cuts at: 1242 1242
Size: 4103
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 4103
FspI TGC'GCA
Cuts at: 1884 1884
Size: 4103
HaeII r_GCGC'y
Cuts at: 1537 1785 2807 3786 1537
Size: 248 1022 979 1854
Fragments arranged by size:
1854 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1945 1975 1977 2007 1945
Size: 30 2 30 4041
Fragments arranged by size:
4041 30 30 2
HincII GTy'rAC
Cuts at: 1142 1142
Size: 4103
KasI G'GCGC_C
Cuts at: 1533 1781 1533
Size: 248 3855
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 755 2774 2958 755
Size: 2019 184 1900
Fragments arranged by size:
2019 1900 184
MscI TGG'CCA
Cuts at: 304 352 535 1864 304
Size: 48 183 1329 2543
Fragments arranged by size:
2543 1329 183 48
MslI CAynn'nnrTG
Cuts at: 334 565 896 985 2092 2219 334
Size: 231 331 89 1107 127 2218
Fragments arranged by size:
2218 1107 331 231 127 89
MspA1I CmG'CkG
Cuts at: 1214 1528 1888 2901 3146 1214
Size: 314 360 1013 245 2171
Fragments arranged by size:
2171 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 4103
NarI GG'CG_CC
Cuts at: 1534 1782 1534
Size: 248 3855
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 4103
NsiI A_TGCA'T
Cuts at: 1052 1052
Size: 4103
NspI r_CATG'y
Cuts at: 705 728 1050 1156 1656 2563 705
Size: 23 322 106 500 907 2245
Fragments arranged by size:
2245 907 500 322 106 23
PasI CC'CwG_GG
Cuts at: 574 574
Size: 4103
PciI A'CATG_T
Cuts at: 701 2559 701
Size: 1858 2245
PfoI T'CCnGG_A
Cuts at: 1059 1059
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PstI C_TGCA'G
Cuts at: 944 1150 1835 944
Size: 206 685 3212
Fragments arranged by size:
3212 685 206
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 1528 1888 1528
Size: 360 3743
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 1117 1117
Size: 4103
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SapI GCTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SexAI A'CCwGG_T
Cuts at: 266 266
Size: 4103
SfcI C'TryA_G
Cuts at: 33 940 1026 1146 1831 2824 3015 33
Size: 907 86 120 685 993 191 1121
Fragments arranged by size:
1121 993 907 685 191 120 86
SfoI GGC'GCC
Cuts at: 1535 1783 1535
Size: 248 3855
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 1125 1125
Size: 4103
SmlI C'TyrA_G
Cuts at: 674 1258 2173 2665 2927 674
Size: 584 915 492 262 1850
Fragments arranged by size:
1850 915 584 492 262
SnaBI TAC'GTA
Cuts at: 820 820
Size: 4103
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 1156 1656 1156
Size: 500 3603
StuI AGG'CCT
Cuts at: 1092 1092
Size: 4103
StyI C'CwwG_G
Cuts at: 479 1237 1591 479
Size: 758 354 2991
Fragments arranged by size:
2991 758 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 751 886 1686 1819 2278 2487 751
Size: 135 800 133 459 209 2367
Fragments arranged by size:
2367 800 459 209 135 133
TatI w'GTAC_w
Cuts at: 338 704 3618 338
Size: 366 2914 823
Fragments arranged by size:
2914 823 366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4071
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1598 1598
Size: 4103
XhoI C'TCGA_G
Cuts at: 674 674
Size: 4103
XmaI C'CCGG_G
Cuts at: 1123 1123
Size: 4103
XmnI GAAnn'nnTTC
Cuts at: 1018 1018
Size: 4103
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PasI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BmgBI BmtI BpmI
BsaXI BsiWI BsmI BsmBI BssHII BstZ17I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI PacI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI SspI SwaI ZraI