Restriction map for
pF1KE0884
(Circular) (Six-base) MAPSORT of: pF1KE0884.seq Check: 45 from: 1 to: 4049
pF1KE0884 4049 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 881 1087 881
Size: 206 3843
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
AflIII A'CryG_T
Cuts at: 1401 2505 3482 1401
Size: 1104 977 1968
Fragments arranged by size:
1968 1104 977
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AleI CACnn'nnGTG
Cuts at: 223 280 223
Size: 57 3992
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 349 381 657 689 349
Size: 32 276 32 3709
Fragments arranged by size:
3709 276 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1354 2921 1354
Size: 1567 2482
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 1053 3402 3658 1053
Size: 2349 256 1444
Fragments arranged by size:
2349 1444 256
AseI AT'TA_AT
Cuts at: 20 689 20
Size: 669 3380
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 1069 1069
Size: 4049
BamHI G'GATC_C
Cuts at: 182 1074 182
Size: 892 3157
BanI G'GyrC_C
Cuts at: 1065 1479 1727 1762 1065
Size: 414 248 35 3352
Fragments arranged by size:
3352 414 248 35
BanII G_rGCy'C
Cuts at: 520 1005 1063 2093 520
Size: 485 58 1030 2476
Fragments arranged by size:
2476 1030 485 58
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BbsI GAAGACnn'nnnn_
Cuts at: 1017 1017
Size: 4049
BbvCI CC'TCA_GC
Cuts at: 661 661
Size: 4049
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 749 1569 749
Size: 820 3229
BfrBI ATG'CAT
Cuts at: 996 996
Size: 4049
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 1674 1767 2823 1674
Size: 93 1056 2900
Fragments arranged by size:
2900 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1261 1342 1677 3821 1261
Size: 81 335 2144 1489
Fragments arranged by size:
2144 1489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 799 2416 799
Size: 1617 2432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 346
Size: 4049
Bpu10I CC'TnA_GC
Cuts at: 170 521 661 170
Size: 351 140 3558
Fragments arranged by size:
3558 351 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2104 2596 2894 1225
Size: 879 492 298 2380
Fragments arranged by size:
2380 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 426 3753 426
Size: 3327 722
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 1480 1728 2430 3926 1480
Size: 248 702 1496 1603
Fragments arranged by size:
1603 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1238 1759 2437 2711 2858 3269 1238
Size: 521 678 274 147 411 2018
Fragments arranged by size:
2018 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 572 602 572
Size: 30 4019
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 580
Size: 4049
BseYI C'CCAG_C
Cuts at: 1474 2232 2809 3305 1474
Size: 758 577 496 2218
Fragments arranged by size:
2218 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 866 256
Size: 610 3439
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 239 1063 1841 2031 2823 239
Size: 824 778 190 792 1465
Fragments arranged by size:
1465 824 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 239 520 1005 1063 1674 1767 1841 2031 2093
Size: 281 485 58 611 93 74 190 62
Cuts at: 2093 2823 239
Size: 730 1465
Fragments arranged by size:
1465 730 611 485 281 190 93 74
62 58
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 593 3225 593
Size: 2632 1417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1099 1313 1615 1996 1099
Size: 214 302 381 3152
Fragments arranged by size:
3152 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1961 1961
Size: 4049
BsrFI r'CCGG_y
Cuts at: 2047 2228 2437 2047
Size: 181 209 3659
Fragments arranged by size:
3659 209 181
BssSI C'ACGA_G
Cuts at: 2320 2678 2320
Size: 358 3691
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstXI CCAn_nnnn'nTGG
Cuts at: 213 351 213
Size: 138 3911
BstYI r'GATC_y
Cuts at: 1 182 1074 1541 1564 1899 2145 3157 3243
Size: 181 892 467 23 335 246 1012 86
Cuts at: 3243 3255 3601 1
Size: 12 346 449
Fragments arranged by size:
1012 892 467 449 346 335 246 181
86 23 12
BstZ17I GTA'TAC
Cuts at: 882 882
Size: 4049
Bsu36I CC'TnA_GG
Cuts at: 979 979
Size: 4049
BtgI C'CryG_G
Cuts at: 107 107
Size: 4049
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 1292 1292
Size: 4049
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DraIII CAC_nnn'GTG
Cuts at: 699 699
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 248 296 479 1634 1808 2199 2226 3571 248
Size: 48 183 1155 174 391 27 1345 726
Fragments arranged by size:
1345 1155 726 391 183 174 48 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 325 862 2072 2282 325
Size: 537 1210 210 2092
Fragments arranged by size:
2092 1210 537 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1874 2306 3053 3321 346
Size: 1528 432 747 268 1074
Fragments arranged by size:
1528 1074 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 555 1188 555
Size: 633 3416
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 1483 1731 2753 3732 1483
Size: 248 1022 979 1800
Fragments arranged by size:
1800 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 734 766 1891 1921 1923 1953 734
Size: 32 1125 30 2 30 2830
Fragments arranged by size:
2830 1125 32 30 30 2
HincII GTy'rAC
Cuts at: 1088 1088
Size: 4049
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 701 2720 2904 701
Size: 2019 184 1846
Fragments arranged by size:
2019 1846 184
MscI TGG'CCA
Cuts at: 250 298 481 1810 250
Size: 48 183 1329 2489
Fragments arranged by size:
2489 1329 183 48
MslI CAynn'nnrTG
Cuts at: 223 280 511 842 931 2038 2165 223
Size: 57 231 331 89 1107 127 2107
Fragments arranged by size:
2107 1107 331 231 127 89 57
MspA1I CmG'CkG
Cuts at: 180 1160 1474 1834 2847 3092 180
Size: 980 314 360 1013 245 1137
Fragments arranged by size:
1137 1013 980 360 314 245
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NcoI C'CATG_G
Cuts at: 107 107
Size: 4049
NdeI CA'TA_TG
Cuts at: 465 465
Size: 4049
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NsiI A_TGCA'T
Cuts at: 998 998
Size: 4049
NspI r_CATG'y
Cuts at: 674 996 1102 1602 2509 674
Size: 322 106 500 907 2214
Fragments arranged by size:
2214 907 500 322 106
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 117 149 3188 3220 117
Size: 32 3039 32 946
Fragments arranged by size:
3039 946 32 32
PpuMI rG'GwC_Cy
Cuts at: 555 555
Size: 4049
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 1474 1834 1474
Size: 360 3689
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SexAI A'CCwGG_T
Cuts at: 212 212
Size: 4049
SfcI C'TryA_G
Cuts at: 33 972 1092 1777 2770 2961 33
Size: 939 120 685 993 191 1121
Fragments arranged by size:
1121 993 939 685 191 120
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 1071 1071
Size: 4049
SmlI C'TyrA_G
Cuts at: 1204 2119 2611 2873 1204
Size: 915 492 262 2380
Fragments arranged by size:
2380 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
StyI C'CwwG_G
Cuts at: 107 425 1183 1537 107
Size: 318 758 354 2619
Fragments arranged by size:
2619 758 354 318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 832 966 1632 1765 2224 2433 832
Size: 134 666 133 459 209 2448
Fragments arranged by size:
2448 666 459 209 134 133
TatI w'GTAC_w
Cuts at: 284 3564 284
Size: 3280 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 611 643 3566 3598 611
Size: 32 2923 32 1062
Fragments arranged by size:
2923 1062 32 32
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 1069 1069
Size: 4049
XmnI GAAnn'nnTTC
Cuts at: 964 964
Size: 4049
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI